EN 1104:2018
(Main)Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents
Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents
This document specifies a method for the determination of transfer of antimicrobial constituents from paper and board materials and articles intended for food contact.
NOTE The need of using this Standard may be specified by the legislation regarding paper and board intended to come into contact with foodstuffs.
Papier und Pappe vorgesehen für den Kontakt mit Lebensmitteln - Bestimmung des Übergangs antimikrobieller Bestandteile
Dieses Dokument legt ein Verfahren zur Bestimmung der Übertragung antimikrobieller Bestandteile von Materialien und Artikeln aus Papier und Pappe fest, die für den Kontakt mit Lebens¬mitteln vorgesehen sind.
ANMERKUNG Die Notwendigkeit der Verwendung dieser Norm kann durch die Vorschriften für Papier und Pappe, die für den Kontakt mit Lebensmitteln vorgesehen sind, festgelegt sein.
Papier et carton destinés à entrer en contact avec les denrées alimentaires - Détermination du transfert des constituants antimicrobiens
Le présent document spécifie une méthode de détermination du transfert des constituants antimicrobiens des papiers, cartons et articles destinés à entrer en contact avec des denrées alimentaires.
NOTE La nécessité d’utiliser la présente norme peut être spécifiée par la législation relative au papier et au carton destinés à entrer en contact avec des denrées alimentaires.
Papir, karton in lepenka v neposrednem stiku z živili - Določanje izločanja antimikrobnih snovi
Ta evropski standard določa metodo za določanje izločanja antimikrobnih snovi iz papirnatih in kartonskih materialov ter izdelkov, namenjenih za stik z živili.
OPOMBA: Potreba po uporabi tega standarda je lahko določena z zakonodajo v zvezi s papirjem in kartonom, namenjenim za neposreden stik z živili.
General Information
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Papier und Pappe vorgesehen für den Kontakt mit Lebensmitteln - Bestimmung des Übergangs antimikrobieller BestandteilePapier et carton destinés à entrer en contact avec les denrées alimentaires - Détermination du transfert des constituants antimicrobiensPaper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents85.060Papir, karton in lepenkaPaper and board67.250Materiali in predmeti v stiku z živiliMaterials and articles in contact with foodstuffsICS:Ta slovenski standard je istoveten z:EN 1104:2018SIST EN 1104:2019en,fr,de01-januar-2019SIST EN 1104:2019SLOVENSKI
STANDARDSIST EN 1104:20051DGRPHãþD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 1104
November
t r s z ICS
x yä t w râ
z wä r x r English Version
Paper and board intended to come into contact with foodstuffs æ Determination of the transfer of antimicrobial constituents Papier et carton destinés à entrer en contact avec les denrées alimentaires æ Détermination du transfert des constituants antimicrobiens
Papier und Pappe vorgesehen für den Kontakt mit Lebensmitteln æ Bestimmung des Übergangs antimikrobieller Bestandteile This European Standard was approved by CEN on
s w July
t r s zä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Serbiaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Rue de la Science 23,
B-1040 Brussels
t r s z CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s s r vã t r s z ESIST EN 1104:2019
Page European foreword . 4 1 Scope . 5 2 Normative references . 5 3 Terms and definitions . 5 4 Principle . 5 5 Apparatus . 6 6 Reagents and media . 6 6.1 General . 6 6.2 Non ionic wetting agent . 6 6.3 Nutrient medium for the preparation of Bacillus subtilis spores . 6 6.3.1 Composition . 6 6.3.2 Preparation . 6 6.4 Nutrient medium of Sabouraud for the preparation of Aspergillus niger spores . 7 6.4.1 Composition . 7 6.4.2 Preparation . 7 6.5 Nutrient medium for the inhibition test with Bacillus subtilis . 7 6.5.1 Composition . 7 6.5.2 Preparation . 7 6.6 Nutrient medium of modified Sabouraud for the inhibition test with Aspergillus niger . 8 6.6.1 Composition . 8 6.6.2 Preparation . 8 6.7 Tryptone salt solution . 8 6.7.1 Composition . 8 6.7.2 Preparation . 8 6.8 Test microorganisms . 8 6.8.1 General . 8 6.8.2 Preparation of working cultures . 8 6.8.3 Preparation of inoculating spore suspension of Bacillus subtilis . 9 6.8.4 Preparation of inoculating spore suspension of Aspergillus niger . 9 6.8.5 Concentrations of spores for inhibition test . 9 6.9 Positive controls . 10 6.9.1 Antibiotic . 10 6.9.2 Antifungal agent . 10 7 Sampling and preparation of test pieces . 10 8 Procedure. 10 8.1 General . 10 8.2 Preparation of plates . 10 8.2.1 General . 10 8.2.2 Test with Bacillus subtilis . 10 8.2.3 Test with Aspergillus niger . 11 8.3 Incubation . 11 9 Evaluation . 11 SIST EN 1104:2019
Guidelines for interpreting results . 13 A.1 General . 13 A.2 Negative and positive controls . 13 A.2.1 Negative controls . 13 A.2.2 Positive controls . 14 A.3 Paper and board samples . 15 A.3.1 General . 15 A.3.2 Case of Bacillus subtilis . 15 A.3.3 Case of Aspergillus niger . 19
IEC Electropedia: available at http://www.electropedia.org/
ISO Online browsing platform: available at http://www.iso.org/obp 3.1 inhibition zone obvious area in which growth is absent and which forms around test pieces placed on a nutrient medium inoculated with a preselected test microorganism, due to the release of water-soluble antimicrobial constituents; proof of the presence of an inhibition zone is provided by the absence of test micro-organism growth (translucent zone) in a minimum of 2 mm width zone at the edges of the test pieces 4 Principle A prepared nutrient medium is mixed with an appropriate inoculum and poured into Petri dishes. The test pieces are placed on the nutrient medium before its complete solidification and then incubated. When incubation is terminated, the existence of an inhibition zone is an indicator of the release of antimicrobial constit
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