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EN ISO 20130:2020

(Main)

Soil quality - Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates (ISO 20130:2018)

Soil quality - Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates (ISO 20130:2018)

This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.

Bodenbeschaffenheit - Messung von Enzymaktivitätsmustern in Bodenproben mit kolorimetrischen Substraten in Mikrotiterplatten (ISO 20130:2018)

Dieses Dokument legt ein Verfahren zur gemeinsamen (oder getrennten) Messung verschiedener Hydrolase-Aktivitäten (Arylamidase, Arylsulfatase, β Galactosidase, α Glucosidase, β Glucosidase, N Acetyl-Glucosa¬minidase, saure, alkalische und globale Phosphatasen, Urease) in Bodenproben unter Verwendung von kolorimetrischen Substraten fest. Die Enzymaktivitäten im Boden variieren je nach Jahreszeit und sind abhängig von chemischen, physikalischen und biologischen Eigenschaften des Bodens.
Diese Methode kann entweder zum Nachweis schädlicher Auswirkungen auf die Enzymaktivitäten im Boden durch toxische Substanzen oder andere anthropogene Stoffe in kontaminierten Böden gegenüber einem Kontrollboden oder zur Prüfung von Chemikalien angewendet werden.

Qualité du sol - Mesure de l'activité enzymatique dans des échantillons de sol en utilisant des substrats colorimétriques (ISO 20130:2018)

Le présent document spécifie une méthode de mesure simultanée (ou non) de plusieurs activités des hydrolases (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acétylglucosaminidase, phosphatases acides, alcalines et globales, uréase) dans des échantillons de sol en utilisant des substrats colorimétriques. Les activités enzymatiques du sol varient en fonction des saisons et dépendent des caractéristiques chimiques, physiques et biologiques du sol. Cette méthode peut être appliquée soit pour la détection des effets nocifs de substances toxiques ou d'autres agents anthropiques dans un sol contaminé par comparaison avec un sol de référence, soit pour la réalisation d'essais sur des produits chimiques.

Kakovost tal - Merjenje encimske aktivnosti v vzorcih tal s kolorometričnimi substrati na mikrotitrskih ploščah (ISO 20130:2018)

General Information

Status
Published
Publication Date
05-May-2020
Withdrawal Date
29-Nov-2020
ICS
13.080.30 - Biological properties of soils
Technical Committee
CEN/TC 444 - Environmental characterization
Drafting Committee
CEN/TC 444 - Environmental characterization
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
06-May-2020
Due Date
07-Mar-2022
Completion Date
06-May-2020
Ref Project
SIST EN ISO 20130:2020 - Soil quality - Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates (ISO 20130:2018)

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 20130:2020
01-november-2020
Kakovost tal - Merjenje encimske aktivnosti v vzorcih tal s kolorometričnimi
substrati na mikrotitrskih ploščah (ISO 20130:2018)
Soil quality - Measurement of enzyme activity patterns in soil samples using colorimetric
substrates in micro-well plates (ISO 20130:2018)
Bodenbeschaffenheit - Messung von Enzymaktivitätsmustern in Bodenproben mit
kolorimetrischen Substraten in Mikrotiterplatten (ISO 20130:2018)
Qualité du sol - Mesure de l'activité enzymatique dans des échantillons de sol en utilisant
des substrats colorimétriques (ISO 20130:2018)
Ta slovenski standard je istoveten z: EN ISO 20130:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 20130:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 20130:2020

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SIST EN ISO 20130:2020


EN ISO 20130
EUROPEAN STANDARD

NORME EUROPÉENNE

May 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version

Soil quality - Measurement of enzyme activity patterns in
soil samples using colorimetric substrates in micro-well
plates (ISO 20130:2018)
Qualité du sol - Mesure de l'activité enzymatique dans Bodenbeschaffenheit - Messung von
des échantillons de sol en utilisant des substrats Enzymaktivitätsmustern in Bodenproben mit
colorimétriques (ISO 20130:2018) kolorimetrischen Substraten in Mikrotiterplatten (ISO
20130:2018)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20130:2020 E
worldwide for CEN national Members.

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SIST EN ISO 20130:2020
EN ISO 20130:2020 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 20130:2020
EN ISO 20130:2020 (E)
European foreword
The text of ISO 20130:2018 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 20130:2020 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2020, and conflicting national standards
shall be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 20130:2018 has been approved by CEN as EN ISO 20130:2020 without any modification.

3

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SIST EN ISO 20130:2020

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