SIST EN 47:2005

Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)

This document specifies a method for the determination of the toxic values of a wood preservative against the larvae of Hylotrupes bajulus (Linnaeus), introduced into wood treated previously by full impregnation.
This method is applicable to:
3   water-insoluble chemicals which are being studied as active insecticides;
3   organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
3   organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates;
3   water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.

Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Larven von Hylotrupes bajulus (Linnaeus) - (Laboratoriumsverfahren)

Dieses Dokument legt ein Verfahren zur Bestimmung der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Larven von Hylotrupes bajulus (Linnaeus) fest, die in Holz eingesetzt wurden, das zuvor durch Volltränkung behandelt wurde.
Das Verfahren gilt für:
-   wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
-   organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
-   organische, in Wasser dispergierbare Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
-   wasserlösliche Stoffe, zum Beispiel Salze.
Das Verfahren gilt unabhängig davon, ob die Proben einer angemessenen Alterungsbeanspruchung ausgesetzt wurden oder nicht.

Produits de préservation du bois - Détermination du seuil d'efficacité contre les larves d'Hylotrupes bajulus (Linnaeus) - (Méthode de laboratoire)

La présente Norme européenne prescrit une méthode de détermination du seuil d'efficacité d'un produit de préservation du bois contre les larves d'Hylotrupes bajulus (Linnaeus) apres leur introduction dans du bois totalement imprégné.
La présente méthode est applicable :
3 aux produits chimiques non hydrosolubles étudiés en tant que matieres actives insecticides, ou
3 aux formules organiques telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés, ou
3 aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés, ou
3 aux produits solubles dans l'eau, par exemple aux sels.
Cette méthode s'applique a des éprouvettes d'essai ayant subi ou non des épreuves de vieillissement appropriées.

Zaščitna sredstva za les - Ugotavljanje toksičnih vrednosti proti ličinkam hišnega kozlička Hylotrupes bajulus (Linnaeus) (Laboratorijska metoda)

General Information

• Status: Withdrawn
• Publication Date: 30-Jun-2005
• Withdrawal Date: 12-Dec-2016
• ICS: 71.100.50 - Wood-protecting chemicals
• Technical Committee: LLZ - Les, lesni izdelki in zaščita lesa
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 13-Dec-2016
• Due Date: 05-Jan-2017
• Completion Date: 13-Dec-2016
• Directive: 89/106/EEC - Construction products
• Ref Project: EN 47:2005 - Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)

Relations

• Replaced By: SIST EN 47:2017 - Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)
• Effective Date: 01-Jan-2017
• Corrected By: SIST EN 47:2005/AC:2007 - Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)
• Effective Date: 08-Jun-2022
• Corrected By: SIST EN 47:2005/AC:2007 - Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)
• Effective Date: 01-Dec-2007
• Replaces: SIST EN 47:1996 - Wood preservatives - Determination of the toxic values against Hylotrupes bajulus (Linnaeus) larvae (Laboratory method)
• Effective Date: 01-Jul-2005
• Replaces: SIST EN 47:1996/AC:2002 - Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) (Laboratory method)
• Effective Date: 01-Jul-2005

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 47:2005
01-julij-2005
1DGRPHãþD
SIST EN 47:1996
SIST EN 47:1996/AC:2002
=DãþLWQDVUHGVWYD]DOHV8JRWDYOMDQMHWRNVLþQLKYUHGQRVWLSURWLOLþLQNDPKLãQHJD
NR]OLþND+\ORWUXSHVEDMXOXV/LQQDHXV/DERUDWRULMVNDPHWRGD
Wood preservatives - Determination of the toxic values against larvae of Hylotrupes
bajulus (Linnaeus) - (Laboratory method)
Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Larven von
Hylotrupes bajulus (Linnaeus) - (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination du seuil d'efficacité contre les larves
d'Hylotrupes bajulus (Linnaeus) - (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 47:2005
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST EN 47:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN 47:2005

---------------------- Page: 2 ----------------------

SIST EN 47:2005



EUROPEAN STANDARD
EN 47

NORME EUROPÉENNE

EUROPÄISCHE NORM
March 2005
ICS 71.100.50 Supersedes EN 47:1988
English version
Wood preservatives - Determination of the toxic values against
larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)
Produits de préservation des bois - Détermination des Holzschutzmittel - Bestimmung der Grenze der
valeurs toxiques contre les larves d'Hylotrupes bajulus Wirksamkeit gegenüber Larven von Hylotrupes bajulus
(Linnaeus) - (Méthode de laboratoire) (Linnaeus) - (Laborverfahren)
This European Standard was approved by CEN on 3 February 2005.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.

CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.




EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 47:2005: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative reference .5
3 Terms and definitions .5
4 Principle.5
5 Test materials.6
6 Sampling.8
7 Test specimens.9
8 Procedure .10
9 Expression of results .13
10 Test report .14
Annex A (informative) Example of a test report .16
Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) .18
Annex C (normative) Differentiation of heartwood and sapwood in Pinus species.21
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory .22
Bibliography .23

2

---------------------- Page: 4 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
Foreword
This document (EN 47:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and
wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by September 2005, and conflicting national standards shall be
withdrawn at the latest by September 2005.
This document supersedes EN 47:1988.
Significant technical differences between this document and EN 47:1988 are as follows:
a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests;
b) acknowledgement of the terms given in EN 1001-1;
c) introduction of an informative Annex to take account of consideration for minimisation of environmental
and health hazards caused by the use of this biological test.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.

3

---------------------- Page: 5 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
Introduction
This document describes a laboratory method of testing which gives a basis for the general assessment of the
effectiveness of a wood preservative against Hylotrupes bajulus by determination and comparison with
different classes of larvae, of the concentration at which the product prevents their survival in totally
impregnated wood of a susceptible species.
In this respect it differs from the method specified in EN 46-1 which is intended to determine whether a
preservative applied to the surface is capable of preventing infestation of wood by these larvae.
This laboratory method provides a criterion by which the value of a preservative can be assessed. In making
this assessment the methods by which the preservative may be applied should be taken into account. It is
further recommended that results from this test should be supplemented by those from other appropriate tests
and, above all, by comparison with practical experience.
When products which are very active at very low concentration are used, it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other products,
treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate
rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see
also Annex E for environmental, health and safety precautions).

4

---------------------- Page: 6 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
1 Scope
This document specifies a method for the determination of the toxic values of a wood preservative against the
larvae of Hylotrupes bajulus (Linnaeus), introduced into wood treated previously by full impregnation.
This method is applicable to:
 water-insoluble chemicals which are being studied as active insecticides;
 organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
 organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates; and
 water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing
procedures.
2 Normative reference
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the
total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
Impregnation of several sets of test specimens of susceptible wood species with a series of concentrations of
the preservative.
Introduction of Hylotrupes bajulus larvae of a given category into these test specimens and determination of
their survival rate at fixed intervals of time.
Comparison of the results with those obtained with untreated and solvent or diluent-treated control test
specimens. Derivation of the toxic values of the product under test for the category of larvae in question.
5

---------------------- Page: 7 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
5 Test materials
5.1 Biological material
5.1.1 Hylotrupes bajulus (Linnaeus) larvae
 Category 1 (obligatory test): larvae within a maximum of 3 days of hatching.
 Category 2 (optional additional test): larvae with masses in the range 50 mg to 150 mg.
5.1.2 Source of larvae
The larvae shall preferably be obtained from cultures reared according to the method described in Annex B.
NOTE Otherwise larvae in Category 2 can be taken from naturally infested wood, in which case they should be
transferred into sapwood of pine and stored for at least 4 weeks under the rearing conditions specified in Annex B.
Do not use the larvae in the test if they have not fed normally during this storage period.
5.1.3 Provision of larvae
Collect larvae in Category 1 from eggs laid by different females.
Carefully cut out the larvae in Category 2 from the culture blocks and keep them separated from one another
for 2 days to 3 days in the culturing chamber (5.3.1) to check that they are healthy.
5.1.4 Choice of larvae in Category 2
Use only healthy larvae in the test.
NOTE A healthy larva can be recognized by its ivory-white colour, its firm consistency and rounded appearance, and
by the absence of wounds or bites which show up as dark marks. Healthy larvae react to the touch by vigorous movement
and attempts to bite.
Reject any larvae which are shrunken or aged, which have recently moulted, or which are in a pre-pupal stage.
5.1.5 Number of larvae
The number of larvae per treated and control test specimen shall be six of Category 1 or one of Category 2.
Sort the larvae retained in Category 2 mentioned above.
Do not use larvae weighing more than 150 mg as they may pupate and therefore interfere with the test.
For a single test, use a mixed batch of larvae of Category 1 and for Category 2, as far as possible, use larvae
of similar masses. The number of larvae necessary is given in Table 1.

6

---------------------- Page: 8 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
Table 1 – Number of larvae and test specimens
Larvae in Category 2
Concentrations Larvae in Category 1
a
Without radiography
With radiography
of
Type of test
preservatives
specimen
Number of Number of Number of
Number Number Number
test test test
Mass fraction
of larvae of larvae of larvae
specimens specimens specimens
Treated test
1 5 30 10 10 7 7
specimens
_ 2 5 30 10 10 7 7
_ 3 5 30 10 10 7 7
_ 4 5 30 10 10 7 7
_ 5 5 30 10 10 7 7
etc.
Untreated control test 0 5 30 10 10 7 7
specimens
Solvent or diluent 0 5 30 10 10 7 7
control test specimens
(including water)
Total for 5 concentrations 35 210 70 70 49 49
a
The use of radiography is only recommended in the case of tests with larvae in Category 2.


5.2 Products and reagents
5.2.1 Xylene, technical grade, mixed isomers.
5.2.2 Water, complying with grade 3 of EN ISO 3696.
5.2.3 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a
residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.4 Cellulose or absorbent cotton wool and filter paper
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, and controlled at (28 ± 2) °C and at relative humidity
(85 ± 5) %.
5.3.2 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and at relative humidity
(65 ± 5) %.
NOTE The conditioning of test specimens can be carried out in the laboratory work area (see 5.3.3) provided that this
has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
7

---------------------- Page: 9 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their vapours.
5.3.4 Testing chamber, ventilated and air-conditioned, controlled at (22 ± 2) °C and at a relative humidity
of (70 ± 5) %.
5.3.5 Treatment vessels, of a material that does not react with the preservative under test, for example of
glass for organic products and of polyethylene for salts containing fluorine.
5.3.6 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with
which they come into contact during the test.
5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to
ensure the safety of the operator.
5.3.8 Vacuum vessel(s), fitted with stopcocks, capable of receiving the treatment vessels (5.3.5).
1)
5.3.9 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa .
5.3.10 Drying vessel(s), capable of holding sets of five test specimens (7.5), provided with a close-fitting
cover and containing supports that will give minimum contact with treated test specimens to be placed on
them. The vessels and supports shall be of a material that does not react with the preservative under test, for
example glass for organic compounds and polyethylene for products containing fluorine.
5.3.11 Drill and twist drills, approximately 3,0 mm to 4,5 mm in diameter, and a fine awl. In all cases, the
number of bits shall be sufficient to drill holes to the size of the larvae available; in the case of larvae of
Category 1, use a steel awl.
5.3.12 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.
5.3.13 X-ray apparatus (optional) with tungsten target and beryllium window, with voltage and current
continuously variable in the following ranges:
 voltages: 10 kV to 50 kV;
 current: 0 mA to 15 mA.
5.3.14 Protective gloves
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored and
handled in accordance with any written recommendations from the supplier.
NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.


1) 100 Pa = 1 mbar.
8

---------------------- Page: 10 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
7 Test specimens
7.1 Species of wood
2)
The reference species is Scots pine (Pinus sylvestris Linnaeus) .
NOTE Additional tests may be carried out using other species but, if so, this should be stated in the test report.
7.2 Wood quality
The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not
have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees
preferably felled in winter. The wood shall not have been stored for more than five years.
NOTE 1 Wood that has been kiln dried at temperatures below 60 °C may be used.
The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm
and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the
whole.
NOTE 2 It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimens
Prepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum of 2 mm
from any faces exposed during drying. The longitudinal faces shall be parallel to the direction of the grain. The
annual rings shall have a contact angle of greater than 10° to the faces of the test specimens. Make
transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give test
specimens (50 ± 0,5) mm long.
The specimens shall originate from a minimum of three trees or shall be taken at random from a stock
originally of more than 500 test specimens.
7.4 Dimensions of test specimens
The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be
(50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.
For the purposes of calculating the mass of preservative retained per unit volume of wood (8.1.2.2) the
3
nominal volume of each test specimen shall be taken as 18,75 cm .
Mark each test specimen so that it can be identified throughout the test.
7.5 Number of test specimens
The number of test specimens required is given in Table 1.
It is advisable to treat more than the specified number of test specimens so that, after weighing, any test
specimens with abnormally high or low retentions can be rejected from the batch.

2) In southern European countries the species of pine most frequently infested by Hylotrupes bajulus may be used as an
alternative, provided that the suitability of the species for use in the tests specified in this document has been
demonstrated in all aspects (development of larvae, resistance to impregnation, etc.).
9

---------------------- Page: 11 ----------------------

SIST EN 47:2005
EN 47:2005 (E)
8 Procedure
8.1 Preparation of test specimens
8.1.1 Conditioning of test specimens before treatment
Allow the test specimens to condition in the conditioning chamber (5.3.2), for a minimum of two weeks.
8.1.2 Treatment of the test specimens
8.1.2.1 Preparation of the treatment solutions
8.1.2.1.1 Solid preservatives
Water-soluble preservatives :
 dissolve the preservative in the water (5.2.2) in a series of concentrations.
Non-water-soluble preservatives:
 dissolve the preservative in an appropriate solvent (5.2.3) in a series of concentrations.
8.1.2.1.2 Liquid preservatives
If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a
concentrate, dilute the preservative with the diluent to the required working concentration, using the procedure
specified by the manufacturer.
All treatment solutions shall be freshly prepared.
8.1.2.1.3 Preparation
Prepare a series of at least five concentrations by mass, distributed evenly about the expected toxic values. A
solvent or diluent control, i.e. treatment at concentration = 0, shall also be used. If the approximate toxic
values are unknown, the concentrations shall form a widely spaced geometric progression for a first test and a
more closely spaced geometric or arithmetic progression for subsequent tests.
All treatment solutions shall be freshly prepared.
8.1.2.2 Impregnation
Carry out impregnation in ascending order of concentration, starting with the solvent control (concentration =
0).
The following procedure ensures the required complete impregnation of test specimens by the test solutions.
For each concentration weigh each test specimen, to the nearest 0,05 g, and then stack the test specimens in
one of the treatment vessels (5.3.5) so that as much of their surface as possible is exposed (e.g. by piling
them crosswise). Ballast the stack of test specimens with the weights (5.3.6) to prevent them from floating
later when the liquid is admitted.
Place each beaker in one of the vacuum vessels (5.3.8), attach the vacuum pump (5.3.9) and reduce the
pressure to 700 Pa. Maintain it for 15 min. Observe the proper safety measures for vacuum vessels. After this
period, close the stopcock to the vacuum pump (5.3.9) and open the other stopcock to al
...