ASTM D4783-01e1
(Test Method)Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi
Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi
SCOPE
1.1 These test methods cover the determination of the resistance of liquid adhesive preparations to microbial attack in the container by challenging adhesive specimens with cultures of bacteria, yeast, or fungi, and checking for their ability to return to sterility. These test methods return qualitative results.
1.2 The values stated in SI units are to be regarded as the standard. The values in parentheses are for information only.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological technique. Specific precautionary statements are given in Section 8.
General Information
Relations
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
e1
Designation:D4783–01
Standard Test Methods for
Resistance of Adhesive Preparations in Container to Attack
1
by Bacteria, Yeast, and Fungi
This standard is issued under the fixed designation D 4783; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1
e NOTE—The ATCC for Gliocladium roseum in X3.1.5 was corrected editorially in January 2007.
Committee E35 on Pesticides. The procedure in this method outlines a
1. Scope*
serial dilution method of determining plate count using a pour plate
1.1 These test methods cover the determination of the
technique.
resistanceofliquidadhesivepreparationstomicrobialattackin
2.2 TAPPI Method:
the container by challenging adhesive specimens with cultures
4
T 487 Fungus Resistance of Paper and Paperboard
of bacteria, yeast, or fungi, and checking for their ability to
2.3 CSMA:
return to sterility. These test methods return qualitative results.
5
Cosmetics Preservation, Method 38
1.2 The values stated in SI units are to be regarded as the
standard. The values in parentheses are for information only.
3. Terminology
1.3 This standard does not purport to address all of the
3.1 Definitions—Many terms in these test methods are
safety concerns, if any, associated with its use. It is the
defined in Terminology D 907.
responsibility of the user of this standard to establish appro-
3.2 Definitions of Terms Specific to This Standard:
priate safety and health practices and determine the applica-
3.2.1 adhesive preparation—the adhesive as packaged for
bility of regulatory limitations prior to use. These test methods
distribution, storage, and use.
are designed to be used by persons trained in correct micro-
3.3 Abbreviations:
biological technique. Specific precautionary statements are
3.3.1 PBS—phosphate buffered saline.
given in Section 8.
3.3.2 PDA—potato dextrose agar.
2. Referenced Documents 3.3.3 YMPG—yeast malt peptone glucose (agar).
2
2.1 ASTM Standards:
4. Summary of Test Methods
D 907 Terminology of Adhesives
4.1 The adhesive specimen is challenged by inoculation
D 4299 Test Method for Effect of Bacterial Contamination
with a culture of bacteria, yeast, or fungi, which may be a
on Performance of Adhesive Preparations and Adhesive
3 single species or a mixed culture of several species, following
Films
the guidelines given in Note 6. The inoculated adhesive
D 4300 Test Methods for Ability of Adhesive Films to
specimenisstoredat21to27°C(70to80°F)for7days,during
Support or Resist the Growth of Fungi
which time cultures (streak plates) are made at preset intervals.
E 640 Test Method for Preservatives in Water-Containing
See Note 2. At any point in the series of challenges, if the
Cosmetics
inoculated specimen shows microbial growth on the streak
NOTE 1—Test Method E 640 is under the jurisdiction of ASTM
plates made during the week following the challenge (indicat-
ing that it has not returned to sterility), the test is discontinued,
1
and the sample is reported as not resistant to attack in the
These test methods are under the jurisdiction of ASTM Committee D14 on
Adhesives and are the direct responsibility of Subcommittee D14.30 on Wood container by the species or combination of species used as the
Adhesives.
inoculum. If the cultures show no growth, the test is repeated
Current edition approved Oct. 10, 2001. Published December 2001. Originally
with up to four challenges. If the specimen tests out as sterile
published as D 4783 – 88. Last previous edition D 4783 – 98a.
2
following the fourth challenge, it is reported to be resistant to
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
4
the ASTM website. Available from TAPPI, P.O. Box 105113, Atlanta, GA 30348.
3 5
Withdrawn. This method is the same as Test Method E 640.
*A Summary of Changes section appears at the end of this standard.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1
---------------------- Page: 1 ----------------------
e1
D4783–01
viable organisms or the reduction in population with increasing levels of
attackinthecontainerbythespeciesorcombinationofspecies
biocide. Letheen broth is recommended for the diluent and Letheen agar
of bacteria, fungi, or yeast used as the inoculum. At the
for the pour plate. See Note 1.
discretion of the biological laboratory, the test may be discon-
tinued
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.