Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media - Amendment 2 (ISO 11133:2014/Amd 2:2020)

Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung, Lagerung und Leistungsprüfung von Nährmedien - Änderung 2 (ISO 11133:2014/Amd 2:2020)

Microbiologie des aliments, des aliments pour animaux et de l'eau - Préparation, production, stockage et essais de performance des milieux de culture - Amendement 2 (ISO 11133:2014/Amd 2:2020)

Mikrobiologija živil, krme in vode - Priprava, izdelava, skladiščenje in preskušanje lastnosti gojišč - Dopolnilo A2 (ISO 11133:2014/Amd 2:2020)

General Information

Status
Published
Public Enquiry End Date
04-Mar-2018
Publication Date
18-Jun-2020
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
08-Jun-2020
Due Date
13-Aug-2020
Completion Date
19-Jun-2020

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SLOVENSKI STANDARD
01-julij-2020
Mikrobiologija živil, krme in vode - Priprava, izdelava, skladiščenje in preskušanje
lastnosti gojišč - Dopolnilo A2 (ISO 11133:2014/Amd 2:2020)
Microbiology of food, animal feed and water - Preparation, production, storage and
performance testing of culture media - Amendment 2 (ISO 11133:2014/Amd 2:2020)
Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung,
Lagerung und Leistungsprüfung von Nährmedien - Änderung 2 (ISO 11133:2014/Amd
2:2020)
Microbiologie des aliments, des aliments pour animaux et de l'eau - Préparation,
production, stockage et essais de performance des milieux de culture - Amendement 2
(ISO 11133:2014/Amd 2:2020)
Ta slovenski standard je istoveten z: EN ISO 11133:2014/A2:2020
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11133:2014/A2
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2020
EUROPÄISCHE NORM
ICS 07.100.30; 07.100.20
English Version
Microbiology of food, animal feed and water - Preparation,
production, storage and performance testing of culture
media - Amendment 2 (ISO 11133:2014/Amd 2:2020)
Microbiologie des aliments, des aliments pour animaux Mikrobiologie von Lebensmitteln, Futtermitteln und
et de l'eau - Préparation, production, stockage et essais Wasser - Vorbereitung, Herstellung, Lagerung und
de performance des milieux de culture - Amendement Leistungsprüfung von Nährmedien - Änderung 2 (ISO
2 (ISO 11133:2014/Amd 2:2020) 11133:2014/Amd 2:2020)
This amendment A2 modifies the European Standard EN ISO 11133:2014; it was approved by CEN on 21 March 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11133:2014/A2:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11133:2014/A2:2020) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food
chain” the secretariat of which is held by AFNOR.
This Amendment to the European Standard EN ISO 11133:2014 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by November 2020,
and conflicting national standards shall be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 11133:2014/Amd 2:2020 has been approved by CEN as EN ISO 11133:2014/A2:2020
without any modification.
INTERNATIONAL ISO
STANDARD 11133
First edition
2014-05-15
AMENDMENT 2
2020-05
Microbiology of food, animal feed and
water — Preparation, production,
storage and performance testing of
culture media
AMENDMENT 2
Microbiologie des aliments, des aliments pour animaux et de l'eau —
Préparation, production, stockage et essais de performance des
milieux de culture
AMENDEMENT 2
Reference number
ISO 11133:2014/Amd.2:2020(E)
©
ISO 2020
ISO 11133:2014/Amd.2:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

ISO 11133:2014/Amd.2:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology, in collaboration with Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4,
Microbiological methods, and in collaboration with the European Committee for Standardization (CEN)
Technical Committee CEN/TC 463, Microbiology of the food chain, in accordance with the Agreement on
technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
ISO 11133:2014/Amd.2:2020(E)
Microbiology of food, animal feed and water —
Preparation, production, storage and performance testing
of culture media
AMENDMENT 2
End of 5.2 (before the NOTE)
Add the following sentence:
Annex K gives the test microorganisms to be used for confirmation media and reagents in specified
food and water microbiology International Standards.

5.4.1, first sentence
Replace the sentence with the following text:
Suitable microorganisms for routine performance testing are listed in Annexes E, F and K.
End of 6.6.1
Add the following sentence:
Suitable test organisms are described in Annex K.
6.6.2, after the second sentence
Add the following sentence:
Suitable test organisms are described in Annex K.

Annex K
Add the following text as a new annex.

ISO 11133:2014/Amd.2:2020(E)
Annex K
(normative)
Performance testing of confirmation media and reagents
This annex specifies control strains for the performance testing of confirmation and characterization
media, reagents, dyes, stains and materials described in standards for the microbiological examination
of samples from the food chain and water.
For the microbiological media and reagents under test, the inoculum used is a subculture of an isolated
colony. Therefore, the method of performance testing for these products is qualitative.
The shortest permissible incubation time specified in the relevant International Standard for the
confirmation or characterization test should be used for the positive control organism(s), while the
longest permissible incubation time should be used for the negative control organism(s).
The strains chosen in Table K.1 have been selected preferentially from those already cited in this
document. If a suitable strain was not available from this source, a strain from the catalogue of
[20]
organisms compiled by the World Data Centre for Microorganisms (WDCM) has been selected.
In most cases, more than one control strain has been listed in Table K.1 for both positive and negative
reactions. The user may choose any of the strains cited for positive and negative reactions.
If control strains for performance testing of confirmation or characterization media, reagents, dyes,
stains and materials are already specified in the International Standard, for example, as in ISO 10272-1
and ISO 10272-2 (Campylobacter) and ISO 10273 (Yersinia enterocolitica), they have not been included in
Table K.1. In addition, serological reagents have not been included.
If commercially sourced media or reagents are used, follow the manufacturer’s instructions, including
time, temperature and conditions of performance. If the instructions do not include control strains,
choose a positive and a negative strain from Table K.1. See Clause 6 for requirements.
2 © ISO 2020 – All rights reserved

ISO 11133:2014/Amd.2:2020(E)
Table K.1 — Control strains for confirmation and characterization media, reagents, dyes,
stains and materials included in documents
from ISO/TC 34/SC 9, ISO/TC 34/SC 5 and ISO/TC 147/SC 4
b
International WDCM Characteristic
a
Medium/reagent Function Control strains
Standard numbers reactions
Positive reaction:
Pseudomonas aeruginosa 00025
Yellow to brick red after adding
1 to 2 drops of Nessler’s reagent
Acetamide broth Detection of ammonia
with Nessler’s ISO 16266 production from 00012
reagent acetamide
00013 Negative reaction:
Escherichia coli
00090 No yellow to brick red colour
Positive reaction:
Escherichia coli
Blue colonies with surrounding
Acetate agar medium blue/green
Growth on acetate
ISO 21567
(Sodium acetate
agar
agar)
Shigella sonnei 00127 Negative reaction:
No growth or very weak growth,
Shigella flexneri 00125 no colour change of the medium
(remains green)
Positive reaction:
Clostridium perfringens 00080
Mauve/purple/violet colour
Acid phosphatase Detection of acid
ISO 14189 00174
reagent phosphatase
Negative reaction:
Clostridium bifermentans 00079
No mauve/purple/violet colour
Positive reaction:
Vibrio fluvialis 00137
Turbidity and violet/purple
Arginine
Detection of L-Arginine
colour
dihydrolase saline ISO 21872-1
dihydrolase
medium
00037 Negative reaction:
Vibrio parahaemolyticus
00185 Yellow colour
Enterococcus faecalis
Positive reaction:
Tan to black colour in the
surrounding medium
Enterococcus faecium
Bile aesculin azide Detection of aesculin
ISO 7899-2
agar hydrolysis
Aerococcus viridans 00061
Negative reaction:
No tan to black colour in the
Escherichia coli surrounding medium
a
Strain free of choice; one of the strains has to be used as a minimum.
b [20]
Refer to the reference strain catalogue available on http:// www .wfcc .info for information on culture collection strain numbers and contact details .
c
Some national restrictions and directions can require the use of a different serovar. Refer to national requirements relating to the choice of Salmonella
serovars.
d
Weak coagulase-producing strain of S. aureus.
SIST EN ISO 11133:
...

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