EN 13435:2001

Surface active agents - Determination of free amine content of alkyl dimethyl betaines

This European Standard specifies a method for the determination of 0,02 mmol to 1 mmol of free amine in alkyl dimethyl betaines. Monochloroacetic acid, glycolic acid and strong acids do not interfere the determination.

Grenzflächenaktive Stoffe - Bestimmung des freien Amingehaltes von Alkyldimethylbetainen

Diese Europäische Norm legt ein Verfahren zur Bestimmung von 0,02 mmol bis 1 mmol freiem Amin in Alkyldimethylbetainen fest. Monochloressigsäure, Glycolsäure und starke Säuren stören die Bestimmung nicht.

Agents de surface - Détermination de la teneur en amines libres dans les alkyl diméthylbétaïnes

La présente norme européenne spécifie une méthode de détermination des amines libres présentes entre 0,02 mmol et 1 mmol dans les alkyldiméthylbétaïnes. L'acide monochloroacétique, l'acide glycolique et les acides forts n'interfèrent pas.

Površinsko aktivne snovi - Določevanje prostega amina v alkil dimetil betainih

General Information

• Status: Published
• Publication Date: 11-Dec-2001
• Withdrawal Date: 29-Jun-2002
• ICS: 71.100.40 - Surface active agents
• Technical Committee: CEN/TC 276 - Surface active agents
• Drafting Committee: CEN/TC 276/WG 1 - Methods of analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 31-Oct-2024
• Completion Date: 14-Apr-2025
• Ref Project: SIST EN 13435:2002 - Surface active agents - Determination of free amine content of alkyl dimethyl betaines

Overview

EN 13435:2001 is a European Standard developed by CEN that specifies a precise analytical method for the determination of free amine content in alkyl dimethyl betaines, a class of surface active agents. This standard covers the quantification of free amines within the range of 0.02 mmol to 1 mmol. The method is robust and unaffected by common interfering substances such as monochloroacetic acid, glycolic acid, and strong acids, ensuring accurate and reliable results critical for quality control and compliance in surfactant manufacturing.

Key Topics

• Scope and Purpose
  The standard targets the accurate measurement of free amine concentration in alkyl dimethyl betaines, essential components in detergents and personal care products. Free amines can affect the chemical stability and performance of these agents.

• Analytical Principle
  The method dissolves the sample in aqueous propan-2-ol, converts all components to the basic form using sodium hydroxide, and removes carbon dioxide by nitrogen purging. Free amines are then quantified by potentiometric titration with hydrochloric acid to detect end points using a combined glass/calomel electrode.

• Reagents and Apparatus
  Utilizes high-purity reagents such as standard hydrochloric acid solution, sodium hydroxide, thymol blue indicator, tri-n-butylamine, and nitrogen gas. Analytical balance and potentiometric titrating apparatus with specific electrodes are required for precise titration.

• Sampling and Sample Preparation
  Samples must be prepared following ISO 607 guidelines and, if solid or pasty, gently melted at low temperatures. This ensures a homogeneous sample suitable for titration.

• Calculation and Reporting
  The free amine content is calculated based on titration volume differences between sample and blank, expressed as grams per 100 grams to one decimal place. The test report must include sample identification, reference to the standard, results, and any deviations or observations during testing.

• Precision and Reliability
  The method demonstrates high repeatability and reproducibility, validated through ring tests involving multiple laboratories. Repeatability and reproducibility limits ensure confidence in results within specified tolerances.

Applications

• Quality Control in Surfactant Production
  EN 13435:2001 is primarily applied by manufacturers of surface active agents to monitor and control free amine content, which impacts product safety, efficacy, and compliance with regulatory requirements.

• Raw Material Characterization
  Enables chemical suppliers to certify alkyl dimethyl betaine batches regarding free amine levels, supporting transparency and specification conformity.

• Research and Development
  Provides a reliable analytical technique for R&D laboratories focusing on new surfactant formulations or optimizing production parameters.

• Regulatory Compliance
  Serves as a reference method for establishing compliance with European chemical standards and safety regulations related to surfactants.

Related Standards

• ISO 607 – Surface active agents and detergents – Methods of sample division: Defines sampling procedures crucial for preparing representative samples analyzed under EN 13435.

• EN ISO 3696 – Water for analytical laboratory use – Specification and test methods: Specifies water quality standards ensuring reagent purity and accuracy in titrations.

• ISO 5725-2 – Accuracy (trueness and precision) of measurement methods and results: Provides methodologies for evaluating repeatability and reproducibility, referenced in the validation of EN 13435 test precision.

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By adhering to EN 13435:2001, laboratories and manufacturers ensure precise determination of free amine content in alkyl dimethyl betaines, supporting the production of high-quality surfactants with consistent chemical properties. This standard is essential for professionals engaged in surfactant analysis, chemical quality assurance, and regulatory compliance in the chemical and personal care industries.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Grenzflächenaktive Stoffe - Bestimmung des freien Amingehaltes von AlkyldimethylbetainenAgents de surface - Détermination de la teneur en amines libres dans les alkyl diméthylbétainesSurface active agents - Determination of free amine content of alkyl dimethyl betaines71.100.40Površinsko aktivna sredstvaSurface active agentsICS:Ta slovenski standard je istoveten z:EN 13435:2001SIST EN 13435:2002en01-junij-2002SIST EN 13435:2002SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 13435December 2001ICS 71.100.40English versionSurface active agents - Determination of free amine content ofalkyl dimethyl betainesAgents de surface - Détermination de la teneur en amineslibres dans les alkyl diméthylbétaïnesGrenzflächenaktive Stoffe - Bestimmung des freienAmingehaltes von AlkyldimethylbetainenThis European Standard was approved by CEN on 11 November 2001.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2001 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 13435:2001 ESIST EN 13435:2002

Ring test results.7Annex B (informative)
Instrument settings.8Annex C (informative)
Example for a typical titration curve and its derivative.9Bibliography.10SIST EN 13435:2002
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