Animal and vegetable fats and oils - Separation of lipid classes by capillary gas chromatography (fingerprint method) (ISO/TS 22115:2021)

This document specifies a method for the semi-quantitative analysis of oils, fats and oil/fat-related samples (deodistillates).
It is applicable to the screening of oils, fats and oil/fat-related samples to obtain main (e.g. triglycerides) and minor (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol) component information in one single analysis. For a truly quantitative analysis of pre-identified compound classes, specific methods are more appropriate.
The method can also be used as a useful qualitative screening tool for the relative comparison of sample compositions.

Tierische und pflanzliche Fette und Öle - Trennung von Lipidklassen mittels Kapillargaschromatographie (Fingerprint-Verfahren) (ISO/TS 22115:2021)

Dieses Dokument legt ein Verfahren für die semiquantitative Analyse von Ölen, Fetten und öl-/fettbezogenen Proben (Deodestillaten) fest.
Es ist anwendbar für das Screening von Ölen, Fetten und öl-/fettbezogenen Proben, um Informationen über die Hauptbestandteile (z. B. Triglyceride) und Nebenbestandteile (z. B. Sterole, Sterolester, Tocopherole, Wachsester, Fettalkohole und Glycerol) in einer einzigen Analyse zu erhalten. Für eine wirklich quantitative Analyse von vorab identifizierten Verbindungsklassen sind spezifische Verfahren zweckmäßiger.
Das Verfahren kann auch als ein nützliches qualitatives Screening-Werkzeug für den relativen Vergleich von Probenzusammensetzungen angewendet werden.

Corps gras d'origines animale et végétale - Séparation des classes lipidiques par chromatographie en phase gazeuse sur colonne capillaire (méthode fingerprint) (ISO/TS 22115:2021)

Le présent document spécifie une méthode d’analyse semi-quantitative des corps gras et échantillons dérivés de corps gras (déodistillats).
Il est applicable à l’analyse des corps gras et échantillons dérivés de corps gras pour obtenir des informations sur les composés majoritaires (par exemple triglycérides) et minoritaires (par exemple stérols, esters de stérol, tocophérols, esters de cire, alcools gras, glycérol) au cours d'une seule analyse. Pour une analyse réellement quantitative des classes de composés préalablement identifiées, les méthodes spécifiques sont plus appropriées.
La méthode peut également être utilisée comme outil d’analyse qualitative en vue d'effectuer une comparaison relative des compositions d'échantillons.

Živalske in rastlinske maščobe in olja - Ločevanje lipidnih razredov s kapilarno plinsko kromatografijo (metoda fingerprint) (ISO/TS 22115:2021)

General Information

Status
Published
Publication Date
06-Jul-2021
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
07-Jul-2021
Completion Date
07-Jul-2021

Buy Standard

Technical specification
TS CEN ISO/TS 22115:2021
English language
29 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)


SLOVENSKI STANDARD
01-september-2021
Živalske in rastlinske maščobe in olja - Ločevanje lipidnih razredov s kapilarno
plinsko kromatografijo (metoda fingerprint) (ISO/TS 22115:2021)
Animal and vegetable fats and oils - Separation of lipid classes by capillary gas
chromatography (fingerprint method) (ISO/TS 22115:2021)
Tierische und pflanzliche Fette und Öle - Relative Zusammensetzung von Ölen und
Derivaten mittels Kapillargaschromatographie (Fingerprint-Verfahren) (ISO/TS
22115:2021)
Corps gras d'origines animale et végétale - Séparation des classes lipidiques par
chromatographie en phase gazeuse sur colonne capillaire (méthode fingerprint) (ISO/TS
22115:2021)
Ta slovenski standard je istoveten z: CEN ISO/TS 22115:2021
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

CEN ISO/TS 22115
TECHNICAL SPECIFICATION
SPÉCIFICATION TECHNIQUE
July 2021
TECHNISCHE SPEZIFIKATION
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Separation of lipid
classes by capillary gas chromatography (fingerprint
method) (ISO/TS 22115:2021)
Corps gras d'origines animale et végétale - Séparation Tierische und pflanzliche Fette und Öle - Relative
des classes lipidiques par chromatographie en phase Zusammensetzung von Ölen und Derivaten mittels
gazeuse sur colonne capillaire (méthode fingerprint) Kapillargaschromatographie (Fingerprint-Verfahren)
(ISO/TS 22115:2021) (ISO/TS 22115:2021)
This Technical Specification (CEN/TS) was approved by CEN on 29 January 2021 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN ISO/TS 22115:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (CEN ISO/TS 22115:2021) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN websites.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO/TS 22115:2021 has been approved by CEN as CEN ISO/TS 22115:2021 without any
modification.
TECHNICAL ISO/TS
SPECIFICATION 22115
First edition
2021-06
Animal and vegetable fats and oils —
Separation of lipid classes by capillary
gas chromatography (fingerprint
method)
Corps gras d'origines animale et végétale — Séparation des classes
lipidiques par chromatographie en phase gazeuse sur colonne
capillaire (méthode fingerprint)
Reference number
ISO/TS 22115:2021(E)
©
ISO 2021
ISO/TS 22115:2021(E)
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved

ISO/TS 22115:2021(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sample . 3
7.1 Sampling . 3
7.2 Preparation of test sample . 3
8 Procedure. 3
8.1 Preparation of the internal standard, tridecanoylglycerol (5.2.5), c = 20 mg/ml . 3
8.2 Preparation of the individual standard solutions for determination of response factors . 3
8.3 Silylation of the standards . 4
8.4 Preparation of the sample . 4
8.5 Sample solution . 4
8.6 Silylation of the sample . 4
8.7 Gas chromatography . 4
8.8 Peak identification and integration . 5
9 Result of the determination . 6
9.1 Calculation of the response factor . 6
9.2 Quantitative determination . 6
10 Precision of the method . 7
10.1 Interlaboratory test. 7
10.2 Repeatability . 7
10.3 Reproducibility . 7
11 Test report . 7
Annex A (informative) Typical chromatograms . 8
Annex B (informative) Results of an interlaboratory test .18
Bibliography .22
ISO/TS 22115:2021(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11,
Animal and vegetable fats and oils, in collaboration with the European Committee for Standardization
(CEN) Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their by-
products — Methods of sampling and analysis, in accordance with th
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.