EN 49-2:2005
(Main)Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method)
Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method)
This document specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has been treated previously by full impregnation. This method is applicable to:
¾ water-insoluble chemicals which are being studied as active insecticides;
¾ organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;,
¾ organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, and
¾ water-soluble materials, for example salts.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven - Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Dieses Dokument legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens der Larven in einem zuvor durch Volltränkung behandelten Holz fest. Das Verfahren gilt für:
- wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
- organische Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- organische wasserdispergierbare Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
- wasserlösliche Stoffe, zum Beispiel Salze.
ANMERKUNG Das Verfahren kann in Verbindung mit einer geeigneten Alterungsbeanspruchung, zum Beispiel nach EN 73, angewendet werden.
Produits de préservation du bois - Détermination de l'efficacité protectrice vis à vis de Anobium punctatum (De Geer) par l'observation de la ponte et de la survie des larves - Partie 2 : Application par imprégnation (Méthode de laboratoire)
La présente partie de l'EN 49 prescrit une méthode de détermination de l'efficacité protectrice ou du seuil d'efficacité d'un produit de préservation du bois vis-à-vis d'Anobiurn punctatum (De Geer) par observation de la ponte et de la survie des larves dans du bois traité auparavant par imprégnation totale. La présente méthode est applicable :
¾ aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides, ou
¾ aux formules organiques telles qu'elles sont fournies ou préparées au laboratoire à partir de concentrés, ou
¾ aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées au laboratoire à partir de concentrés, ou
¾ aux produits solubles dans l'eau, par exemple aux sels.
NOTE Cette méthode peut être utilisée conjointement avec une épreuve de vieillisse ment, par exemple EN 73.
Zaščitna sredstva za les - Ugotavljanje učinkovitosti preventivne zaščite proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število položenih jajčec in preživelih ličink - 2. del: Postopek z globinsko impregnacijo lesa (Laboratorijska metoda)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2005
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SIST EN 49-2:1995
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QDYDGQHPXWUGRJODYFX$QRELXPSXQFWDWXP 'H*HHU JOHGHQDãWHYLORSRORåHQLK
MDMþHFLQSUHåLYHOLKOLþLQNGHO 3RVWRSHN]JORELQVNRLPSUHJQDFLMROHVD
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Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis a vis de
Anobium punctatum (De Geer) par l'observation de la ponte et de la survie des larves -
Partie 2 : Application par imprégnation (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 49-2:2005
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN 49-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2005
ICS 71.100.50 Supersedes EN 49-2:1992
English version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by egg-
laying and larval survival - Part 2: Application by impregnation
(Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden Wirkung
l'efficacité protectrice vis à vis de Anobium punctatum (De gegenüber Anobium punctatum (De Geer) durch
Geer) par l'observation de la ponte et de la survie des Beobachten der Eiablage und des Überlebens von Larven -
larves - Partie 2: Application par imprégnation (Méthode de Teil 2: Anwendung durch Volltränkung (Laborverfahren)
laboratoire)
This European Standard was approved by CEN on 3 February 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 49-2:2005: E
worldwide for CEN national Members.
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative reference .5
3 Terms and definitions .5
4 Principle.5
5 Test materials.6
6 Sampling.7
7 Test specimens.8
8 Procedure .9
9 Validity of test .12
10 Expression of results .12
11 Test report .12
Annex A (informative) Example of a test report .14
Annex B (informative) Identification of sex of test insects (Anobium punctatum) .16
Annex C (informative) Culturing technique for Anobium punctatum .17
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory .20
Bibliography .21
Foreword
This document (EN 49-2:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of wood
and wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by September 2005, and conflicting national standards shall be
withdrawn at the latest by September 2005.
This document supersedes EN 49-2:1992.
This document consists of two parts, Part 1 is required to enable effectiveness assessments of wood
preservatives which are intended to be applied by surface treatment and Part 2 those which are intended to
be applied by impregnation.
Significant technical differences between this document and EN 49-2:1992 are as follows:
a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests;
b) acknowledgement of the terms given in EN 1001-1;
c) introduction of an informative Annex to take account of consideration for minimisation of environmental
and health hazards caused by the use of this biological test.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.
Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the
concentration at which the product prevents the development of infestation from egg laying.
The method simulates conditions which can occur in practice on timber which has been treated some time
previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In making this
assessment the methods by which the preservative may be applied should be taken into account. It is further
recommended that results from this test should be supplemented by those from other appropriate tests, and
above all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other products,
treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate
rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see
also Annex D for environmental, health and safety precautions).
1 Scope
This document specifies a method for the determination of the protective effectiveness or the toxic values of a
wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has
been treated previously by full impregnation. This method is applicable to:
water-insoluble chemicals which are being studied as active insecticides;
organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;,
organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates, and
water-soluble materials, for example salts.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
2 Normative reference
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the
total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of woos preservative to be tested)
4 Principle
Depending on the test being carried out either
On a set of test specimens of a susceptible wood species that is impregnated with a solution of the
preservative, or
if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that
are impregnated with a series of solutions in which the concentration of preservative is ranged in a given
progression.
The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs laid,
the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in untreated
control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or
by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test
specimens.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.
Adults to be used in the test shall be collected daily from naturally infested wood or laboratory culture (see
Annex C).
Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and
then checked to ensure that they are undamaged, active, and free from any infestation by mites. Determine
the sex (see Annex B) of the collected and checked adults and place the males and females in separate
containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the end sections of test specimens.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and
insoluble in the product under test.
NOTE Sodium carboxymethyl cellulose, food grade, has been found to be suitable.
5.2.3 Xylene, technical grade, mixed isomers.
5.2.4 Water, complying with grade 3 of EN ISO 3696.
5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a
residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.
5.2.7 Fine cloth, of cotton or linen, with a mesh aperture of 0,3 mm to 0,6 mm.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity (80 ± 5) %.
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and relative humidity (65 ± 5) %.
NOTE The conditioning of test specimens can be carried out in the laboratory work area (see 5.3.4) provided that this
has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Treatment vessel(s), of a material that does not react with the preservative under test, for example of
glass for organic products and of polyethylene for salts containing fluorine.
5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their vapours.
5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).
5.3.6 Drying vessel(s), capab
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