iTeh StandardsiTeh Standards
EURUSD
Your shopping cart is empty.
SIST

SIST EN 17203:2021

(Main)

Foodstuffs - Determination of citrinin in food by HPLC-MS/MS

Foodstuffs - Determination of citrinin in food by HPLC-MS/MS

This document specifies a procedure for the determination of the citrinin content in food (cereals, red yeast rice (RYR)), herbs and food supplements by liquid chromatography tandem mass spectrometry (LC-MS/MS).
This method has been validated for citrinin in red yeast rice and in the formulated food supplements in the range of 2,5 µg/kg to 3000 µg/kg and in wheat flour in the range of 2,5 µg/kg to 100 µg/kg.
Laboratory experiences have shown that this method is also applicable to white rice, herbs such as a powder of ginkgo biloba leaves and the formulated food supplements in the range of 2,5 µg/kg to 50 µg/kg.

Lebensmittel - Bestimmung von Citrinin in Lebensmitteln mit HPLC-MS/MS

Dieses Dokument beschreibt ein Verfahren zur Bestimmung des Citriningehalts in Lebensmitteln (Getreide, rotem Reis [RYR, en: red yeast rice]), Kräutern und Nahrungsergänzungsmitteln mittels Flüssigchromatographie und Tandem-Massenspektrometrie (LC-MS/MS).
Dieses Verfahren wurde für Citrinin in rotem Reis und in entsprechend zusammengesetzten Nahrungsergänzungsmitteln im Bereich von 2,5 μg/kg bis 3 000 μg/kg sowie für Citrinin in Weizenmehl im Bereich von 2,5 μg/kg bis 100 μg/kg validiert.
Laborerfahrungen haben gezeigt, dass dieses Verfahren auch auf weißen Reis, auf Kräuter wie Ginkgo-Biloba-Blätter in Pulverform und entsprechend zusammengesetzte Nahrungsergänzungsmittel im Bereich von 2,5 μg/kg bis 50 μg/kg anwendbar ist.

Produits alimentaires - Dosage de la citrinine dans les produits alimentaires par CLHP-SM/SM

Le présent document décrit un mode opératoire de détermination de la teneur en citrinine dans les aliments [céréales, levure de riz rouge (LRR)], les herbes aromatiques et les compléments alimentaires par chromatographie liquide couplée à une spectrométrie de masse en tandem (CL-SM/SM).
Cette méthode a été validée pour la citrinine dans la levure de riz rouge et dans les compléments alimentaires à base de levure de riz rouge dans la gamme de concentration de 2,5 μg/kg à 3 000 μg/kg et dans la farine de blé dans la gamme de concentration de 2,5 μg/kg à 100 μg/kg.
Les expériences en laboratoire ont montré que cette méthode est également applicable au riz blanc, aux herbes aromatiques telles que les feuilles de Ginkgo biloba en poudre et aux compléments alimentaires formulés dans la gamme de concentration de 2,5 μg/kg à 50 μg/kg.

Živila - Določevanje citrinina v živilih z metodo HPLC-MS/MS

General Information

Status
Published
Public Enquiry End Date
19-Dec-2019
Publication Date
28-Jun-2021
ICS
67.050 - General methods of tests and analysis for food products
Technical Committee
KŽP - Agricultural food products
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
14-Jun-2021
Due Date
19-Aug-2021
Completion Date
29-Jun-2021
Directive
2017/625 - Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official controls and other official activities performed to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant protection products
882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
Ref Project
EN 17203:2021 - Foodstuffs - Determination of citrinin in food by HPLC-MS/MS

Relations

Replaces
SIST EN 17203:2019 - Foodstuffs - Determination of citrinin in food by liquid chromatography tandem mass spectrometry (LC-MS/MS)
Effective Date
08-Jun-2022
Replaces
SIST EN 17203:2019 - Foodstuffs - Determination of citrinin in food by liquid chromatography tandem mass spectrometry (LC-MS/MS)
Effective Date
01-Sep-2021

Buy Standard

EN 17203:2021EN 17203:2021
PreviousNext
Standard
EN 17203:2021
English language
23 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17203:2021
01-september-2021
Nadomešča:
SIST EN 17203:2019
Živila - Določevanje citrinina v živilih z metodo HPLC-MS/MS
Foodstuffs - Determination of citrinin in food by HPLC-MS/MS
Lebensmittel - Bestimmung von Citrinin in Lebensmitteln mit HPLC-MS/MS
Produits alimentaires - Dosage de la citrinine dans les produits alimentaires par CLHP-
SM/SM
Ta slovenski standard je istoveten z: EN 17203:2021
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN 17203:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 17203:2021

---------------------- Page: 2 ----------------------
SIST EN 17203:2021


EN 17203
EUROPEAN STANDARD

NORME EUROPÉENNE

May 2021
EUROPÄISCHE NORM
ICS 67.050 Supersedes EN 17203:2018
English Version

Foodstuffs - Determination of citrinin in food by HPLC-
MS/MS
Produits alimentaires - Dosage de la citrinine dans les Lebensmittel - Bestimmung von Citrinin in
produits alimentaires par CLHP-SM/SM Lebensmitteln mit HPLC-MS/MS
This European Standard was approved by CEN on 19 March 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17203:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN 17203:2021
EN 17203:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus and equipment . 8
7 Procedure. 9
7.1 Preparation of the test sample . 9
7.2 Extraction of citrinin . 9
7.3 Spiking procedure . 10
7.4 Preparation of sample test solutions . 10
7.5 LC-MS/MS analysis .
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.

This May Also Interest You

SIST
SIST EN 17851:2023(Main)
Foodstuffs - Determination of elements and their chemical species - Determination of Ag, As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Se, Tl, U and Zn in foodstuffs by inductively coupled plasma mass spectrometry (ICP-MS) after pressure digestion
EN 17851:2023

This document specifies a procedure for the determination of Ag, As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Se, Tl,
U and Zn in foodstuffs by inductively coupled plasma mass spectrometry (ICP-MS) after pressure
digestion.
The following foodstuffs were analysed for the elements listed in Table 1 in an interlaboratory study:
Banana (deep-frozen), Cocoa powder, Wheat noodle powder, Currant nectar (deep-frozen), Milk powder,
Oyster (dried), Celery (dried), Dogfish liver (dried), Liver (deep-frozen), Kale (dried).

  • Standard
    39 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 15634-3:2023(Main)
Foodstuffs - Detection of food allergens by molecular biological methods - Part 3: Hazelnut (Corylus avellana) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
EN 15634-3:2023

This method describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in
chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut
detected from the gene for corA 1.

  • Standard
    16 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN ISO 20813:2023(Main)
Molecular biomarker analysis - Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) - General requirements and definitions (ISO 20813:2019)
EN ISO 20813:2022

This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods.
The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods.
It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.

  • Standard
    34 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 15634-4:2023(Main)
Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
EN 15634-4:2023

This method describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in
chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4, 5].

  • Standard
    16 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 15634-5:2023(Main)
Foodstuffs - Detection of food allergens by molecular biological methods - Part 5: Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR
EN 15634-5:2023

This method specifies a procedure for the qualitative detection of species specific DNA from
white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex realtime
PCR based on the genes MADS-D (mustard) and lectin (soya). A mustard content of 10
mg/kg or greater and a soya content of 10 mg/kg or greater can be detected with a probability of
> 95 %.

  • Standard
    17 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN ISO 22753:2023(Main)
Molecular biomarker analysis - Method for the statistical evaluation of analytical results obtained in testing sub-sampled groups of genetically modified seeds and grains - General requirements (ISO 22753:2021, Corrected version 2022-11)
EN ISO 22753:2022

This document describes general requirements, procedures and performance criteria for evaluating the content of genetically modified (GM) seeds/grains in a lot by a group testing strategy that includes qualitative analysis of sub-sampled groups followed by statistical evaluation of the results.
This document is applicable to group testing strategy estimating the GM content on a percentage seed/grain basis for purity estimation, testing towards a given reject/accept criterion and for cases where seed/grain lots are carrying stacked events.
This document is not applicable to processed products.
NOTE       Description of the use of group testing strategy are available in References [1], [7], [8], [18], [19] and [20].

  • Standard
    34 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 17641:2022(Main)
Foodstuffs - Multimethod for the determination of aflatoxins, deoxynivalenol, fumonisins, ochratoxin A, T-2 toxin, HT-2 toxin and zearalenone by LC-MS/MS
EN 17641:2022

This document describes an isotope dilution method for the quantitative determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS).
A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and OTA in infant foods (e.g. infant cereals, milk-based powders), in spices, in dried fruits and in nuts.
The method has been validated through an intercollaborative study on different commodity groups: cereals and cereal-based products including food for infant and young children, nuts, spices, dried fruits and milk powder. The ranges of concentrations of each mycotoxin in these naturally contaminated and/or spiked food samples were:
-   aflatoxin B1:   0,0857 µg/kg - 11,4 µg/kg;
-   aflatoxin B2:   0,0792 µg/kg - 12,5 µg/kg;
-   aflatoxin G1:   0,0628 µg/kg - 20,9 µg/kg;
-   aflatoxin G2:   0,0520 µg/kg - 15,0 µg/kg;
-   aflatoxin M1:   0,0342 µg/kg - 0,110 µg/kg;
-   ochratoxin A:   0,448 µg/kg - 17,2 µg/kg;
-   deoxynivalenol:   45,2 µg/kg - 743 µg/kg;
-   zearalenone:   9,57 µg/kg - 131 µg/kg;
-   T-2 toxin:      10,3 µg/kg - 57,9 µg/kg;
-   HT-2 toxin:   9,50 µg/kg - 81,8 µg/kg;
-   fumonisin B1:   31,1 µg/kg - 4 262 µg/kg;
-   fumonisin B2:   44,2 µg/kg - 1 299 µg/kg.
The measuring ranges of the method for each mycotoxin/matrix combination are given in Table 7.

  • Standard
    49 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 17644:2022(Main)
Foodstuffs - Detection of food allergens by liquid chromatography - mass spectrometry (LC-MS) methods - General considerations
EN 17644:2022

This document establishes an overall framework covering qualitative and quantitative methods for the determination of food allergens and allergenic ingredients using mass spectrometry-based methods for the determination of specific peptides/proteins. This document provides general guidelines and performance criteria applicable to this methodology. Guidelines, minimum requirements and performance criteria laid down in this document are intended to ensure that comparable and reproducible results are obtained by different analysts, instrumentation and laboratories.

  • Standard
    20 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 13708:2022(Main)
Foodstuffs - Detection of irradiated foodstuff containing crystalline sugar by ESR spectroscopy
EN 13708:2022

This European Standard specifies a method for the detection of foods containing crystalline sugars which have been treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also called electron paramagnetic resonance (EPR) spectrum, of the food.
Interlaboratory studies have been successfully carried out on dried figs, dried mangoes, dried papayas and raisins.

  • Standard
    12 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 1787:2022(Main)
Foodstuff - Detection of irradiated foodstuff containing cellulose by ESR spectroscopy
EN 1787:2022

This European Standard specifies a method for the detection of foods containing cellulose which have been treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also called electron paramagnetic resonance (EPR) spectrum, of the food.
Interlaboratory studies have been successfully carried out with pistachio nut shells, paprika powder and fresh strawberries. However, it has been shown that false positive results can appear when analysing bleached nuts. For further information, see Clause 7 on limitations.

  • Standard
    16 pages
    English language
    sale 10% off
    e-Library read for
    1 day