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ASTM D7789-12

(Practice)

Standard Practice for Collection of Fungal Material from Surfaces by Swab

Standard Practice for Collection of Fungal Material from Surfaces by Swab

SIGNIFICANCE AND USE
4.1 This practice provides a procedure for collecting surface material using a sterile swab.  
4.2 A swab sample collected according to this practice is intended to be used to assess fungal material on surfaces.  
4.3 A swab sample collected from an area with defined dimensions can be used to quantify fungal material per unit area or for qualitative analysis.  
4.4 A swab sample collected from point(s) of interest can be used for qualitative analysis or to quantify fungal material per sample.  
4.5 A swab sample collected according to this standard practice can be analyzed by direct microscopy, culture, or biochemical analysis.
SCOPE
1.1 The purpose of this standard practice is to describe the procedures for collection of surface samples using sterile swabs.  
1.2 The purpose of this standard practice is to support the field investigator in differentiating fungal materials from non-fungal material such as scuffs, soot deposits, stains, pigments, dust, efflorescence, adhesives, dust, and water stains.  
1.3 This practice does not address building occupant exposures, or occupant health risks.  
1.4 The samples collected by this practice are appropriate for culture, direct microscopy, and/or biochemical analysis.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
14-Aug-2012
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
D22 - Air Quality
Drafting Committee
D22.08 - Assessment, Sampling, and Analysis of Microorganisms
Current Stage
Ref Project

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ASTM D7789-12 - Standard Practice for Collection of Fungal Material from Surfaces by Swab
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D7789 − 12
Standard Practice for
1
Collection of Fungal Material from Surfaces by Swab
This standard is issued under the fixed designation D7789; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.1 sample, n—a portion of a population intended to be
representativeofthewhole.aportionofapopulation.Aportion
1.1 The purpose of this standard practice is to describe the
of material that is taken for testing or record purposes. D6044
procedures for collection of surface samples using sterile
3.1.2 sampling, n—aprocessconsistingofthewithdrawalor
swabs.
isolation of a fractional part of the whole. D1356
1.2 The purpose of this standard practice is to support the
3.2 Definitions of Terms Specific to This Standard:
field investigator in differentiating fungal materials from non-
3.2.1 area (surface, sampled), n—a defined, measured ex-
fungal material such as scuffs, soot deposits, stains, pigments,
tent of surface sampled.
dust, efflorescence, adhesives, dust, and water stains.
3.2.2 biochemical analysis, n—act of assessing chemical
1.3 This practice does not address building occupant
substances (that is, chitin, enzyme activity, ergosterol,
exposures, or occupant health risks.
mycotoxin, DNA) originating from living or dead organisms.
1.4 The samples collected by this practice are appropriate
3.2.3 chain of custody (COC), n—a document that provides
for culture, direct microscopy, and/or biochemical analysis.
for the traceable transfer of field samples to the analytical
1.5 This standard does not purport to address all of the
laboratory. It may or may not be combined with the Field Data
safety concerns, if any, associated with its use. It is the
Sheet.
responsibility of the user of this standard to establish appro-
3.2.4 culture analysis, n—act of assessing microorganisms
priate safety and health practices and determine the applica-
(if present) capable of growing on selected media under
bility of regulatory limitations prior to use.
artificial and controlled conditions.
1.6 This international standard was developed in accor-
dance with internationally recognized principles on standard- 3.2.5 direct microscopy analysis, n—act of assessing micro-
ization established in the Decision on Principles for the organisms (if present) using an optical compound microscope.
Development of International Standards, Guides and Recom-
3.2.6 field data sheet, n—a record of varying names that
mendations issued by the World Trade Organization Technical
provides a reference document for information directly related
Barriers to Trade (TBT) Committee.
to the sample collection event, including pre- and post-
calibration data.
2. Referenced Documents
3.2.7 fungi, n—eukaryotic, heterotrophic, absorptive organ-
2
2.1 ASTM Standards:
isms that develop a rather diffuse, branched, tubular body (that
3
D1356 Terminology Relating to Sampling and Analysis of
is, network of hyphae) and reproduce by means of spores. The
Atmospheres
terms ‘mold’ and ‘mildew’ are frequently used by laypersons
D6044 Guide for Representative Sampling for Management
when referring to various fungal colonization. For purposes of
of Waste and Contaminated Media
this standard, these terms have no technical meaning.
3.2.8 fungal material, n—fungal spores, hyphae and repro-
3. Terminology
ductive structures.
3.1 Definitions:
4. Significance and Use
4.1 This practice provides a procedure for collecting surface
1
This practice is under the jurisdiction ofASTM Committee D22 on Air Quality
material using a sterile swab.
and is the direct responsibility of Subcommittee D22.08 on Sampling and Analysis
of Mold.
4.2 A swab sample collected according to this practice is
Current edition approved Aug. 15, 2012. Published November 2012. DOI:
intended to be used to assess fungal material on surfaces.
10.1520/D7789-12.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
3
the ASTM website. Kendrick, Bryce, The Fifth Kingdom, 1992, 3rd Edition.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D7789 − 12
4.3 A swab sample collected from an area with defined 6.7.1 At a minimum, the sample field data sheet shall
dimensions can be used to quantify fungal material per unit contain the following information:
area or for qualitative analysis. 6.7.1.1 Sampling date;
6.7.1.2 Project
...

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Standard Practice for Collection of Fungal Material from Surfaces by Swab

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4.1 This practice provides a procedure for collecting surface material using a sterile swab.  
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4.3 A swab sample collected from an area with defined dimensions can be used to quantify fungal material per unit area or for qualitative analysis.  
4.4 A swab sample collected from point(s) of interest can be used for qualitative analysis or to quantify fungal material per sample.  
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