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ASTM D7658-17

(Test Method)

Standard Test Method for Direct Microscopy of Fungal Structures from Tape

Standard Test Method for Direct Microscopy of Fungal Structures from Tape

SIGNIFICANCE AND USE
5.1 The significance of this test method is to standardize the analysis of the detection of removable fungal structures lifted from a surface with tape to improve consistency between laboratories and analysts.  
5.2 This test method is intended to ensure consistent data to the end user.  
5.3 Fungal structures are identified and semi-quantified regardless of whether they would or would not grow in culture.  
5.4 It must be emphasized that the detector in this test method is the analyst, and therefore results are subjective, depending on the experience, training, qualification, optical acuity, and mental fatigue of the analyst.  
5.5 This test method can be used to assess the presence and characteristics of fungal material on a surface.
SCOPE
1.1 This test method uses optical microscopy for the detection, semi-quantification, and identification of fungal structures in tape lift preparations.  
1.2 This test method describes the preparation techniques for tape-lift matrices, the procedure for confirming the presence of fungal structures, and the reporting of observed fungal structures  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Historical
Publication Date
28-Feb-2017
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
D22 - Air Quality
Drafting Committee
D22.08 - Assessment, Sampling, and Analysis of Microorganisms
Current Stage
Ref Project

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ASTM D7658-17 - Standard Test Method for Direct Microscopy of Fungal Structures from Tape
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D7658 − 17
Standard Test Method for
1
Direct Microscopy of Fungal Structures from Tape
This standard is issued under the fixed designation D7658; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.2.1 fungal structure (sing.), n—a collective term for a
fragment-orgroupsoffragmentsfromfungi,includingbutnot
1.1 This test method uses optical microscopy for the
limited to conidia, conidiophores, hyphae and spores.
detection, semi-quantification, and identification of fungal
3.2.2 magnification/resolution combination 1, n—
structures in tape lift preparations.
~100–400× total magnification and a point to point resolution
1.2 This test method describes the preparation techniques
of 0.7 µm or better.
for tape-lift matrices, the procedure for confirming the pres-
3.2.3 magnification/resolution combination 2, n— ~400× or
ence of fungal structures, and the reporting of observed fungal
greatertotalmagnificationandapointtopointresolutionof0.5
structures
µm or better.
1.3 The values stated in SI units are to be regarded as
3.2.4 mounting medium, n—a liquid, for example, lactic
standard. No other units of measurement are included in this
acid or prepared stain, used to immerse the sample particulate
standard.
matter and to attach a cover slip to the sample.
1.4 This standard does not purport to address all of the
3.2.5 tape lift sample, n—material lifted from a surface
safety concerns, if any, associated with its use. It is the
using clear, transparent, single sided, adhesive collection
responsibility of the user of this standard to establish appro-
medium, typically tape or commercially available prepared
priate safety and health practices and determine the applica-
slides.
bility of regulatory limitations prior to use.
1.5 This international standard was developed in accor-
4. Summary of Test Method
dance with internationally recognized principles on standard-
4.1 A tape lift sample is prepared.
ization established in the Decision on Principles for the
4.2 The prepared sample is examined on an optical micro-
Development of International Standards, Guides and Recom-
scope for the presence, type and semi-quantification of fungal
mendations issued by the World Trade Organization Technical
structures and reported.
Barriers to Trade (TBT) Committee.
5. Significance and Use
2. Referenced Documents
5.1 Thesignificanceofthistestmethodistostandardizethe
2
2.1 ASTM Standards:
analysis of the detection of removable fungal structures lifted
D1193Specification for Reagent Water
from a surface with tape to improve consistency between
D1356Terminology Relating to Sampling and Analysis of
laboratories and analysts.
Atmospheres
5.2 This test method is intended to ensure consistent data to
the end user.
3. Terminology
5.3 Fungal structures are identified and semi-quantified
3.1 Definitions—For definitions of other terms used in this
regardlessofwhethertheywouldorwouldnotgrowinculture.
test method, refer to Terminology D1356.
3.2 Definitions of Terms Specific to This Standard:
5.4 It must be emphasized that the detector in this test
method is the analyst, and therefore results are subjective,
depending on the experience, training, qualification, optical
1
This test method is under the jurisdiction of ASTM Committee D22 on Air
acuity, and mental fatigue of the analyst.
Quality and is the direct responsibility of Subcommittee D22.08 on Sampling and
5.5 This test method can be used to assess the presence and
Analysis of Mold.
Current edition approved March 1, 2017. Published April 2017. DOI: 10.1520/
characteristics of fungal material on a surface.
D7658-17.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or 6. Interferences
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
6.1 Look-Alike Non-Fungal Particles—Certaintypesofpar-
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. ticles of non-fungal origin may resemble fungal structures.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D7658 − 17
These particles and artifacts may include air or plant resin, thicknessaccordingtotherecommendationsofthemicroscope
bubbles, starch, talc or cosmetic particles, or combustion objective lens manufacturer.
products. Non-fungal reference slides (mounted similarly to
8.3 Microscope slides,glassslidestobeusedwhensamples
tape-liftsamples)shouldbeexaminedbylaboratoryanalyststo
are not taken on commercially available lift-samples.
...

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