ASTM D6329-98(2015)
(Guide)Standard Guide for Developing Methodology for Evaluating the Ability of Indoor Materials to Support Microbial Growth Using Static Environmental Chambers
Standard Guide for Developing Methodology for Evaluating the Ability of Indoor Materials to Support Microbial Growth Using Static Environmental Chambers
SIGNIFICANCE AND USE
4.1 The static chambers have several different applications:
4.1.1 The static chambers can be used to compare the susceptibility of different materials to the colonization and amplification of various microorganisms under defined conditions.
4.1.2 Chambers operated at high relative humidities may be used to perform worst case scenario screening tests on materials by providing an atmosphere where environmental conditions may be favorable for microbial growth.
4.1.3 Use of multiple chambers with different environmental parameters, such as a range of relative humidities, permits the evaluation of multiple microenvironments and allows investigation of materials under differing environmental conditions.
4.1.4 Drying requirements for wetted materials may also be investigated. This information may be relevant for determining material resistance to microbial growth after becoming wet. These conditions may simulate those where materials are subjected to water incursion through leaks as well as during remediation of a building after a fire.
4.1.5 Growth rates of microorganisms on the material may also be investigated. Once it has been established that organisms are able to grow on a particular material under defined conditions, investigations into the rate of organism growth may be performed. These evaluations provide base line information and can be used to evaluate methods to limit or contain amplification of microorganisms.
4.2 These techniques should be performed by personnel with training in microbiology. The individual must be competent in the use of sterile technique, which is critical to exclude external contamination of materials.
SCOPE
1.1 Many different types of microorganisms (for example, bacteria, fungi, viruses, algae) can occupy indoor spaces. Materials that support microbial growth are potential indoor sources of biocontaminants (for example, spores and toxins) that can become airborne indoor biopollutants. This guide describes a simple, relatively cost effective approach to evaluating the ability of a variety of materials to support microbial growth using a small chamber method.
1.2 This guide is intended to assist groups in the development of specific test methods for a definite material or groups of materials.
1.3 Static chambers have certain limitations. Usually, only small samples of indoor materials can be evaluated. Care must be taken that these samples are representative of the materials being tested so that a true evaluation of the material is performed.
1.4 Static chambers provide controlled laboratory microenvironment conditions. These chambers are not intended to duplicate room conditions, and care must be taken when interpreting the results. Static chambers are not a substitute for dynamic chambers or field studies.
1.5 A variety of microorganisms, specifically bacteria and fungi, can be evaluated using these chambers. This guide is not intended to provide human health effect data. However, organisms of clinical interest, such as those described as potentially allergenic, may be studied using this approach.
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
General Information
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Designation: D6329 − 98 (Reapproved 2015)
Standard Guide for
Developing Methodology for Evaluating the Ability of Indoor
Materials to Support Microbial Growth Using Static
1
Environmental Chambers
This standard is issued under the fixed designation D6329; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope responsibility of the user of this standard to establish appro-
priate safety and health practices and determine the applica-
1.1 Many different types of microorganisms (for example,
bility of regulatory limitations prior to use.
bacteria, fungi, viruses, algae) can occupy indoor spaces.
Materials that support microbial growth are potential indoor
2. Referenced Documents
sources of biocontaminants (for example, spores and toxins)
2
2.1 ASTM Standards:
that can become airborne indoor biopollutants. This guide
describes a simple, relatively cost effective approach to evalu- D1193 Specification for Reagent Water
D1356 Terminology Relating to Sampling and Analysis of
ating the ability of a variety of materials to support microbial
growth using a small chamber method. Atmospheres
E104 Practice for Maintaining Constant Relative Humidity
1.2 This guide is intended to assist groups in the develop-
by Means of Aqueous Solutions
ment of specific test methods for a definite material or groups
3
2.2 APHA Standards:
of materials.
Standard Methods for the Examination of Water and Waste-
1.3 Static chambers have certain limitations. Usually, only
water
small samples of indoor materials can be evaluated. Care must
be taken that these samples are representative of the materials
3. Terminology
being tested so that a true evaluation of the material is
3.1 Definitions—For definitions of terms used in this guide,
performed.
refer to Terminology D1356.
1.4 Static chambers provide controlled laboratory microen-
3.2 Definitions of Terms Specific to This Standard:
vironment conditions. These chambers are not intended to
3.2.1 amplification—the act or result of increasing the
duplicate room conditions, and care must be taken when
quantity of microorganisms.
interpreting the results. Static chambers are not a substitute for
dynamic chambers or field studies.
3.2.2 CFU—colony forming unit, which may arise from a
single organism or multiple units, such as spores, in the case of
1.5 A variety of microorganisms, specifically bacteria and
the fungi.
fungi, can be evaluated using these chambers. This guide is not
intended to provide human health effect data. However, organ- 3.2.3 colony—macroscopically visible growth.
isms of clinical interest, such as those described as potentially
3.2.4 inoculation—the act of introducing a microorganism
allergenic, may be studied using this approach.
(inoculum) into the test material.
1.6 The values stated in SI units are to be regarded as
3.2.5 inoculum—viable test microorganism introduced onto
standard. No other units of measurement are included in this
a material by implanting a small amount on the surface or
standard.
substrate.
1.7 This standard does not purport to address all of the
3.2.6 plate—petri dish containing microbiological agar me-
safety concerns, if any, associated with its use. It is the
dia on which microorganism are grown.
1 2
This guide is under the jurisdiction of ASTM Committee D22 on Air For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Quality and is the direct responsibility of Subcommittee D22.08 on Sampling and contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Analysis of Mold. Standards volume information, refer to the standard’s Document Summary page on
Current edition approved July 1, 2015. Published July 2015. Originally approved the ASTM website.
3
in 1998. Last previous edition approved in 2008 as D6329 – 98 (2008). DOI: Available from American Public Health Association (APHA), 800 I St., NW,
10.1520/D6329-98R15. Washington, DC 20001, http://www.apha.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
D6329 − 98 (2015)
3.2.7 static chamber—a small chamber (enclosed space) static chamber. Acrylic desiccators are readily available, easily
with no internal forced air motion. adaptable, and relatively inexpensive. Other options, such as
glass, are also acceptable. Glass has the advantage of being
3.2.8 susceptibility—the vulnerability of a material or sur-
autoclavable; however, it is frequently much less portable. The
face to colonization by microorganisms.
chamber door must provide r
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D6329 − 98 (Reapproved 2008) D6329 − 98 (Reapproved 2015)
Standard Guide for
Developing Methodology for Evaluating the Ability of Indoor
Materials to Support Microbial Growth Using Static
1
Environmental Chambers
This standard is issued under the fixed designation D6329; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 Many different types of microorganisms (for example, bacteria, fungi, viruses, algae) can occupy indoor spaces. Materials
that support microbial growth are potential indoor sources of biocontaminants (for example, spores and toxins) that can become
airborne indoor biopollutants. This guide describes a simple, relatively cost effective approach to evaluating the ability of a variety
of materials to support microbial growth using a small chamber method.
1.2 This guide is intended to assist groups in the development of specific test methods for a definite material or groups of
materials.
1.3 Static chambers have certain limitations. Usually, only small samples of indoor materials can be evaluated. Care must be
taken that these samples are representative of the materials being tested so that a true evaluation of the material is performed.
1.4 Static chambers provide controlled laboratory microenvironment conditions. These chambers are not intended to duplicate
room conditions, and care must be taken when interpreting the results. Static chambers are not a substitute for dynamic chambers
or field studies.
1.5 A variety of microorganisms, specifically bacteria and fungi, can be evaluated using these chambers. This guide is not
intended to provide human health effect data. However, organisms of clinical interest, such as those described as potentially
allergenic, may be studied using this approach.
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
D1356 Terminology Relating to Sampling and Analysis of Atmospheres
E104 Practice for Maintaining Constant Relative Humidity by Means of Aqueous Solutions
3
2.2 APHA StandardsStandards: :
Standard Methods for the Examination of Water and Wastewater for the Examination of Water and Wastewater
3. Terminology
3.1 Definitions—For definitions of terms used in this guide, refer to Terminology D1356.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 amplification—the act or result of increasing the quantity of microorganisms.
1
This guide is under the jurisdiction of ASTM Committee D22 on Air Quality and is the direct responsibility of Subcommittee D22.08 on Indoor AirSampling and
Analysis of Mold.
Current edition approved April 1, 2008July 1, 2015. Published July 2008July 2015. Originally approved in 1998. Last previous edition approved in 20032008 as
D6329 - 98D6329 – 98 (2008).(2003). DOI: 10.1520/D6329-98R08.10.1520/D6329-98R15.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
th
3
Available from American Public Health Association, 1015 15Association (APHA), 800 I St., NW, Washington, DC 20036.20001, http://www.apha.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
D6329 − 98 (2015)
3.2.2 CFU—colony forming unit, which may arise from a single organism or multiple units, such as spores, in the case of the
fungi.
3.2.3 colony—macroscopically visible growth.
3.2.4 inoculation—the act of introducing a microorganism (inoculum) into the test material.
3.2.5 inoculum—viable test microorganism introduced onto a material by implanting a small amount on the surface or substrate.
3.2.6 plate—petri dish containing microbiological agar media on which microorganism are grown.
3.2.7 static chamber—a small cham
...
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