This document specifies the atmosphere for the conditioning and testing of samples of tobacco and tobacco products.
It is primarily applicable to cigarettes; however, if not specified differently in other ISO standards, it can also be applied to tobacco, other tobacco products, and materials used in the manufacture of tobacco products for which prior conditioning is necessary. Standards for the conditioning and testing of other forms of tobacco, tobacco products, and materials can refer to all or part of this document.

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This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISOÂ 4387.
NOTEÂ Â Â Â Â Â ISOÂ 20778 and ISOÂ 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

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This document specifies methods for the determination of total particulate matter and for the subsequent determination of nicotine-free dry particulate matter present in the smoke from cigarettes generated and collected using a routine analytical smoking machine.

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ISO/IEC 17025:2017 specifies the general requirements for the competence, impartiality and consistent operation of laboratories.
ISO/IEC 17025:2017 is applicable to all organizations performing laboratory activities, regardless of the number of personnel.
Laboratory customers, regulatory authorities, organizations and schemes using peer-assessment, accreditation bodies, and others use ISO/IEC 17025:2017 in confirming or recognizing the competence of laboratories.

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This International Standard: - defines smoking parameters and specifies the standard conditions to be provided for the routine analytical machine smoking of cigarettes; - specifies the requirements for a routine analytical smoking machine complying with the standard conditions. Annex A specifies the ambient air velocities surrounding cigarettes in a routine analytical smoking machine, the mechanical design of the enclosures immediately surrounding them, and the methods of air velocity measurement including the location where air velocity is measured. Annex B describes, as an example, the special characteristics of a typical smoking machine incorporating a piston type of puffing mechanism. Annex C includes a diagram of a puff profile and illustrates definitions and standard conditions.

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This International Standard specifies two methods of providing representative samples of a population of cigarettes manufactured for sale. Different procedures are specified (see Table 1) according to whether sampling is undertaken at the point of sale or at a factory.
a) Sampling “at one point in time” provides for appraisal of the chosen properties of the cigarettes on that occasion. Sampling is carried out within as short a period as possible.
b) Sampling “over a period of time” provides for on-going appraisals. It can be considered for practical purposes as a series of samples each taken “at one point in time”.

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This International Standard specifies a method for the determination of carbon monoxide (CO) in the vapour phase of cigarette smoke.

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