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ISO 11816-1:2024

(Main)

Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks

Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks

This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution. The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not higher than 7 000 mU/l.

Lait et produits laitiers — Détermination de l'activité de la phosphatase alcaline — Partie 1: Méthode fluorimétrique pour le lait et les boissons à base de lait

Le présent document spécifie une méthode fluorimétrique pour la détermination de l’activité de la phosphatase alcaline (ALP) (EC 3.1.3.1) dans les laits crus et traités thermiquement entiers, demi‑écrémés, écrémés et les laits aromatisés. La méthode est applicable au lait et aux boissons à base de lait de vache, de brebis et de chèvre. Elle est également applicable au lait en poudre après reconstitution. L’instrument utilisé pour la détermination de l’ALP peut lire des activités jusqu’à 7 000 mU/l (milliunités par litre). Si l’activité est supérieure à 7 000 mU/l, une dilution avec du lait exempt d’ALP est effectuée de façon à obtenir un mesurage ne dépassant pas 7 000 mU/l.

General Information

Status
Published
Publication Date
11-Jan-2024
ICS
67.100.10 - Milk and processed milk products
Technical Committee
ISO/TC 34/SC 5 - Milk and milk products
Drafting Committee
ISO/TC 34/SC 5 - Milk and milk products
Current Stage
6060 - International Standard published
Start Date
12-Jan-2024
Due Date
27-Oct-2023
Completion Date
12-Jan-2024
Ref Project

Relations

Consolidates
ISO 23722:2021 - Meat and meat products — Vocabulary
Effective Date
08-Oct-2022
Revises
ISO 11816-1:2013 - Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks
Effective Date
01-Oct-2022

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ISO/PRF 11816-1 - Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks Released:16. 10. 2023ISO/PRF 11816-1 - Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks Released:16. 10. 2023
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REDLINE ISO/PRF 11816-1 - Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks Released:16. 10. 2023REDLINE ISO/PRF 11816-1 - Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks Released:16. 10. 2023
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REDLINE ISO/PRF 11816-1 - Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks Released:16. 10. 2023
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Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 11816-1
IDF 155-1
Fourth edition
Milk and milk products —
Determination of alkaline
phosphatase activity —
Part 1:
Fluorimetric method for milk and
milk-based drinks
Lait et produits laitiers — Détermination de l'activité de la
phosphatase alcaline —
Partie 1: Méthode fluorimétrique pour le lait et les boissons à base de
lait
PROOF/ÉPREUVE
Reference numbers
ISO 11816-1:2023(E)
IDF 155-1:2023(E)
© ISO and IDF 2023

---------------------- Page: 1 ----------------------
ISO 11816-1:2023(E)
IDF 155-1:2023(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO and IDF 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: +41 22 749 01 11 Phone: +32 2 325 67 40
Fax: +32 2 325 67 41
Email: copyright@iso.org Email: info@fil-idf.org
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii
PROOF/ÉPREUVE © ISO and IDF 2023 – All rights reserved

---------------------- Page: 2 ----------------------
ISO 11816-1:2023(E)
IDF 155-1:2023(E)
Contents Page
Forewords .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparations . 4
8.1 Alkaline phosphatase-free milk . 4
8.2 Preparation of the test sample . 4
8.2.1 General . 4
8.2.2 Pasteurized test samples . 4
8.2.3 Dilution of test samples with high ALP values . 4
9 Procedure .4
9.1 Verification of instrument performance . 4
9.1.1 General .
...

© ISO and IDF 2023 – All rights reserved
Formatted: Font: 11 pt
revISO/DISISO 11816-1 | IDF 155-1:2023(E)
Formatted: Font: 11 pt, English (United Kingdom)
Date: 2023-08-0110-10
Formatted: Font: 11 pt
ISO/TC 34/SC 5
Formatted: Space After: 6 pt
Secretariat: NEN
Formatted: Font: 11 pt, Bold
Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric
Formatted: Font: 11 pt, Bold
method for milk and milk-based drinks
Formatted: Font: 11 pt
Lait et produits laitiers — Détermination de l’activité de la phosphatase alcaline — Partie 1: Méthode
Formatted: Right, Space Before: 0 pt, After: 6 pt
fluorimétrique pour le lait et les boissons à base de lait
Formatted: Font: 11 pt, French (Switzerland)
Formatted: Right, Space After: 6 pt
Formatted: Font: 11 pt, Bold, Not Italic, French
(Switzerland)

---------------------- Page: 1 ----------------------
© ISO and IDF 2023 – All rights reserved
© ISO and IDF 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this
publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical,
including photocopying, or posting on the internet or an intranet, without prior written permission. Permission
can be requested from either ISO at the address below or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: +41 22 749 01 11 Phone: + 32 2 325 67 40
Email: copyright@iso.org Fax: + 32 2 325 67 41
Website: www.iso.org Email: info@fil-idf.org
Website: www.fil-idf.org
Published in Switzerland

---------------------- Page: 2 ----------------------
revISO/DIS ISO 11816-1 | IDF 155-1:2023(E)
Formatted: Justified, Line spacing: Exactly 11 pt
Formatted: English (United Kingdom)
Formatted: English (United Kingdom)
© ISO and IDF 2023
Formatted: English (United Kingdom)
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no
part of this publication may be reproduced or utilized otherwise in any form or by any means,
electronic or mechanical, including photocopying, or posting on the internet or an intranet, without
prior written permission. Permission can be requested from either ISO at the address below or
ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
International Dairy Federation
Silver Building • Bd Auguste Reyers 70/B
B-1030 Brussels
Phone: + 32 2 325 67 40
Fax: + 32 2 325 67 41
Email: info@fil-idf.org
Website: www.fil-idf.org
Published in Switzerland
ii © ISO and IDF 2023 – All rights reserved

---------------------- Page: 3 ----------------------
revISO/DISISO 11816-1 | IDF 155-1:2023(E)
Formatted: Justified, Line spacing: Exactly 11 pt
Formatted: English (United Kingdom)
Formatted: English (United Kingdom)
Contents
Formatted: English (United Kingdom)
Forewords . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparations . 4
9 Procedure . 4
10 Calculation and expression of results .
...

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