EN ISO 12228-2:2014

Determination of individual and total sterols contents - Gas chromatographic method - Part 2: Olive and olive pomace oils (ISO 12228-2:2014)

ISO 12228-2:2014 specifies a procedure for the gas chromatographic determination of the contents and composition of sterols and triterpene dialcohols in olive and olive pomace oils. For the determination of the contents and composition of sterols in all other animal and vegetable fats and oils, ISO 12228‑1 is to be used.

Bestimmung der individuellen und der Gesamtsterine - Gaschromatographisches Verfahren - Teil 2: Oliven- und Oliventresteröle (ISO 12228-2:2014)

Diese Internationale Norm legt ein Verfahren zur gaschromatographischen Bestimmung der Zusammen-setzung und des Gehalts an Sterinen und Triterpen-Dialkoholen in Oliven  und Oliventresterölen fest. Für die Bestimmung der Gehalte und der Zusammensetzung der Sterine in allen anderen tierischen und pflanzlichen Fetten und Ölen muss der Teil 1 dieser Norm angewendet werden.
ANMERKUNG   Diese Internationale Norm ist technisch identisch mit dem IOC Standard COI/T.20/Doc. No. 30 (November 2011).

Détermination de la teneur en stérols individuels et totaux - Méthode par chromatographie en phase gazeuse - Partie 2: Huile d'olive et huile de grignons d'olive (ISO 12228-2:2014)

L'ISO 12228-2:2014 spécifie un mode opératoire de détermination de la teneur et de la composition des stérols et des dialcools triterpéniques contenus dans l'huile d'olive et l'huile de grignons d'olive par chromatographie en phase gazeuse. Pour déterminer la teneur et la composition des stérols dans tout autre corps gras d'origine animale ou végétale, l'ISO 12228-1 doit être utilisée.

Določevanje posameznih in celotnih sterolov - Plinska kromatografska metoda - 2. del: Oljčna olja in olja iz oljčnih tropin (ISO 12228-2:2014)

Ta del standarda ISO 12228 določa postopek za plinsko kromatografsko določevanje vsebnosti
in sestave sterolov in triterpen dialkoholov v oljčnih oljih in oljih iz oljčnih tropin. Za določevanje vsebnosti in sestave sterolov v vseh drugih živalskih in rastlinskih masteh in oljih se uporablja standard ISO 12228-1.
OPOMBA: Ta del standarda ISO 12228 je tehnično identičen kot standard IOC COI/T.20/št. dok. 30 (november 2011).

General Information

• Status: Published
• Publication Date: 14-Oct-2014
• Withdrawal Date: 29-Apr-2015
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Oct-2014
• Completion Date: 15-Oct-2014
• Ref Project: SIST EN ISO 12228-2:2015 - Determination of individual and total sterols contents - Gas chromatographic method - Part 2: Olive and olive pomace oils (ISO 12228:2014)

Relations

• Replaces: EN ISO 12228:1999 - Animal and vegetable fats and oils - Determination of individual and total sterols contents - Gas chromatographic method (ISO 12228:1999)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2015
1DGRPHãþD
SIST EN ISO 12228:2000
'RORþHYDQMHSRVDPH]QLKLQFHORWQLKVWHURORY3OLQVNDNURPDWRJUDIVNDPHWRGD
GHO2OMþQDROMDLQROMDL]ROMþQLKWURSLQ,62
Determination of individual and total sterols contents - Gas chromatographic method -
Part 2: Olive and olive pomace oils (ISO 12228:2014)
Bestimmung der individuellen und der Gesamtsterine - Gaschromatographisches
Verfahren - Teil 2: Oliven- und Oliventresteröle (ISO 12228:2014)
Détermination de la teneur en stérols individuels et totaux - Méthode par
chromatographie en phase gazeuse - Partie 2: Huile d'olive et huile de grignons d'olive
(ISO 12228:2014)
Ta slovenski standard je istoveten z: EN ISO 12228-2:2014
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 12228-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2014
ICS 67.200.10 Supersedes EN ISO 12228:1999
English Version
Determination of individual and total sterols contents - Gas
chromatographic method - Part 2: Olive and olive pomace oils
(ISO 12228:2014)
Détermination de la teneur en stérols individuels et totaux - Bestimmung der individuellen und der Gesamtsterine -
Méthode par chromatographie en phase gazeuse - Partie 2: Gaschromatographisches Verfahren - Teil 2: Oliven- und
Huile d'olive et huile de grignons d'olive (ISO 12228-2:2014) Oliventresteröle (ISO 12228-2:2014)
This European Standard was approved by CEN on 6 September 2014.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12228-2:2014 E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (EN ISO 12228-2:2014) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and
oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by April 2015, and conflicting national standards shall be withdrawn at the
latest by April 2015.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 12228:1999.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 12228-2:2014 has been approved by CEN as EN ISO 12228-2:2014 without any modification.
INTERNATIONAL ISO
STANDARD 12228-2
First edition
2014-10-01
Determination of individual
and total sterols contents — Gas
chromatographic method —
Part 2:
Olive oils and olive pomace oils
Détermination de la teneur en stérols individuels et totaux —
Méthode par chromatographie en phase gazeuse —
Partie 2: Huile d’olive et huile de grignons d’olive
Reference number
ISO 12228-2:2014(E)
©
ISO 2014
ISO 12228-2:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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Tel. + 41 22 749 01 11
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Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO 12228-2:2014(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 3
7 Sample . 4
7.1 Sampling . 4
7.2 Preparation of the test sample . 4
8 Procedure. 4
8.1 Test portion . 4
8.2 Preparation of unsaponifiable matter. 4
8.3 Separation of the sterol and triterpene dialcohols (erythrodiol, uvaol) fractions by TLC . 5
8.4 Preparation of the trimethylsilyl ethers . 6
8.5 Gas chromatographic analysis . 6
9 Expression of results . 7
9.1 Quantitative evaluation . 7
9.2 Determination of the total sterol content . 8
9.3 Composition of sterols . 8
9.4 Composition of triterpene dialcohols . 8
10 Precision . 9
10.1 Interlaboratory test. 9
10.2 Repeatability limit, r . 9
10.3 Reproducibility limit, R . 9
11 Test report . 9
Annex A (informative) Figures .10
Annex B (informative) Interlaborative trial .13
Bibliography .16
ISO 12228-2:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
This first edition of ISO 12228-2 cancels and replaces ISO 12228:1999, which has been technically
revised.
ISO 12228 consists of the following parts, under the general title Determination of individual and total
sterols contents — Gas chromatographic method:
— Part 1: Animal and vegetable fats and oils
— Part 2: Olive oils and olive pomace oils
iv © ISO 2014 – All rights reserved

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