ISO 24829:2026

• Abstract:

  This document specifies the determination of aldehydes and ketones content in polyether polyols and polymer polyols by high performance liquid chromatography (HPLC). This document is applicable to the determination of formaldehyde (HCHO), acetaldehyde (CH3CHO), acrolein (CH2=CHCHO) and acetone (CH3COCH3) in polyether polyols and polymer polyols.

• Status: Published
• Publication Date: 11-Jun-2026
• ICS: 83.080.20 - Thermoplastic materials
• Technical Committee: ISO/TC 61/SC 12 - Thermosetting materials
• Drafting Committee: ISO/TC 61/SC 12/WG 6 - Polyurethane raw materials
• Current Stage: 6060 - International Standard published
• Start Date: 12-Jun-2026
• Due Date: 17-May-2027
• Completion Date: 12-Jun-2026

ISO 24829:2026 is the first edition of an ISO International Standard for measuring aldehydes and ketones in polyether polyols and polymer polyols. It specifies an HPLC method after DNPH derivatization for formaldehyde, acetaldehyde, acrolein and acetone, so laboratories can report these low-level carbonyl compounds in raw materials used for polyurethane applications.

What does ISO 24829:2026 specify?

ISO 24829:2026 specifies a test method for aldehydes and ketones in polyether polyols and polymer polyols by high performance liquid chromatography (HPLC). The document is organized into clauses on scope, normative references, terms and definitions, principle, reagents and materials, apparatus, procedure, calculation of results, precision, performance criteria and quality assurance, and the test report.

The scope is focused on formaldehyde, acetaldehyde, acrolein and acetone. In practice, that means the standard is for analytical laboratories that need a defined way to prepare the sample, run the chromatograph and report results in a consistent form.

What are the key requirements of ISO 24829:2026?

ISO 24829:2026 requires the analytes to be converted with 2,4-dinitrophenylhydrazine (DNPH) in acid before HPLC measurement. The derivatization creates stable hydrazone derivatives, which makes the carbonyl compounds easier to detect and quantify by their retention time and peak area.

Principle and detection

The document uses HPLC with UV detection or diode array detection, with the detection practice linked to ISO 16000-3. The chromatographic identification is based on comparison with DNPH derivative standards, so the laboratory has to match both the retention time and the peak response of each target compound.

Reagents, materials and apparatus

ISO 24829:2026 calls for analytical-grade reagents and specified laboratory glassware, including single-volume pipettes and one-mark volumetric flasks as defined in ISO 648 and ISO 1042. The apparatus section also requires an HPLC system with a C18 reverse-phase column, a data system, a water bath, a freezer for standard storage and a 0.22 μm hydrophobic needle filter. That matters because the method depends on clean sample handling and stable chromatographic separation.

Sample and calibration preparation

ISO 24829:2026 requires at least six calibration solutions covering the stated working range and a blank with each set of samples. Samples are weighed, dissolved, derivatized with DNPH and phosphoric acid, incubated in the dark, cooled and filtered before injection. This tells the operator exactly how to prepare matched standards and samples so the calibration curve and sample result are comparable.

Calibration and system check

The calibration curve is built by plotting peak area against concentration and using linear regression. If the linear correlation coefficient is 0.995 or less, the curve is prepared again. The standard also requires a batch check with a 0.5 μg/ml standard; if the relative deviation is greater than 5 %, the analyst recalibrates or prepares a new curve from fresh standards.

Calculation, precision and quality assurance

Results are reported in mg/kg from the sample and blank chromatograms using the stated calculation approach. The document also gives repeatability and recovery data, notes a lowest detection limit of 0.4 mg/kg, and says HPLC system performance shall follow ISO 16000-3. It also warns that sample loss can occur if the DNPH capacity is exceeded, so the analyst may need more DNPH solution or less sample.

What terms does ISO 24829:2026 define?

• Derivatization - the quantitative conversion of target compounds into easier-to-detect compounds by chemical reaction.
• Hydrazone derivative - the stable product formed when a carbonyl compound reacts with DNPH under acid conditions.
• High performance liquid chromatography (HPLC) - the separation and measurement method used to identify and quantify the target compounds.
• 2,4-dinitrophenylhydrazine (DNPH) - the reagent used to derivatize carbonyl compounds before chromatographic analysis.
• Repeatability - the within-laboratory precision reported in the standard’s precision section.
• Recovery - the proportion of added analyte that is measured again after the procedure.

Who uses ISO 24829:2026?

ISO 24829:2026 is used by laboratories that test polyether polyols and polymer polyols for carbonyl impurities. It is relevant to polyurethane raw-material suppliers, quality control teams, and contract testing labs that need reported values for formaldehyde, acetaldehyde, acrolein and acetone.

The introduction also links the standard to vehicle interior materials and automotive requirements, so it is useful for manufacturers and buyers supporting automotive OEM reporting. It helps when a customer specification or internal quality plan needs a documented HPLC method rather than an informal test approach.

Which standards are used with ISO 24829:2026?

• ISO 648 - provides the specification for single-volume pipettes used in preparing calibration and sample solutions.
• ISO 1042 - provides the specification for one-mark volumetric flasks used for solution preparation.
• ISO 16000-3 - is used for the HPLC detection approach and for system performance requirements.
• ISO 835 - is noted as a suitable reference for graduated pipettes.
• ISO 8655-2 - is noted as a suitable reference for piston-operated volumetric apparatus, including pipettes.

What does the ISO 24829:2026 document contain?

ISO 24829:2026 includes a reaction scheme for DNPH derivatization and a typical chromatogram of 13 carbonyl standards. It also provides representative HPLC conditions in Table 1, precision data in Tables 2 to 4, and the test report items to record. In practice, these figures and tables support method setup, calibration, verification of performance and reporting.

International
Standard
ISO 24829
First edition
Plastics — Polyether polyols and
2026-06
polymer polyols — Determination
of aldehydes and ketones
Plastiques — Polyols de polyéther et polyols de polymère —
Détermination des aldéhydes et des cétones
Reference number
© ISO 2026
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
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or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents and materials . 2
6 Apparatus . 2
7 Procedure . 4
7.1 Preparation of calibration solutions .4
7.2 Preparation of sample .4
7.3 Preparation of calibration curve .4
7.4 Analysis of sample .5
8 Calculation of results . 6
9 Precision . 6
9.1 Repeatability .6
9.2 Recovery .6
10 Performance criteria and quality assurance . 7
10.1 HPLC system performance .7
10.2 Sample loss .7
11 Test report . 7
Bibliography . 8

iii
Foreword
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iv
Introduction
Polyether polyols and polymer polyols are the main raw materials of polyurethane, which are widely used
in fields such as sofas, mattresses, car seats and headrests, adhesives, etc. In recent years, environmental
policies have become increasingly strict, with the rapid development of the automobile industry, the demand
of high-quality polyether and polymer polyols will increase greatly.
Automotive OEMs have limited requirements for formaldehyde, acetaldehyde and acrolein in the whole
vehicle and interior parts and materials. Therefore, the manufacturers of vehicle interior require the test
report of aldehydes and ketones content (generally including formaldehyde, acetaldehyde and acrolein)
in polyether polyols. In order to meet the high requirements of environmental policies and the needs of
sustainable development of the industry, it is necessary to establish a method for the determination of
aldehydes and ketones content in polyether polyols and polymer polyols.

v
International Standard ISO 24829:2026(en)
Plastics — Polyether polyols and polymer polyols —
Determination of aldehydes and ketones
1 Scope
This document specifies the determination of aldehydes and ketones content in polyether polyols and
polymer polyols by high performance liquid chromatography (HPLC).
This document is applicable to the determination of formaldehyde (HCHO), acetaldehyde (CH CHO), acrolein
(CH =CHCHO) and acetone (CH COCH ) in polyether polyols and polymer polyols.
2 3 3
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 648, Laboratory glassware — Single-volume pipettes
ISO 1042, Laboratory glassware — One-mark volumetric flasks
ISO 16000-3, Indoor air — Part 3: Determination of formaldehyde and other carbonyl compounds in indoor and
test chamber air — Active sampling method
3 Terms and definitions
For the purpose of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
derivatization
quantitative conversion of target compounds that are difficult to detect in the sample in
...