Standard Test Method for Laboratory Oil Spill Dispersant Effectiveness Using the Baffled Flask

SIGNIFICANCE AND USE
5.1 A standard test is necessary to establish a baseline performance parameter so that dispersants can be compared, a given dispersant can be compared for effectiveness on different oils, and at different oil weathering stages, and batches of dispersant or oils can be checked for effectiveness changes with time or other factors. This test method provides a second test at higher mixing energy in addition to the Swirling Flask (Test Method F2059).  
5.2 Dispersant effectiveness varies with oil type, sea energy, oil conditions, salinity, and many other factors. Test results from this test method form a baseline at high mixing energy, but are not to be taken as the absolute measure of performance at sea. Actual field effectiveness could be more or less than this value.  
5.3 Many dispersant tests have been developed around the world. This test has been developed in recent years and provides higher mixing energies compared to other laboratory scale tests.
SCOPE
1.1 This test method covers the procedure to determine the effectiveness of oil spill dispersants on various oils in the laboratory. This test method is not applicable to other chemical agents nor to the use of such products or dispersants in open waters.  
1.2 This test method covers the use of the Baffled Flask test apparatus and does not cover other apparatuses nor are the analytical procedures described in this report directly applicable to such procedures.  
1.3 The test results obtained using this test method are intended to provide baseline effectiveness values used to compare dispersants and oil types under conditions analogous to those used in the test.  
1.4 The test results obtained using this test method are effectiveness values that should be cited as test values derived from this standard test. Dispersant effectiveness values do not directly relate to effectiveness at sea or in other apparatuses. Actual effectiveness at sea is dependent on sea energy, oil state, temperature, salinity, actual dispersant dosage, and amount of dispersant that interacts with the oil.  
1.5 The decision to use or not use a dispersant on an oil should not be based solely on this or any other laboratory test method.  
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

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Publication Date
31-Aug-2021
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: F3251 − 21
Standard Test Method for
Laboratory Oil Spill Dispersant Effectiveness Using the
1
Baffled Flask
This standard is issued under the fixed designation F3251; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom-
1.1 This test method covers the procedure to determine the
mendations issued by the World Trade Organization Technical
effectiveness of oil spill dispersants on various oils in the
Barriers to Trade (TBT) Committee.
laboratory.This test method is not applicable to other chemical
agents nor to the use of such products or dispersants in open
2. Referenced Documents
waters.
2
2.1 ASTM Standards:
1.2 This test method covers the use of the Baffled Flask test
F2059 Test Method for Laboratory Oil Spill Dispersant
apparatus and does not cover other apparatuses nor are the
Effectiveness Using the Swirling Flask
analytical procedures described in this report directly appli-
3
2.2 EPA Standard:
cable to such procedures.
SW-846 Method 8270D, Revision 4 Semivolatile Organic
1.3 The test results obtained using this test method are
Compounds by Gas Chromatography/Mass Spectrometry
intended to provide baseline effectiveness values used to
(GC/MS)
compare dispersants and oil types under conditions analogous
to those used in the test.
3. Terminology
1.4 The test results obtained using this test method are
3.1 Definitions:
effectiveness values that should be cited as test values derived
3.1.1 effectiveness, n—the capability of producing a desired
from this standard test. Dispersant effectiveness values do not
result – which is this case is the dispersion of oil into water.
directly relate to effectiveness at sea or in other apparatuses.
3.1.1.1 Discussion—Effectiveness is given here as the per-
Actualeffectivenessatseaisdependentonseaenergy,oilstate,
cent of oil dispersed into the water column as a result of
temperature, salinity, actual dispersant dosage, and amount of
dispersant action and energy.
dispersant that interacts with the oil.
3.1.2 trypsinizing, n—the process of cell dissociation using
1.5 The decision to use or not use a dispersant on an oil
trypsin, an enzyme which breaks down proteins, to dissociate
should not be based solely on this or any other laboratory test
adherent cells from the vessel in which they are being cultured.
method.
3.1.2.1 Discussion—This test method uses only the vessel
1.6 The values stated in SI units are to be regarded as
designed for that process (with the addition of a withdrawal
standard. No other units of measurement are included in this
tube).
standard.
4. Summary of Test Method
1.7 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
4.1 Thedispersantispre-mixedwithoilandplacedonwater
responsibility of the user of this standard to establish appro-
in a test vessel. The test vessel is agitated on a moving table
priate safety, health, and environmental practices and deter-
shaker. At the end of the shaking period, a settling period is
mine the applicability of regulatory limitations prior to use.
specified and then a sample of water taken.The oil in the water
1.8 This international standard was developed in accor-
dance with internationally recognized principles on standard-
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
1
This test method is under the jurisdiction of ASTM Committee F20 on Standards volume information, refer to the standard’s Document Summary page on
Hazardous Substances and Oil Spill Response and is the direct responsibility of the ASTM website.
3
Subcommittee F20.13 on Treatment. AvailablefromUnitedStatesEnvironmentalProtectionAgency(EPA),William
Current edition approved Sept. 1, 2021. Published September 2021. Originally Jefferson Clinton Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460,
approved in 2017. Last previous edition approved in 2017 as F3251–17. DOI: http://www.epa.gov; https://www.epa.gov/sites/production/files/2015-12/
10.1520/F3251-21 documents/8270d.pdf
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
F3251 − 21
column is extracted from the water using
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: F3251 − 17 F3251 − 21
Standard Test Method for
Laboratory Oil Spill Dispersant Effectiveness Using the
1
Baffled Flask
This standard is issued under the fixed designation F3251; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method covers the procedure to determine the effectiveness of oil spill dispersants on various oils in the laboratory.
This test method is not applicable to other chemical agents nor to the use of such products or dispersants in open waters.
1.2 This test method covers the use of the Baffled Flask test apparatus and does not cover other apparatuses nor are the analytical
procedures described in this report directly applicable to such procedures.
1.3 The test results obtained using this test method are intended to provide baseline effectiveness values used to compare
dispersants and oil types under conditions analogous to those used in the test.
1.4 The test results obtained using this test method are effectiveness values that should be cited as test values derived from this
standard test. Dispersant effectiveness values do not directly relate to effectiveness at sea or in other apparatuses. Actual
effectiveness at sea is dependent on sea energy, oil state, temperature, salinity, actual dispersant dosage, and amount of dispersant
that interacts with the oil.
1.5 The decision to use or not use a dispersant on an oil should not be based solely on this or any other laboratory test method.
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and determine the
applicability of regulatory limitations prior to use.
1.8 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
F2059 Test Method for Laboratory Oil Spill Dispersant Effectiveness Using the Swirling Flask
1
This test method is under the jurisdiction of ASTM Committee F20 on Hazardous Substances and Oil Spill Response and is the direct responsibility of Subcommittee
F20.13 on Treatment.
Current edition approved April 15, 2017Sept. 1, 2021. Published May 2017September 2021. Originally approved in 2017. Last previous edition approved in 2017 as
F3251–17. DOI: 10.1520/F3251-1710.1520/F3251-21
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
F3251 − 21
3
2.2 EPA Standard:
SW-846 Method 8270D, Revision 4 Semivolatile Organic Compounds by Gas Chromatography/Mass Spectrometry (GC/MS)
3. Terminology
3.1 Definitions:
3.1.1 effectiveness, n—the capability of producing a desired result – which is this case is the dispersion of oil into water.
3.1.1.1 Discussion—
Effectiveness is given here as the percent of oil dispersed into the water column as a result of dispersant action and energy.
3.1.2 trypsinizing, n—the process of cell dissociation using trypsin, an enzyme which breaks down proteins, to dissociate adherent
cells from the vessel in which they are being cultured.
3.1.2.1 Discussion—
This test method uses only the vessel designed for that process (with the addition of a withdrawal tube).
4. Summary of Test Method
4.1 The dispersant is pre-mixed with oil and placed on water in a test vessel. The test vessel is agitated on a moving table shaker.
At the end of the shaking period, a settling period is specified and then a sample of water taken. The oil in the water column is
extracted from the water using a dichloromethane solvent and analyzed usin
...

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