EN ISO 23275-2:2008

Animal and vegetable fats and oils - Cocoa butter equivalents in cocoa butter and plain chocolate - Part 2: Quantification of cocoa butter equivalents (ISO 23275-2:2006)

ISO 23275-2:2006 specifies a procedure for the quantification of cocoa butter equivalents (CBEs) in cocoa butter and plain chocolate by high-resolution capillary gas chromatography (HR-GC) of triacylglycerols, and subsequent data evaluation by partial least squares regression analysis.

Tierische und pflanzliche Fette und Öle - Kakaobutter-Äquivalente in Kakaobutter und Zartbitterschokolade - Teil 2: Quantifizierung von Kakaobutter-Äquivalenten (ISO 23275-2:2006)

Dieser Teil von ISO 23275 legt ein Verfahren zur Mengenbestimmung von Kakaobutter-Äquivalenten (CBE) in
Kakaobutter und Zartbitterschokolade durch hochauflösende Kapillar-Gaschromatographie (HR-GC) von
Triacylglycerinen und anschließende Datenauswertung durch partielle Regressionsanalyse nach der Methode
der kleinsten Quadrate fest.
ANMERKUNG Das Vorliegen von CBE in Kakaobutter und Zartbitterschokolade kann noch bis zu einem Gehalt von
0,6 % (der angenommene Fettgehalt der Schokolade beträgt 30 %) durch Anwendung des in ISO 23275-1 erläuterten
Verfahrens bestimmt werden. Unterschiede bei der Durchführung der beiden Verfahren bestehen in der Anzahl der
einzelnen Triacylglycerine, die für die Datenauswertung verwendet werden, und im Prinzip der mathematischen
Auswertung der Daten. Das Vorliegen von CBE wird durch lineare Regressionsanalyse nachgewiesen, die auf die
relativen Anteile der drei hauptsächlichen Triacylglcerin-Fraktionen des analysierten Fettes angewendet wird. Die Menge
des CBE-Zusatzes wird durch partielle Regressionsanalyse nach der Methode der kleinsten Quadrate abgeschätzt, die
auf die relativen Anteile der fünf hauptsächlichen Triacylglcerine angewendet wird.

Corps gras d'origines animale et végétale - Équivalents au beurre de cacao dans le beurre de cacao et dans le chocolat de ménage - Partie 2: Quantification des équivalents au beurre de cacao (ISO 23275-2:2006)

L'ISO 23275-2:2006 spécifie un mode opératoire permettant la quantification des équivalents au beurre de cacao (EBC) dans le beurre de cacao (BC) et le chocolat de ménage par analyse chromatographique en phase gazeuse/liquide capillaire haute résolution (HR-GC) des triacylglycérols, et l'évaluation ultérieure des données par analyse de régression par la méthode des moindres carrés partiels.

Živalske in rastlinske maščobe in olja - Ekvivalenti kakavovega masla v kakavovem maslu in navadni čokoladi - 2. del: Kvantitativno določevanje ekvivalentov kakavovega masla (ISO 23275-2:2006)

General Information

• Status: Published
• Publication Date: 11-Nov-2008
• Withdrawal Date: 30-May-2009
• ICS: 67.190 - Chocolate
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 12-Nov-2008
• Due Date: 25-Jan-2010
• Completion Date: 12-Nov-2008
• Ref Project: SIST EN ISO 23275-2:2009 - Animal and vegetable fats and oils - Cocoa butter equivalents in cocoa butter and plain chocolate - Part 2: Quantification of cocoa butter equivalents (ISO 23275-2:2006)

Standards Content (Sample)

SLOVENSKI STANDARD
01-april-2009
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD(NYLYDOHQWLNDNDYRYHJDPDVODY
NDNDYRYHPPDVOXLQQDYDGQLþRNRODGLGHO.YDQWLWDWLYQRGRORþHYDQMH
HNYLYDOHQWRYNDNDYRYHJDPDVOD,62
Animal and vegetable fats and oils - Cocoa butter equivalents in cocoa butter and plain
chocolate - Part 2: Quantification of cocoa butter equivalents (ISO 23275-2:2006)
Tierische und pflanzliche Fette und Öle - Kakaobutter-Äquivalente in Kakaobutter und
Zartbitterschokolade - Teil 2: Quantifizierung von Kakaobutter-Äquivalenten (ISO 23275-
2:2006)
Corps gras d'origines animale et végétale - Équivalents au beurre de cacao dans le
beurre de cacao et dans le chocolat de ménage - Partie 2: Quantification des équivalents
au beurre de cacao (ISO 23275-2:2006)
Ta slovenski standard je istoveten z: EN ISO 23275-2:2008
ICS:
67.190 ýRNRODGD Chocolate
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 23275-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2008
ICS 67.200.10; 67.190
English Version
Animal and vegetable fats and oils - Cocoa butter equivalents in
cocoa butter and plain chocolate - Part 2: Quantification of
cocoa butter equivalents (ISO 23275-2:2006)
Corps gras d'origines animale et végétale - Équivalents au Tierische und pflanzliche Fette und Öle - Kakaobutter-
beurre de cacao dans le beurre de cacao et dans le Äquivalente in Kakaobutter und Zartbitterschokolade - Teil
chocolat de ménage - Partie 2: Quantification des 2: Mengenbestimmung von Kakaobutter-Äquivalenten (ISO
équivalents au beurre de cacao (ISO 23275-2:2006) 23275-2:2006)
This European Standard was approved by CEN on 23 October 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23275-2:2008: E
worldwide for CEN national Members.

Contents Page
Foreword.3

Foreword
The text of ISO 23275-2:2006 has been prepared by Technical Committee ISO/TC 34 “Agricultural food
products” of the International Organization for Standardization (ISO) and has been taken over as EN ISO
23275-2:2008 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their
by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by May 2009, and conflicting national standards shall be withdrawn at the
latest by May 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 23275-2:2006 has been approved by CEN as a EN ISO 23275-2:2008 without any
modification.
INTERNATIONAL ISO
STANDARD 23275-2
First edition
2006-11-01
Animal and vegetable fats and oils —
Cocoa butter equivalents in cocoa butter
and plain chocolate —
Part 2:
Quantification of cocoa butter
equivalents
Corps gras d'origines animale et végétale — Équivalents au beurre
de cacao dans le beurre de cacao et dans le chocolat de ménage —
Partie 2: Quantification des équivalents au beurre de cacao

Reference number
ISO 23275-2:2006(E)
©
ISO 2006
ISO 23275-2:2006(E)
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ii © ISO 2006 – All rights reserved

ISO 23275-2:2006(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope .1
2 Terms and definitions .1
3 Principle.1
4 Reagents and materials .2
5 Apparatus .2
6 Sampling.3
7 Preparation of test sample.3
7.1 Preparation of cocoa butter CRM for calibration purposes and system suitability check.3
7.2 Preparation of chocolate sample .3
8 Procedure .3
8.1 Fat extraction .3
8.2 Separation of individual triacylglycerols by HR-GC .4
8.3 Identification.4
9 Calculation.4
9.1 Determination of response factors .4
9.2 Calculation of percentages of triacylglycerols.5
9.3 Calculation of CBE content in cocoa butter .5
9.4 Calculation of CBE content in chocolate .6
10 Procedural requirements .6
10.1 General.6
10.2 System suitability .6
11 Precision.6
11.1 Interlaboratory test .6
11.2 Repeatability.7
11.3 Reproducibility.7
12 Test report .7
Annex A (informative) Results of interlaboratory test.8
Bibliography .13

ISO 23275-2:2006(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 23275-2 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
ISO 23275 consists of the following parts, under the general title Animal and vegetable fats and oils — Cocoa
butter equivalents in cocoa butter and plain chocolate:
⎯ Part 1: Determination of the presence of cocoa butter equivalents
⎯ Part 2: Quantification of cocoa butter equivalents
iv © ISO 2006 – All rights reserved

ISO 23275-2:2006(E)
Introduction
“Cocoa butter equivalents” is the general term for fats used to replace cocoa butter in chocolate. They
resemble the chemical composition and physical properties of cocoa butter very closely, making them
therefore extremely difficult to quantify and even in some cases to detect. In principle, cocoa butter
equivalents must by definition be fats low in lauric acid, rich in symmetrical mono-unsaturated triacylglycerols
of the type 1,3-dipalmitoyl-2-oleoylglycerol, 1-palmitoyl-2-oleoyl-3-stearoylglycerol and 1,3-distearoyl-2-
oleoylglycerol, miscible with cocoa butter, and obtained only by refining and fractionation.
Within the European Union, the following vegetable fats, obtained from the plants listed below, may be used
[1]
singly or in blends, according to Directive 2000/36/EC :
⎯ illipé, Borneo tallow or tengkawang (Shorea spp.),
⎯ palm oil (Elaeis guineensis, Elaeis olifera),
...