SIST EN ISO 23753-1:2019/A1:2021

Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC) - Amendment 1 (ISO 23753-1:2019/Amd 1:2020)

Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 1: Verfahren mit Triphenyltetrazoliumchlorid (TTC) (ISO 23753-1:2019/Amd 1:2020)

No scope available

Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 1: Méthode au chlorure de triphényltétrazolium (CTT) - Amendement 1 (ISO 23753-1:2019/Amd 1:2020)

Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 1. del: Metoda s trifeniltetrazolijevim kloridom (TTC) - Dopolnilo A1 (ISO 23753-1:2019/Amd 1:2020)

General Information

• Status: Published
• Public Enquiry End Date: 03-May-2020
• Publication Date: 06-Dec-2020
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: KAT - Soil quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 23-Nov-2020
• Due Date: 28-Jan-2021
• Completion Date: 07-Dec-2020
• Ref Project: EN ISO 23753-1:2019/A1:2020 - Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC) - Amendment 1 (ISO 23753-1:2019/Amd 1:2020)

Relations

• Amends: SIST EN ISO 23753-1:2019 - Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC) (ISO 23753-1:2019)
• Effective Date: 01-Jan-2021

Overview

The SIST EN ISO 23753-1:2019/A1:2021 standard, titled Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC) - Amendment 1, is an important European and international standard published by CEN and ISO. This document specifies an amended analytical method for measuring enzymatic activity-specifically, dehydrogenase activity-in soil samples using the TTC assay.

Dehydrogenases are critical soil enzymes involved in biological oxidation processes and serve as indicators of overall microbial activity and soil health. Assessing their activity plays a fundamental role in environmental monitoring, soil quality evaluation, and ecological studies.

Key Topics

• Methodology
  The amended method quantifies dehydrogenase activity based on the reduction of triphenyltetrazolium chloride (TTC) to triphenyl formazan (TPF), which is measurable spectrophotometrically. The amendment updates the calculation formula for enzymatic activity, accounting for dry soil weight and other key variables.

• Formula for Activity Calculation
  Enzymatic activity (A) is calculated as follows:

  [ A = \frac{(C_s - C_b) \times V \times 100}{m \times DM \times RT} ]

  • C_s: concentration of TPF in sample replicate (nmol/ml)
  • C_b: concentration of TPF in control (nmol/ml)
  • V: reaction volume in ml (substrate + buffer + ethanol)
  • m: mass of soil sample (g)
  • DM: dry matter content of soil (%)
  • RT: reaction time (minutes)

• Standardization and Compliance
  The amendment is integrated into the original EN ISO 23753-1:2019 and must be implemented by all CEN member countries to ensure harmonization of soil quality assessment methods across Europe.

• Technical Committee Involvement
  The standard was developed by ISO/TC 190 "Soil quality" and CEN/TC 444 focused on environmental characterization of solid matrices, ensuring international consensus and scientific rigor.

Applications

• Soil Quality Monitoring
  Regular assessment of dehydrogenase activity provides vital data on soil microbial health, which relates directly to soil fertility and ecosystem functionality.

• Environmental Impact Assessment
  This method is widely used to evaluate the impact of pollutants, agricultural practices, and remediation efforts on soil biology.

• Agricultural Research
  Researchers employ this standardized enzymatic assay to investigate soil biological responses to different land management practices or inputs.

• Ecotoxicology Studies
  Measuring soil enzyme activities enables the detection of toxic substances affecting microbial populations, assisting in risk assessments.

• Policy and Regulatory Compliance
  Governments and environmental agencies adopt SIST EN ISO 23753-1:2019/A1:2021 as part of soil conservation and environmental protection protocols.

Related Standards

• ISO 23753 Series
  This amendment is part of the ISO 23753 series addressing various methods for determining soil dehydrogenase activities, including alternative reagents and protocols.

• ISO 11465
  Referenced for determining soil dry matter content, which is essential for accurate normalization of enzymatic activity.

• CEN/TC 444 Standards
  Covers broader environmental characterization methods for solid matrices including soils, sediments, and sludges.

• Other Soil Quality Standards
  Related standards on chemical, physical, and biological soil properties complement this enzymatic activity method to provide comprehensive soil quality profiles.

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Keywords: Soil quality standard, dehydrogenase activity, triphenyltetrazolium chloride, TTC assay, soil enzymes, microbial activity, enzymatic soil analysis, ISO 23753-1 amendment, soil biological properties, environmental monitoring, soil health assessment, CEN standards, soil quality testing method.

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2021
Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 1. del: Metoda s
trifeniltetrazolijevim kloridom (TTC) - Dopolnilo A1 (ISO 23753-1:2019/Amd 1:2020)
Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using
triphenyltetrazolium chloride (TTC) - Amendment 1 (ISO 23753-1:2019/Amd 1:2020)
Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 1:
Verfahren mit Triphenyltetrazoliumchlorid (TTC) (ISO 23753-1:2019/Amd 1:2020)
Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 1:
Méthode au chlorure de triphényltétrazolium (CTT) - Amendement 1 (ISO 23753-
1:2019/Amd 1:2020)
Ta slovenski standard je istoveten z: EN ISO 23753-1:2019/A1:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 23753-1:2019/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Determination of dehydrogenases activity in
soils - Part 1: Method using triphenyltetrazolium chloride
(TTC) - Amendment 1 (ISO 23753-1:2019/Amd 1:2020)
Qualité du sol - Détermination de l'activité des Bodenbeschaffenheit - Bestimmung der
déshydrogénases dans les sols - Partie 1: Méthode au Dehydrogenaseaktivität in Böden - Teil 1: Verfahren
chlorure de triphényltétrazolium (CTT) - Amendement mit Triphenyltetrazoliumchlorid (TTC) (ISO 23753-
1 (ISO 23753-1:2019/Amd 1:2020) 1:2019/Amd 1:2020)
This amendment A1 modifies the European Standard EN ISO 23753-1:2019; it was approved by CEN on 28 October 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23753-1:2019/A1:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 23753-1:2019/A1:2020) has been prepared by Technical Committee ISO/TC
190 "Soil quality" in collaboration with Technical Committee CEN/TC 444 “Environmental
characterization of solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2021, and conflicting national standards shall be
withdrawn at the latest by May 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 23753-1:2019/Amd 1:2020 ha
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