EN ISO 23753-2:2019

Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2019)

This document specifies a method for determining activity of dehydrogenases in soil, using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)[1]-[5]. As the INT reduction is less sensitive to O2, the method is more robust than the TTC-method described in ISO 23753-1.

Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2: Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019)

Dieses Dokument legt ein Verfahren zur Bestimmung der Aktivität von Dehydrogenasen im Boden mit 2 (4 Iodophenyl) 3 (4 nitrophenyl) 5 phenyltetrazoliumchlorid (INT) [1]–[5] fest. Da die INT Reduktion gegenüber O2 weniger empfindlich ist, ist das Verfahren robuster als das in ISO 23753 1 beschriebene TTC Verfahren.

Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2: Méthode au chlorure de iodotétrazolium (INT) (ISO 23753-2:2019)

Le présent document spécifie une méthode de détermination de l'activité des déshydrogénases dans le sol à l'aide de chlorure de 2-(4-iodophényl)-3-(4-nitrophényl)-5-phényltétrazolium (INT).[1]-[5] Comme la réduction de l'INT est moins sensible à la présence d'O2, cette méthode est plus robuste que la méthode au CTT décrite dans l'ISO 23753‑1.

Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 2. del: Metoda z jodotetrazolijevim kloridom (INT) (ISO 23753-2:2019)

Ta dokument določa metodo za določevanje aktivnosti dehidrogenaz v tleh z uporabo 2-(4-jodofenil)-3-(4-nitrofenil)-5-feniltetrazolijevega klorida (INT)[1]-[5]. Ker je redukcija INT manj občutljiva na O2, je metoda bolj robustna kot metoda TTC, opisana v standardu ISO 23753-1.

General Information

• Status: Published
• Publication Date: 19-Mar-2019
• Withdrawal Date: 29-Sep-2019
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444/WG 4 - Biological characterization
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 20-Mar-2019
• Completion Date: 20-Mar-2019
• Ref Project: SIST EN ISO 23753-2:2019 - Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2019)

Relations

• Replaces: EN ISO 23753-2:2011 - Soil Quality - Determination of dehydrogenase activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2005)
• Effective Date: 25-Nov-2015
• Amended By: EN ISO 23753-2:2019/A1:2020 - Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) - Amendment 1 (ISO 23753-2:2019/Amd 1:2020)
• Effective Date: 13-Nov-2019

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 23753-2:2019
01-julij-2019
Nadomešča:
SIST EN ISO 23753-2:2011
Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 2. del: Metoda z
jodotetrazolijevim kloridom (INT) (ISO 23753-2:2019)
Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using
iodotetrazolium chloride (INT) (ISO 23753-2:2019)
Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2:
Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019)
Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2:
Méthode au chlorure de iodotétrazolium (INT) (ISO 23753-2:2019)
Ta slovenski standard je istoveten z: EN ISO 23753-2:2019
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 23753-2:2019 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN ISO 23753-2:2019

---------------------- Page: 2 ----------------------

SIST EN ISO 23753-2:2019


EN ISO 23753-2
EUROPEAN STANDARD

NORME EUROPÉENNE

March 2019
EUROPÄISCHE NORM
ICS 13.080.30 Supersedes EN ISO 23753-2:2011
English Version

Soil quality - Determination of dehydrogenases activity in
soils - Part 2: Method using iodotetrazolium chloride (INT)
(ISO 23753-2:2019)
Qualité du sol - Détermination de l'activité des Bodenbeschaffenheit - Bestimmung der
déshydrogénases dans les sols - Partie 2: Méthode au Dehydrogenaseaktivität in Böden - Teil 2: Verfahren
chlorure de iodotétrazolium (INT) (ISO 23753-2:2019) mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019)
This European Standard was approved by CEN on 4 February 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23753-2:2019 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN ISO 23753-2:2019
EN ISO 23753-2:2019 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------

SIST EN ISO 23753-2:2019
EN ISO 23753-2:2019 (E)
European foreword
This document (EN ISO 23753-2:2019) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Test methods for environmental
characterization of solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2019, and conflicting national standards
shall be withdrawn at the latest by September 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 23753-2:2011.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 23753-2:2019 has been approved by CEN as
...