CEN/TR 16193:2013

Sludge, treated biowaste and soil - Detection and enumeration of Escherichia coli

This Technical Report specifies three methods for the detection and enumeration of Escherichia coli in sludge, treated biowaste and soil:
-   Method A - Membrane filtration method for quantification (see Clause 6);
-   Method B - Miniaturised method (Most Probable Number, MPN) by inoculation in liquid medium (see Clause 7);
-   Method C - Macromethod (Most Probable Number) in liquid medium (see Clause 8).

Schlamm, behandelter Bioabfall und Boden - Nachweis und Zählung von Escherichia coli

Dieser Fachbericht beschreibt drei Verfahren zur Bestimmung und Auszählung von Escherichia coli in Schlamm, behandeltem Bioabfall und Boden:
   Verfahren A - Membranfiltrationsverfahren zur quantitativen Bestimmung (siehe Abschnitt 6);
   Verfahren B - Miniaturisiertes Verfahren durch Animpfen in Flüssigmedium (MPN-Verfahren) (siehe Abschnitt 7);
   Verfahren C - Makroverfahren (MPN) in Flüssigmedium (siehe Abschnitt 8).

Boue, biodéchet traité et sol - Recherche et dénombrement des Escherichia coli

Le présent Rapport technique spécifie trois méthodes de recherche et de dénombrement des Escherichia coli
dans les boues, les biodéchets traités et les sols :
 Méthode A - Méthode par filtration sur membrane pour le dénombrement (voir Article 6) ;
 Méthode B - Méthode miniaturisée (nombre le plus probable, NPP) pour ensemencent en milieu liquide
(voir Article 7) ;
 Méthode C - Macrométhode (nombre le plus probable) en milieu liquide (voir Article 8).

Blato, obdelani biološki odpadki in tla - Ugotavljanje prisotnosti in števila Escherichia coli

To tehnično poročilo določa tri metode za ugotavljanje prisotnosti in števila Escherichia coli v blatu, obdelanih bioloških odpadkih in tleh:
– metoda A – metoda filtriranja z membrano za kvantifikacijo (glej točko 6);
– metoda B – miniaturizirana metoda (najbolj verjetno število (MPN)) z inokulacijo v tekoči medij (glej točko 7);
– metoda C – makrometoda (najbolj verjetno število) v tekočem mediju (glej točko 8).

General Information

• Status: Published
• Publication Date: 21-May-2013
• ICS: 13.030.01 - Wastes in general
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444/WG 4 - Biological characterization
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 09-Jun-2020
• Directive: 86/278/EEC - Protection of the environment, and in particular of the soil, when sewage sludge is used in agriculture
• Mandate: M/330 - Mandate given to CEN for the development of horizontal standards in the fields of sludge, biowaste and soil.
• Ref Project: SIST-TP CEN/TR 16193:2013 - Sludge, treated biowaste and soil - Detection and enumeration of Escherichia coli

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TP CEN/TR 16193:2013
01-julij-2013
Blato, obdelani biološki odpadki in tla - Ugotavljanje prisotnosti in števila
Escherichia coli
Sludge, treated biowaste and soil - Detection and enumeration of Escherichia coli
Schlamm, behandelter Bioabfall und Boden - Nachweis und Zählung von Escherichia coli
Boue, biodéchet traité et sol - Recherche et dénombrement des Escherichia coli
Ta slovenski standard je istoveten z: CEN/TR 16193:2013
ICS:
13.030.20 7HNRþLRGSDGNL%ODWR Liquid wastes. Sludge
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST-TP CEN/TR 16193:2013 en,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TP CEN/TR 16193:2013

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SIST-TP CEN/TR 16193:2013


TECHNICAL REPORT
CEN/TR 16193

RAPPORT TECHNIQUE

TECHNISCHER BERICHT
May 2013
ICS 13.030.01
English Version
Sludge, treated biowaste and soil - Detection and enumeration
of Escherichia coli
Boue, biodéchet traité et sol - Recherche et dénombrement Schlamm, behandelter Bioabfall und Boden - Nachweis und
des Escherichia coli Zählung von Escherichia coli


This Technical Report was approved by CEN on 1 March 2011. It has been drawn up by the Technical Committee CEN/TC 400.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 16193:2013: E
worldwide for CEN national Members.

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SIST-TP CEN/TR 16193:2013
CEN/TR 16193:2013 (E)
Contents Page
Foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Abbreviations . 7
5 Quality assurance . 7
6 Method A — Membrane filtration method for quantification . 7
6.1 Scope . 7
6.2 Principle. 8
6.3 Reagents, diluents and culture media . 8
6.4 Apparatus . 9
6.5 Sampling . 10
6.5.1 General . 10
6.5.2 Storage . 11
6.5.3 Handling . 11
6.6 Procedure . 11
6.6.1 Sample preparation . 11
6.6.2 Sample dilution
...