ASTM D7789-12(2021)
(Practice)Standard Practice for Collection of Fungal Material from Surfaces by Swab
Standard Practice for Collection of Fungal Material from Surfaces by Swab
SIGNIFICANCE AND USE
4.1 This practice provides a procedure for collecting surface material using a sterile swab.
4.2 A swab sample collected according to this practice is intended to be used to assess fungal material on surfaces.
4.3 A swab sample collected from an area with defined dimensions can be used to quantify fungal material per unit area or for qualitative analysis.
4.4 A swab sample collected from point(s) of interest can be used for qualitative analysis or to quantify fungal material per sample.
4.5 A swab sample collected according to this practice can be analyzed by direct microscopy, culture, or biochemical analysis.
SCOPE
1.1 The purpose of this practice is to describe the procedures for collection of surface samples using sterile swabs.
1.2 The purpose of this practice is to support the field investigator in differentiating fungal materials from non-fungal material such as scuffs, soot deposits, stains, pigments, dust, efflorescence, adhesives, dust, and water stains.
1.3 This practice does not address building occupant exposures, or occupant health risks.
1.4 The samples collected by this practice are appropriate for culture, direct microscopy, and biochemical analysis, or combination thereof.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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Designation: D7789 − 12 (Reapproved 2021)
Standard Practice for
Collection of Fungal Material from Surfaces by Swab
This standard is issued under the fixed designation D7789; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.1 sample, n—a portion of a population intended to be
representativeofthewhole.aportionofapopulation.Aportion
1.1 The purpose of this practice is to describe the proce-
of material that is taken for testing or record purposes. D6044
dures for collection of surface samples using sterile swabs.
3.1.2 sampling, n—aprocessconsistingofthewithdrawalor
1.2 The purpose of this practice is to support the field
isolation of a fractional part of the whole. D1356
investigator in differentiating fungal materials from non-fungal
3.2 Definitions of Terms Specific to This Standard:
material such as scuffs, soot deposits, stains, pigments, dust,
3.2.1 area (surface, sampled), n—a defined, measured ex-
efflorescence, adhesives, dust, and water stains.
tent of surface sampled.
1.3 This practice does not address building occupant
3.2.2 biochemical analysis, n—act of assessing chemical
exposures, or occupant health risks.
substances (that is, chitin, enzyme activity, ergosterol,
1.4 The samples collected by this practice are appropriate
mycotoxin, DNA) originating from living or dead organisms.
for culture, direct microscopy, and biochemical analysis, or
3.2.3 chain of custody (COC), n—a document that provides
combination thereof.
for the traceable transfer of field samples to the analytical
1.5 This standard does not purport to address all of the
laboratory. It may or may not be combined with the field data
safety concerns, if any, associated with its use. It is the
sheet.
responsibility of the user of this standard to establish appro-
3.2.4 culture analysis, n—act of assessing microorganisms
priate safety, health, and environmental practices and deter-
(if present) capable of growing on selected media under
mine the applicability of regulatory limitations prior to use.
artificial and controlled conditions.
1.6 This international standard was developed in accor-
dance with internationally recognized principles on standard- 3.2.5 direct microscopy analysis, n—act of assessing micro-
ization established in the Decision on Principles for the organisms (if present) using an optical compound microscope.
Development of International Standards, Guides and Recom-
3.2.6 field data sheet, n—a record of varying names that
mendations issued by the World Trade Organization Technical
provides a reference document for information directly related
Barriers to Trade (TBT) Committee.
to the sample collection event, including pre- and post-
calibration data.
2. Referenced Documents
3.2.7 fungal material, n—fungal spores, hyphae and repro-
2.1 ASTM Standards:
ductive structures.
D1356 Terminology Relating to Sampling and Analysis of
3.2.8 fungi, n—eukaryotic, heterotrophic, absorptive organ-
Atmospheres
isms that develop a rather diffuse, branched, tubular body (that
D6044 Guide for Representative Sampling for Management
is, network of hyphae) and reproduce by means of spores. The
of Waste and Contaminated Media
terms ‘mold’ and ‘mildew’ are frequently used by laypersons
when referring to various fungal colonization. For purposes of
3. Terminology
this practice, these terms have no technical meaning.
3.1 Definitions:
4. Significance and Use
This practice is under the jurisdiction ofASTM Committee D22 on Air Quality
4.1 This practice provides a procedure for collecting surface
and is the direct responsibility of Subcommittee D22.08 on Assessment, Sampling,
material using a sterile swab.
and Analysis of Microorganisms.
Current edition approved July 1, 2021. Published July 2021. Originally approved
4.2 A swab sample collected according to this practice is
in 2012. Last previous edition approved in 2012 as D7789 – 12. DOI: 10.1520/
intended to be used to assess fungal material on surfaces.
D7789-12R21.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. Kendrick, B., The Fifth Kingdom, 3rd Edition, 1992.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
D7789 − 12 (2021)
4.3 A swab sample collected from an area with defined 6.7.1 At a minimum, the sample field data sheet shall
dimensions can be used to quantify fungal material per unit contain the following information:
area or for qualitative analysis. 6.7.1.1 Sampling date;
6.7.1.2 Project name;
4.4 Aswab sample collected from point(s) of interest can be
6.7.1.3 Investigator’s name(s);
used for qualit
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D7789 − 12 D7789 − 12 (Reapproved 2021)
Standard Practice for
Collection of Fungal Material from Surfaces by Swab
This standard is issued under the fixed designation D7789; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 The purpose of this standard practice is to describe the procedures for collection of surface samples using sterile swabs.
1.2 The purpose of this standard practice is to support the field investigator in differentiating fungal materials from non-fungal
material such as scuffs, soot deposits, stains, pigments, dust, efflorescence, adhesives, dust, and water stains.
1.3 This practice does not address building occupant exposures, or occupant health risks.
1.4 The samples collected by this practice are appropriate for culture, direct microscopy, and/or biochemical analysis.and
biochemical analysis, or combination thereof.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and determine the
applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2.1 ASTM Standards:
D1356 Terminology Relating to Sampling and Analysis of Atmospheres
D6044 Guide for Representative Sampling for Management of Waste and Contaminated Media
3. Terminology
3.1 Definitions:
3.1.1 sample, n—a portion of a population intended to be representative of the whole. a portion of a population. A portion of
material that is taken for testing or record purposes. D6044
3.1.2 sampling, n—a process consisting of the withdrawal or isolation of a fractional part of the whole. D1356
3.2 Definitions of Terms Specific to This Standard:
This practice is under the jurisdiction of ASTM Committee D22 on Air Quality and is the direct responsibility of Subcommittee D22.08 on Sampling Assessment,
Sampling, and Analysis of MoldMicroorganisms.
Current edition approved Aug. 15, 2012July 1, 2021. Published November 2012July 2021. Originally approved in 2012. Last previous edition approved in 2012 as D7789
– 12. DOI: 10.1520/D7789-12.10.1520/D7789-12R21.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’sstandard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
D7789 − 12 (2021)
3.2.1 area (surface, sampled), n—a defined, measured extent of surface sampled.
3.2.2 biochemical analysis, n—act of assessing chemical substances (that is, chitin, enzyme activity, ergosterol, mycotoxin, DNA)
originating from living or dead organisms.
3.2.3 chain of custody (COC), n—a document that provides for the traceable transfer of field samples to the analytical laboratory.
It may or may not be combined with the Field Data Sheet.field data sheet.
3.2.4 culture analysis, n—act of assessing microorganisms (if present) capable of growing on selected media under artificial and
controlled conditions.
3.2.5 direct microscopy analysis, n—act of assessing microorganisms (if present) using an optical compound microscope.
3.2.6 field data sheet, n—a record of varying names that provides a reference document for information directly related to the
sample collection event, including pre- and post-calibration data.
3.2.7 fungal material, n—fungal spores, hyphae and reproductive structures.
3.2.8 fungi, n—eukaryotic, heterotrophic, absorptive organisms that develop a rather diffuse, branched, tubular body (that is,
network of hyphae) and reproduce by means of spores. The terms ‘mold’ and ‘mildew’ are frequently used by laypersons when
referring to various fungal colonization. For purposes of this standard,practice, these terms have no technical meaning.
3.2.8 fungal material, n—fungal spores, hyphae and reproductive structures.
4. Significance and Use
4.1 This practice provides a procedure for collecting surface material using a sterile swab.
4.2 A swab sample collected according to this practice is intended to be used to assess fungal material on surfaces.
4.3 A swab sample collected from an area with defined dimensions can be used to quantify fungal material per unit area or for
qualitative analysis.
4.4 A swab sample collected from point(s) of interest can be used for qualitative analysis or to quantify fungal material per sample.
4.5 A swab sample collected according to this standard
...
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