EN 17805:2023

Water quality - Sampling, capture and preservation of environmental DNA from water

This document specifies procedures for sampling, capture and preservation of environmental DNA (eDNA) in aquatic environments, stemming from organisms that are or have recently been present in a waterbody, have visited it or whose DNA has been introduced to the waterbody through some mechanism. This document also covers procedures for avoiding sample contamination and ensuring DNA quality, key properties of the filtering procedure and equipment and reporting standards.
This document does not include the collection of eDNA from biofilms, sediments or similar sample types and does not cover sampling designs.

Wasserbeschaffenheit - Probenahme, Erfassung und Konservierung von Umwelt‑DNA in Wasser

Dieses Dokument legt Verfahren für die Probenahme, den Fang und die Konservierung von Umwelt DNA (eDNA) in Gewässern fest, die von Organismen stammen, die sich in einem Gewässer aufhalten oder aufgehalten haben, das Gewässer besucht haben oder deren DNA durch irgendeinen Mechanismus in das Gewässer gelangt ist. Dieses Dokument behandelt auch Verfahren zur Vermeidung von Proben¬kontaminationen und zur Sicherstellung der DNA Qualität, Schlüsseleigenschaften des Filtrationsverfahrens und der Ausrüstung sowie Berichtsstandards.
Dieses Dokument befasst sich nicht mit der Gewinnung von eDNA aus Biofilmen, Sedimenten oder ähnlichen Probenarten und geht nicht auf die Gestaltung der Probenahme ein.

Qualité de l'eau - Échantillonnage, collecte et conservation de l'ADN environnemental prélevé dans l'eau

Le présent document spécifie des modes opératoires d'échantillonnage, de capture et de conservation de l’ADN environnemental (ADNe) dans des milieux aquatiques, provenant d’organismes qui sont ou qui ont été récemment présents dans une masse d’eau, qui l’ont parcouru ou dont l’ADN a été introduit dans la masse d’eau par un mécanisme précis. Le présent document porte également sur les modes opératoires permettant d'éviter la contamination des échantillons et de contrôler la qualité de l’ADN, les principales propriétés du mode opératoire et de l'équipement de filtration et les normes relatives au compte rendu
Le présent document n’inclut pas la collecte d’ADNe de biofilms, sédiments ou autres types d'échantillons similaires et n’aborde pas la question des plans d'échantillonnage.

Kakovost vode - Vzorčenje, zbiranje in konzerviranje okoljske DNK iz vode

Vzorčenje vode za zajem okoljske DNK (eDNA) v vodnih okoljih. Okoljski DNK izvira iz organizmov, ki živijo ali so pred kratkim živeli v vodnem telesu, in ne vključuje okoljskega DNK, ki se nahaja v usedlinah ali podobnih vrstah vzorcev. Standard zajema postopke za preprečevanje kontaminacije vzorcev in zagotavljanje kakovosti DNK, ključne lastnosti postopka filtriranja in opreme ter standarde za poročanje.

General Information

• Status: Published
• Publication Date: 14-Mar-2023
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230/WG 28 - DNA and eDNA methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Mar-2023
• Due Date: 02-Feb-2023
• Completion Date: 15-Mar-2023
• Directive: 2000/60/EC - Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy (substitutes Directive Reference 01/991 - Common Position on WFD)
• Ref Project: SIST EN 17805:2023 - Water quality - Sampling, capture and preservation of environmental DNA from water

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17805:2023
01-junij-2023
Kakovost vode - Vzorčenje, zbiranje in konzerviranje okoljske DNK iz vode
Water quality - Sampling, capture and preservation of environmental DNA from water
Wasserbeschaffenheit - Probenahme, Erfassung und Konservierung von Umwelt‑DNA
in Wasser
Qualité de l'eau - Échantillonnage, collecte et conservation de l'ADN environnemental
prélevé dans l'eau
Ta slovenski standard je istoveten z: EN 17805:2023
ICS:
13.060.45 Preiskava vode na splošno Examination of water in
general
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN 17805:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 17805:2023

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SIST EN 17805:2023


EN 17805
EUROPEAN STANDARD

NORME EUROPÉENNE

March 2023
EUROPÄISCHE NORM
ICS 13.060.70
English Version

Water quality - Sampling, capture and preservation of
environmental DNA from water
Qualité de l'eau - Échantillonnage, collecte et Wasserbeschaffenheit - Probenahme, Erfassung und
conservation de l'ADN environnemental prélevé dans Konservierung von Umwelt-DNA in Wasser
l'eau
This European Standard was approved by CEN on 30 January 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17805:2023 E
worldwide for CEN national Members.

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SIST EN 17805:2023
EN 17805:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 7
5 Procedure. 8
5.1 General . 8
5.2 Considerations prior to fieldwork . 8
5.3 Equipment preparation prior to fieldwork . 8
5.4 Sampling the eDNA from water . 8
5.5 Preserving the sample . 9
6 Equipment .
...