EN ISO 10872:2021

Water and soil quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

This document specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans. The method applies to contaminated whole freshwater sediment (maximum salinity 5 g/l), soil and waste, as well as to pore water, elutriates and aqueous extracts that were obtained from contaminated sediment, soil and waste.

Wasserbeschaffenheit und Bodenbeschaffenheit - Bestimmung der toxischen Wirkung von Sediment- und Bodenproben auf Wachstum, Fertilität und Reproduktion von Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

Dieses Dokument legt ein Verfahren zur Bestimmung der Toxizität von Umweltproben auf Wachstum, Fertilität und Reproduktion von Caenorhabditis elegans fest. Das Verfahren kann sowohl für Süßwassersediment (maximale Salinität 5 g/l), Böden, Abfall, als auch für Porenwasser, Eluate und wässrige Extrakte verwendet werden, die aus kontaminierten Sedimenten, Böden und Abfall extrahiert wurden.
WARNUNG - Anwender dieses Dokuments sollten mit der üblichen Laborpraxis vertraut sein. Dieses Dokument gibt nicht vor, alle unter Umständen mit der Anwendung des Verfahrens verbundenen Sicherheitsaspekte anzusprechen. Es liegt in der Verantwortung des Arbeitgebers, angemessene Sicherheits- und Schutzmaßnahmen zu treffen und sicherzustellen.
WICHTIG - Es ist erforderlich, bei den Untersuchungen nach diesem Dokument Fachleute oder Facheinrichtungen einzuschalten.

Qualité de l'eau et du sol - Détermination de l'effet toxique d'échantillons de sédiment et de sol sur la croissance, la fertilité et la reproduction de Caenorhabditis elegans (Nematodes) (ISO 10872:2020)

Le présent document spécifie une méthode de détermination de la toxicité d'échantillons environnementaux sur la croissance, la fertilité et la reproduction de Caenorhabditis elegans. La méthode s'applique aux sédiments d'eau douce contaminés (salinité maximale de 5 g/l), sols et déchets, ainsi qu'à l'eau interstitielle, aux élutriats et aux extraits aqueux obtenus à partir de sédiments contaminés, sols et déchets.

Kakovost vode in tal - Določanje učinkov strupenosti vzorcev usedlin in tal na rast, plodnost in razmnoževanje Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

General Information

• Status: Published
• Publication Date: 03-Aug-2021
• Withdrawal Date: 27-Feb-2022
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230/WG 2 - Biological methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 04-Aug-2021
• Due Date: 03-May-2023
• Completion Date: 04-Aug-2021
• Directive: 2000/60/EC - Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy (substitutes Directive Reference 01/991 - Common Position on WFD)
• Ref Project: SIST EN ISO 10872:2021 - Water and soil quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2021
Nadomešča:
SIST ISO 10872:2011
Kakovost vode in tal - Določanje učinkov strupenosti vzorcev usedlin in tal na
rast, plodnost in razmnoževanje Caenorhabditis elegans (Nematoda) (ISO
10872:2020)
Water and soil quality - Determination of the toxic effect of sediment and soil samples on
growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO
10872:2020)
Wasserbeschaffenheit - Bestimmung der toxischen Wirkung von Sediment- und
Bodenproben auf Wachstum, Fertilität und Reproduktion von Caenorhabditis elegans
(Nematoda) (ISO 10872:2020)
Qualité de l'eau et du sol - Détermination de l'effet toxique d'échantillons de sédiment et
de sol sur la croissance, la fertilité et la reproduction de Caenorhabditis elegans
(Nematodes) (ISO 10872:2020)
Ta slovenski standard je istoveten z: EN ISO 10872:2021
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 10872
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2021
EUROPÄISCHE NORM
ICS 13.060.70
English Version
Water and soil quality - Determination of the toxic effect of
sediment and soil samples on growth, fertility and
reproduction of Caenorhabditis elegans (Nematoda) (ISO
10872:2020)
Qualité de l'eau et du sol - Détermination de l'effet Wasserbeschaffenheit und Bodenbeschaffenheit -
toxique d'échantillons de sédiment et de sol sur la Bestimmung der toxischen Wirkung von Sediment-
croissance, la fertilité et la reproduction de und Bodenproben auf Wachstum, Fertilität und
Caenorhabditis elegans (Nematodes) (ISO Reproduktion von Caenorhabditis elegans (Nematoda)
10872:2020) (ISO 10872:2020)
This European Standard was approved by CEN on 18 July 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10872:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 10872:2020 has been prepared by Technical Committee ISO/TC 147 "Water quality” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 10872:2021
by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2022, and conflicting national standards
shall be withdrawn at the latest by February 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 10872:2020 has been approved by CEN as EN ISO 10872:2021 without any modification.

INTERNATIONAL ISO
STANDARD 10872
Second edition
2020-05
Water and soil quality —
Determination of the toxic effect
of sediment and soil samples on
growth, fertility and reproduction of
Caenorhabditis elegans (Nematoda)
Qualité de l'eau et du sol — Détermination de l'effet toxique
d'échantillons de sédiment et de sol sur la croissance, la fertilité et la
reproduction de Caenorhabditis elegans (Nematodes)
Reference number
ISO 10872:2020(E)
©
ISO 2020
ISO 10872:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii © ISO 2020 – All rights reserved

ISO 10872:2020(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents and media . 3
6 Apparatus . 5
7 Reference substance . 6
8 Organisms . 7
8.1 Test organism . 7
8.2 Food organism . 7
9 Stock- and pre-cultures . 7
9.1 Stock cultures . 7
9.1.1 Caenorhabditis elegans . 7
9.1.2 Escherichia coli. 7
9.2 Pre-culture . 8
10 Procedure. 8
10.1 Preparation of food medium . 8
10.2 Preparation of test material and controls . 8
10.2.1 Soil . 8
10.2.2 Sediment . 9
10.2.3 Pore water, elutriate, extract. 9
10.2.4 Solution of reference substance . . 9
10.3 Test .10
10.4 Nematode separation .10
10.5 Measurements and calculations .10
10.5.1 Recovery .10
10.5.2 Males .10
10.5.3 Growth and fertility .11
10.5.4 Reproduction .11
10.6 Timetable of the test .12
11 Validity criteria .13
12 Expression of results .13
13 Test report .14
Annex A (informative) Determination of maximal water holding capacity (WHC ) .15
max
Annex B (informative) Figures and photos of adult worms C. elegans .16
Annex C (informative) Micropipette method (10.3) .18
Annex D (informative) Performance data .19
Bibliography .
...