EN 14132:2009

Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up

This European Standard specifies a method for the determination of ochratoxin A content in barley and roasted coffee using immunoaffinity column clean up and high performance liquid chromatography (HPLC). This method has been validated for ochratoxin A contents in barley in the range from 0,1 g/kg up to 4,5 g/kg and for roasted coffee in the range from 0,2 g/kg up to 5,5 g/kg.

Lebensmittel - Bestimmung von Ochratoxin A in Gerste und Röstkaffee - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Ochratoxin A in Gerste und Röstkaffee mit Reinigung an einer Immunoaffinitätssäule und Hochleistungs Flüssigchromatographie (HPLC) fest. Das Verfahren wurde für Ochratoxin A Gehalte in Gerste von 0,1 µg/kg bis 4,5 µg/kg und in Röstkaffee von 0,2 µg/kg bis 5,5 µg/kg validiert.

Produits alimentaires - Dosage de l'ochratoxine A dans l'orge et le café torréfié - Méthode par purification sur colonne d'immuno-affinité suivie d'une analyse par chromatographie liquide haute performance (CLHP)

La présente Norme européenne spécifie une méthode de dosage de l’ochratoxine A dans l’orge et dans le
café torréfié par chromatographie liquide à haute performance (CLHP) avec purification sur colonne
d’immuno-affinité. Cette méthode a été validée pour une teneur en ochratoxine A de 0,1 µg/kg à 4,5 µg/kg
dans l’orge et de 0,2 µg/kg à 5,5 µg/kg dans le café torréfié.

Živila - Določevanje ohratoksina A v ječmenu in praženi kavi - Metoda HPLC z imunoafinitetnim kolonskim čiščenjem

General Information

• Status: Published
• Publication Date: 26-May-2009
• ICS: 67.140.20 - Coffee and coffee substitutes
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 5 - Biotoxins
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 28-Oct-2020
• Ref Project: SIST EN 14132:2009 - Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up

Relations

• Replaces: EN 14132:2003 - Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up
• Effective Date: 30-May-2009
• Replaces: EN 14132:2003/AC:2006 - Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up
• Effective Date: 30-May-2009

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Ochratoxin A in Gerste und Röstkaffee - HPLC-Verfahren mit Reinigung an einer ImmunoaffinitätssäuleProduits alimentaires - Dosage de l'ochratoxine A dans l'orge et le café torréfié - Méthode par purification sur colonne d'immuno-affinité suivie d'une analyse par chromatographie liquide haute performance (CLHP)Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean-up67.140.20Kava in kavni nadomestkiCoffee and coffee substitutesICS:Ta slovenski standard je istoveten z:EN 14132:2009SIST EN 14132:2009en,fr,de01-september-2009SIST EN 14132:2009SLOVENSKI
STANDARDSIST EN 14132:2003/AC:2007SIST EN 14132:20031DGRPHãþD



SIST EN 14132:2009



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14132May 2009ICS 67.140.20Supersedes EN 14132:2003
English VersionFoodstuffs - Determination of ochratoxin A in barley and roastedcoffee - HPLC method with immunoaffinity column clean-upProduits alimentaires - Dosage de l'ochratoxine A dansl'orge et le café torréfié - Méthode par purification surcolonne d'immuno-affinité suivie d'une analyse parchromatographie liquide haute performance (CLHP)Lebensmittel - Bestimmung von Ochratoxin A in Gerste undRöstkaffee - HPLC-Verfahren mit Reinigung an einerImmunoaffinitätssäuleThis European Standard was approved by CEN on 24 March 2009.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre:
Avenue Marnix 17,
B-1000 Brussels© 2009 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14132:2009: ESIST EN 14132:2009



EN 14132:2009 (E) 2 Contents Foreword . 3 1 Scope . 4 2 Normative reference . 4 3 Principle . 4 4 Reagents . 4 5 Apparatus . 6 6 Procedure . 7 7 Spiking procedure . 8 8 HPLC analysis . 9 9 Calculation . 10 10 Precision . 10 11 Test report . 11 Annex A (informative)
Precision data . 12 Bibliography .
...