Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30 degrees C (ISO 4833:2003)

ISO 4833:2003 specifies a horizontal method for the enumeration of microorganisms, by counting the colonies growing in a solid medium after aerobic incubation at 30 degrees Celsius. Subject to some limitations, it is applicable to products intended for human consumption or the feeding of animals.
The applicability of ISO 4833:2003 to the examination of certain fermented food and animal feeding stuffs is limited. For the examination of fermented food and animal feeding stuffs, other media and/or incubation conditions might be more appropriate.

Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren für die Zählung von Mikroorganismen - Koloniezählverfahren bei 30 °C (ISO 4833:2003)

Diese Internationale Norm legt ein horizontales Verfahren für die Zählung von Mikroorganismen fest, bei dem die in einem festen Kulturmedium bei aerober Bebrütung bei 30 °C wachsenden Keimkolonien gezählt werden. Mit den in der Einleitung diskutierten Einschränkungen gilt diese Internationale Norm für Produkte, die für den menschlichen Verzehr oder die Tierfütterung bestimmt sind.
Die Eignung dieser Internationalen Norm für die Untersuchung bestimmter fermentierter Lebens  und Futtermittel ist begrenzt. Für die Untersuchung bestimmter fermentierter Lebens  und Futtermittel können andere Kulturmedien und/oder Bebrütungsbedingungen besser geeignet sein.

Microbiologie des aliments - Méthode horizontale pour le dénombrement des micro-organismes - Technique de comptage des colonies a 30 degrés C (ISO 4833:2003)

L'ISO 4833:2003 spécifie une méthode horizontale pour le dénombrement des micro-organismes par comptage des colonies obtenues en milieu solide après incubation en aérobiose à 30 degrés Celsius. En dehors de certaines limitations, elle est applicable aux produits destinés à la consommation humaine ou à l'alimentation animale.
L'application de l'ISO 4833:2003 à l'analyse de certains aliments fermentés est limitée. D'autres milieux et/ou conditions d'incubation peuvent être plus appropriés à l'analyse des aliments fermentés.

Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje števila mikroorganizmov - Tehnika štetja kolonij pri 30 oC (ISO 4833:2003)

General Information

Status
Withdrawn
Publication Date
31-Mar-2003
Withdrawal Date
17-Nov-2013
Current Stage
9900 - Withdrawal (Adopted Project)
Start Date
15-Nov-2013
Due Date
08-Dec-2013
Completion Date
18-Nov-2013

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SLOVENSKI STANDARD
SIST EN ISO 4833:2003
01-april-2003
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje števila
mikroorganizmov - Tehnika štetja kolonij pri 30 oC (ISO 4833:2003)

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of

microorganisms - Colony-count technique at 30 degrees C (ISO 4833:2003)

Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren für die

Zählung von Mikroorganismen - Koloniezählverfahren bei 30 °C (ISO 4833:2003)
Microbiologie des aliments - Méthode horizontale pour le dénombrement des micro-
organismes - Technique de comptage des colonies a 30 degrés C (ISO 4833:2003)
Ta slovenski standard je istoveten z: EN ISO 4833:2003
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 4833:2003 en

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
EUROPEAN STANDARD
EN ISO 4833
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2003
ICS 07.100.30
English version
Microbiology of food and animal feeding stuffs - Horizontal
method for the enumeration of microorganisms - Colony-count
technique at 30 degrees C (ISO 4833:2003)

Microbiologie des aliments - Méthode horizontale pour le Mikrobiologie von Lebensmitteln und Futtermitteln -

dénombrement des micro-organismes - Technique de Horizontales Verfahren für die Zählung von

comptage des colonies à 30 degrés C (ISO 4833:2003) Mikroorganismen - Koloniezählverfahren bei 30 °C (ISO

4833:2003)
This European Standard was approved by CEN on 2 January 2003.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official

versions.

CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,

Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and United

Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels

© 2003 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 4833:2003 E

worldwide for CEN national Members.
---------------------- Page: 2 ----------------------
EN ISO 4833:2003 (E)
CORRECTED 2003-03-26
Foreword

This document (EN ISO 4833:2003) has been prepared by Technical Committee ISO/TC 34

"Agricultural food products" in collaboration with Technical Committee CEN/TC 302 "Milk and

milk products - Methods of sampling and analysis", the secretariat of which is held by NEN.

This European Standard shall be given the status of a national standard, either by publication of

an identical text or by endorsement, at the latest by August 2003, and conflicting national

standards shall be withdrawn at the latest by August 2003.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of

the following countries are bound to implement this European Standard: Austria, Belgium, Czech

Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,

Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and

the United Kingdom.
Endorsement notice

The text of ISO 4833:2003 has been approved by CEN as EN ISO 4833:2003 without any

modifications.

NOTE Normative references to International Standards are listed in Annex ZA (normative).

---------------------- Page: 3 ----------------------
EN ISO 4833:2003 (E)
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications

This European Standard incorporates by dated or undated reference, provisions from other

publications. These normative references are cited at the appropriate places in the text and the

publications are listed hereafter. For dated references, subsequent amendments to or revisions of

any of these publications apply to this European Standard only when incorporated in it by

amendment or revision. For undated references the latest edition of the publication referred to

applies (including amendments).

NOTE Where an International Publication has been modified by common modifications, indicated

by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 6887-1 1999 Microbiology of food and animal EN ISO 6887-1 1999
feeding stuffs - Preparation of test
samples, initial suspension and
decimal dilutions for
microbiological examination - Part
1: General rules for the preparation
of the initial suspension and
decimal dilutions
ISO 8261 2001 Milk and milk products - General EN ISO 8261 2001
guidance for the preparation of test
samples, initial suspensions and
decimal dilutions for
microbiological examination
ISO/TR 11133-1 2000 Microbiology of food and animal ENV ISO 11133-1 2000
feeding stuffs - Guidelines on
preparation and production of
culture media - Part 1: General
guidelines on quality assurance for
the preparation of culture media in
the laboratory
---------------------- Page: 4 ----------------------
INTERNATIONAL ISO
STANDARD 4833
Third edition
2003-02-01
Microbiology of food and animal feeding
stuffs — Horizontal method for the
enumeration of microorganisms —
Colony-count technique at 30 °C
Microbiologie des aliments — Méthode horizontale pour le
dénombrement des micro-organismes — Technique de comptage des
colonies à 30 °C
Reference number
ISO 4833:2003(E)
ISO 2003
---------------------- Page: 5 ----------------------
ISO 4833:2003(E)
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ii © ISO 2003 — All rights reserved
---------------------- Page: 6 ----------------------
ISO 4833:2003(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies

(ISO member bodies). The work of preparing International Standards is normally carried out through ISO

technical committees. Each member body interested in a subject for which a technical committee has been

established has the right to be represented on that committee. International organizations, governmental and

non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the

International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.

The main task of technical committees is to prepare International Standards. Draft International Standards

adopted by the technical committees are circulated to the member bodies for voting. Publication as an

International Standard requires approval by at least 75 % of the member bodies casting a vote.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO 4833 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,

Microbiology.

This third edition cancels and replaces the second edition (ISO 4833:1991). The following technical changes

have been made:
 subclause 5.2, Plate count agar: an examination of dairy products is included;

 Clause 10, Expression of results: precision data are given, and an example of precision data for dairy

products.
© ISO 2003 — All rights reserved iii
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ISO 4833:2003(E)
Introduction

Because of the large variety of food and feed products, this horizontal method may not be appropriate in every

detail for certain products. In this case, different methods which are specific to these products may be used if

absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this

horizontal method as far as possible.

When this International Standard is next reviewed, account will be taken of all information then available

regarding the extent to which this horizontal method has been followed and the reasons for deviations from

them in the case of particular products.

The harmonization of test methods cannot be immediate, and for certain groups of products International

Standards and/or national standards may already exist that do not comply with this horizontal method. In

cases where International Standards already exist for the product to be tested, they should be followed. It is

hoped that when such standards are reviewed, they will be changed to comply with this International Standard

so that eventually the only remaining departures from this horizontal method will be those necessary for well-

established technical reasons.
iv © ISO 2003 — All rights reserved
---------------------- Page: 8 ----------------------
INTERNATIONAL STANDARD ISO 4833:2003(E)
Microbiology of food and animal feeding stuffs — Horizontal
method for the enumeration of microorganisms —
Colony-count technique at 30 °C
1 Scope

This International Standard specifies a horizontal method for the enumeration of microorganisms, by counting

the colonies growing in a solid medium after aerobic incubation at 30 °C. Subject to the limitations discussed

in the introduction, this International Standard is applicable to products intended for human consumption or

the feeding of animals.

The applicability of this International Standard to the examination of certain fermented food and animal

feeding stuffs is limited. For the examination of fermented food and animal feeding stuffs, other media and/or

incubation conditions might be more appropriate.
2 Normative references

The following referenced documents are indispensable for the application of this document. For dated

references, only the edition cited applies. For undated references, the latest edition of the referenced

document (including any amendments) applies.

ISO 6887 (all parts), Microbiology of food and animal feeding stuffs — Preparation of test samples, initial

suspension and decimal dilutions for microbiological examination

ISO 7218:1996, Microbiology of food and animal feeding stuffs — General rules for microbiological

examinations

ISO 8261, Milk and milk products — General guidance for the preparation of test samples, initial suspensions

and decimal dilutions for microbiological examination

ISO/TS 11133-1, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production

of culture media — Part 1: General guidelines on quality assurance for the preparation of culture media in the

laboratory
3 Term and definition
For the purposes of this document, the following term and definition applies.
3.1
microorganism

bacteria, yeast and mould-forming countable colony, produced under the conditions specified in this

International Standard
© ISO 2003 — All rights reserved 1
---------------------- Page: 9 ----------------------
ISO 4833:2003(E)
4 Principle

4.1 Two poured plates are prepared using a specified culture medium and a specified quantity of the test

sample, if the initial product is liquid, or using a specified quantity of an initial suspension in the case of other

products.

Other pairs of poured plates are prepared, under the same conditions, using decimal dilutions of the test

sample or of the initial suspension.
4.2 The plates are aerobically incubated at 30 °C for 72 h.

4.3 The number of microorganisms per millilitre or per gram of sample is calculated from the number of

colonies obtained on selected plates (see Clause 10).
5 Culture media and diluents
For current laboratory practice, see ISO 7218 and ISO/TS 11133-1.
5.1 Diluents
See the relevant part of ISO 6887.
5.2 Plate count agar (PCA)
5.2.1 Composition
Enzymatic digestion of casein 5,0 g
Yeast extract 2,5 g
Glucose, anhydrous (C H O ) 1,0 g
6 12 6
9 g to 18 g
Agar
Water 1 000 ml

When dairy products are examined, add 1,0 g of skimmed milk powder per litre of the culture medium. The

skimmed milk powder shall be free from inhibitory substances.
5.2.2 Preparation
5.2.2.1 Preparation from commercial dehydrated complete medium
...

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