EN 15851:2010
(Main)Foodstuffs - Determination of aflatoxin B1 in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection
Foodstuffs - Determination of aflatoxin B1 in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection
This European Standard specifies a method for the determination of aflatoxin B1 in baby food by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 0,07 µg/kg to 0,18 µg/kg.
For further information on the validation, see Clause 9 and Annex B.
Lebensmittel - Bestimmung von Aflatoxin B1 in Säuglings- und Kleinkindernahrung auf Getreidebasis - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule und Fluoreszenzdetektion
Diese Europäische Norm legt ein Verfahren zur Bestimmung von Aflatoxin B1 in Säuglingsnahrung durch
Hochleistungsflüssigchromatographie (HPLC) mit Reinigung an einer Immunoaffinitätssäule und Fluoreszenzdetektion
fest. Dieses Verfahren wurde in einem Ringversuch durch die Untersuchung von natürlich kontaminierten
Proben und aufgestockten Proben mit Gehalten von 0,07 µg/kg bis 0,18 µg/kg validiert.
Weitere Informationen zur Validierung befinden sich in Abschnitt 9 und Anhang B.
Produits alimentaires - Dosage de l'aflatoxine B1 dans les aliments à base de céréales pour nourrissons et enfants en bas âge - Méthode de chromatographie liquide à haute performance avec purification sur colonne d'immuno-affinité et détection par fluorescence
La présente Norme européenne spécifie une méthode pour déterminer la teneur en aflatoxine B1 dans les
aliments pour bébés par chromatographie liquide à haute performance (CLHP) avec purification sur colonne
d’immuno-affinité et détection par fluorescence. Cette méthode a été validée lors d’une étude interlaboratoires
par l’analyse d’échantillons naturellement contaminés et d’échantillons dopés, à des concentrations allant de
0,07 μg/kg à 0,18 μg/kg.
Pour plus d’informations sur la validation, voir l’Article 9 et l’Annexe B.
Živila - Določevanje aflatoksina B1 v žitnih kašicah za dojenčke in majhne otroke - Metoda HPLC z imunoafinitetnim kolonskim čiščenjem in fluorescenčno detekcijo
Ta evropski standard določa metodo za določevanje aflatoksina B1 v hrani za dojenčke z tekočinsko kromatografijo visoke ločljivosti (HPLC) z imunoafinitetnim čiščenjem in fluorescenčno detekcijo. Ta metoda je bila potrjena v medlaboratorijski študiji preko analiz naravno kontaminiranih vzorcev in vzorcev z internimi dodatki v razponu od 0,07 μg/kg do 0,18 μg/kg. Za nadaljnje informacije o potrjevanju, glej Klavzulo 9 in Dodatek B.
General Information
Overview
EN 15851:2010 (CEN) defines a validated analytical method for the determination of aflatoxin B1 in cereal‑based foods for infants and young children (baby food). The standard specifies sample extraction, HPLC analysis with immunoaffinity column (IAC) cleanup and fluorescence detection, including post‑column derivatization options. The method was validated in an interlaboratory study on naturally contaminated and spiked samples (range 0.07 µg/kg to 0.18 µg/kg); see Clause 9 and Annex B for validation data.
Key topics and technical requirements
- Scope and purpose: Quantitative determination of aflatoxin B1 in infant cereal‑based products using RP‑HPLC after IAC cleanup.
- Extraction: Test portion (example: 50 g) extracted with a methanol:water solvent; sodium chloride used as an additive for extraction efficiency.
- Immunoaffinity cleanup: IACs containing antibodies specific to aflatoxin B1; column capacity not less than 100 ng and recovery ≥ 80 % for a 5 ng spike.
- HPLC and detection:
- Reverse‑phase HPLC (C18/ODS) with a guard column to ensure baseline resolution of AFB1.
- Fluorescence detection following post‑column derivatization (PCD) by either pyridinium hydrobromide perbromide (PBPB) or electrochemically generated bromine (e.g., KOBRA cell®).
- Detector settings and mobile phases are specified; LOQ and LOD performance criteria are provided (system LOD ≤ 0.05 µg/kg; LOQ ≤ 0.10 µg/kg with RSD ≤ 10 % at LOQ).
- Reagents and apparatus: Lists solvents (acetonitrile, methanol), PBS, PBPB, degassing, pumps, reaction tubing and safety notes (light sensitivity of aflatoxins, hazardous solvents).
- Quality and validation: Precision and interlaboratory validation results are documented (Annex B). Test report requirements are defined.
Applications - who uses this standard
- Food safety laboratories performing regulatory compliance testing for aflatoxin B1 in infant foods.
- Baby food manufacturers for internal quality control and supplier verification.
- Regulatory authorities and inspection bodies assessing product safety and compliance with maximum levels.
- Research laboratories conducting exposure or risk assessments on aflatoxin contamination in cereal‑based infant foods.
Related standards and practical value
- EN 15851:2010 complements other food analysis standards (e.g., general HPLC and laboratory water quality standards such as EN ISO 3696). Its practical value lies in providing a harmonized, validated protocol for sensitive, trace‑level quantification of aflatoxin B1 - critical for protecting infants from mycotoxin exposure and for ensuring consistent, comparable results across European testing laboratories.
Keywords: EN 15851:2010, aflatoxin B1, HPLC, immunoaffinity column, fluorescence detection, baby food testing, post‑column derivatization, PBPB, KOBRA cell, interlaboratory validation.
Frequently Asked Questions
EN 15851:2010 is a standard published by the European Committee for Standardization (CEN). Its full title is "Foodstuffs - Determination of aflatoxin B1 in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection". This standard covers: This European Standard specifies a method for the determination of aflatoxin B1 in baby food by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 0,07 µg/kg to 0,18 µg/kg. For further information on the validation, see Clause 9 and Annex B.
This European Standard specifies a method for the determination of aflatoxin B1 in baby food by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 0,07 µg/kg to 0,18 µg/kg. For further information on the validation, see Clause 9 and Annex B.
EN 15851:2010 is classified under the following ICS (International Classification for Standards) categories: 67.060 - Cereals, pulses and derived products. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 15851:2010 is associated with the following European legislation: Standardization Mandates: M/383. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Aflatoxin B1 in Säuglings- und Kleinkindernahrung auf Getreidebasis - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule und FluoreszenzdetektionProduits alimentaires - Dosage de l'aflatoxine B1 dans les produits pour nourrissons et jeunes enfants à base de céréales - Méthode de chromatographie liquide haute performance avec purification sur colonne d'immunoaffinité et détection par fluorescenceFoodstuffs - Determination of aflatoxin B1 in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection67.230Predpakirana in pripravljena hranaPrepackaged and prepared foods67.060QMLKCereals, pulses and derived productsICS:Ta slovenski standard je istoveten z:EN 15851:2010SIST EN 15851:2010en,fr,de01-september-2010SIST EN 15851:2010SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 15851
April 2010 ICS 67.060 English Version
Foodstuffs - Determination of aflatoxin B1 in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection
Produits alimentaires - Dosage de l'aflatoxine B1 dans les produits pour nourrissons et jeunes enfants à base de céréales - Méthode de chromatographie liquide haute performance avec purification sur colonne d'immunoaffinité et détection par fluorescence
Lebensmittel - Bestimmung von Aflatoxin B1 in Säuglings- und Kleinkindernahrung auf Getreidebasis - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule und Fluoreszenzdetektion This European Standard was approved by CEN on 27 February 2010.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2010 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15851:2010: ESIST EN 15851:2010
Typical chromatogram . 13Annex B (informative)
Precision data . 14Bibliography . 15
37 % in water. 4.11 Hydrochloric acid solution, substance concentration c(HCl) = 0,1 mol/l. Dilute 8,28 ml of hydrochloric acid solution (4.10) to 1 l with water. 4.12 Sodium hydroxide solution, c(NaOH) = 0,1 mol/l. Dissolve 4 g of sodium hydroxide (4.9) in 1 l of water. 4.13 Phosphate buffered saline (PBS) solution, c(NaCl) = 120 mmol/l, c(KCl) = 2,7 mmol/l, c(phosphate buffer) = 10 mmol/l, pH = 7,4. Dissolve 8,0 g of sodium chloride (4.8), 1,2 g of anhydrous disodium hydrogen phosphate or 2,9 g of Na2HPO4·12 H2O (4.2), 0,2 g of potassium dihydrogen phosphate (4.7) and 0,2 g of potassium chloride (4.6) in 900 ml of water. After dissolution, adjust the pH to 7,4 with hydrochloric acid solution (4.11) or sodium hydroxide solution (4.12) as appropriate, then dilute to 1 l with water. Alternatively, a PBS solution with equivalent properties can be prepared from commercially available PBS material. 4.14 Pyridinium hydrobromide perbromide (PBPB), [CAS: 39416-48-3]. 4.15 Acetonitrile. WARNING — Acetonitrile is hazardous and samples shall be blended using an explosion proof blender which is housed within a fume cupboard. After blending, samples shall be filtered inside a fume cupboard. 4.16 Methanol, HPLC grade. 4.17 Methanol,
technical grade. 4.18 Toluene. 4.19 Extraction solvent. Mix eight parts per volume of methanol (4.17) with two parts per volume of water. 4.20 Nitric acid, c(HNO3) = 4 mol/l. 4.21 HPLC mobile phase A, for use with PBPB. Mix six parts per volume of water with two parts per volume of acetonitrile (4.15) and three parts per volume of methanol (4.16). Degas mobile phase A with for example helium (4.2). 4.22 HPLC mobile phase B, for use with electrochemically generated bromine. Mix six parts per volume of water with two parts per volume of acetonitrile (4.15) and three parts per volume of methanol (4.16). Add 120 mg of potassium bromide (4.5) and 350 µl of nitric acid (4.20) per litre of mobile phase. Degas mobile phase B with for example helium (4.2). 4.23 Post-column reagent. Dissolve 50 mg of PBPB (4.14) in 1 l of water. To be used with mobile phase solvent A (4.21). The solution may be used up to four days if stored in a dark place at room temperature. SIST EN 15851:2010
aflatoxin B1 are applied as a standard solution in a mixture of ten parts per volume of methanol and 90 parts per volume of water. 4.26 Aflatoxin B1, in crystal form or as a film in ampoules or in form of commercially available aflatoxin B1 solution. WARNING — Aflatoxins are subject to light degradation. Protect the laboratory, where the analyses are done, adequately from daylight. This can be achieved effectively by using ultraviolet (UV) absorbing foil on the windows in combination with subdued light (no direct sunlight) or curtains or blinds in combination with artificial light (fluorescent tubes are acceptable). Protect aflatoxin containing solutions from light as much as possible (keep in the dark, use aluminium foil or amber-coloured glassware). 4.27 Aflatoxin B1 stock solution, c ≈ 10 µg/ml. Prepare a solution of aflatoxin B1 in the mixture of toluene and acetonitrile (4.24) to give a solution with a mass concentration of approximately 10 µg/ml. To determine the exact mass concentration, record the absorption curve between 330 nm and 370 nm in 1 cm quartz cells in a spectrometer (5.14) with the mixture of toluene and acetonitrile (4.24) as reference. Identify the wavelength for maximum absorption (between 330 nm and 370 nm). Calculate the mass concentration of aflatoxin B1, afl, in µg/ml, using Equation (1):
bMA×××=ερ100maxafl (1) where
Amax is the absorption determined at the maximum of the absorption curve (between 330 nm and 370 nm);
M is the molar mass, in g/mol, of aflatoxin B1 (M = 312 g/mol);
0 is the molar absorption coefficient, in square metres per mole, of aflatoxin B1 in the mixture of toluene and acetonitrile (4.24) (1 930 m2/mol, see [5]);
b is the optical path length, in centimetres, of the quartz cell. Store this solution in a freezer at approximately - 18 °C. Allow to reach room temperature before opening. A solution stored in this way is usually stable for 12 months. Confirm the concentration of the solution if it is older than 12 months. 4.28 Aflatoxin B1 standard solution,
= 5,00 ng/ml. Pipette a volume of aflatoxin B1 stock solution (4.27) containing exactly 1,00 µg aflatoxin B1 into a 200 ml calibrated volumetric flask and dilute to the mark with the mixture of toluene and acetonitrile (4.24). This solution contains 5,00 ng/ml aflatoxin B1. SIST EN 15851:2010
= 2 µg/ml. Pipette a volume of aflatoxin B1 stock solution (4.27) containing exactly 20 µg aflatoxin B1 into a 10 ml calibrated volumetric flask.
Evaporate the mixture of toluene and acetonitrile solution just to dryness under a stream of nitrogen at room temperature. Make up to volume with methanol (4.16) and shake well. The concentration of this spiking solution is 2 µg/ml for aflatoxin B1. Wrap the flask tightly in aluminium foil and store it at less than 4 °C. Before use, do not remove the aluminium foil until the contents have reached room temperature to avoid incorporation of water by condensation. A solution stored in this way is stable for at least three months. 5 Apparatus WARNING — All glassware coming into contact with aqueous solutions of aflatoxins shall be washed with acid solution before use. Many laboratory washing machines do this as part of the washing programme. Otherwise soak laboratory glassware coming into contact with aqueous solutions of aflatoxins in sulfuric acid (c = 2 mol/l) for several hours (e.g. 15 h overnight), then rinse well (e.g. at least three times) with water to remove all traces of acid. Check the absence of acid with pH paper.
This treatment is necessary, because the use of non-acid washed glassware can cause losses of aflatoxins. In practice, the treatment is necessary for round bottomed flasks, volumetric flasks, measuring cylinders, vials or tubes used for calibration solutions and final extracts (particularly autosampler vials), and Pasteur pipettes, if these are used to transfer calibration solutions or extracts. Usual laboratory glassware and equipment and, in particular, the following. 5.1 Analytical balance, capable of weighing to 0,000 1 g. 5.2 Laboratory balance, capable of weighing to 0,01 g. 5.3 Adjustable vertical or horizontal shaker. 5.4 Filter paper, e.g. 24 cm diameter, prefolded. 5.5 Conical flask, with screw top or glass stopper of 500 ml capacity. 5.6 Glass microfibre filter, retention size 1,6 µm or smaller. 5.7 Reservoir, of 75 ml capacity with luer tip connector and attachments for immunoaffinity column (IAC). 5.8 Hand pump, 20 ml syringe with luer lock or rubber stopper for IAC. 5.9 Volumetric flasks, of 5 ml, 10 ml, 20 ml, 150 ml, and 200 ml capacity with an accuracy of at least 0,5 %. 5.10 Disposable syringe filter unit, with pore size of 0,45 µm. Prior to usage, verify that no aflatoxin losses occur during filtrat
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SIST EN 15851:2010は、乳幼児向けの食品中のアフラトキシンB1を高性能液体クロマトグラフィー（HPLC）を使用して決定する方法を示した重要な標準です。この標準は、免疫親和性カラムクリーンアップと蛍光検出を組み合わせることで、食品安全性の重要な側面をカバーしています。 この標準の主な強みは、徐々に厳しくなる食材安全基準に対応できることです。特に、乳幼児という特定の消費者層を対象としているため、安全性が最優先されます。EN 15851:2010では、自然汚染およびスパイクされた試料の解析を通じて、0.07 µg/kgから0.18 µg/kgの範囲でのアフラトキシンB1の定量が検証されており、信頼性の高い結果を得るための確固たる基盤が確立されています。 さらに、この標準は、各国の規制機関や食品メーカーとの調和を図るための共通の指針を提供しており、EU全域での食品の質と安全性を保証する役割を果たします。特に、クリーンアッププロセスが免疫親和性カラムを使用しているため、より高い選択性と感度を実現し、低濃度のアフラトキシンの検出が可能になります。 このように、SIST EN 15851:2010は、乳幼児向け食品におけるアフラトキシンB1の管理において非常に重要な役割を果たしており、食品業界における安全性確保に貢献しています。
The standard EN 15851:2010 addresses the critical issue of food safety by providing a robust method for determining aflatoxin B1 levels in cereal-based foods intended for infants and young children. This European Standard specifically outlines a high-performance liquid chromatography (HPLC) protocol, complemented by immunoaffinity column cleanup and fluorescence detection, which is essential for ensuring accurate measurement of this harmful toxin. One of the significant strengths of this standard is its validation through an interlaboratory study, which enhances the credibility of the method. By encompassing a range of concentrations from 0.07 µg/kg to 0.18 µg/kg, the standard effectively covers scenarios of both naturally contaminated and artificially spiked samples, thus providing a comprehensive framework for laboratories to assess aflatoxin B1 in various applications. The emphasis on the HPLC technique showcases the standard's relevance to contemporary analytical practices, as this method is known for its sensitivity and precision in detecting low levels of contaminants. Moreover, the inclusion of immunoaffinity cleanup indicates a sophisticated approach to sample preparation that minimizes interferences, thereby improving the reliability of the results. Overall, EN 15851:2010 is a crucial standard for the food industry, especially concerning infant nutrition, where safety is paramount. It sets a benchmark for laboratories and quality control processes, ensuring that products are thoroughly tested for aflatoxin B1, thus protecting vulnerable populations and reinforcing public health initiatives.
La norme EN 15851:2010 constitue un outil essentiel pour assurer la sécurité alimentaire, en particulier pour les aliments destinés aux nourrissons et jeunes enfants. Cette norme fournit une méthode spécifiée pour la détermination de l'aflatoxine B1 dans les préparations pour bébés, utilisant la chromatographie liquide à haute performance (HPLC) avec nettoyage par colonne d'immunoaffinité et détection par fluorescence. L'étendue de cette norme est cruciale, car elle cible des produits alimentaires sensibles, garantissant ainsi que les aliments pour nourrissons ne dépassent pas les niveaux acceptables de contamination par les aflatoxines. La méthode a été validée par une étude interlaboratoire, apportant une assurance supplémentaire concernant sa fiabilité dans l'analyse d'échantillons contaminés naturellement et de ceux artificiellement enrichis, allant de 0,07 µg/kg à 0,18 µg/kg. Parmi les forces de cette norme, sa capacité à utiliser une méthode HPLC sophistiquée permet une détection précise et sensible des contaminants, ce qui est particulièrement pertinent dans le contexte actuel où la sécurité alimentaire est une préoccupation majeure. De plus, la mise en œuvre de la purification par immunoaffinité contribue à réduire les interférences et à améliorer la spécificité des résultats, renforçant ainsi la confiance des consommateurs et des producteurs. La norme EN 15851:2010 est d'une grande pertinence, non seulement pour les laboratoires d'analyse, mais aussi pour les fabricants d'aliments pour bébés qui doivent se conformer aux réglementations strictes concernant la sécurité alimentaire. Elle soutient les efforts pour protéger la santé des jeunes enfants, tout en fournissant une base scientifique solide pour le contrôle qualité dans l'industrie alimentaire.
SIST EN 15851:2010 표준은 유아 및 어린이용 시리얼 기반 식품에서 아플라톡신 B1을 검출하기 위한 고성능 액체 크로마토그래피(HPLC) 방법을 규정하고 있습니다. 이 표준은 아플라톡신 B1의 안전성을 보장하는 데 필수적이며, 유아 음식의 품질 관리에 기여합니다. 표준의 주요 강점은 면역 친화성 정화 및 형광 검출을 통한 정확한 아플라톡신 B1 측정 방법을 제공한다는 점입니다. 이는 자연 오염된 샘플과 인위적으로 오염된 샘플 모두에서 유효성을 입증받아 신뢰성 높은 데이터를 제공합니다. 특히 0.07 µg/kg에서 0.18 µg/kg 범위의 분석을 통해 다양한 상황에서의 적용 가능성을 보여주며, 이로 인해 유아 음식의 안전성을 철저히 검증할 수 있습니다. 또한, 표준은 제9조와 부록 B에서 자세한 검증 정보를 제공하여, 검증 과정에 대한 이해를 높이고, 실험실 간 비교 연구를 촉진합니다. 이는 각 실험실이 동일한 기준에서 테스트를 수행할 수 있도록 지원하며, 결과의 일관성을 보장하는 데 중요한 역할을 합니다. SIST EN 15851:2010은 유아 식품의 아플라톡신 B1 측정 방법에 대한 명확한 지침을 제공함으로써, 소비자 보호와 식품 안전성 증진에 기여하는 매우 relevant한 표준입니다. 따라서 이 표준의 적용은 유아에게 안전한 식품을 제공하는 데 있어 필수적인 요소로 여겨집니다.
Die Norm EN 15851:2010 bietet eine umfassende und präzise Methode zur Bestimmung von Aflatoxin B1 in Babynahrung mittels Hochleistungsflüssigkeitschromatographie (HPLC) mit Immunaffinitätsreinigung und Fluoreszenzdetektion. Der Anwendungsbereich dieser Europäischen Norm ist besonders relevant, da er eine essentielle Sicherheitsmaßnahme für die Ernährung von Säuglingen und Kleinkindern darstellt. Die Stärken dieser Norm liegen in der Validierung durch eine umfassende interlaboratorielle Studie, die sowohl natürlich kontaminierte als auch gezielt angereicherte Proben behandelt hat. Die Analyse deckt einen Konzentrationsbereich von 0,07 µg/kg bis 0,18 µg/kg ab, was eine präzise Kontrolle der Aflatoxin B1-Werte in Babylebensmitteln ermöglicht. Diese Validierung sichert die Zuverlässigkeit und Genauigkeit der Methode, was für die Gesundheits- und Sicherheitsstandards im Bereich der Lebensmittel entscheidend ist. Die Relevanz der EN 15851:2010 kann nicht hoch genug gewürdigt werden, da Aflatoxine bekanntermaßen krebserregend sind und besonders für die empfindlichen Zielgruppen wie Säuglinge und Kleinkinder eine ernsthafte Gesundheitsgefahr darstellen. Durch die Bereitstellung eines klaren und standardisierten Verfahrens zur Überwachung dieser gefährlichen Kontaminanten leistet die Norm einen wesentlichen Beitrag zum öffentlichen Gesundheitswesen und zur Sicherstellung der Qualität von Babynahrung. Zusammenfassend zielt die Norm darauf ab, einen effektiven und verlässlichen Ansatz zur Bestimmung von Aflatoxin B1 bereitzustellen, was sie zu einem unverzichtbaren Instrument für Forschungseinrichtungen, Lebensmitteltester und Hersteller von Babynahrung macht.
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