EN ISO 21285:2020

Soil quality - Inhibition of reproduction of the soil mite (Hypoaspis aculeifer) by soil contaminants (ISO 21285:2019)

This document specifies a chronic test method for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Hypoaspis aculeifer by ? mainly ? alimentary uptake. This method is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites under concern and waste materials (e.g. dredged material, municipal sludge from a wastewater treatment plant, composed material, or manure, especially those for possible land disposal). The reproduction (= number of juveniles) is the measured parameter of the test. The test reflects the bioavailability of a mixture of contaminants in natural soils (contaminated site soils) to a species which represents a trophic level which is not covered by other ISO standards. This test is not intended to replace the earthworm (see ISO 11268-2) or Collembola (see ISO 11267) reproduction tests since this species belongs not only to a different trophic group but also a different taxonomic group (= mites; i.e. arachnids) than those used usually.
Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. Depending on the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil).
This document provides information on how to use this method for testing samples (soils or substances) under temperate conditions.
This document is not applicable to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa at 25 °C.
NOTE       The stability of the test substance cannot be ensured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.

Bodenbeschaffenheit - Hemmung der Reproduktion von Raubmilben (Hypoaspis aculeifer) durch Bodenverunreinigungen (ISO 21285:2019)

Dieses Dokument legt ein chronisches Verfahren zur Bewertung der Lebensraumfunktion von Böden und zur Bestimmung der Wirkungen von Bodenverunreinigungen und Substanzen auf die Reproduktion von Hypoaspis aculeifer — hauptsächlich — bei Aufnahme durch die Nahrung fest. Diese Methode ist anwendbar für Böden und Bodenmaterialien unbekannter Beschaffenheit, z. B. von verunreinigten Standorten, beauf¬schlagten Böden, Böden nach der Sanierung, industriellen, landwirtschaftlichen oder anderen betroffenen Standorten und Abfallmaterialien (z. B. Baggergut, kommunaler Schlamm aus einer Kläranlage, Kompost¬material oder Stalldung, speziell solche für eine mögliche Landdeponie). Die Reproduktion (= Anzahl der Juvenilen) ist der gemessene Parameter des Tests. Die Prüfung gibt die Bioverfügbarkeit einer Mischung von Kontaminanten in natürlichen Böden (Böden von verunreinigten Standorten) für eine Art wieder, die eine trophische Ebene repräsentiert, die nicht von anderen ISO Standards abgedeckt ist. Diese Prüfung soll nicht die Reproduktionsprüfung von Regenwürmern (ISO 11268 2) oder Collembolen (ISO 11267) ersetzen, da diese Spezies nicht nur zu einer anderen trophischen Gruppe, sondern auch zu einer anderen taxonomischen Gruppe (= Milben; d. h. Arachniden) als die üblicherweise verwendeten gehört.
Die Wirkungen der Substanzen werden mit einem Standardboden, vorzugsweise mit einem festgelegten künstlichen Bodensubstrat, bewertet. Bei verunreinigten Böden werden die Wirkungen im zu prüfenden Boden und in einem Kontrollboden bestimmt. Abhängig von der Zielsetzung der Untersuchung sind die Kontrolle und das zur Verdünnung verwendete Substrat (Verdünnungsreihen des verunreinigten Bodens) entweder ein mit dem zu prüfenden Boden vergleichbarer, nicht verunreinigter Boden (Referenzboden) oder ein Standardboden (z. B. künstlicher Boden).
Dieses Dokument gibt Informationen, wie dieses Verfahren zur Prüfung von Proben (Böden oder Substanzen) unter gemäßigten Bedingungen anzuwenden ist.
Dieses Dokument gilt nicht für Substanzen, deren Luft /Boden Verteilungskoeffizient größer eins ist oder für Substanzen mit einem Dampfdruck von über 300 Pa bei 25 °C.
ANMERKUNG Es kann nicht sichergestellt werden, dass die Prüfsubstanz während der gesamten Versuchsdauer stabil bleibt. Im Prüfverfahren wird die Persistenz der Prüfsubstanz nicht überwacht.

Qualité du sol - Inhibition de la reproduction de l'acarien prédateur (Hypoaspis aculeifer) par des contaminants du sol (ISO 21285:2019)

Le présent document décrit une méthode d'essai chronique pour évaluer la fonction d'habitat des sols et déterminer les effets des contaminants du sol et des substances sur la reproduction de l'espèce Hypoaspis aculeifer par — principalement — absorption par voie alimentaire. Cette méthode est applicable aux sols et matériaux du sol de qualité inconnue, par exemple, provenant de sites contaminés, de sols amendés, de sols ayant fait l'objet d'une remédiation, de sites industriels, agricoles et autres, et aux déchets (par exemple, matériau de dragage, boue résiduaire des stations d'épuration des eaux usées, engrais ou fumier, notamment ceux pour épandage éventuel). La reproduction (= nombre de juvéniles) est le paramètre mesuré au cours de l'essai. L'essai reflète la biodisponibilité d'un mélange de contaminants dans les sols naturels (sols de sites contaminés) vis-à-vis d'une espèce qui représente un niveau trophique qui n'est pas couvert par les autres normes ISO. Il n'est pas prévu d'utiliser cet essai pour remplacer les essais de reproduction vis-à-vis des vers de terre (voir ISO 11268-2) ou des collemboles (voir ISO 11267) car cette espèce appartient non seulement à un autre groupe trophique, mais également à un autre groupe taxonomique (= acariens; c'est-à-dire, arachnides) que les autres espèces utilisées habituellement.
Les effets des substances sont évalués en utilisant un sol standard, de préférence un substrat défini de sol artificiel. Pour les sols contaminés, les effets sont déterminés dans le sol à analyser et dans un sol témoin. Selon l'objectif de l'étude, le substrat témoin et le substrat de dilution (séries de dilutions du sol contaminé) sont soit un sol non contaminé comparable au sol à analyser (sol de référence), soit un sol standard (par exemple, sol artificiel).
Le présent document fournit des informations sur la façon d'utiliser cette méthode pour analyser des échantillons (sols ou substances) dans des conditions tempérées.
Il n'est pas applicable aux substances pour lesquelles le coefficient de partage air/sol est supérieur à 1, ou aux substances dont la tension de vapeur dépasse 300 Pa à 25 °C.
NOTE       La stabilité de la substance d'essai ne peut pas être assurée tout au long de la période d'essai. La méthode d'essai ne prévoit pas de contrôler la persistance de la substance soumise à essai.

Kakovost tal - Zaviranje razmnoževanja pršice (Hypoaspis aculeifer) zaradi onesnaževal v tleh (ISO 21285:2019)

General Information

• Status: Published
• Publication Date: 28-Apr-2020
• Withdrawal Date: 30-Oct-2020
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444 - Environmental characterization
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 29-Apr-2020
• Due Date: 07-Mar-2022
• Completion Date: 29-Apr-2020
• Ref Project: SIST EN ISO 21285:2020 - Soil quality - Inhibition of reproduction of the soil mite (Hypoaspis aculeifer) by soil contaminants (ISO 21285:2019)

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2020
Kakovost tal - Zaviranje razmnoževanja pršice (Hypoaspis aculeifer) zaradi
onesnaževal v tleh (ISO 21285:2019)
Soil quality - Inhibition of reproduction of the soil mite (Hypoaspis aculeifer) by soil
contaminants (ISO 21285:2019)
Bodenbeschaffenheit - Hemmung der Reproduktion von Raubmilben (Hypoaspis
aculeifer) durch Bodenverunreinigungen (ISO 21285:2019)
Qualité du sol - Inhibition de la reproduction de l'acarien prédateur (Hypoaspis aculeifer)
par des contaminants du sol (ISO 21285:2019)
Ta slovenski standard je istoveten z: EN ISO 21285:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 21285
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Inhibition of reproduction of the soil mite
(Hypoaspis aculeifer) by soil contaminants (ISO
21285:2019)
Qualité du sol - Inhibition de la reproduction de Bodenbeschaffenheit - Hemmung der Reproduktion
l'acarien prédateur (Hypoaspis aculeifer) par des von Raubmilben (Hypoaspis aculeifer) durch
contaminants du sol (ISO 21285:2019) Bodenverunreinigungen (ISO 21285:2019)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21285:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 21285:2019 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 21285:2020 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2020, and conflicting national standards shall
be withdrawn at the latest by October 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 21285:2019 has been approved by CEN as EN ISO 21285:2020 without any modification.

INTERNATIONAL ISO
STANDARD 21285
First edition
2019-04
Soil quality — Inhibition of
reproduction of the soil mite
(Hypoaspis aculeifer) by soil
contaminants
Qualité du sol — Inhibition de la reproduction de l'acarien prédateur
(Hypoaspis aculeifer) par des contaminants du sol
Reference number
ISO 21285:2019(E)
©
ISO 2019
ISO 21285:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 21285:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 3
5 Reagents and material . 4
5.1 Biological material . 4
5.2 Test mixtures . 4
5.3 Reference substance . 5
6 Apparatus . 6
7 Procedure. 6
7.1 Experimental design . 6
7.1.1 General. 6
7.1.2 Range-finding test (preliminary test) . 7
7.1.3 Definitive test . 7
7.1.4 Limit test . 7
7.2 Preparation of test mixtures . 8
7.2.1 Testing contaminated soil . 8
7.2.2 Testing substances added to the test substrate . 8
7.2.3 Preparation of control containers . 9
7.3 Addition of the biological material . 9
7.4 Test conditions and measurements . 9
7.5 Feeding of the mites. 9
7.6 Determination of surviving predatory mites .10
8 Calculation and expression of results .10
8.1 Calculation .10
8.2 Expression of results .10
9 Validity of the test .10
10 Statistical analysis .11
10.1 General .11
10.2 Single-concentration tests .11
10.3 Multi-concentration tests.11
10.3.1 Range-finding test .11
10.3.2 Definitive test .11
11 Test report .12
Annex A (informative) Techniques for rearing and breeding of predatory mites .14
Annex B (normative) Determination of water-holding capacity .16
Annex C (informative) Guidance on adjustment of pH of artificial soil .17
Annex D (informative) Extraction and counting of predatory mites .18
Annex E (informative) Basic information on the biology of Hypoaspis (Geolaelaps) aculeifer .19
Bibliography .20
ISO 21285:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the I
...