EN 16086-2:2011
(Main)Soil improvers and growing media - Determination of plant response - Part 2: Petri dish test using cress
Soil improvers and growing media - Determination of plant response - Part 2: Petri dish test using cress
This European Standard describes a method for the routine determination of the effect of soil improvers and growing media or constituents thereof on the germination and early root development of cress.
Bodenverbesserungsmittel und Kultursubstrate - Bestimmung der Pflanzenverträglichkeit - Teil 2: Petrischalentest mit Kresse
Diese Europäische Norm beschreibt ein Verfahren für die Routinebestimmung des Einflusses von Bodenverbesserungsmitteln und Kultursubstraten oder deren Bestandteile auf die Keimung und die frühe Wurzelentwicklung von Kresse.
Amendements du sol et supports de culture - Détermination de la réponse des plantes - Partie 2: Essai en boîte de Pétri avec du cresson
La présente Norme européenne décrit une méthode de détermination en routine de l’effet d’amendements de
sol et de supports de culture ou de leurs constituants sur la germination et le développement précoce des
racines de cresson.
Izboljševalci tal in rastni substrati - Določevanje sprejemljivosti za rastline - 2. del: Preskus v petrijevki s krešo
Ta evropski standard opisuje metodo za rutinsko določevanje učinka izboljševalcev tal in rastnih substratov ali njihovih sestavin na kalitev in zgodnji razvoj korenin kreše.
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Bodenverbesserungsmittel und Kultursubstrate - Bestimmung der Pflanzenverträglichkeit - Teil 2: Petrischalentest mit KresseAmendements au sol et supports de culture - Détermination de la réponse des plantes - Partie 2: Essai en boîte de Petri avec du cressonSoil improvers and growing media - Determination of plant response - Part 2: Petri dish test using cress65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 16086-2:2011SIST EN 16086-2:2012en,fr,de01-marec-2012SIST EN 16086-2:2012SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16086-2
November 2011 ICS 65.080 English Version
Soil improvers and growing media - Determination of plant response - Part 2: Petri dish test using cress
Amendements du sol et supports de culture - Détermination de la réponse des plantes - Partie 2: Essai en boîte de Pétri avec du cresson
Bodenverbesserungsmittel und Kultursubstrate - Bestimmung der Pflanzenverträglichkeit - Teil 2: Petrischalentest mit Kresse This European Standard was approved by CEN on 17 September 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16086-2:2011: ESIST EN 16086-2:2012
Contents Page Foreword .31 Scope .42 Normative references .43 Terms and definitions .44 Principle .55 Choice of methodology .55.1 Contact method .55.2 Extract method .56 Material .56.1 Cress seeds (Lepidium sativum) .56.2 Water of class 3.56.3 Sphagnum peat .56.4 Fertilized and limed Sphagnum peat .66.5 Petri dishes .66.6 Perlite .66.7 Testing facility .66.8 Sieve with 10 mm mesh size .66.9 Filter paper .66.10 Ground limestone .67 Contact method .67.1 General preparation .67.2 Sample storage and preparation .67.3 Procedure .77.4 Evaluation parameters .88 Extract method . 108.1 Preparation of the sample extract . 108.2 Procedure . 108.3 Evaluation parameters . 119 Test report . 11Annex A (informative)
Validation . 12Annex B (normative)
Fist test, nutrient solution . 14B.1 Fist test . 14B.2 Composition of the nutrient solution. 14Bibliography . 16 SIST EN 16086-2:2012
Foreword This document has been prepared by Technical Committee CEN/TC
“Soil improvers and growing media”, the secretariat of which is held by ASI. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2012, and conflicting national standards shall be withdrawn at the latest by May 2012. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. SAFETY PRECAUTIONS – Care should be taken when handling samples that may contain sharps or is of a dusty nature.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. SIST EN 16086-2:2012
2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 13037, Soil improvers and growing media – Determination of pH
EN 13038, Soil improvers and growing media – Determination of electrical conductivity
EN 13040, Soil improvers and growing media – Sample preparation for chemical and physical tests, determination of dry matter content, moisture content and laboratory compacted bulk density EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 plant response variation in cress seed germination and/or growth when sown and grown in a growing medium, soil improver or constituent thereof NOTE Factors causing negative plant growth cannot be identified nor sufficiently quantified by applying this method. 3.2 germination for this method, the seed is said to have germinated as soon as the radicle has emerged from the seed 3.3 root length index percentage difference of the root length of germinated cress seeds on the material under investigation compared to the root length of the control 3.4 Munoo-Liisa Vitality index index calculated from the germination rate and the root length SIST EN 16086-2:2012
4 Principle Cress seeds are exposed to the test material for a few days under controlled conditions. The germination and growth of young roots are measured and compared with a control sample. The inhibition of germination and growth of young roots may be caused by phytotoxic substances. If the electrical conductivity (EC) in the diluted material is greater than 80 mS m-1 according to EN 13038, the sample is diluted with sphagnum peat. In this case, the test does not measure the adverse effect of high EC of materials on germination and root development. NOTE 1 In the case of composted materials, these phytotoxic substances can be for instance ammonia, ethylene oxide or short chain fatty acids.
NOTE 2 The test can also be used as an indication of the instability and “immaturity” of the material. If required (for example to fulfil certain quality certification requirements or legislation), materials can be tested without checking the EC. For testing of specific effects, the use of additional plant species such as Chinese cabbage or lettuce may be considered. 5 Choice of methodology 5.1 Contact method For most of growing media or soil improvers, the petri dish test can be carried out with the seeds in physical contact with the material to be tested. 5.2 Extract method Coarse samples such as bark, expanded clay, lava, mineral wool, perlite, polyurethane and pumice, used at 100 % as a growing medium, are not suitable for this procedure. For these materials, the seeds should be in contact with a filter paper thoroughly wetted with an extract of the material to be tested. 6 Material 6.1 Cress seeds (Lepidium sativum) Germination capacity ≥ 95 %. 6.2 Water of class 3 According to EN ISO 3696. 6.3 Sphagnum peat
Sphagnum peat with a degree of humification H3 – H5 according to von Post scale, a pH between 3,0 and 4,5 (measured according to EN 13037), an EC of between 1 and 5 mS m-1 (measured according to EN 13038) and a particle size < 10 mm; without pH-adjustment or fertilizer addition. SIST EN 16086-2:2012
Sphagnum peat (see 6.3), pH-adjusted using ground limestone to a range between 5,5 and 6,5 measured according to EN 13037, fertilized with a water soluble complete fertilizer with essential micronutrients, supplying (225 ± 25) mg N · l-1 (for example 1,5 g · l-1 water soluble complete fertilizer
N : P2O5 : K2O - 15 : 10 : 20). NOTE See Annex B.2. 6.5 Petri dishes
Square, nominal 100 mm length and width, nominal 18 mm height. 6.6 Perlite Particle size < 2,5 mm, maximum 20 % W/W < 0,5 mm. 6.7 Testing facility
Temperature controlled room or growth chamber which can be set at (25 ± 5) °C. 6.8 Sieve with 10 mm mesh size 6.9 Filter paper Approximately 1,42 mm thickness, approximately 700g/m2 weight (for example Whatman “blotter light blue 3644” or equivalent product). 6.10 Ground limestone Finely ground limestone, containing at least 5 % MgCO3, having a particle size less than 1 mm and a moisture content of less than 1 % m/m.
7 Contact method 7.1 General preparation Pass the sample through a 10 mm sieve (see 6.8). Any foreign material such as plastic, metal or glass retained on the sieve shall be removed, the percentage shall be noted. Any other material retained on the sieve which is an intrinsic part of the sample shall be physically reduced to parts of similar size as few times as are necessary to permit the entire sample to pass through the sieve. Fibrous materials i.e. coir fibres and straw shall be cut to a length ≤ 10 mm by using scissors. Thoroughly mix the laboratory sample with the broken particles retained on the sieve taking care to minimise physical damage to the sample as a whole. Transportation and possible storage of the samples shall be done in accordance with EN 13040, using food grade polyethylene bags. NOTE In cases where the proportion of the retained material is above 30 % weight, the extract method is more appropriate. 7.2 Sample storage and preparation If necessary, samples can be stored according to EN 13040. The material to be tested shall be moistened to the approximate optimum moisture content according to the fist test (see Annex B).
The electrical conduc
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