SIST EN 12404:2020
(Main)Durability of wood and wood-based products - Assessment of the effectiveness of masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Laboratory method
Durability of wood and wood-based products - Assessment of the effectiveness of masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Laboratory method
This document specifies a method for determining the performance of a preservative, applied to the upper surface of the mortar test specimens, in preventing the growth of dry rot through the treated mortar when exposed to the test fungus.
This method is only applicable to masonry fungicides applied as a true solution of the preservative in water or dilute oil in water emulsion. It is not applicable to rods, pastes and other similar preservative types. This method is applicable to preservatives applied to masonry by brushing, spraying and/or injection techniques or mixed into rendering and plastering mortar for masonry.
Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der Wirksamkeit eines Schutzmittels gegen das Überwachsen von Echtem Hausschwamm Serpula lacrymans (Schumacher ex Fries) S.F. Gray vom Mauerwerk auf das Holz - Laboratoriumsverfahren
Dieses Dokument legt ein Verfahren zur Bestimmung der Wirksamkeit eines Schutzmittels zur Verhinderung des Durchwachsens des Echten Hausschwamms durch den behandelten Mörtel, bei dem auf ihrer Oberseite behandelte Mörtelprüfkörper der Einwirkung des Prüfpilzes ausgesetzt werden, fest.
Dieses Verfahren ist nur für Mauerwerksfungizide anwendbar, die als echte Lösung des Schutzmittels in Wasser oder als wasserbasierte Schutzmittel-Emulsionen angewendet werden. Es gilt nicht für Schutzmittelpatronen, Pasten und ähnliche Schutzmitteltypen. Das Verfahren ist anwendbar für Schutzmittel, die mittels Streich-, Sprüh,- und/oder Injektionstechniken auf das Mauerwerk aufgebracht oder Putz- und Gipsverputzmörteln für Mauerwerke zugemischt werden.
Durabilité du bois et des matériaux dérivés du bois - Évaluation de l'efficacité d'un fongicide de maçonnerie pour empêcher le développement dans le bois de la mérule Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Méthode de laboratoire
Le présent document décrit une méthode de détermination de la performance d'un produit de préservation, appliqué sur la surface supérieure d'éprouvettes de mortier, en vue d'empêcher le développement de la mérule au travers du mortier traité après exposition au champignon d'essai.
La présente méthode est applicable seulement aux fongicides pour maçonneries appliqués en tant que solution aqueuse de produit de préservation ou en tant qu'émulsion de produit de préservation en phase aqueuse. Elle n'est pas applicable aux produits de préservation à l'état de solide, de pâte ou équivalent. Cette méthode est applicable aux produits de préservation appliqués à la maçonnerie par badigeonnage, par des procédés de pulvérisation et/ou d'injection ou mélangés à des enduits pour maçonnerie applicables en extérieur et/ou en intérieur.
Trajnost lesa in lesnih proizvodov - Ocena učinkovitosti biocidnih proizovodov za zaščito gradbenega materiala pred širjenjem sive hišne gobe Serpula lacrymans (Schumacher ex Fries ) S.F. Gray na les - Laboratorijska metoda
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN 12404:2020
01-julij-2020
Nadomešča:
SIST-TS CEN/TS 12404:2015
Trajnost lesa in lesnih izdelkov - Ocena učinkovitosti sredstva za zaščito
gradbenega materiala na preprečevanje širjenja sive hišne gobe Serpula
lacrymans (Schumacher ex Fries ) S.F. Gray na les - Laboratorijska metoda
Durability of wood and wood-based products - Assessment of the effectiveness of
masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans
(Schumacher ex Fries) S.F. Gray - Laboratory method
Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der Wirksamkeit eines
Schutzmittels gegen das Überwachsen von Echtem Hausschwamm Serpula lacrymans
(Schumacher ex Fries) S.F. Gray vom Mauerwerk auf das Holz - Laboratoriumsverfahren
Durabilité du bois et des matériaux dérivés du bois - Évaluation de l'efficacité d'un
fongicide de maçonnerie pour empêcher le développement dans le bois de la mérule
Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Méthode de laboratoire
Ta slovenski standard je istoveten z: EN 12404:2020
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
SIST EN 12404:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN 12404:2020
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SIST EN 12404:2020
EN 12404
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2020
EUROPÄISCHE NORM
ICS 71.100.50 Supersedes CEN/TS 12404:2015
English Version
Durability of wood and wood-based products - Assessment
of the effectiveness of masonry fungicide to prevent
growth into wood of Dry Rot Serpula lacrymans
(Schumacher ex Fries) S.F. Gray - Laboratory method
Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten -
Évaluation de l'efficacité d'un fongicide de maçonnerie Bestimmung der Wirksamkeit eines Schutzmittels
pour empêcher le développement dans le bois de la gegen das Überwachsen von Echtem Hausschwamm
mérule Serpula lacrymans (Schumacher ex Fries) S.F. Serpula lacrymans (Schumacher ex Fries) S.F. Gray
Gray - Méthode de laboratoire vom Mauerwerk auf das Holz -
Laboratoriumsverfahren
This European Standard was approved by CEN on 20 April 2020.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 12404:2020 E
worldwide for CEN national Members.
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SIST EN 12404:2020
EN 12404:2020 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Test materials . 7
5.1 Test fungus . 7
5.1.1 Obligatory test fungus . 7
5.1.2 Optional test fungi . 7
5.1.3 Maintenance of strains . 7
5.2 Products and reagents . 7
5.3 Apparatus . 8
6 Sample of the preservative . 10
7 Mortar test specimen . 10
7.1 Preparation of mortar . 10
7.2 Preparation of mortar specimen . 10
7.3 Curing of mortar test specimen . 12
7.4 Leaching of mortar test specimen . 12
7.5 Number and distribution of mortar test specimens . 12
8 Wood test specimens . 12
8.1 Species of wood . 12
8.2 Quality of wood . 12
8.3 Provision of wood test specimens . 13
8.4 Dimensions of wood test specimens . 13
9 Procedure. 13
9.1 Culturing the test fungus . 13
9.2 Treatment of mortar test specimens . 13
9.3 Preparation of wood test specimens . 13
9.4 Sterilization procedures . 13
9.5 Exposure to fungus . 13
9.6 Examination of the mortar test specimens . 14
9.7 Validity of test . 15
10 Statement of the results . 15
11 Test report . 15
Annex A (informative) Test fungi . 16
A.1 General information on maintenance and acquisition of test strains . 16
A.2 Maintenance and treatment of test fungi . 16
A.3 Information regarding obligatory test fungus . 17
2
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EN 12404:2020 (E)
A.4 Information regarding optional test fungus . 17
Annex B (informative) Methods of sterilization . 18
B.1 Ionizing irradiation . 18
B.2 Steam . 18
Annex C (informative) Example of a test report . 19
3
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SIST EN 12404:2020
EN 12404:2020 (E)
European foreword
This document (EN 12404:2020) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2020, and conflicting national standards shall
be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 12404:2015.
In comparison with the previous version, the following technical change has been made:
The list in Subclause 9.6 has been replaced by ratings.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
4
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SIST EN 12404:2020
EN 12404:2020 (E)
Introduction
This document describes a laboratory method of test for the assessment of the effectiveness of a masonry
fungicide applied to masonry for the prevention of the growth of dry rot, Serpula lacrymans (Schumacher
ex Fries) S.F. Gray into wood.
This laboratory method enables the determination of the concentration of a preservative within mortar
which could prevent the dry rot fungus from growing through a given mortar layer treated with this
preservative.
Test results obtained with earlier versions of CEN/TS 12404 are still valid.
5
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SIST EN 12404:2020
EN 12404:2020 (E)
1 Scope
This document specifies a method for determining the performance of a preservative, applied to the
upper surface of the mortar test specimens, in preventing the growth of dry rot through the treated
mortar when exposed to the test fungus.
This method is only applicable to masonry fungicides applied as a true solution of the preservative in
water or dilute oil in water emulsion. It is not applicable to rods, pastes and other similar preservative
types. This method is applicable to preservatives applied to masonry by brushing, spraying and/or
injection techniques or mixed into rendering and plastering mortar for masonry.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 113:1996, Wood preservatives - Test method for determining the protective effectiveness against wood
destroying basidiomycetes - Determination of the toxic values
EN 413-1, Masonry cement - Part 1: Composition, specifications and conformity criteria
EN 459-1, Building lime - Part 1: Definitions, specifications and conformity criteria
EN 599-1, Durability of wood and wood-based products - Efficacy of preventive wood preservatives as
determined by biological tests - Part 1: Specification according to use class
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
masonry fungicide
fungicidal/fungistatic product applied to masonry and other mineral construction materials to prevent
the growth of dry rot through or over the treated material
3.2
performance
behaviour of the preservative product in terms of its effectiveness in test
3.3
preservative
formulated masonry fungicide in the form received from the supplier for the test
3.4
supplier
sponsor of the test
6
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SIST EN 12404:2020
EN 12404:2020 (E)
4 Principle
The preservative to be tested is applied by pipette (or in accordance with the sponsor's instruction) to
the upper surface of mortar test specimens. The mortar test specimens are contained in rigid tubes and
an untreated wooden sample is placed on top of these mortar test specimens. The bases of the mortar
specimens are exposed to dry rot attack for a given time. The assessment of the performance of the test
preservative consists of checking the growth of the fungus through the mortar and the evaluation of any
attack of the wooden sample contained in the rigid tube.
NOTE This method is also valid for other modes of application.
5 Test materials
5.1 Test fungus
5.1.1 Obligatory test fungus
— Serpula lacrymans (Schumacher ex Fries) S.F. Gray, strain BAM Ebw.315.
5.1.2 Optional test fungi
For specific regional uses or conditions, it is also possible to use other strains of dry rot (e.g. Serpula
lacrymans FPRL 12 C) known to be capable of growing through masonry.
NOTE Other fungal species can grow through masonry. This method of test could be used to assess the ability
of these fungi to grow through mortar specimens.
5.1.3 Maintenance of strains
The strains shall be maintained and treated in accordance with the instructions from their laboratory of
origin (see Annex A). If a strain shows signs of degeneration, it shall no longer be used and the testing
laboratory shall obtain a new standard culture of the strain.
5.2 Products and reagents
5.2.1 Water, distilled or deionized, conforming to grade 3 of EN ISO 3696.
5.2.2 Malt – mineral salt – agar culture medium, consisting of:
— malt extract in concentrated form 12,50 g
or in powder form 10,00 g
— agar causing no inhibition of growth of fungi 15,00 g
— potassium dihydrogen phosphate (KH PO ) 2,72 g
2 4
— calcium sulfate dihydrate (CaSO .2H O) 0,38 g
4 2
— magnesium sulfate heptahydrate (MgSO .7H O) 0,62 g
4 2
— water (5.2.1) to make up to 1 000 ml.
Place all the ingredients in a 1 000 ml beaker measure and gently heat, stirring occasionally, until
completely dissolved.
Pour 150 ml of the culture medium into each culture vessel (5.3.1).
Close the vessels with screw cap without a hole a quarter of a turn less than full closure and sterilise the
closed vessels in the autoclave (5.3.8) at (121 ± 2) °C for 30 min. Let them cool standing upright.
7
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SIST EN 12404:2020
EN 12404:2020 (E)
5.2.3 Nutrient solution, a mass fraction for 5 % aqueous solution of malt extract.
5.2.4 Equipment for chemical gas or for steam sterilization or access to a radiation source (see
Annex B).
5.2.5 Carbon dioxide, compressed gas in cylinders.
5.2.6 Sodium chloride, saturated solution in water.
5.2.7 Portland cement, conforming to EN 413-1.
5.2.8 Hydrated building lime, conforming to EN 459-1.
5.2.9 Bricklaying mortar sand, quartz sand with a particle size equal to or less than 1 mm, washed
under running tap water until the water is no longer turbid.
5.3 Apparatus
5.3.1 Culture vessels
Straight sided flat bottom glass culture vessels with an aperture of 50 mm to 60 mm (see Figure 3),
provided with both screw caps without a hole, used for culturing the test fungus (9.1), and screw caps
with a central hole equal in size to the outer diameter of the tube (5.3.2) plus the thickness of the tubing
(5.3.3) in diameter.
NOTE The alternative type C.2 of test vessels described in EN 113, have been found to be suitable.
5.3.2 Rigid tubes, which can be sterilized using an autoclave (for example glass, or polyvinylidene
fluoride) with an inner diameter of 35 mm to 46 mm and a length of at least 150 mm.
5.3.3 Tubing, with a diameter corresponding to the outer diameter of the rigid tubes (5.3.2) with a wall
thickness of (1,0 ± 0,5) mm and cut into lengths of (40,0 ± 1,0) mm capable of being sterilized using an
autoclave.
NOTE Tubing made of rubber has been found to be suitable.
5.3.4 Inert supports of maximum thickness 3 mm and when in use, do not obscure more than 10 % of
the mortar surface.
NOTE Stainless steel washers of overall diameter 25 mm have been found to be suitable. Two supports are
required for each test assembly.
5.3.5 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative
humidity.
5.3.6 Culture chamber, dark and controlled at (22 ± 1) °C and (70 ± 5) % relative humidity.
5.3.7 Drying oven, capable of being controlled at (45 ± 1) °C.
5.3.8 Autoclave, adjustable to (121 ± 2) °C.
5.3.9 Containers, to prepare the mortar and the preservative solutions, made of a material that does
not react with their contents.
8
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SIST EN 12404:2020
EN 12404:2020 (E)
5.3.10 Mortar mould apparatus, consisting of a plastic frame for example polyvinyl chloride (PVC) with
a height of (10 ± 0,5) mm, a porous support (for example clay house building bricks, ceramic plates) to
absorb the excess water in the
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SLOVENSKI STANDARD
oSIST prEN 12404:2019
01-januar-2019
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Durability of wood and wood-based products - Assessment of the effectiveness of a
masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans
(Schumacher ex Fries) S.F. Gray - Laboratory method
Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der Wirksamkeit eines
Schutzmittels gegen das Überwachsen von Echtem Hausschwamm Serpula lacrymans
(Schumacher ex Fries) S.F. Gray vom Mauerwerk auf das Holz - Laboratoriumsverfahren
Durabilité du bois et des matériaux dérivés du bois - Évaluation de l'efficacité d'un
fongicide de maçonnerie pour empêcher le développement dans le bois de la mérule
Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Méthode de laboratoire
Ta slovenski standard je istoveten z: prEN 12404
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
oSIST prEN 12404:2019 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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oSIST prEN 12404:2019
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oSIST prEN 12404:2019
DRAFT
EUROPEAN STANDARD
prEN 12404
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2018
ICS 71.100.50 Will supersede CEN/TS 12404:2015
English Version
Durability of wood and wood-based products - Assessment
of the effectiveness of a masonry fungicide to prevent
growth into wood of Dry Rot Serpula lacrymans
(Schumacher ex Fries) S.F. Gray - Laboratory method
Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten -
Évaluation de l'efficacité d'un fongicide de maçonnerie Bestimmung der Wirksamkeit eines Schutzmittels
pour empêcher le développement dans le bois de la gegen das Überwachsen von Echtem Hausschwamm
mérule Serpula lacrymans (Schumacher ex Fries) S.F. Serpula lacrymans (Schumacher ex Fries) S.F. Gray
Gray - Méthode de laboratoire vom Mauerwerk auf das Holz -
Laboratoriumsverfahren
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 12404:2018 E
worldwide for CEN national Members.
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oSIST prEN 12404:2019
prEN 12404:2018 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Test materials . 7
6 Sample of the preservative . 10
7 Mortar test specimen . 10
7.1 Preparation of mortar . 10
7.2 Preparation of mortar test specimen . 10
7.3 Curing of mortar test specimen . 12
7.4 Leaching of mortar test specimen . 12
7.5 Number and distribution of mortar test specimens . 12
8 Wood test specimens . 12
8.1 Species of wood . 12
8.2 Quality of wood . 12
8.3 Provision of wood test specimens . 13
8.4 Dimensions of wood test specimens . 13
9 Procedure. 13
9.1 Culturing the test fungus . 13
9.2 Treatment of mortar test specimens . 13
9.3 Preparation of wood test specimens . 13
9.4 Sterilization procedures . 13
9.5 Exposure to fungus . 13
9.6 Examination of the mortar test specimens . 14
9.7 Validity of test . 15
10 Statement of the results . 16
11 Test report . 16
Annex A (informative) Test fungi . 17
A.1 General information on maintenance and acquisition of test strains . 17
A.2 Maintenance and treatment of test fungi . 17
A.3 Information regarding obligatory test fungus . 18
A.4 Information regarding optional test fungus . 18
Annex B (informative) Methods of sterilization . 19
B.1 Ionizing irradiation . 19
B.2 Epoxyethane-based sterilant . 19
B.3 Epoxypropane-based sterilant . 19
2
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prEN 12404:2018 (E)
B.4 Water steam . 20
Annex C (informative) Example of a test report . 21
3
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oSIST prEN 12404:2019
prEN 12404:2018 (E)
European foreword
This document (prEN 12404:2018) has been prepared by Technical Committee CEN/TC 38 “Durability
of wood and wood-based products”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN Enquiry.
This document will supersede CEN/TS 12404:2015.
4
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oSIST prEN 12404:2019
prEN 12404:2018 (E)
Introduction
This document describes a laboratory method of test for the assessment of the effectiveness of a masonry
fungicide applied to masonry for the prevention of the growth of dry rot, Serpula lacrymans (Schumacher
ex Fries) S.F. Gray into wood.
This laboratory method enables the determination of the concentration of a preservative within mortar
which could prevent the dry rot fungus from growing through a given mortar layer treated with this
preservative.
5
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oSIST prEN 12404:2019
prEN 12404:2018 (E)
1 Scope
This document specifies a method for determining the performance of a preservative, applied to the
upper surface of the mortar test specimens, in preventing the growth of dry rot through the treated
mortar when exposed to the test fungus.
This method is only applicable to masonry fungicides applied as a true solution of the preservative in
water or dilute oil in water emulsion. It is not applicable to rods, pastes and other similar preservative
types. This method is applicable to preservatives applied to masonry by brushing, spraying and/or
injection techniques or mixed into rendering and plastering mortar for masonry.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 113:1996, Wood preservatives - Test method for determining the protective effectiveness against wood
destroying basidiomycetes - Determination of the toxic values
EN 413-1, Masonry cement – Part 1: Composition, specifications and conformity criteria
EN 459-1, Building lime - Part 1: Definitions, specifications and conformity criteria
EN 599-1, Durability of wood and wood-based products - Efficacy of preventive wood preservatives as
determined by biological tests - Part 1: Specification according to use class
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at http://www.iso.org/obp
3.1
masonry fungicide
fungicidal/fungistatic product applied to masonry and other mineral construction materials to prevent
the growth of dry rot through or over the treated material
3.2
performance
behaviour of the preservative product in terms of its effectiveness in test
3.3
preservative
formulated masonry fungicide in the form received from the supplier for the test
3.4
supplier
sponsor of the test
6
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oSIST prEN 12404:2019
prEN 12404:2018 (E)
4 Principle
The preservative to be tested is applied by pipette (or in accordance with the sponsor's instruction) to
the upper surface of mortar test specimens. The mortar test specimens are contained in rigid tubes and
an untreated wooden sample is placed on top of these mortar test specimens. The bases of the mortar
specimens are exposed to dry rot attack for a given time. The assessment of the performance of the test
preservative consists of checking the growth of the fungus through the mortar and the evaluation of any
attack of the wooden sample contained in the rigid tube.
5 Test materials
5.1 Test fungus:
5.1.1 Obligatory test fungus:
— Serpula lacrymans (Schumacher ex Fries) S.F. Gray, strain BAM Ebw.315.
5.1.2 Optional test fungi:
For specific regional uses or conditions, it is also possible to use other strains of dry rot (e.g. Serpula
lacrymans FPRL 12 C) known to be capable of growing through masonry.
NOTE Other fungal species can grow through masonry. This method of test could be used to assess the ability
of these fungi to grow through mortar specimens.
5.1.3 Maintenance of strains:
The strains shall be maintained and treated in accordance with the instructions from their laboratory of
origin (see Annex A). If a strain shows signs of degeneration, it shall no longer be used and the testing
laboratory shall obtain a new standard culture of the strain.
5.2 Products and reagents:
5.2.1 Water, distilled or deionized, conform to grade 3 of EN ISO 3696.
5.2.2 Malt – mineral salt – agar culture medium; consisting:
— malt extract
in concentrated form 12,50 g;
or in powder form 10,00 g;
— agar causing no inhibition of growth of fungi
15,00 g;
— potassium dihydrogen phosphate (KH2PO4)
2,72 g;
— calcium sulfate dihydrate (CaSO4.2H2O)
0,38 g;
— magnesium sulfate heptahydrate (MgSO4.7H2O)
0,62 g;
— water (5.2.1) to make up to 1 000 ml.
Place all the ingredients in a 1 000 ml beaker measure and gently heat, stirring occasionally, until
completely dissolved.
Pour 150 ml of the culture medium into each culture vessel (5.3.1).
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Close the vessels with screw cap without a hole a quarter of a turn less than full closure and sterilize the
closed vessels in the autoclave (5.3.8) at (121 ± 2) °C for 30 min. Let them cool standing upright.
5.2.3 Nutrient solution, a mass fraction for 5 % aqueous solution of malt extract.
5.2.4 Equipment for chemical gas or for steam sterilization or access to a radiation source
(see Annex B).
5.2.5 Carbon dioxide, compressed gas in cylinders.
5.2.6 Sodium chloride, saturated solution in water.
5.2.7 Portland cement, conforming to EN 413-1.
5.2.8 Hydrated building lime, conforming to EN 459-1.
5.2.9 Bricklaying mortar sand, quartz sand with a particle size equal to or less than 1 mm, washed
under running tap water until the water is no longer turbid.
5.3 Apparatus:
5.3.1 Culture vessels:
Straight sided flat bottom glass culture vessels with an aperture of 50 mm to 60 mm (see Figure 3),
provided with both screw caps without a hole, used for culturing the test fungus (9.1), and screw caps
with a central hole equal in size to the outer diameter of the tube (5.3.2.) plus the thickness of the tubing
(5.3.3.) in diameter.
NOTE The alternative type C.2 of test vessels described in EN 113 has been found to be suitable.
5.3.2 Rigid tubes, which can be sterilized using an autoclave (for example glass, or polyvinylidene
fluoride) with an inner diameter of 35 mm to 46 mm and a length of at least 150 mm.
5.3.3 Tubing, with a diameter corresponding to the outer diameter of the rigid tubes (5.3.2.) with a
wall thickness of (1,0 ± 0,5) mm and cut into lengths of (40,0 ± 1,0) mm capable of being sterilized using
an autoclave.
NOTE Tubing made of rubber has been found to be suitable.
5.3.4 Inert supports of maximum thickness 3 mm and when in use, do not obscure more than 10 % of
the mortar surface.
Stainless steel washers of overall diameter 25 mm have been found to be suitable. Two supports are
required for each test assembly.
5.3.5 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative
humidity.
5.3.6 Culture chamber, dark and controlled at (22 ± 1) °C and (70 ± 5) % relative humidity.
5.3.7 Drying oven, capable of being controlled at (45 ± 1) °C.
5.3.8 Autoclave, adjustable to (121 ± 2) °C.
5.3.9 Containers, to prepare the mortar and the preservative solutions, made of a material that does
not react with their contents.
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5.3.10 Mortar mould apparatus, consisting of a plastic frame for example polyvinyl chloride (PVC) with
a height of (10 ± 0,5) mm, a porous support (for example clay house building bricks, ceramic plates) to
absorb the excess water in the fresh mortar and a cloth (for example muslin cloth, cheese cloth) with the
same dimensions as the frame to aid demoulding the mortar specimens (see Figure 2).
5.3.11 Plastic lath, used to smooth the surface of the mortar after casting in the plastic frame.
5.3.12 Circular tamper with a flat base, a diameter of 3 mm to 5 mm to less than the internal diameter
of the rigid tube (5.3.2), and at least 50 mm longer than the rigid tube.
5.3.13 Racks on which to place the treated mortar test specimens an example is shown in Figure 1.
Dimensions in millimetres
Key
1 wad of cotton wool
2 tubing
3 culture vessel screw cap
4 rigid tube
5 mortar specimen
Figure 1 — Example of a rack
5.3.14 Sterile single-use pipettes of (1,0 ± 0,1) ml content.
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5.3.15 Ordinary laboratory equipment, including for example balances accurate to 0,01 g, sealable
containers, forceps.
5.3.16 Microbiological safety cabinet, providing protection to the operator and to the work.
6 Sample of the preservative
The sample shall be representative of the product to be tested. It shall be identified as specified in
EN 599-1.
7 Mortar test specimen
7.1 Preparation of mortar
Dry a quantity of the quartz sand (5.2.9) in the drying oven (5.3.7) at (45 ± 1) °C. Measure out nine parts
by volume of the dried quartz sand and weigh, then place into a container (5.3.9). Add two parts by
volume hydrated building lime (5.2.8) and one part by volume Portland cement (5.2.7) and mix
thoroughly. Add 16 ml water (5.2.1) per 100 g quartz s
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