SIST EN 14132:2003

Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up

This European Standard specifies a method for the determination of ochratoxin A content in barley up to 6 ug/kg and ochratoxin A content in roasted coffee up to 6 ug/kg using immunoaffinity (IA) column clean up and high performance liquid chromatography (HPLC).

Lebensmittel - Bestimmung von Ochratoxin A in Gerste und Röstkaffee - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule

Produits alimentaires - Dosage de l'ochratoxine A dans l'orge et dans le café torréfié - Méthode par purification sur colonne d'immunoaffinité suivie d'une analyse par CLHP

La présente Norme européenne spécifie une méthode de dosage de l'ochratoxine A dans l'orge et dans le café torréfié par purification sur colonne d'immunoaffinité et par chromatographie liquide haute performance (CLHP). Cette méthode a été validée pour une teneur en ochratoxine A de 0,1 mg/kg à 4,5 mg/kg dans l'orge et de 0,2 mg/kg à 5,5 mg/kg dans le café torréfié.

Živila - Določevanje ohratoksina A v ječmenu in praženi kavi - Metoda HPLC z imunoafinitetnim kolonskim čiščenjem

General Information

• Status: Withdrawn
• Publication Date: 31-Oct-2003
• Withdrawal Date: 15-Jun-2009
• ICS: 67.140.20 - Coffee and coffee substitutes
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 16-Jun-2009
• Due Date: 09-Jul-2009
• Completion Date: 16-Jun-2009
• Ref Project: EN 14132:2003 - Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up

Relations

• Replaced By: SIST EN 14132:2009 - Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up
• Effective Date: 01-Sep-2009
• Corrected By: SIST EN 14132:2003/AC:2007 - Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up
• Effective Date: 01-Mar-2007

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Ochratoxin A in Gerste und Röstkaffee - HPLC-Verfahren mit Reinigung an einer ImmunoaffinitätssäuleProduits alimentaires - Dosage de l'ochratoxine A dans l'orge et dans le café torréfié - Méthode par purification sur colonne d'immunoaffinité suivie d'une analyse par CLHPFoodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up67.140.20Kava in kavni nadomestkiCoffee and coffee substitutesICS:Ta slovenski standard je istoveten z:EN 14132:2003SIST EN 14132:2003en01-november-2003SIST EN 14132:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14132May 2003ICS 67.140.20English versionFoodstuffs - Determination of ochratoxin A in barley and roastedcoffee - HPLC method with immunoaffinity column clean-upProduits alimentaires - Dosage de l'ochratoxine A présentedans l'orge et dans le café torréfié - Méthode par CLHP etpar purification en colonne d'immunoaffinitéLebensmittel - Bestimmung von Ochratoxin A in Gerste undRöstkaffee - HPLC-Verfahren mit Reinigung an einerImmunoaffinitätssäuleThis European Standard was approved by CEN on 3 March 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovak Republic, Spain, Sweden, Switzerland andUnited Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14132:2003 ESIST EN 14132:2003

Precision data.13Bibliography.15SIST EN 14132:2003

µm to 25
µm or similar5.12 Disposable syringe filters, of 0,2 m pore size and 25 mm diameter polysulfone membrane5.13 HPLC apparatus, consisting of:5.13.1 Variable injection system, a valve injection system with e.g. a 100 l injection loop5.13.2 HPLC pump, isocratic, pulse free, capable of maintaining a volume flow rate of 1 ml/min5.13.3 Analytical reverse phase separating column, for example C18 octyldecyl
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