EN ISO 12966-3:2016

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH) (ISO 12966-3:2016)

ISO 12966-3:2016 specifies a rapid base-catalysed transesterification method for fats and oils with trimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils, but excluding those coming from milk and milk products. Isomerization of unsaturated fatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit. As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA).
Only about 70 % to 80 % of the free fatty acids are esterified. In the case of conjugated cyclopropyl and cyclopropenyl fatty acids, side reactions may occur, but these do not interfere with the determination of the fatty acids.

Tierische und pflanzliche Fette und Öle - Gaschromatographie von Fettsäuremethylestern - Teil 3: Herstellung von Methylestern mittels Trimethylsulfoniumhydroxid (TMSH) (ISO 12966-3:2016)

Dieser Teil der Internationalen Norm ISO 12966 legt ein Schnellverfahren zur basekatalysierten Umesterung von Fetten und Ölen mit Trimethylsufoniumhydroxid (TMSH) für die Herstellung von Fettsäuremethylestern fest. Das Verfahren ist ausschließlich auf die Herstellung von Methylestern von Fetten oder Ölen für die Gas-Flüssigkeit-Chromatographie (GLC) Analyse anwendbar. Es ist bei allen Fetten und Ölen anwendbar, außer bei Fetten aus Milch und Milcherzeugnissen. Eine Isomerisierung von ungesättigten Fettsäuren tritt nur in geringem Umfang auf und die Menge der vor¬handenen isomerisierten Fettsäuren liegt bei der Bestimmungsgrenze. Da eine Isomerisierung stattfindet, wird das Verfahren nicht für konjugierte Linolsäure (en: conjugated linoleic acid, CLA) empfohlen.
Freie Fettsäuren werden nur zu etwa 70 % bis 80 % verestert. Bei konjugierten Cyclopropyl- und Cyclopropenyl-Fettsäuren treten möglicherweise Nebenreaktionen auf, die sich jedoch nicht störend auf die Bestimmung der Fettsäuren auswirken.
ANMERKUNG   Dieser Teil von ISO 12966 beruht auf der Deutschen Einheitsmethode C VI 11e (98) (siehe Literaturhinweis [8]).

Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des esters méthyliques d'acides gras - Partie 3: Préparation des esters méthyliques à l'aide d'hydroxyde de triméthylsulfonium (TMSH) (ISO 12966-3:2016)

ISO 12966-3:2016 spécifie une méthode de transestérification rapide des corps gras par catalyse basique avec de l'hydroxyde de triméthylsulfonium (TMSH), pour préparer des esters méthyliques d'acides gras. Cette méthode s'applique exclusivement à la préparation des esters méthyliques des corps gras pour l'analyse chromatographique en phase gazeuse-liquide (CPG). Elle s'applique à tous les corps gras, à l'exclusion de ceux issus du lait et des produits laitiers. L'isomérisation des acides gras insaturés ne se produit que rarement et les acides gras isomérisés ne sont présents que dans la limite de détermination. Puisqu'il y a isomérisation, ce mode opératoire n'est pas recommandé pour l'acide linoléique conjugué (CLA).
Seulement environ 70 % à 80 % des acides gras libres sont estérifiés. Dans le cas d'acides gras conjugués cyclopropyl et cyclopropényl, des réactions secondaires peuvent se produire mais n'interfèrent pas dans la détermination des acides gras.

Živalske in rastlinske maščobe in olja - Plinska kromatografija metilnih estrov maščobnih kislin - 3. del: Priprava metilnih estrov s TMSH (trimethylsulfonium hydroxide) (ISO 12966-3:2016)

Ta del standarda ISO 12966 določa metodo hitre transesterifikacije, ki jo katalizirajo baze, za maščobe in olja s trimetilsulfonijevim hidroksidom (TMSH) za pripravo metilnih estrov maščobnih kislin. Metoda se uporablja izključno za pripravo metilnih estrov maščob in olj za analizo plinsko-tekočinske kromatografije (GLC). Uporablja se za vse maščobe in olja, razen tistih iz mleka in mlečnih izdelkov.
Do izomerizacije nenasičenih maščobnih kislin pride v majhnem obsegu in izomerizirane maščobne kisline so prisotne le na meji določitve. Ko se začne izomerizacija, postopek ni priporočljiv za konjugirano linolno kislino (CLA).
Esterificiranih je le približno 70 do 80 % prostih maščobnih kislin. V primeru konjugiranih ciklopropilnih in ciklopropenilnih maščobnih kislin lahko pride do stranskih reakcij, ki ne vplivajo na določevanje maščobnih kislin.
OPOMBA: ta del standarda ISO 12966 temelji na nemški standardni metodi C-VI 11e (98) (glej sklic [8]).

General Information

• Status: Published
• Publication Date: 07-Jun-2016
• Withdrawal Date: 30-Dec-2016
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 08-Jun-2016
• Completion Date: 08-Jun-2016
• Ref Project: SIST EN ISO 12966-3:2016 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH) (ISO 12966-3:2016)

Relations

• Replaces: EN ISO 12966-3:2009 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH) (ISO 12966-3:2009)
• Effective Date: 15-Jun-2016

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2016
1DGRPHãþD
SIST EN ISO 12966-3:2010
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD3OLQVNDNURPDWRJUDILMDPHWLOQLKHVWURY
PDãþREQLKNLVOLQGHO3ULSUDYDPHWLOQLKHVWURYV706+WULPHWK\OVXOIRQLXP
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Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters -
Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH) (ISO
12966-3:2016)
Tierische und pflanzliche Fette und Öle - Gaschromatographie von
Fettsäuremethylestern - Teil 3: Herstellung von Methylestern mittels
Trimethylsulfoniumhydroxid (TMSH) (ISO 12966-3:2016)
Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des
esters méthyliques d'acides gras - Partie 3: Préparation des esters méthyliques à l'aide
d'hydroxyde de triméthylsulfonium (TMSH) (ISO 12966-3:2016)
Ta slovenski standard je istoveten z: EN ISO 12966-3:2016
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 12966-3
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2016
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 12966-3:2009
English Version
Animal and vegetable fats and oils - Gas chromatography
of fatty acid methyl esters - Part 3: Preparation of methyl
esters using trimethylsulfonium hydroxide (TMSH) (ISO
12966-3:2016)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle -
Chromatographie en phase gazeuse des esters Gaschromatographie von Fettsäuremethylestern - Teil
méthyliques d'acides gras - Partie 3: Préparation des 3: Herstellung von Methylestern mittels
esters méthyliques à l'aide d'hydroxyde de Trimethylsulfoniumhydroxid (TMSH) (ISO 12966-
triméthylsulfonium (TMSH) (ISO 12966-3:2016) 3:2016)
This European Standard was approved by CEN on 12 May 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12966-3:2016 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
European foreword
This document (EN ISO 12966-3:2016) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2016, and conflicting national standards
shall be withdrawn at the latest by December 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 12966-3:2009.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 12966-3:2016 has been approved by CEN as EN ISO 12966-3:2016 without any
modification.
INTERNATIONAL ISO
STANDARD 12966-3
Second edition
2016-05-15
Animal and vegetable fats and oils —
Gas chromatography of fatty acid
methyl esters —
Part 3:
Preparation of methyl esters using
trimethylsulfonium hydroxide (TMSH)
Corps gras d’origines animale et végétale — Chromatographie en
phase gazeuse des esters méthyliques d’acides gras —
Partie 3: Préparation des esters méthyliques à l’aide d’hydroxyde de
triméthylsulfonium (TMSH)
Reference number
ISO 12966-3:2016(E)
©
ISO 2016
ISO 12966-3:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
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ii © ISO 2016 – All rights reserved

ISO 12966-3:2016(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Principle . 1
4 Reagents . 1
5 Apparatus . 2
6 Sampling . 2
7 Preparation of the test sample . 2
8 Procedure. 2
9 Test report . 3
Bibliography . 4
ISO 12966-3:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Elect
...