Animal and vegetable fats and oils - Determination of benzo[a]pyrene - Reverse-phase high performance liquid chromatography method (ISO 15302:2017)

ISO 15302:2017 specifies a method for the determination of benzo[a]pyrene in crude or refined edible oils and fats by reverse-phase high performance liquid chromatography (HPLC) using fluorimetric detection in the range 0,1 µg/kg to 50 µg/kg.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Tierische und pflanzliche Fette und Öle - Bestimmung des Benzo[a]pyren-Gehalts - Umkehrphasen-Hochleistungsflüssigchromatographisches Verfahren (ISO 15302:2017)

Corps gras d'origines animale et végétale - Détermination du benzo[a]pyrène - Méthode par chromatographie liquide à haute performance à polarité de phase inversée (ISO 15302:2017)

ISO 15302:2017 spécifie une méthode de détermination du benzo[a]pyrène dans les corps gras comestibles bruts ou raffinés, par chromatographie liquide à haute performance (CLHP) à polarité de phase inversée, à l'aide d'une détection fluorimétrique dans la gamme de 0,1 µg/kg à 50 µg/kg.
Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application du présent document.

Živalske in rastlinske maščobe ter olja - Določevanje benzo[a]pirena - Metoda tekočinske kromatografije visoke ločljivosti z reverzno fazo (ISO 15302:2017)

Ta dokument določa metodo za določevanje benzo[a]pirena v surovih ali rafiniranih užitnih oljih in maščobah s tekočinsko kromatografijo visoke ločljivosti z reverzno fazo z uporabo fluorimetrične detekcije v razponu 0,1–50 μg/kg.
Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem
dokumentu.

General Information

Status
Published
Publication Date
25-Apr-2017
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Due Date
26-Apr-2017
Completion Date
26-Apr-2017

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SLOVENSKI STANDARD
SIST EN ISO 15302:2017
01-julij-2017
1DGRPHãþD
SIST EN ISO 15302:2010
äLYDOVNHLQUDVWOLQVNHPDãþREHWHUROMD'RORþHYDQMHEHQ]R>D@SLUHQD0HWRGD
WHNRþLQVNHNURPDWRJUDILMHYLVRNHORþOMLYRVWL]UHYHU]QRID]R ,62

Animal and vegetable fats and oils - Determination of benzo[a]pyrene - Reverse-phase

high performance liquid chromatography method (ISO 15302:2017)
Tierische und pflanzliche Fette und Öle - Bestimmung des Benzo[a]pyren-Gehalts -
Umkehrphasen-Hochleistungsflüssigchromatographisches Verfahren (ISO 15302:2017)

Corps gras d'origines animale et végétale - Détermination du benzo[a]pyrène - Méthode

par chromatographie liquide à haute performance à polarité de phase inversée (ISO

15302:2017)
Ta slovenski standard je istoveten z: EN ISO 15302:2017
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN ISO 15302:2017 en

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 15302:2017
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SIST EN ISO 15302:2017
EN ISO 15302
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2017
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 15302:2010
English Version
Animal and vegetable fats and oils - Determination of
benzo[a]pyrene - Reverse-phase high performance liquid
chromatography method (ISO 15302:2017)

Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung

Détermination du benzo[a]pyrène - Méthode par des Benzo[a]pyren-Gehalts - Umkehrphasen-

chromatographie liquide à haute performance à Hochleistungsflüssigchromatographisches Verfahren

polarité de phase inversée (ISO 15302:2017) (ISO 15302:2017)
This European Standard was approved by CEN on 13 April 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,

Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15302:2017 E

worldwide for CEN national Members.
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SIST EN ISO 15302:2017
EN ISO 15302:2017 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

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SIST EN ISO 15302:2017
EN ISO 15302:2017 (E)
European foreword

This document (EN ISO 15302:2017) has been prepared by Technical Committee ISO/TC 34 “Food

products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats

and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by

AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by October 2017, and conflicting national standards shall

be withdrawn at the latest by October 2017.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent

rights.
This document supersedes EN ISO 15302:2010.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,

France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,

Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,

Turkey and the United Kingdom.
Endorsement notice

The text of ISO 15302:2017 has been approved by CEN as EN ISO 15302:2017 without any modification.

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SIST EN ISO 15302:2017
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SIST EN ISO 15302:2017
INTERNATIONAL ISO
STANDARD 15302
Third edition
2017-03
Animal and vegetable fats and oils
— Determination of benzo[a]pyrene
— Reverse-phase high performance
liquid chromatography method
Corps gras d’origines animale et végétale — Détermination du
benzo[a]pyrène — Méthode par chromatographie liquide à haute
performance à polarité de phase inversée
Reference number
ISO 15302:2017(E)
ISO 2017
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SIST EN ISO 15302:2017
ISO 15302:2017(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2017, Published in Switzerland

All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form

or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior

written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of

the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2017 – All rights reserved
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SIST EN ISO 15302:2017
ISO 15302:2017(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 1

5 Reagents ........................................................................................................................................................................................................................ 1

6 Apparatus ..................................................................................................................................................................................................................... 3

7 Sampling ........................................................................................................................................................................................................................ 4

8 Preparation of test sample ......................................................................................................................................................................... 4

9 Procedure..................................................................................................................................................................................................................... 4

10 Expression of results ........................................................................................................................................................................................ 6

10.1 Calculation of relative response factors ............................................................................................................................ 6

10.2 Calculation of benzo[a]pyrene content ............................................................................................................................. 7

11 Precision ....................................................................................................................................................................................................................... 7

11.1 Repeatability ............................................................................................................................................................................................. 7

11.2 Reproducibility ....................................................................................................................................................................................... 7

12 Test report ................................................................................................................................................................................................................... 7

Annex A (informative) Results of interlaboratory tests.................................................................................................................... 8

Bibliography ................................................................................................................................................................................................................................ 9

© ISO 2017 – All rights reserved iii
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SIST EN ISO 15302:2017
ISO 15302:2017(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www .iso .org/ patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO’s adherence to the

World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following

URL: w w w . i s o .org/ iso/ foreword .html.

This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11,

Animal and vegetable fats and oils.

This third edition cancels and replaces the second edition (ISO 15302:2007), of which it constitutes a

minor revision. The scope of this document has been revised to exclude its application to milk and milk

products and their derivatives.
iv © ISO 2017 – All rights reserved
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SIST EN ISO 15302:2017
INTERNATIONAL STANDARD ISO 15302:2017(E)
Animal and vegetable fats and oils — Determination of
benzo[a]pyrene — Reverse-phase high performance liquid
chromatography method
1 Scope

This document specifies a method for the determination of benzo[a]pyrene in crude or refined edible

oils and fats by reverse-phase high performance liquid chromatography (HPLC) using fluorimetric

detection in the range 0,1 µg/kg to 50 µg/kg.

Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this

document.
2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 661, Animal and vegetable fats and oils — Preparation of test sample
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— IEC Electropedia: available at http:// www.e lectropedia. org/
— ISO Online browsing platform: available at http:// www. iso. org/o bp
3.1
benzo[a]pyrene content

mass fraction of benzo[a]pyrene in the test portion, as determined using the method specified in this

document
Note 1 to entry: The content is expressed in micrograms per kilogram.
4 Principle

A test portion is dissolved in light petroleum and benzo[b]chrysene is added as internal standard. A

suitable amount of sample is adsorbed on an alumina column and eluted with light petroleum to remove

any benzo[a]pyrene present.
5 Reagents

Use only reagents of recognized analytical grade, unless otherwise specified. Where analytical grade

...

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