ISO 29841:2009/Amd 1:2016

Vegetable fats and oils — Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) — Amendment 1

Corps gras d'origine végétale — Détermination des produits de décomposition des chlorophylles a et a' (phéophytines a, a' et pyrophéophytines) — Amendement 1

General Information

• Status: Published
• Publication Date: 27-Jan-2016
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: ISO/TC 34/SC 11 - Animal and vegetable fats and oils
• Drafting Committee: ISO/TC 34/SC 11 - Animal and vegetable fats and oils
• Current Stage: 6060 - International Standard published
• Start Date: 28-Jan-2016
• Due Date: 03-Apr-2017
• Completion Date: 03-Apr-2017

Relations

• Consolidates: EN ISO 29841:2014/A1:2016 - Vegetable fats and oils - Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009/AMD 1:2016)
• Effective Date: 12-Feb-2026
• Amends: ISO 29841:2009 - Vegetable fats and oils — Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins)
• Effective Date: 27-Nov-2021

Overview

ISO 29841:2009/Amd 1:2016 is an amendment to the international standard developed by ISO for vegetable fats and oils. This amendment specifically addresses updates in the procedures for the determination of degradation products of chlorophylls a and a′-notably pheophytins a, a′ and pyropheophytins. Accurate analysis of these compounds is crucial for the quality assessment, processing control, and authenticity verification in vegetable oils. The amendment introduces refined details regarding laboratory apparatus, sample preparation, and calculation methods, supporting more reliable and reproducible results in analytical laboratories.

Key Topics

• Updated Laboratory Equipment
  • Specifies the use of taper-shaped flasks (25 ml or 50 ml) instead of smaller sizes to help reduce sample loss during rotary evaporation.
• Refined Chromatographic Separation
  • Provides options for silica or diol cartridges for effective separation of chlorophyll degradation products.
  • Details preparation and activation of in-house silica mini-columns, including guidance on silica gel characteristics and conditioning.
• Analytical Calculation Methods
  • Clarifies that calculations should be based on the relative content of pyropheophytin a, ensuring accuracy in reportable results.

Applications

Implementation of ISO 29841:2009/Amd 1:2016 is significant across various sectors in the vegetable fats and oils industry. Key applications include:

• Quality Assurance in Oil Production
  • Monitoring the levels of pheophytins and pyropheophytins helps identify refining process efficacy and oil freshness.
• Authentication and Fraud Detection
  • The standard supports the validation of oil authenticity, crucial for protecting against adulteration and mislabeling, especially in premium oils like extra virgin olive oil.
• Research and Product Development
  • Laboratories use these methods for scientific studies, process improvement, and the development of new oil products.
• Regulatory Compliance
  • Adoption of standardized methods ensures compliance with international food safety and trade regulations, facilitating global commerce.

Related Standards

Understanding ISO 29841:2009/Amd 1:2016 can be enriched by reference to associated standards in the domain of edible fats and oils:

• ISO 29841:2009 - The base standard outlining the methodology for the determination of chlorophyll degradation products.
• ISO 660 - Specifies acid value and acidity for animal and vegetable fats and oils.
• ISO 3960 - Relates to the determination of peroxide value to assess rancidity.
• ISO/TC 34/SC 11 - The relevant ISO technical committee and subcommittee focused on standards for animal and vegetable fats and oils.

Practical Value

By utilizing the updated methods provided in ISO 29841:2009/Amd 1:2016, laboratories and producers benefit from:

• Enhanced Accuracy in the detection of key quality markers for vegetable oils.
• Improved Consistency thanks to clearer equipment requirements and detailed sample preparation guidance.
• Stronger Consumer Protection and Trust by supporting claims of product authenticity and purity.

Adopting this ISO standard amendment helps ensure best practices and international alignment in the analysis of chlorophyll degradation products within the vegetable fats and oils industry.

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 29841
First edition
2009-03-15
AMENDMENT 1
2016-02-01
Vegetable fats and oils —
Determination of the degradation
products of chlorophylls a
and a’ (pheophytins a, a’ and
pyropheophytins)
AMENDMENT 1
Corps gras d’origine végétale — Détermination des produits de
décomposition des chlorophylles a et a’ (phéophytines a, a’ et
pyrophéophytines)
AMENDEMENT 1
Reference number
ISO 29841:2009/Amd.1:2016(E)
©
ISO 2016
ISO 29841:2009/Amd.1:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. [phone]
Fax [phone]
[e-mail]
www.iso.org
ii © ISO 2016 – All rights reserved

ISO 29841:2009/Amd.1:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
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Any trade name used in this document is information given for the convenience of users a
...

NORME ISO
INTERNATIONALE 29841
Première édition
2009-03-15
AMENDEMENT 1
2016-02-01
Corps gras d’origine végétale —
Détermination des produits de
décomposition des chlorophylles
a et a’ (phéophytines a, a’ et
pyrophéophytines)
AMENDEMENT 1
Vegetable fats and oils — Determination of the degradation products
of chlorophylls a and a’ (pheophytins a, a’ and pyropheophytins)
AMENDMENT 1
Numéro de référence
ISO 29841:2009/Amd.1:2016(F)
©
ISO 2016
ISO 29841:2009/Amd.1:2016(F)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2016, Publié en Suisse
Droits de reproduction réservés. Sauf indication contraire, aucune partie de cette publication ne peut être reproduite ni utilisée
sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique, y compris la photocopie, l’affichage sur
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l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. [phone]
Fax [phone]
[e-mail]
www.iso.org
ii © ISO 2016 – Tous droits réservés

ISO 29841:2009/Amd.1:2016(F)
Avant-propos
L’ISO (Organisation internationale de normalisation) est une fédération mondiale d’organismes
nationaux de normalisation (comités membres de l’ISO). L’élaboration des Normes internationales est
en général confiée aux comités techniques de l’ISO. Chaque comité membre intéressé par une étude
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gouvernementales et non gouvernementales, en liaison avec l’ISO participent également aux travaux.
L’ISO collabore étroitement avec la Commission électrotechnique internationale (IEC) en ce qui
concerne la normalisation électrotechnique.
Les procédures utilisées pour élaborer le présent document et celles destinées à sa mise à jour sont
décrites dans les Directives ISO/IEC, Partie 1. Il convient, en particulier de prendre note des différents
critères d’approbation requis pour les différents types de documents ISO. Le présent document a été
rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir www.
iso.org/directives).
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droits de propriété intellectuelle ou de droits analogues. L’ISO ne saurait être tenue pour responsable
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les références aux droits de propriété intellectuelle ou autres droits analogues identifiés lors de
l’élaboration du document sont indiqués dans l’Introduction et/ou dans la liste des déclarations de
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Les appellations commerciales éventuellement mentionnées dans le présent document sont données
pour information, par souci de commodité, à l’intention des utilisateurs et n
...