EN ISO 29841:2014/A1:2016

Vegetable fats and oils - Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009/AMD 1:2016)

Pflanzliche Fette und Öle - Bestimmung thermischer Abbauprodukte des Chlorophyll a und a' (Pheophytin a, a' und Pyropheophytin) (ISO 29841:2009/AMD 1:2016)

Corps gras d'origine végétale - Détermination des produits de décomposition des chlorophylles a et a' (phéophytines a, a' et pyrophéophytines) (ISO 29841:2009/AMD 1:2016)

Rastlinske maščobe in olja - Določevanje razgradnih produktov klorofila a in a' (feofitini a, a' in pirofeofitini) - Dopolnilo 1 (ISO 29841:2009/AMD 1:2016)

General Information

• Status: Published
• Publication Date: 23-Feb-2016
• Withdrawal Date: 30-Aug-2016
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 24-Feb-2016
• Completion Date: 24-Feb-2016
• Ref Project: SIST EN ISO 29841:2014/A1:2016 - Vegetable fats and oils - Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009/AMD 1:2016)

Relations

• Amends: EN ISO 29841:2014 - Vegetable fats and oils - Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009)
• Effective Date: 02-Jul-2014

Overview

EN ISO 29841:2014/A1:2016 is a European standard developed by CEN in alignment with ISO 29841:2009/AMD 1:2016. It specifies the methods for determining the degradation products of chlorophylls a and a'-specifically pheophytins a, a', and pyropheophytins-in vegetable fats and oils. This standard is crucial for assessing the quality and stability of vegetable oils, focusing on the thermal degradation of chlorophyll compounds, which are natural pigments impacting oil color and quality.

Key Topics

• Scope of Analysis
  The standard addresses the analytical determination of chlorophyll degradation products in vegetable fats and oils, which are vital indicators for the freshness and thermal treatment history of oils.

• Degradation Products Covered

  • Pheophytin a
  • Pheophytin a’
  • Pyropheophytin

• Analytical Methods

  • Use of silica cartridges or in-house prepared silica mini-columns for separation of degradation compounds.
  • Specification of apparatus including taper-shaped flasks with a capacity of 25 ml or 50 ml, improving sample recovery compared to earlier versions.
  • Detailed procedures for silica gel preparation, moisture adjustment, and activation to ensure accurate chromatographic separation.

• Calculation Guidelines

  • Emphasizes the calculation of the relative content of pyropheophytin a for evaluating the degree of chlorophyll degradation.

• Amendments and Updates
  The 2016 amendment provides clarifications on silica gel column preparation and modifies flask sizes to optimize sample handling, reflecting best laboratory practices.

Applications

• Quality Control in Vegetable Oil Production
  Producers use this standard to monitor degradation in vegetable oils caused by heat treatment, ensuring product quality and consistency.

• Shelf-Life and Freshness Assessment
  Analytical results support decisions on storage conditions and shelf-life estimations by quantifying the extent of chlorophyll breakdown.

• Regulatory Compliance and Imports
  Conformity with EN ISO 29841:2014/A1:2016 assists companies in meeting regulatory requirements for oil quality across European markets.

• Research and Development
  The standard serves as a foundation for further studies on the impact of processing and storage on oil pigments, aiding in developing improved refining methods.

Related Standards

• ISO 29841:2009
  The base international standard for determining chlorophyll degradation products in vegetable fats and oils, of which EN ISO 29841:2014/A1:2016 is an amended version.

• CEN/TC 307 Standards
  Covers methods of sampling and analysis for oilseeds, vegetable, and animal fats and oils, complementing chlorophyll degradation analysis.

• ISO/TC 34 Series
  Broader food product standards where ISO/TC 34/SC 11 manages standards related to animal and vegetable fats and oils.

Keywords

Vegetable fats and oils, chlorophyll degradation, pheophytins, pyropheophytins, oil quality standard, thermal degradation analysis, oil pigment analysis, silica gel chromatography, EN ISO 29841, CEN standard.

Standards Content (Sample)

SLOVENSKI STANDARD
01-april-2016
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Vegetable fats and oils - Determination of the degradation products of chlorophylls a and
a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009/AMD 1:2016)
Pflanzliche Fette und Öle - Bestimmung thermischer Abbauprodukte des Chlorophyll a
und a' (Pheophytin a, a' und Pyropheophytin) (ISO 29841:2009/AMD 1:2016)
Corps gras d'origine végétale - Détermination des produits de décomposition des
chlorophylles a et a' (phéophytines a, a' et pyrophéophytines) (ISO 29841:2009/AMD
1:2016)
Ta slovenski standard je istoveten z: EN ISO 29841:2014/A1:2016
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 29841:2014/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2016
EUROPÄISCHE NORM
ICS 67.200.10
English Version
Vegetable fats and oils - Determination of the degradation
products of chlorophylls a and a' (pheophytins a, a' and
pyropheophytins) (ISO 29841:2009/AMD 1:2016)
Corps gras d'origine végétale - Détermination des Pflanzliche Fette und Öle - Bestimmung thermischer
produits de décomposition des chlorophylles a et a' Abbauprodukte des Chlorophyll a und a' (Pheophytin a,
(phéophytines a, a' et pyrophéophytines) (ISO a' und Pyropheophytin) (ISO 29841:2009/AMD
29841:2009/AMD 1:2016) 1:2016)
This amendment A1 modifies the European Standard EN ISO 29841:2014; it was approved by CEN on 15 January 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 29841:2014/A1:2016 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
European foreword
This document (EN ISO 29841:2014/A1:2016) has been prepared by Technical Committee ISO/TC 34
“Food products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and
animal fats and oils and their by-products - Methods of sampling and analysis” the secretariat of which
is held by AFNOR.
This Amendment to the European Standard EN ISO 29841:2014 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by August 2016, and
conflicting national standards shall be withdrawn at the latest by August 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands,
Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 29841:2009/Amd 1:2016 has been approved by CEN as EN ISO 29841:2014/A1:2016
without any modification.
INTERNATIONAL ISO
STANDARD 29841
First edition
...