Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2019)

This document specifies a method for determining activity of dehydrogenases in soil, using
2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)[1]-[5]. As the INT reduction is
less sensitive to O2, the method is more robust than the TTC-method described in ISO 23753-1.

Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2: Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019)

Dieses Dokument legt ein Verfahren zur Bestimmung der Aktivität von Dehydrogenasen im Boden mit 2 (4 Iodophenyl) 3 (4 nitrophenyl) 5 phenyltetrazoliumchlorid (INT) [1]–[5] fest. Da die INT Reduktion gegenüber O2 weniger empfindlich ist, ist das Verfahren robuster als das in ISO 23753 1 beschriebene TTC Verfahren.

Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2: Méthode au chlorure de iodotétrazolium (INT) (ISO 23753-2:2019)

Le présent document spécifie une méthode de détermination de l'activité des déshydrogénases dans le sol à l'aide de chlorure de 2-(4-iodophényl)-3-(4-nitrophényl)-5-phényltétrazolium (INT).[1]-[5] Comme la réduction de l'INT est moins sensible à la présence d'O2, cette méthode est plus robuste que la méthode au CTT décrite dans l'ISO 23753‑1.

Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 2. del: Metoda z jodotetrazolijevim kloridom (INT) (ISO 23753-2:2019)

Ta dokument določa metodo za določevanje aktivnosti dehidrogenaz v tleh z uporabo 2-(4-jodofenil)-3-(4-nitrofenil)-5-feniltetrazolijevega klorida (INT)[1]-[5]. Ker je redukcija INT manj občutljiva na O2, je metoda bolj robustna kot metoda TTC, opisana v standardu ISO 23753-1.

General Information

Status
Published
Public Enquiry End Date
28-Feb-2018
Publication Date
05-Jun-2019
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
15-Apr-2019
Due Date
20-Jun-2019
Completion Date
06-Jun-2019

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SLOVENSKI STANDARD
SIST EN ISO 23753-2:2019
01-julij-2019
Nadomešča:
SIST EN ISO 23753-2:2011
Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 2. del: Metoda z
jodotetrazolijevim kloridom (INT) (ISO 23753-2:2019)

Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using

iodotetrazolium chloride (INT) (ISO 23753-2:2019)
Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2:
Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019)

Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2:

Méthode au chlorure de iodotétrazolium (INT) (ISO 23753-2:2019)
Ta slovenski standard je istoveten z: EN ISO 23753-2:2019
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 23753-2:2019 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 23753-2:2019
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SIST EN ISO 23753-2:2019
EN ISO 23753-2
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2019
EUROPÄISCHE NORM
ICS 13.080.30 Supersedes EN ISO 23753-2:2011
English Version
Soil quality - Determination of dehydrogenases activity in
soils - Part 2: Method using iodotetrazolium chloride (INT)
(ISO 23753-2:2019)

Qualité du sol - Détermination de l'activité des Bodenbeschaffenheit - Bestimmung der

déshydrogénases dans les sols - Partie 2: Méthode au Dehydrogenaseaktivität in Böden - Teil 2: Verfahren

chlorure de iodotétrazolium (INT) (ISO 23753-2:2019) mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019)

This European Standard was approved by CEN on 4 February 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,

Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23753-2:2019 E

worldwide for CEN national Members.
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SIST EN ISO 23753-2:2019
EN ISO 23753-2:2019 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

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SIST EN ISO 23753-2:2019
EN ISO 23753-2:2019 (E)
European foreword

This document (EN ISO 23753-2:2019) has been prepared by Technical Committee ISO/TC 190 "Soil

quality" in collaboration with Technical Committee CEN/TC 444 “Test methods for environmental

characterization of solid matrices” the secretariat of which is held by NEN.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by September 2019, and conflicting national standards

shall be withdrawn at the latest by September 2019.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN ISO 23753-2:2011.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,

France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,

Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,

Turkey and the United Kingdom.
Endorsement notice

The text of ISO 23753-2:2019 has been approved by CEN as EN ISO 23753-2:2019 without any

modification.
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SIST EN ISO 23753-2:2019
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SIST EN ISO 23753-2:2019
INTERNATIONAL ISO
STANDARD 23753-2
Second edition
2019-02
Soil quality — Determination of
dehydrogenases activity in soils —
Part 2:
Method using iodotetrazolium
chloride (INT)
Qualité du sol — Détermination de l'activité des déshydrogénases
dans les sols —
Partie 2: Méthode au chlorure de iodotétrazolium (INT)
Reference number
ISO 23753-2:2019(E)
ISO 2019
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SIST EN ISO 23753-2:2019
ISO 23753-2:2019(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2019

All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may

be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting

on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address

below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
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SIST EN ISO 23753-2:2019
ISO 23753-2:2019(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

Introduction ..................................................................................................................................................................................................................................v

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 1

5 Limitations .................................................................................................................................................................................................................. 1

6 Reagents and materials ................................................................................................................................................................................. 2

7 Apparatus ..................................................................................................................................................................................................................... 2

8 Procedure..................................................................................................................................................................................................................... 3

8.1 Establishment of standard curve ............................................................................................................................................ 3

8.2 Sampling ....................................................................................................................................................................................................... 3

9 Calculation .................................................................................................................................................................................................................. 4

10 Validity criteria ...................................................................................................................................................................................................... 4

10.1 Standard curve ........................................................................................................................................................................................ 4

10.2 Samples ......................................................................................................................................................................................................... 5

11 Test report ................................................................................................................................................................................................................... 5

Annex A (informative) Results of modified parameters ................................................................................................................... 6

Bibliography ................................................................................................................................................................................................................................ 8

© ISO 2019 – All rights reserved iii
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SIST EN ISO 23753-2:2019
ISO 23753-2:2019(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www .iso .org/patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO's adherence to the

World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso

.org/iso/foreword .html.

This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,

Biological characterization.

This second edition cancels and replaces the first edition (ISO 23753-2:2005), which has been

technically revised. The main changes compared to the previous edition are as follows:

— a new Clause 5 “Limitations” has been added;

— in Clause 6, reagents and their preparation have been updated to new results (e.g. concentration of

Tris buffer of 100 mmol/l at pH 7,6, incubation time between 4 h to 6 h);
— new Tables 1 and 2 have been added;
— Clause 10 "Validity criteria" has been added;
— a new Annex A “Results of modified parameters” has been added;
— references in Clause 2 and the Bibliography have been updated.
A list of all the parts in the ISO 23753 series can be found on the ISO website.

Any feedback or questions on this document should be directed to the user’s national standards body. A

complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved
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SIST EN ISO 23753-2:2019
ISO 23753-2:2019(E)
Introduction
The soil microflora is responsible for the de
...

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