SIST-TS CEN/TS 12404:2015
(Main)Durability of wood and wood-based products - Assessment of the effectiveness of a masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Laboratory method
Durability of wood and wood-based products - Assessment of the effectiveness of a masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Laboratory method
This European prestandard specifies a method for determining the performance of a preservative, applied to the upper surface of the mortar test specimens, in preventing the growth of dry rot through the treated mortar when exposed to the tests fungus. This method is only applicable to masonry fungicides applied as a true solution of the preservative in water or as a water-borne liquid emulsion-type preservative.
Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der Wirksamkeit eines Schutzmittels gegen das Überwachsen von Echtem Hausschwamm Serpula lacrymans (Schumacher ex Fries) S.F. Gray vom Mauerwerk auf das Holz - Laboratoriumsverfahren
Diese Europäische Norm legt ein Verfahren zur Bestimmung der Wirksamkeit eines Schutzmittels zur Verhinderung des Durchwachsens des „Echten Hausschwamms" durch den behandelten Mörtel, bei dem auf ihrer Oberseite behandelte Mörtelprüfkörper der Einwirkung des Prüfpilzes ausgesetzt werden, fest.
Dieses Verfahren ist nur für Mauerwerksfungizide anwendbar, die als echte Lösung des Schutzmittels in Wasser oder als wasserbasierte Schutzmittel-Emulsionen angewendet werden. Es gilt nicht für Schutzmittelpatronen, Pasten und ähnliche Schutzmitteltypen. Das Verfahren ist anwendbar für Schutzmittel, die mittels Streich-, Sprüh,- und/oder Injektionstechniken auf das Mauerwerk aufgebracht oder Putz- und Gipsverputzmörteln für Mauerwerke zugemischt werden.
Durabilité du bois et des matériaux dérivés du bois - Évaluation de l'efficacité d'un fongicide de maçonnerie pour empêcher le développement dans le bois de la mérule Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Méthode de laboratoire
La présente Norme européenne décrit une méthode de détermination de la performance d'un produit de préservation, appliqué sur la surface supérieure d'éprouvettes de mortier, en vue d'empêcher le développement de la mérule au travers du mortier traité après exposition au champignon d'essai.
La présente méthode est applicable seulement aux fongicides pour maçonneries appliqués en tant que solution aqueuse de produit de préservation ou en tant qu'émulsion de produit de préservation en phase aqueuse. Elle n'est pas applicable aux produits de préservation à l'état de solide, de pâte ou équivalent. Cette méthode est applicable aux produits de préservation appliqués à la maçonnerie par badigeonnage, par des procédés de pulvérisation et/ou d'injection ou mélangés à des enduits pour maçonnerie applicables en extérieur et/ou en intérieur.
Trajnost lesa in lesnih izdelkov - Ocena učinkovitosti sredstva za zaščito gradbenega materiala na preprečevanje širjenja sive hišne gobe Serpula lacrymans (Schumacher ex Fries ) S.F. Gray na les - Laboratorijska metoda
Ta evropski standard določa metodo za ugotavljanje učinkovitosti zaščitnega sredstva, nanesenega na zgornjo površino preskušancev malte, pri preprečevanju širjenja hišne gobe skozi obdelano malto pri izpostavljenosti preskusnim glivam. Ta metoda se uporablja samo za sredstva za zaščito gradbenega materiala, ki so nanesena kot prava raztopina zaščitnega sredstva v vodi ali kot tekoče emulzijsko zaščitno sredstvo na vodni osnovi.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST-TS CEN/TS 12404:2015
01-maj-2015
1DGRPHãþD
SIST-TS ENV 12404:2004
7UDMQRVWOHVDLQOHVQLKL]GHONRY2FHQDXþLQNRYLWRVWLVUHGVWYD]D]DãþLWR
JUDGEHQHJDPDWHULDODQDSUHSUHþHYDQMHãLUMHQMDVLYHKLãQHJREH6HUSXOD
ODFU\PDQV 6FKXPDFKHUH[)ULHV 6)*UD\QDOHV/DERUDWRULMVNDPHWRGD
Durability of wood and wood-based products - Assessment of the effectiveness of a
masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans
(Schumacher ex Fries) S.F. Gray - Laboratory method
Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der Wirksamkeit eines
Schutzmittels gegen das Überwachsen von Echtem Hausschwamm Serpula lacrymans
(Schumacher ex Fries) S.F. Gray vom Mauerwerk auf das Holz - Laboratoriumsverfahren
Durabilité du bois et des matériaux dérivés du bois - Évaluation de l'efficacité d'un
fongicide de maçonnerie pour empêcher le développement dans le bois de la mérule
Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Méthode de laboratoire
Ta slovenski standard je istoveten z: CEN/TS 12404:2015
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST-TS CEN/TS 12404:2015 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST-TS CEN/TS 12404:2015
TECHNICAL SPECIFICATION
CEN/TS 12404
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
February 2015
ICS 71.100.50 Supersedes ENV 12404:1997
English Version
Durability of wood and wood-based products - Assessment of
the effectiveness of a masonry fungicide to prevent growth into
wood of Dry Rot Serpula lacrymans (Schumacher ex Fries) S.F.
Gray - Laboratory method
Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung
Évaluation de l'efficacité d'un fongicide de maçonnerie pour der Wirksamkeit eines Schutzmittels gegen das
empêcher le développement dans le bois de la mérule Überwachsen von Echtem Hausschwamm Serpula
Serpula lacrymans (Schumacher ex Fries) S.F. Gray - lacrymans (Schumacher ex Fries) S.F. Gray vom
Méthode de laboratoire Mauerwerk auf das Holz - Laboratoriumsverfahren
This Technical Specification (CEN/TS) was approved by CEN on 6 October 2014 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 12404:2015 E
worldwide for CEN national Members.
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Contents Page
Foreword .3
Introduction .4
1 Scope .5
2 Normative references .5
3 Terms and definitions .5
4 Principle .5
5 Test materials .6
5.1 Test fungus .6
5.1.1 Obligatory test fungus .6
5.1.2 Optional test fungi .6
5.1.3 Maintenance of strains .6
5.2 Products and reagents .6
5.2.1 Water, distilled or deionized, conform to grade 3 of EN ISO 3696. .6
5.2.2 Malt – mineral salt – agar culture medium; consisting:.6
5.3 Apparatus .7
5.3.1 Culture vessels .7
6 Sample of the preservative .8
7 Mortar test specimen .9
7.1 Preparation of mortar .9
7.2 Preparation of mortar test specimen .9
7.3 Curing of mortar test specimen . 11
7.4 Leaching of mortar test specimen . 11
7.5 Number and distribution of mortar test specimens . 11
8 Wood test specimens . 12
8.1 Species of wood. 12
8.2 Quality of wood . 12
8.3 Provision of wood test specimens. 12
8.4 Dimensions of wood test specimens. 12
9 Procedure . 12
9.1 Culturing the test fungus . 12
9.2 Treatment of mortar test specimens. 12
9.3 Preparation of wood test specimens . 12
9.4 Sterilization procedures . 13
9.5 Exposure to fungus . 13
9.6 Examination of the mortar test specimens . 13
9.7 Validity of test . 14
10 Statement of the results . 15
11 Test report . 15
Annex A (informative) Test fungi . 16
Annex B (informative) Methods of sterilization . 18
Annex C (informative) Example of a test report . 20
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Foreword
This document (CEN/TS 12404:2015) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes ENV 12404:1997.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Croatia, Cyprus,
Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany,
Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
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Introduction
This Technical Specification describes a laboratory method of test for the assessment of the effectiveness of a
masonry fungicide applied to masonry for the prevention of the growth of dry rot, Serpula lacrymans
(Schumacher ex Fries) S.F. Gray into wood.
This laboratory method enables the determination of the concentration of a preservative within mortar which
could prevent the dry rot fungus from growing through a given mortar layer treated with this preservative.
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1 Scope
This Technical Specification specifies a method for determining the performance of a preservative, applied to
the upper surface of the mortar test specimens, in preventing the growth of dry rot through the treated mortar
when exposed to the test fungus.
This method is only applicable to masonry fungicides applied as a true solution of the preservative in water or
dilute oil in water emulsion. It is not applicable to rods, pastes and other similar preservative types. This
method is applicable to preservatives applied to masonry by brushing, spraying and/or injection techniques or
mixed into rendering and plastering mortar for masonry.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
EN 113:1996, Wood preservatives - Test method for determining the protective effectiveness against wood
destroying basidiomycetes - Determination of the toxic values
EN 413-1, Masonry cement - Part 1: Composition, specifications and conformity criteria
EN 459-1, Building lime - Part 1: Definitions, specifications and conformity criteria
EN 599-1, Durability of wood and wood-based products - Efficacy of preventive wood preservatives as
determined by biological tests - Part 1: Specification according to use class
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
masonry fungicide
fungicidal/fungistatic product applied to masonry and other mineral construction materials to prevent the
growth of dry rot through or over the treated material
3.2
performance
behaviour of the preservative product in terms of its effectiveness in test
3.3
preservative
formulated masonry fungicide in the form received from the supplier for the test
3.4
supplier
sponsor of the test
4 Principle
The preservative to be tested is applied by pipette (or in accordance with the sponsor's instruction) to the
upper surface of mortar test specimens. The mortar test specimens are contained in rigid tubes and an
untreated wooden sample is placed on top of these mortar test specimens. The bases of the mortar
specimens are exposed to dry rot attack for a given time. The assessment of the performance of the test
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preservative consists of checking the growth of the fungus through the mortar and the evaluation of any attack
of the wooden sample contained in the rigid tube.
5 Test materials
5.1 Test fungus
5.1.1 Obligatory test fungus
— Serpula lacrymans (Schumacher ex Fries) S.F. Gray, strain BAM Ebw.315.
5.1.2 Optional test fungi
For specific regional uses or conditions, it is also possible to use other strains of dry rot (e.g.Serpula
lacrymans FPRL 12 C) known to be capable of growing through masonry.
NOTE Other fungal species can grow through masonry. This method of test could be used to assess the ability of
these fungi to grow through mortar specimens.
5.1.3 Maintenance of strains
The strains shall be maintained and treated in accordance with the instructions from their laboratory of origin
(see annex A). If a strain shows signs of degeneration, it shall no longer be used and the testing laboratory
shall obtain a new standard culture of the strain.
5.2 Products and reagents
5.2.1 Water, distilled or deionized, conform to grade 3 of EN ISO 3696.
5.2.2 Malt – mineral salt – agar culture medium; consisting:
— malt extract in concentrated form 12,50 g
or in powder form 10,00 g
— agar causing no inhibition of growth of fungi 15,00 g
— potassium dihydrogen phosphate (KH2PO4) 2,72 g
— calcium sulfate dihydrate (CaSO4.2H2O) 0,38 g
— magnesium sulfate heptahydrate (MgSO4.7H2O) 0,62 g
— water (5.2.1) to make up to 1 000 ml.
Place all the ingredients in a 1 000 ml beaker measure and gently heat, stirring occasionally, until completely
dissolved.
Pour 150 ml of the culture medium into each culture vessel (5.3.1).
Close the vessels with screw cap without a hole a quarter of a turn less than full closure and sterilise the
closed vessels in the autoclave (5.3.8) at (121 ± 2) °C for 30 min. Let them cool standing upright.
5.2.3 Nutrient solution, a mass fraction for 5 % aqueous solution of malt extract.
5.2.4 Equipment for chemical gas or for steam sterilisation or access to a radiation source (see
Annex B).
5.2.5 Carbon dioxide, compressed gas in cylinders.
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5.2.6 Sodium chloride, saturated solution in water.
5.2.7 Portland cement, conforming to EN 413-1.
5.2.8 Hydrated building lime, conforming to EN 459-1.
5.2.9 Bricklaying mortar sand, quartz sand with a particle size equal to or less than 1 mm, washed under
running tap water until the water is no longer turbid.
5.3 Apparatus
5.3.1 Culture vessels
Straight sided flat bottom glass culture vessels with an aperture of 50 mm to 60 mm (see Figure 3), provided
with both screw caps without a hole, used for culturing the test fungus (9.1), and screw caps with a central
hole equal in size to the outer diameter of the tube (5.3.2.) plus the thickness of the tubing (5.3.3.) in diameter.
NOTE The alternative type C.2 of test vessels described in EN 113, have been found to be suitable.
5.3.2 Rigid tubes, which can be sterilized using an autoclave (for example glass, or polyvinylidene fluoride)
with an inner diameter of 35 mm to 46 mm and a length of at least 150 mm.
5.3.3 Tubing, with a diameter corresponding to the outer diameter of the rigid tubes (5.3.2.) with a wall
thickness of (1,0 ± 0,5) mm and cut into lengths of (40,0 ± 1,0) mm capable of being sterilized using an
autoclave.
NOTE Tubing made of rubber has been found to be suitable.
5.3.4 Inert supports of maximum thickness 3 mm and when in use, do not obscure more than 10 % of the
mortar surface.
NOTE Stainless steel washers of overall diameter 25 mm have been found to be suitable. Two supports are required
for each test assembly.
5.3.5 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.6 Culture chamber, dark and controlled at (22 ± 1) °C and (70 ± 5) % relative humidity.
5.3.7 Drying oven, capable of being controlled at (45 ± 1) °C.
5.3.8 Autoclave, adjustable to (121 ± 2) °C.
5.3.9 Containers, to prepare the mortar and the preservative solutions, made of a material that does not
react with their contents.
5.3.10 Mortar mould apparatus, consisting of a plastic frame for example polyvinyl chloride (PVC) with a
height of (10 ± 0,5) mm, a porous support (for example clay house building bricks, ceramic plates) to absorb
the excess water in the fresh mortar and a cloth (for example muslin cloth, cheese cloth) with the same
dimensions as the frame to aid demoulding the mortar specimens (see Figure 2).
5.3.11 Plastic lath, used to smooth the surface of the mortar after casting in the plastic frame.
5.3.12 Circular tamper with a flat base, a diameter of 3 mm to 5 mm to less than the internal diameter of the
rigid tube (5.3.2), and at least 50 mm longer than the rigid tube.
5.3.13 Racks on which to place the treated mortar test specimens an example is shown in Figure 1.
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Dimensions in millimetres
Key
1 wad of cotton wool
2 tubing
3 culture vessel screw cap
4 rigid tube
5 mortar specimen
Figure 1 — Example of a rack
5.3.14 Sterile single-use pipettes of (1,0 ± 0,1) ml content.
5.3.15 Ordinary laboratory equipment, including for example balances accurate to 0,01 g, sealable
containers, forceps.
5.3.16 Microbiological safety cabinet, providing protection to the operator and to the work.
6 Sample of the preservative
The sample shall be representative of the product to be tested. It shall be identified as specified in EN 599-1.
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7 Mortar test specimen
7.1 Preparation of mortar
Dry a quantity of the quartz sand (5.2.9) in the drying oven (5.3.7) at (45 ± 1) °C. Measure out nine parts by
volume of the dried quartz sand and weigh, then place into a container (5.3.9). Add two parts by volume
hydrated building lime (5.2.8) and one part by volume Portland cement (5.2.7) and mix thoroughly. Add 16 ml
water (5.2.1) per 100 g quartz sand and mix until a homogeneous mortar is obtained.
7.2 Preparation of
...
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