SIST EN 13368-1:2014

Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography

This European Standard specifies a method for the chromatographic determination of the total amount of each of the individual chelating agents EDTA, HEEDTA, and DTPA in fertilizers containing one or more of these substances. The method allows the identification and the determination of the total water soluble fraction of each of these chelating agents. It does not allow to distinguish between the free form and the metal bound form of the chelating agents.
NOTE   EDTA, HEEDTA and DTPA are abbreviations used in this European Standard for the sake of simplicity. For complete names see Annex A.
This method applies to fertilizers containing chelates of one or more of the following micro-nutrients: cobalt, copper, iron, manganese, zinc and with a mass fraction of at least 0,1 %.

Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 1: Bestimmung von EDTA, HEEDTA und DTPA mit Ionenchromatographie

Diese Europäische Norm legt ein Verfahren zur chromatographischen Bestimmung des Gesamtgehalts von jedem einzelnen der Chelatbildner EDTA, HEEDTA und DTPA in Düngemitteln fest, die eine oder mehrere dieser Substanzen enthalten. Das Verfahren ermöglicht die Identifizierung und Bestimmung des Gesamtanteils an wasserlöslichen Bestandteilen jedes dieser Chelatbildner. Es erlaubt keine Unterscheidung zwischen der freien Form und der metallisch gebundenen Form der Chelatbildner.
ANMERKUNG EDTA, HEEDTA und DTPA sind Abkürzungen, die in dieser Europäischen Norm der Einfachheit halber verwendet werden. Bezüglich der vollständigen Bezeichnungen siehe Anhang A.
Dieses Verfahren gilt für Düngemittel, die Chelate von einem oder mehreren der folgenden Spurennährstoffe enthalten: Kobalt, Kupfer, Eisen, Mangan, Zink mit einem Massenanteil von mindestens 0,1 %.

Engrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 1: Détermination du EDTA, HEEDTA et DTPA par chromatographie ionique

La présente norme européenne spécifie une méthode pour la détermination par chromatographie de la quantité totale de chacun des agents chélatants individuels EDTA, HEEDTA et DTPA dans les engrais contenant une ou plusieurs de ces substances. La méthode permet d’identifier et de déterminer la proportion totale soluble dans l’eau de chacun de ces agents chélatants. Elle ne permet pas de faire la distinction entre la forme libre et la forme métallique de l’agent chélatant.
NOTE   EDTA, HEEDTA et DTPA sont des abréviations employées dans la présente norme européenne dans un souci de simplicité : pour les noms complets, se reporter à l’annexe A.
La présente méthode s’applique aux engrais contenant des chélates d’au moins un des oligo-éléments suivants : cobalt, cuivre, fer, manganèse, zinc, avec une fraction massique d’au moins 0,1 %.

Gnojila - Določevanje sredstev za kelatiziranje v gnojilih s kromatografijo - 1. del: Določevanje EDTA, HEEDTA in DTPA z ionsko kromatografijo

General Information

• Status: Published
• Public Enquiry End Date: 09-Nov-2012
• Publication Date: 13-Feb-2014
• ICS: 65.080 - Fertilizers
• Technical Committee: KAT - Soil quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 05-Feb-2014
• Due Date: 12-Apr-2014
• Completion Date: 14-Feb-2014
• Ref Project: EN 13368-1:2014 - Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography

Relations

• Replaces: SIST EN 13368-1:2002 - Fertilizers - Determination of chelating agents in fertilizers by ion chromatography - Part 1: EDTA, HEDTA and DTPA
• Effective Date: 01-Mar-2014

Overview

SIST EN 13368-1:2014 is a European standard established by CEN that defines a precise chromatographic method for determining the content of specific chelating agents-EDTA, HEEDTA, and DTPA-in fertilizers. These chelating agents play a crucial role in nutrient delivery by stabilizing trace elements such as cobalt, copper, iron, manganese, and zinc. The standard focuses on the total water-soluble fraction of these chelating agents in fertilizers, using ion chromatography to ensure accurate identification and quantification.

This standard is essential for laboratories, manufacturers, and quality control entities within the fertilizer industry to ensure accurate determination of chelating agents, thereby guaranteeing product consistency and compliance with regulatory requirements.

Key Topics

• Scope and Purpose
  SIST EN 13368-1:2014 applies specifically to fertilizers containing at least 0.1% (mass fraction) of chelates of micro-nutrients complexed with EDTA, HEEDTA, or DTPA. The standard targets the combined measurement of free and metal-bound forms but does not differentiate between them.

• Analytical Principle
  The method works by substituting micro-nutrients in the aqueous fertilizer extract with iron(III) ions. The resulting iron chelates undergo separation through ion exchange chromatography using an anion exchange column and nitrate-acetate eluent at controlled pH. Detection relies on UV photometry at 330 nm, enhanced by a post-column reaction with diluted perchloric acid.

• Interferences and Limitations
  Various salts and compounds may interfere with the chromatographic process, including high concentrations of chloride and certain polyamino polycarboxylic acids. The method mitigates interference through the choice of column and specific reagent treatment protocols.

• Reagents and Equipment
  Utilizes analytical grade reagents such as nitric acid, sodium hydroxide, and specialized stock solutions of EDTA, HEEDTA, and DTPA. Essential apparatus includes a liquid chromatograph equipped with an isocratic pump, injection valve, anion exchange column, UV/VIS detector, and membrane filters with 0.2 μm and 0.45 μm porosities.

• Procedure Overview
  The standardized procedure involves sample extraction with water, replacement of native micro-nutrients by iron(III), filtration, and chromatographic analysis using calibration standards. Results are expressed as mass fractions of chelating agents calculated against calibration graphs.

Applications

• Quality Control in Fertilizer Production
  Manufacturers use SIST EN 13368-1:2014 to quantify critical chelating agents, essential for optimizing the bioavailability of micronutrients and ensuring product performance.

• Regulatory Compliance
  The standardized method supports compliance with European fertilizer legislation by providing a reliable, reproducible test for ingredient verification.

• Research and Development
  Fertilizer R&D labs apply this method to develop and optimize chelate formulations, improving nutrient efficiency and environmental safety.

• Agricultural Testing
  Enables testing laboratories to verify fertilizer content, thereby ensuring accurate nutrient delivery recommendations for agricultural use.

Related Standards

• EN 1482-1: Sampling
  Provides guidance on fertilizer sampling techniques which complement the sample preparation requirements in SIST EN 13368-1:2014.

• EN 1482-2: Sample Preparation
  Details procedures for preparing fertilizer samples prior to chromatographic analysis for accurate and consistent results.

• EN 12944 Series: Vocabulary
  Defines key terminology related to fertilizers and liming materials, supporting precise communication and understanding within the industry.

• EN ISO 3696: Water Quality
  Specifies the standards for laboratory water quality, which is critical for ensuring reproducibility and accuracy in chromatographic analysis.

Keywords

Fertilizers, chelating agents, EDTA determination, HEEDTA, DTPA, ion chromatography, nutrient analysis, micro-nutrients, quality control, fertilizer standards, CEN standard, fertilizer testing, chromatographic method, trace elements, agricultural fertilizers.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 1: Bestimmung von EDTA, HEEDTA und DTPA mit IonenchromatographieEngrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 1: Détermination du EDTA, HEEDTA et DTPA par chromatographie ioniqueFertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 13368-1:2014SIST EN 13368-1:2014en,fr,de01-marec-2014SIST EN 13368-1:2014SLOVENSKI
STANDARDSIST EN 13368-1:20021DGRPHãþD

EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 13368-1
January 2014 ICS 65.080 Supersedes EN 13368-1:2001English Version
Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography
Engrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 1: Détermination du EDTA, HEEDTA et DTPA par chromatographie ionique
Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 1: Bestimmung von EDTA, HEEDTA und DTPA mit IonenchromatographieThis European Standard was approved by CEN on 16 November 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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CEN-CENELEC Management Centre:
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B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 13368-1:2014 ESIST EN 13368-1:2014
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