SIST EN ISO 11348-1:2009/A1:2019

Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 1: Method using freshly prepared bacteria - Amendment 1 (ISO 11348-1:2007/Amd 1:2018)

Wasserbeschaffenheit - Bestimmung der Hemmwirkung von Wasserproben auf die Lichtemission von Vibrio fischeri (Leuchtbakterientest) - Teil 1: Verfahren mit frisch gezüchteten Bakterien - Änderung 1 (ISO 11348-1:2007/Amd 1:2018)

No scope available

Qualité de l'eau - Détermination de l'effet inhibiteur d'échantillons d'eau sur la luminescence de Vibrio fischeri (Essai de bactéries luminescentes) - Partie 1: Méthode utilisant des bactéries fraîchement préparées - Amendement 1 (ISO 11348-1:2007/Amd 1:2018)

Kakovost vode - Določevanje zaviralnega učinka vzorcev vode na oddajanje svetlobe Vibrio fischeri (preskus luminiscence bakterije) - 1. del: Metoda z uporabo sveže pripravljenih bakterij - Dopolnilo A1 (ISO 11348-1:2007/Amd 1:2018)

General Information

• Status: Published
• Public Enquiry End Date: 30-Jun-2017
• Publication Date: 10-Apr-2019
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: KAV - Water quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 04-Feb-2019
• Due Date: 11-Apr-2019
• Completion Date: 11-Apr-2019
• Ref Project: EN ISO 11348-1:2008/A1:2018 - Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 1: Method using freshly prepared bacteria - Amendment 1 (ISO 11348-1:2007/Amd 1:2018)

Relations

• Amends: SIST EN ISO 11348-1:2009 - Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 1: Method using freshly prepared bacteria (ISO 11348-1:2007)
• Effective Date: 01-May-2019

Overview

SIST EN ISO 11348-1:2009/A1:2019 is a key European Standard developed by CEN in collaboration with ISO Technical Committees focused on water quality and water analysis. This amendment to the original ISO 11348-1:2007 revises the method for determining the inhibitory effect of water samples on the light emission of Vibrio fischeri, a luminescent bacterium commonly used in bioassays to assess water toxicity.

The standard specifies the Part 1 testing method using freshly prepared bacteria. It is essential for laboratories engaged in biological water quality testing, offering a reliable bioanalytical procedure to detect toxic substances by measuring the reduction in bacterial bioluminescence when exposed to contaminated water samples.

Key Topics

• Luminescent Bacteria Test: The standard utilizes Vibrio fischeri, bacteria that emit light naturally. The degree of inhibition of their light emission indicates the presence and concentration of toxic agents in water.

• Freshly Prepared Bacteria Method: This approach improves test accuracy and repeatability by using bacteria cultured shortly before the test, ensuring optimal responsiveness.

• Inhibitory Effect Measurement: The core of the method is quantifying how water samples reduce bacterial luminescence, expressed in light inhibition detected during the assay.

• Updated References: Amendment 1 replaces prior references to turbidity measurement with ISO 7027-1 to align with the latest quantitative methods.

• Precision Criteria: The amendment refines statistical acceptance criteria, specifying that deviations for test sample dilutions determining the LID (Luminous Intensity Decrease)-value should not exceed 3 percentage points, enhancing result reliability.

Applications

• Environmental Monitoring: SIST EN ISO 11348-1:2009/A1:2019 supports assessing water bodies such as rivers, lakes, and groundwater for pollutant presence by screening for toxic effects rapidly.

• Wastewater Treatment Control: The luminescent bacteria bioassay helps utilities monitor treatment efficacy and detect toxicity spikes before release into natural water courses.

• Industrial Effluent Testing: Industries discharging water are required to evaluate effluent toxicity; this standard method provides a validated testing approach.

• Regulatory Compliance: National and international water quality regulators recognize this standard for setting thresholds and assessing compliance with environmental protection laws.

• Research and Development: Scientists developing new water treatment technologies employ the test for toxicity screening during product validation.

Related Standards

• ISO 11348 Series: This standard is part 1 of the series covering different testing methodologies using Vibrio fischeri bioluminescence.

• ISO 7027-1: Referenced for turbidity measurement, critical for preparing and interpreting water samples in bioassays.

• CEN/TC 230 - Water Analysis Standards: Collaborative standards covering various physical, chemical, and biological water testing methods.

• ISO/TC 147 - Water Quality: The technical committee overseeing water quality standards, including biological methods.

Practical Value

Implementing SIST EN ISO 11348-1:2009/A1:2019 allows laboratories and environmental agencies to:

• Detect toxic pollutants quickly and economically using a biological indicator.

• Ensure highly reproducible test results due to standardized methodologies and updated reference procedures.

• Align with European harmonized standards to facilitate regulatory acceptance and international data comparability.

• Utilize a non-specific bioassay that covers a broad range of toxicants, supplementing chemical analyses.

For professionals engaged in environmental testing, wastewater management, and compliance monitoring, this standard is an indispensable tool for assessing water toxicity through luminescent bacteria assays.

Standards Content (Sample)

SLOVENSKI STANDARD
01-maj-2019
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Water quality - Determination of the inhibitory effect of water samples on the light
emission of Vibrio fischeri (Luminescent bacteria test) - Part 1: Method using freshly
prepared bacteria - Amendment 1 (ISO 11348-1:2007/Amd 1:2018)
Wasserbeschaffenheit - Bestimmung der Hemmwirkung von Wasserproben auf die
Lichtemission von Vibrio fischeri (Leuchtbakterientest) - Teil 1: Verfahren mit frisch
gezüchteten Bakterien - Änderung 1 (ISO 11348-1:2007/Amd 1:2018)
Qualité de l'eau - Détermination de l'effet inhibiteur d'échantillons d'eau sur la
luminescence de Vibrio fischeri (Essai de bactéries luminescentes) - Partie 1: Méthode
utilisant des bactéries fraîchement préparées - Amendement 1 (ISO 11348-1:2007/Amd
1:2018)
Ta slovenski standard je istoveten z: EN ISO 11348-1:2008/A1:2018
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11348-1:2008/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2018
EUROPÄISCHE NORM
ICS 13.060.70
English Version
Water quality - Determination of the inhibitory effect of
water samples on the light emission of Vibrio fischeri
(Luminescent bacteria test) - Part 1: Method using freshly
prepared bacteria - Amendment 1 (ISO 11348-
1:2007/Amd 1:2018)
Qualité de l'eau - Détermination de l'effet inhibiteur Wasserbeschaffenheit - Bestimmung der
d'échantillons d'eau sur la luminescence de Vibrio Hemmwirkung von Wasserproben auf die
fischeri (Essai de bactéries luminescentes) - Partie 1: Lichtemission von Vibrio fischeri
Méthode utilisant des bactéries fraîchement préparées (Leuchtbakterientest) - Teil 1: Verfahren mit frisch
- Amendement 1 (ISO 11348-1:2007/Amd 1:2018) gezüchteten Bakterien - Änderung 1 (ISO 11348-
1:2007/Amd 1:2018)
This amendment A1 modifies the European Standard EN ISO 11348-1:2008; it was approved by CEN on 18 December 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11348-1:2008/A1:2018 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11348-1:2008/A1:2018) has been prepared by Technical Committee ISO/TC
147 "Water quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2019, and conflicting national standards shall be
withdrawn at the latest by June 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germ
...