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EN ISO 21149:2009

(Main)

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2006)

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2006)

ISO 21149:2006 gives general guidelines for enumeration and detection of mesophilic aerobic bacteria present in cosmetics:
by counting the colonies on agar medium after aerobic incubation, or
by checking the absence of bacterial growth after enrichment.
Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise validated.
If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.

Kosmetik - Mikrobiologie - Zählung und Nachweis von aeroben mesophilen Bakterien (ISO 21149:2006)

Diese Internationale Norm gibt allgemeine Anleitungen für die Zählung und zum Nachweis von in Kosmetika
vorhandenen mesophilen aeroben Bakterien,
- durch Zählen der Kolonien auf einem Agarmedium nach aerober Bebrütung oder
- durch Überprüfen des Ausbleibens von bakteriellem Wachstum nach Anreicherung.
Wegen der großen Vielfalt an kosmetischen Mitteln innerhalb dieses Anwendungsbereiches braucht dieses
Verfahren nicht zwangsläufig bis in alle Einzelheiten auf bestimmte Mittel anwendbar zu sein (z. B. bestimmte
mit Wasser nicht mischbare Produkte). Ersatzweise können für die hier aufgeführten Untersuchungen andere
Verfahren (z. B. automatisierte) zur Anwendung kommen, vorausgesetzt, dass deren Gleichwertigkeit nachgewiesen
oder das Verfahren anderweitig validiert wurde.
Falls es erforderlich sein sollte, können ausgezählte oder nachgewiesene Mikroorganismen mit geeigneten
Identifizierungsprüfungen bestimmt werden, die in den unter den Literaturhinweisen aufgeführten Normen
beschrieben sind.
Um die Qualität des Produkts und die Sicherheit für Verbraucher sicherzustellen, ist es ratsam, eine geeignete
mikrobiologische Risikoanalyse zur Bestimmung der Arten von kosmetischen Mitteln durchzuführen, für die
diese Internationale Norm anwendbar ist. Produkte, die ein geringes mikrobiologisches Risiko darstellen, umfassen
Produkte mit geringer Wasseraktivität, alkoholisch-wässrige Produkte, Produkte mit extremen pH-Werten
usw.

Cosmétiques - Microbiologie - Dénombrement et détection des bactéries aérobies mésophiles (ISO 21149:2006)

L'ISO 21149:2006 donne des lignes directrices générales pour le dénombrement et la détection des bactéries aérobies mésophiles présentes dans les cosmétiques:
par dénombrement des colonies en milieu gélosé après une incubation aérobie, ou
en vérifiant l'absence de croissance bactérienne après enrichissement.
En raison de la grande variété de produits cosmétiques entrant dans ce domaine d'application, la présente méthode peut ne pas être, en tout point, adaptée à certains produits (par exemple à certains produits non miscibles dans l'eau). Il est possible de remplacer les essais présentés ici par d'autres méthodes (automatisées, par exemple), sous réserve que leur équivalence ait été démontrée ou que la méthode ait été validée par ailleurs.
Au besoin, les micro-organismes dénombrés ou détectés peuvent être identifiés à l'aide d'essais d'identification appropriés décrits dans les normes indiquées dans la Bibliographie.

Kozmetika - Mikrobiologija - Ugotavljanje prisotnosti in števila aerobnih mezofilnih bakterij (ISO 21149:2006)

General Information

Status
Withdrawn
Publication Date
16-Jun-2009
Withdrawal Date
20-Jun-2017
ICS
07.100.40 - Cosmetics microbiology
07.100.99 - Other standards related to microbiology
71.100.70 - Cosmetics. Toiletries
Technical Committee
CEN/TC 392 - Cosmetics
Drafting Committee
CEN/TC 392 - Cosmetics
Current Stage
9960 - Withdrawal effective - Withdrawal
Completion Date
21-Jun-2017
Mandate
M/426 - Standardisation mandate assigned to CEN concerning the microbiological analysis of cosmetic products
Ref Project
SIST EN ISO 21149:2009 - Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2006)

Relations

Replaced By
EN ISO 21149:2017 - Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2017)
Effective Date
24-Aug-2016

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 21149:2009
01-oktober-2009
Kozmetika - Mikrobiologija - Ugotavljanje prisotnosti in števila aerobnih mezofilnih
bakterij (ISO 21149:2006)
Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria
(ISO 21149:2006)
Kosmetik - Mikrobiologie - Zählung und Nachweis von aeroben mesophilen Bakterien
(ISO 21149:2006)
Cosmétiques - Microbiologie - Dénombrement et détection des bactéries aérobies
mésophiles (ISO 21149:2006)
Ta slovenski standard je istoveten z: EN ISO 21149:2009
ICS:
07.100.40 Kozmetika - mikrobiologija Cosmetics microbiology
SIST EN ISO 21149:2009 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 21149:2009

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SIST EN ISO 21149:2009
EUROPEAN STANDARD
EN ISO 21149
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2009
ICS 07.100.99; 71.100.70
English Version
Cosmetics - Microbiology - Enumeration and detection of
aerobic mesophilic bacteria (ISO 21149:2006)
Cosmétiques - Microbiologie - Dénombrement et détection Kosmetik - Mikrobiologie - Zählung und Nachweis von
des bactéries aérobies mésophiles (ISO 21149:2006) aeroben mesophilen Bakterien (ISO 21149:2006)
This European Standard was approved by CEN on 23 May 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21149:2009: E
worldwide for CEN national Members.

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SIST EN ISO 21149:2009
EN ISO 21149:2009 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 21149:2009
EN ISO 21149:2009 (E)
Foreword
The text of ISO 21149:2006 has been prepared by Technical Committee ISO/TC 217 “Cosmetics” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 21149:2009 by
Technical Committee CEN/SS H99 “Products for household and leisure use - Undetermined” the secretariat of
which is held by CMC.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by December 2009, and conflicting national standards shall be withdrawn
at the latest by December 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 21149:2006 has been approved by CEN as a EN ISO 21149:2009 without any modification.

3

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SIST EN ISO 21149:2009

---------------------- Page: 6 ----------------------

SIST EN ISO 21149:2009

INTERNATIONAL ISO
STANDARD 21149
First edition
2006-03-01


Cosmetics — Microbiology —
Enumeration and detection of aerobic
mesophilic bacteria
Cosmétiques — Microbiologie — Dénombrement et détection des
bactéries aérobies mésophiles





Reference number
ISO 21149:2006(E)
©
ISO 2006

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