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ASTM D8068-18

(Practice)

Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems

Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems

SIGNIFICANCE AND USE
4.1 This practice is intended for the collection of airborne particles on agar plates using inertial impaction for the purpose of culturing fungi or bacteria.  
4.2 This practice is valuable when species level identification or quantity of culturable aerosolized fungi and bacteria are important factors for the indoor air quality investigation.  
4.3 It is the responsibility of the user to assure that they are in compliance with all local, state, and federal regulations governing the inspection of buildings for fungal colonization and the collection of associated samples.  
4.4 This practice is intended to provide the user with a basic understanding of the equipment, materials, and instructions necessary to effectively collect air samples on agar plates using an inertial impactor.  
4.5 This practice is intended to minimize systematic sampling variations between different data sets.
SCOPE
1.1 The purpose of this practice is to describe procedures for the collection of culturable airborne fungal spores or fragments or bacteria on agar plates using inertial impaction sampling techniques.  
1.2 This practice does not include collection of culturable fungi or bacteria by devices not using agar plates.  
1.3 This practice presumes that the user has a fundamental understanding of field investigative techniques related to the scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory conditions for analysis.  
1.4 This practice does not address the development of a formal hypothesis or the establishment of appropriate and defensible investigation and sampling objectives. It is presumed the investigator has the experience and knowledge base to address these issues.  
1.5 This practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample collection. It is the user's responsibility to seek or obtain the information and knowledge necessary to interpret the sample results reported by the laboratory.  
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Historical
Publication Date
31-Oct-2018
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
D22 - Air Quality
Drafting Committee
D22.08 - Assessment, Sampling, and Analysis of Microorganisms
Current Stage
Ref Project

Relations

Replaced By
ASTM D8068-19 - Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems
Effective Date
01-Mar-2019

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ASTM D8068-18 - Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction SystemsASTM D8068-18 - Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems
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ASTM D8068-18 - Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems
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Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D8068 − 18
Standard Practice for
Collection of Culturable Airborne Fungi or Bacteria on Agar
1
Plates by Inertial Impaction Systems
This standard is issued under the fixed designation D8068; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
This practice was introduced to bring uniformity to the process of sample collection for culturable
fungi or bacteria using inertial impaction samplers for analysis by culture and optical microscopy.
1. Scope priate safety, health, and environmental practices and deter-
mine the applicability of regulatory limitations prior to use.
1.1 Thepurposeofthispracticeistodescribeproceduresfor
1.8 This international standard was developed in accor-
the collection of culturable airborne fungal spores or fragments
dance with internationally recognized principles on standard-
or bacteria on agar plates using inertial impaction sampling
ization established in the Decision on Principles for the
techniques.
Development of International Standards, Guides and Recom-
1.2 This practice does not include collection of culturable
mendations issued by the World Trade Organization Technical
fungi or bacteria by devices not using agar plates.
Barriers to Trade (TBT) Committee.
1.3 This practice presumes that the user has a fundamental
2. Referenced Documents
understanding of field investigative techniques related to the
2
2.1 ASTM Standards:
scientific process, and sampling plan development and imple-
mentation. It is important to establish the related hypothesis to D1356 Terminology Relating to Sampling and Analysis of
Atmospheres
be tested and the supporting analytical methodology needed in
order to identify the sampling media to be used and the D6044 Guide for Representative Sampling for Management
of Waste and Contaminated Media
laboratory conditions for analysis.
D3195/D3195M Practice for Rotameter Calibration
1.4 This practice does not address the development of a
E2589 Terminology Relating to Nonsieving Methods of
formal hypothesis or the establishment of appropriate and
Powder Characterization
defensible investigation and sampling objectives. It is pre-
F1671 Test Method for Resistance of Materials Used in
sumed the investigator has the experience and knowledge base
Protective Clothing to Penetration by Blood-Borne Patho-
to address these issues.
gens Using Phi-X174 Bacteriophage Penetration as a Test
1.5 This practice does not provide the user sufficient infor-
System
mation to allow for interpretation of the analytical results from
3. Terminology
sample collection. It is the user’s responsibility to seek or
obtain the information and knowledge necessary to interpret
3.1 Definitions—For definitions of terms used in this
the sample results reported by the laboratory.
practice, see Terminology D1356.
3.1.1 agar, n—a semisolid culture medium used to support
1.6 The values stated in SI units are to be regarded as
the growth of bacteria and other micro-organisms. F1671
standard. No other units of measurement are included in this
standard.
3.1.2 sample, n—a portion of a population.
3.1.2.1 Discussion—A portion of material that is taken for
1.7 This standard does not purport to address all of the
testing or record purposes. D6044
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appro-
3.1.3 sample, representative, n—a sample collected in such
a manner that it reflects the characteristics of interest of a
1
This practice is under the jurisdiction ofASTM Committee D22 on Air Quality
2
and is the direct responsibility of Subcommittee D22.08 on Assessment, Sampling, For referenced ASTM standards, visit the ASTM website, www.astm.org, or
and Analysis of Microorganisms. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Current edition approved Nov. 1, 2018. Published November 2018. DOI: Standards volume information, refer to the standard’s Document Summary page on
10.1520/D8068-18. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D8068 − 18
population (as defined) from which it is collected. 4.4 This practice is intended to provide the user with a basic
adapted from D6044 understanding of the equipment, materials, and instructions
3.1.3.1 Discussion—A population in the context of this necessarytoeffectivelycollectairsamplesonagarplatesusing
standard refers to culturable fungi- or bacteria colony-forming an inertial impactor.
units that exi
...

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1.1 Many different types of microorganisms (for example, bacteria, fungi, viruses, algae) can occupy indoor spaces. Materials that support microbial growth are potential indoor sources of biocontaminants (for example, spores and toxins) that can become airborne indoor biopollutants. This guide describes a simple, relatively cost effective approach to evaluating the ability of a variety of materials to support microbial growth using a small chamber method.  
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1.1 The purpose of this practice is to describe the procedures for collection of surface samples using sterile swabs.  
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SIGNIFICANCE AND USE
5.1 The significance of this test method is to standardize the analysis of the detection of removable fungal structures lifted from a surface with tape to improve consistency between laboratories and analysts.  
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SCOPE
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4.1 This practice is intended for the collection of airborne particles on agar plates using inertial impaction for the purpose of culturing fungi or bacteria.  
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1.1 This practice describes a direct microscopic counting method (DMC) for the enumeration of the acid-fast stained mycobacteria population in metalworking fluids. It can be used to detect levels of total mycobacteria population, including culturable as well as non-culturable (possibly dead or moribund) bacterial cells. This practice is recommended for all water-based metalworking fluids (Classification D2881).  
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4.1 This test method enumerates salt tolerant (halophilic) bacteria, and proteolytic bacteria that are also salt tolerant. Under the conditions of this test method those bacteria are equated as halophilic organisms. Salt tolerant proteolytic bacteria have been known to cause damage to hides and skins in raceway brine.
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Standard Test Method for Enumeration of Proteolytic Bacteria in Fresh (Uncured) Hides and Skins

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4.1 This test method enumerates proteolytic bacteria. Proteolytic bacteria have been known to cause damage to hides and skins.
SCOPE
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1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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Standard Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity In Carpets

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