prEN 49-2
(Main)Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method)
Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method)
This document specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has been treated previously by full impregnation. This method is applicable to:
- water-insoluble chemicals which are being studied as active insecticides;
- organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
- organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates;
- water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven - Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Dieses Dokument legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens der Larven in einem zuvor durch Volltränkung behandelten Holz fest. Das Verfahren ist anwendbar für:
wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
organische Formulierungen in ihrem Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
organische in Wasser
dispergierbare Formulierungen in ihrem Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
wasserlösliche Stoffe, z. B. Salze.
Das Verfahren ist anwendbar, unabhängig davon, ob die Prüfkörper einer angemessenen Alterungsbeanspruchungen ausgesetzt wurden oder nicht.
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des larves - Partie 2 : Application par imprégnation (Méthode de laboratoire)
Le présent document spécifie une méthode de détermination de l'efficacité protectrice ou du seuil d'efficacité d'un produit de préservation du bois vis-à-vis d'Anobiurn punctatum (De Geer) par observation de la ponte et de la survie des larves dans du bois préalablement traité par imprégnation totale. Cette méthode s'applique :
— aux produits chimiques non hydrosolubles étudiés en tant que matières actives ;
— aux formulations organiques telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ;
— aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ; et
— aux produits hydrosolubles, par exemple des sels.
La méthode est applicable que les éprouvettes aient été ou non soumises à des procédures de vieillissement appropriées.
Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število odloženih jajčec in preživelih ličink - 2. del: Postopek z globinsko impregnacijo lesa (laboratorijska metoda)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2025
Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite
proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število
odloženih jajčec in preživelih ličink - 2. del: Postopek z globinsko impregnacijo
lesa (laboratorijska metoda)
Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis
d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des
larves - Partie 2 : Application par imprégnation (Méthode de laboratoire)
Ta slovenski standard je istoveten z: prEN 49-2
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
DRAFT
EUROPEAN STANDARD
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 71.100.50 Will supersede EN 49-2:2015
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by
egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'efficacité protectrice vis à vis de Anobium punctatum Wirkung gegenüber Anobium punctatum (De Geer)
(De Geer) par l'observation de la ponte et de la survie durch Beobachten der Eiablage und des Überlebens
des larves - Partie 2 : Application par imprégnation von Larven - Teil 2: Anwendung durch Volltränkung
(Méthode de laboratoire) (Laboratoriumsverfahren)
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 49-2:2025 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative reference . 5
3 Terms and definitions . 5
4 Principle . 5
5 Test materials . 6
5.1 Biological material . 6
5.2 Products and reagents . 6
5.3 Apparatus . 7
6 Sampling . 8
7 Test specimens . 8
7.1 Species of wood . 8
7.2 Wood quality . 8
7.3 Provision of test specimens . 8
7.4 Dimensions of test specimens . 8
7.5 Number of test specimens . 9
8 Procedure. 9
8.1 Preparation of the test specimens . 9
8.1.1 Conditioning of test specimens before treatment . 9
8.1.2 Treatment of the test specimens . 9
8.1.3 Drying and conditioning of the test specimens after treatment . 10
8.2 Exposure of the test specimens to the insects . 11
8.3 Conditions and duration of the test . 11
8.4 Examination of the test specimens . 11
9 Validity of test . 12
10 Expression of results . 12
10.1 Assessment of the protective effectiveness . 12
10.2 Toxic values . 12
11 Test report . 12
Annex A (informative) Example of a test report . 14
Annex B (informative) Identification of sex of test insects (Anobium punctatum). 16
Annex C (informative) Culturing technique for Anobium punctatum. 17
Annex D (informative) Environmental, health and safety precautions within
chemical/biological laboratory . 20
Bibliography . 21
European foreword
This document (prEN 49-2:2025) has been prepared by Technical Committee CEN/TC 38 “Biological
durability of wood and wood-based products”, the secretariat of which is held by SIS.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 49-2:2015.
Significant technical differences between this document and EN 49-2:2015 are as follows:
a) requirements for temperature and humidity were aligned with other insect test standards (5.3.1;
5.3.5);
b) imaging facilities for non-destructive recordings of insect tunnels inside test specimens were
generalized (5.3.13);
c) storage limitations for wood from which test specimens are to be made from have been cancelled
(7.2).
NOTE Test results obtained according to earlier versions of this document or when the tests had started
before this version of EN 49-1 was published are considered as valid.
EN 49, Wood preservatives ― Determination of the protective effectiveness against Anobium punctatum
(De Geer) by egg-laying and larval survival, consists of two parts:
— Part 1: Application by surface treatment (Laboratory method);
— Part 2: Application by impregnation (Laboratory method).
EN 49-1 is required to enable effectiveness assessments of wood preservatives which are intended to
be applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation.
Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the
concentration at which the product prevents the development of infestation from egg laying.
The method simulates conditions which can occur in practice on timber which has been treated some
time previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum
are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In
making this assessment the methods by which the preservative may be applied should be taken into
account. It is further recommended that results from this test should be supplemented by those from
other appropriate tests, and above all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take
suitable precautions to isolate and separate, as far as possible, operations involving chemical products,
other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include
the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special
training for personnel (see also Annex D for environmental, health and safety precautions).
1 Scope
This document specifies a method for the determination of the protective effectiveness or the toxic
values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in
wood which has been treated previously by full impregnation. This method is applicable to:
— water-insoluble chemicals which are being studied as active insecticides;
— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates;
— water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing
procedures.
2 Normative reference
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
EN 73, Durability of wood and wood-based products — Accelerated ageing of treated wood prior to
biological testing — Evaporative ageing procedure
EN 84, Durability of wood and wood-based products — Accelerated ageing of treated wood prior to
biological testing — Leaching procedure
EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average
characteristics of the total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs
laid, the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in
untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of
a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or
diluent treated control test specimens.
Depending on the test being carried out either
— on a set of test specimens of a susceptible wood species that is impregnated with a solution of the
preservative, or
— if toxic values are to be determined, on several sets of test specimens of a susceptible wood species
that are impregnated with a series of solutions in which the concentration of preservative is ranged
in a given progression.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.
Adults to be used in the test shall be collected daily from naturally infested wood or laboratory culture
(see Annex C).
Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.2.2
and C.6) and then checked to ensure that they are undamaged, active, and free from any infestation by
mites. Determine the sex (see Annex B) of the collected and checked adults and place the males and
females in separate containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the end sections of test specimens.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Paste, for securing fine cloth (5.2.7), the paste shall be starch-free, non-toxic to Anobium
punctatum and insoluble in the product under test.
NOTE Sodium carboxymethyl cellulose, food grade, has been found to be suitable.
5.2.3 Xylene, technical grade.
Other products may be used which have been shown suitable for preventing the growth of mould but
not interfere with the test method.
5.2.4 Water, complying with grade 3 of EN ISO 3696 shall be used.
5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not
leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect
on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.
5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation
of the egg-laying zones.
NOTE Cotton, linen or polyamide-gauze have also been proven suitable.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity
(75 ± 5) %.
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity
(65 ± 5) %.
The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided
that this has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Treatment vessel(s), of a material that does not react with the preservative under test, for
example of glass for organic products and of polyethylene for salts containing fluorine.
5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic
materials. Avoid excessive exposure of operators to solvents or their vapours.
5.3.5 Testing chamber, ventilated and air-conditioned, controlled at (22 ± 2) °C and at a relative
humidity of (75 ± 5) %.
5.3.6 Drying vessel(s), capable of holding sets of five test specimens (7.5), provided with a close-
fitting cover and containing supports that will give minimum contact with treated test specimens to be
placed on them. The vessels and supports shall be of a material that does not react with the
preservative under test, for example glass for organic compounds and polyethylene for products
containing fluorine.
5.3.7 Vacuum vessel(s), fitted with stopcocks, capable of receiving the treatment vessels (5.3.3).
)
5.3.8 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa .
5.3.9 Weights, to provide ballast for the test specimens. The weights shall not react with any
materials with which they come into contact during the test.
5.3.10 Safety equipment, protective clothing, protective gloves appropriate for the test product
and the test solvent, to ensure the safety of the operator.
5.3.11 Test containers, suitable for holding the test specimens and of material resistant to the
solvents used, and fitted with perforated covers to provide a good exchange of air.
NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.
5.3.12 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of
0,01 g.
5.3.13 Imaging facilities e.g. X-ray apparatus, or any other device that offers good images (2D), of
internal tunnels caused by small larvae.
1)
100 Pa = 1 mbar.
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored
and handled in accordance with any written recommendations from the supplier.
For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The reference species is European oak. This shall be either sessile oak (Quercus petraea (Mattuschka)
Lieblin) or pedunculate oak (Quercus robur Linnaeus).
)
Additional tests may be carried out using other timber species but, if so, this should be stated in the
test report.
7.2 Wood quality
The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood
shall not have been water-stored, floated, chemically treated or steamed. The wood shall originate from
trees preferably felled in winter. The trees shall be cut immediately after felling and the timber rapidly
air-dried or kiln dried at temperatures below 60 °C.
)
The wood shall be exclusively sapwood and having between two and ten annual rings per 10 mm.
It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimens
)
Prepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum
of 2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the
direction of the grain. The annual rings shall be parallel to the broad faces (contact angle of less than
5°). Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to
give test specimens (50 ± 0,5) mm long.
The test specimens shall originate from a minimum of three trees or shall be taken at random from a
stock originally of more than 500 test specimens.
7.4 Dimensions of test specimens
The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2)
shall be (50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.
For the purposes of calculating the mass of preservative retained per unit volume of wood (8.1.2.2) the
nominal volume of each test specimen shall be taken as 18,75 cm .
Mark each test specimen so that it can be identified throughout the test.
2)
The growth of young larvae of Anobium punctatum is slow in test specimens from resinous wood. Results from test
specimens in resinous wood should be compared with those obtained from oak test specimens.
3)
It is not essential in this test for the starch content to be high.
4)
These test specimens may be taken from the trunk of the tree or the large branches.
7.5 Number of test specimens
Use:
a) five test specimens (no more than two originating from the same tree unless taken at random from
a stock of more than 500) for each preservative and each concentration;
b) five untreated control test specimens (no more than two originating from the same tree unless
taken at random from a stock of more than 500) for a complete test of any given preservative;
c) five control test specimens (no more than two originating from the same tree unless taken at
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