ASTM E1428-99(2004)
(Test Method)Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by Streptoverticillium reticulum (A Pink Stain Organism)
Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by <med><i>Streptoverticillium reticulum</i></i> (A Pink Stain Organism)
SIGNIFICANCE AND USE
This test method provides a technique for evaluating antimicrobials in or on polymeric solids against staining by Streptoverticillium reticulum, and should assist in the prediction of performance of treated articles under actual field conditions.
Conditioning of the specimens, such as exposure to leaching, weathering, and heat treatment, may have significant effects on performance of antimicrobials against staining. Determination of these effects is not included in this test method.
SCOPE
1.1 This test method is used for determining the performance of antimicrobial agents used in or on synthetic polymeric solids against staining by the actinomycete Streptoverticillium reticulum. This organism has been chosen as an indicator organism, although other organisms have been known to cause undesirable staining in polymeric solids.
1.2 This test method is not suitable for evaluating dark-pigmented test samples.
1.3 A knowledge of microbiological techniques is recommended for these procedures.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation:E1428–99 (Reapproved 2004)
Standard Test Method for
Evaluating the Performance of Antimicrobials in or on
Polymeric Solids Against Staining by Streptoverticillium
reticulum (A Pink Stain Organism)
This standard is issued under the fixed designation E1428; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4. Interferences
1.1 This test method is used for determining the perfor- 4.1 An interference may be caused by contamination of
mance of antimicrobial agents used in or on synthetic poly- plates and agar by unwanted organisms that settle from the
meric solids against staining by the actinomycete Streptover- environment.
ticillium reticulum. This organism has been chosen as an 4.2 Dark pigments mask observation of the pink stain.
indicatororganism,althoughotherorganismshavebeenknown
5. Apparatus
to cause undesirable staining in polymeric solids.
1.2 This test method is not suitable for evaluating dark- 5.1 Petri dishes, 100 mm diameter.
5.2 Cotton swabs, sterile.
pigmented test samples.
1.3 A knowledge of microbiological techniques is recom- 5.3 Incubator—Incubating equipment for this test method
shall maintain a temperature of 29 6 1°C.
mended for these procedures.
1.4 This standard does not purport to address all of the 5.4 Autoclave.
5.5 Sterilizer, ethylene oxide (optional).
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appro-
6. Reagents and Materials
priate safety and health practices and determine the applica-
6.1 Yeast Malt Extract Agar (ISP Medium 2) —Prepare this
bility of regulatory limitations prior to use.
medium according to manufacturer’s directions.
2. Summary of Test Method
6.2 Inoculum—Streptoverticillium reticulum ATCC
25607. Maintain stock cultures on yeast malt extract agar.The
2.1 Test specimens are placed on an agar surface inoculated
with Streptoverticillium reticulum and incubated.After incuba- stock may be kept for not more than 12 months at approxi-
mately 3 to 10°C. Subcultures, incubated at 29 6 1°C for 7 to
tion, test specimens are rated visually by percentage of sample
area stained. 14 days, shall be used for inoculation.
7. Test Specimens
3. Significance and Use
3.1 This test method provides a technique for evaluating 7.1 From each test unit, cut duplicate 0.75 in. diameter
discs. If the test unit is of different construction on each side,
antimicrobials in or on polymeric solids against staining by
Streptoverticillium reticulum, and should assist in the predic- two specimens of each, two face up and two face down, shall
be tested.
tion of performance of treated articles under actual field
conditions. 7.2 A test unit containing no biocide should be included as
a positive stain control.
3.2 Conditioning of the specimens, such as exposure to
leaching, weathering, and heat treatment, may have significant
effects on performance of antimicrobials against staining.
Presterilized and disposable plastic petri dishes are available from most
Determination of these effects is not included in this test
laboratory supply houses.
method.
The sole source of supply of Bacto-Yeast Malt ExtractAgar, Stock No. 0770-01
known to the committee at this time is Difco Labs, P.O. Box 1058A, Detroit, MI
28232. If you are aware of alternative suppliers, please provide this information to
This test method is under the jurisdiction of ASTM Committee E35 on ASTM International Headquarters. Your comments will receive careful consider-
Pesticides and Alternative Control Agents and is the direct responsibility of ation at a meeting of the responsible technical committee , which you may attend.
Subcommittee E35.15 on Antimicrobial Agents. American Type Culture Collection, 10801 University Blvd., Manassas, VA
Current edition approved Oct, 1, 2004. Published October 2004. Originally 20110.
approved in 1991. Last previous edition approved in 1999 as E1428 – 99. DOI: Atest unit is a solid in the form of plastic sheets, films, coated fabrics or similar
10.1520/E1428-99R04. polymeric materials.
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