ASTM F1094-87(1999)

Standard Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method

SCOPE
1.1 These test methods cover sampling and analysis of high purity water from water purification systems and water transmission systems by the direct sampling tap and filtration of the sample collected in the bag. These test methods cover both the sampling of water lines and the subsequent microbiological analysis of the sample by the culture technique. The microorganisms recovered from the water samples and counted on the filters include both aerobes and facultative anaerobes.
1.2 Three methods are described as follows: SectionsTest Method A--Sample Tap-Direct Filtration6 to 8Test Method B--Presterilized Plastic Bag Technique9 to 12Test Method B2--Dip Strip Technique2/Presterilized Plastic Bag
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use .

General Information

• Status: Historical
• Publication Date: 09-Jun-1999
• ICS: 07.100.20 - Microbiology of water
• Technical Committee: F01 - Electronics
• Drafting Committee: F01.10 - Contamination Control

Relations

• Replaced By: ASTM F1094-87(2005) - Standard Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method
• Effective Date: 10-Jun-1999
• Referred By: ASTM D1193-06(2018) - Standard Specification for Reagent Water
• Effective Date: 10-Jun-1999
• Referred By: ASTM E3152-23 - Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
• Effective Date: 10-Jun-1999
• Referred By: ASTM D6974-20 - Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
• Effective Date: 10-Jun-1999
• Referred By: ASTM D5196-06(2018) - Standard Guide for Bio-Applications Grade Water
• Effective Date: 10-Jun-1999
• Referred By: ASTM D5127-13(2018) - Standard Guide for Ultra-Pure Water Used in the Electronics and Semiconductor Industries
• Effective Date: 10-Jun-1999

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:F 1094–87(Reapproved1999)
Standard Test Methods for
Microbiological Monitoring of Water Used for Processing
Electron and Microelectronic Devices by Direct Pressure
Tap Sampling Valve and by the Presterilized Plastic Bag
Method
This standard is issued under the fixed designation F 1094; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 3. Terminology
1.1 These test methods cover sampling and analysis of high 3.1 Definitions:
purity water from water purification systems and water trans- 3.1.1 total bacteria count—number of viable heterotrophic
mission systems by the direct sampling tap and filtration of the bacteria capable of growing under test conditions specified.
sample collected in the bag. These test methods cover both the 3.1.1.1 Discussion—Total bacteria count is the general term
sampling of water lines and the subsequent microbiological for heterotrophic plate count, now commonly used. Het-
analysis of the sample by the culture technique. The microor- erotrophic bacteria are those microorganisms that cannot use
ganisms recovered from the water samples and counted on the CO for food. They require more complex organic compounds
filters include both aerobes and facultative anaerobes. for use as growth nutrients. The majority of bacteria fall into
1.2 Three methods are described as follows: this major grouping.
3.1.2 For definition of other terms used in this test method,
Sections
Test Method A—Sample Tap—Direct Filtration 6 to 8
refer to Terminology D 1129.
Test Method B—Presterilized Plastic Bag Technique 9 to 12
3.2 Definitions of Terms Specific to This Standard:
Test Method B2 —Dip Strip Technique /Presterilized Plastic
3.2.1 presterilized plastic bag—a commercial presterilized
Bag
plastic bag of 200-mL capacity (or as appropriate to larger
1.3 This standard does not purport to address all of the
sample sizes) to hold sample water. The bag should have
safety concerns, if any, associated with its use. It is the
integral fold over tabs to allow for resealing.
responsibility of the user of this standard to establish appro-
3.2.2 bacteriological monitor—a commercial presterilized
priate safety and health practices and determine the applica-
plastic filter holder containing a 0.45-µm membrane filter. (No
bility of regulatory limitations prior to use .
other filter pore size should be used.)
2. Referenced Documents
NOTE 1—If a larger pore size filter is used, organisms may pass through
the filter; a smaller pore size filter does not wick up sufficient growth
2.1 ASTM Standards:
media, hence the level of recovery will be less than that of the 0.45-µm
D 1129 Terminology Relating to Water
filter.
D 1193 Specification for Reagent Water
3.2.3 total count tester—a paddle shaped plastic filter as-
D 3370 Practices for Sampling Water
F 60 Test Methods for Detection and Estimation of Micro- sembly containing a 0.45-µm membrane filter and dehydrated
nutrient pad.
biological Contaminants in Water Used for Processing
Electron and Microelectronic Devices
4. Summary of Test Method
F 488 Test Method for On-Site Screening Heterotrophic
4.1 TestMethodA—SampleTap—DirectFiltration—Asam-
Bacteria in Water
pling valve as or similar to that shown in Fig. 1 is installed in
a pressurized line. The valve illustrated has a self closure and
These test methods are under the jurisdiction of ASTM Committee F-1 on
a male luer outlet fitting. This valve design minimized the
Electronics and are the direct responsibility of Subcommittee F01.10 on Processing
chance of extraneous contamination. Any valve used for
Environments.
sampling should be constructed in a manner to reduce or
Current edition approved Oct. 30, 1987. Published December 1987.
The dip strip (Total Count Tester or SPC Sampler) method is permissible for
prevent the retention of bacteria within its internal surfaces,
waters containing >10 microorganisms per millilitre.
and should be easily sanitized. The bacterial monitor is
Annual Book of ASTM Standards, Vol 11.01.
4 connected to either the luer outlet of the illustrated sampling
Discontinued; see 1991 Annual Book of ASTM Standards, Vol 10.05.
valve,orinasuitablemannertoanequivalentvalve.Thewater
Annual Book of ASTM Standards, Vol 11.02.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
F 1094
FIG. 1 Sampling Valve in Wall of Pressurized Line
sample is passed directly through the monitor, and the effluent The temperature of incubation should always be at 28 6 2°C,
volume is measured after this filtration. Test Methods F 60 are and the period of incubation should be 48 h (or 72 h if time
then employed for bacteriological examination of the sample. permits). The period of incubation and temperature should be
4.2 Test Method B—Presterilized Plastic Bag—The sam- the same for all comparative studies.
pling valve is installed as in Test MethodA, then flushed clean
TEST METHOD A—DIRECT SAMPLE TAP
priortotakingthesamples.Thewatersampleisdirectlyflowed
into a presterilized, precalibrated plastic disposable bag. After
6. Apparatus
sampling, the plastic bag is sealed and stored briefly prior to
6.1 Sampling Tap, see Fig. 1.
bacteriological analysis of the sample. The sample may be
6.2 Bacteriological Monitor with 0.45-µm membrane fil-
stored at room temperature if analyzed within 2 h, otherwise, it
ter.
should be stored from 4 to to 10°C and analyzed within 6 h.
6.3 Sanitarians Kit, consisting of metal syringe, special
4.2.1 Sample analysis is conducted by either Test Methods
two way valve, and stainless steel graduated cup.
F 60 or Test Method F 488 for bacterial content of the water.
6.4 Forceps with blunt stainless, unserrated tips.
6.5 Incubator ,capableofholdingtemperaturewithin 61°C
5. Significance and Use
in a range from 27 to 40°C.
5.1 These test methods provide a field technique for the
6.6 Illuminator, 15 to 30-W incandescent or 8 to 10-W
bacteriological analysis of electronic process waters. The
fluorescent are generally acceptable. If incandescent light is
sampling of these waters and subsequent bacteriological analy-
concentrated through or by a magnifying lens, a lower wattage
sis may be critical to electronic product yields. Bacteria can be
may suffice.
the prime source of harmful contamination which can signifi-
6.7 Magnifier,5to15 3 for counting colonies. An illumi-
cantly reduce the yield of satisfactory microelectronic device
nator hand magnifier or a stereoscopic (dissection-type) micro-
production.
scope are satisfactory.
5.2 The test methods described here may be used both to
6.8 Hypodermic Needle, No. 18, 2-in. blunt nose with
monitor the bacteriological quality of water used in microelec-
plastic syringe.
tronic product processing, and to locate the source of bacterial
contamination in a water purification system.
7. Reagents and Materials
5.3 These test methods are simple field methods, combining
7.1 Isopropyl alcohol,70to90 %,or3to6 %semi-standard
sampling and bacteriological analysis techniques that do not
or reagent grade, hydrogen peroxide solution.
require bacteriological laboratory facilities.
5.4 The test methods described employ culture techniques
The sole source of supply of valves, YY2004000, and YY20E4010 (catalogue
forbacteriologicalanalysis.Theusershouldbeawarethatsuch
number), known to the committee at this time is Millipore Corp., Bedford, MA. If
techniques cannot provide a complete count of the total viable
you are aware of alternative suppliers, please provide this information to ASTM
bacteria present, since clumps and clusters of bacteria will
Headquarters.Your comments will receive careful consideration at a meeting of the
appear as one single colony when cultured, and since some
responsible technical committee, which you may attend.
Thesolesourceofsupplyoftheseproductsknowntothecommitteeatthistime
viable bacteria will not grow under the test conditions used.
is Millipore Corp., Bedford, MA. If you are aware of alternative suppliers, please
However, a meaningful comparative bacteria count will be
provide this information to ASTM Headquarters. Your comments will receive
achievedbythismethodiftheculturingofthesampleisalways
careful consideration at a meeting of the responsible technical committee, which
done at the same temperature, and for the same period of time. you may attend.
F 1094
7.2 Nutrient media —Supplied in double-tip scored am- 8.1.8 Removethemonitorfromtheoutletluerandattachthe
poule or impervious plastic ampoule of either type listed: syringe pump to the outlet side of the monitor. Draw the
7.2.1 Membrane heterotrophic plate count, M-HPC Formu- residual fluid from the monitor.
lation: 8.1.9 Oneofthetwonutrientmedia(7.2.1and7.2
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