Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil materials - Part 4: Influence of pH on leaching with initial acid/base addition (ISO/TS 21268-4:2007)

ISO/TS 21268-4:2007 specifies a test method to obtain information on the short- and long-term leaching behaviour and characteristic properties of materials. It applies to the determination of the influence of pH on the leachability of inorganic and organic constituents from soil and soil material, and the ecotoxicological effects of eluates with respect to microorganisms, fauna and flora. The test is not suitable for constituents that are volatile under ambient conditions.
The test procedure specified in ISO/TS 21268-4:2007 produces eluates that are subsequently characterized by physical, chemical and ecotoxicological standard methods.

Bodenbeschaffenheit - Eluierungsverfahren für die anschließende chemische und ökotoxikologische Untersuchung von Boden und von Bodenmaterialien - Teil 4: Einfluss des pH–Wertes unter vorheriger Säure/Base-Zugabe (ISO/TS 21268-4:2007)

Dieser Teil von ISO/TS 21268 legt ein Prüfverfahren fest, um Angaben über das kurzfristige und langfristige
Auslaugungsverhalten und zu den charakteristischen Eigenschaften von Materialien zu erhalten.
Er wird angewendet, um den Einfluss des pH-Wertes auf die Auslaugbarkeit anorganischer und organischer
Bestandteile aus Böden und Bodenmaterialien sowie die ökotoxikologischen Auswirkungen von Eluaten auf
Mikroorganismen, Fauna und Flora zu untersuchen. Die Prüfung ist nicht geeignet für Bestandteile, die unter
Umgebungsbedingungen flüchtig sind. Der Gleichgewichtszustand, wie er in diesem Teil von ISO/TS 21268
definiert ist, wird durch Zugabe von vorher bestimmten Mengen an Säure oder Base hergestellt, um die
gewünschten endgültigen pH-Werte zu erreichen.
Durch das in diesem Teil von ISO/TS 21268 festgelegte Prüfverfahren werden Eluate hergestellt, die
anschließend physikalisch, chemisch und ökotoxikologisch nach Standardverfahren charakterisiert werden.
Für die Durchführung ökotoxikologischer Untersuchungen liegt der betreffende pH-Wert-Bereich (siehe 9.2)
üblicherweise zwischen 5 und 9.
ANMERKUNG 1 Zu den flüchtigen organischen Bestandteilen gehören niedermolekulare Komponenten in
Mischungen, wie z. B. Mineralöl.
ANMERKUNG 2 Es ist nicht immer möglich, die Prüfbedingungen gleichzeitig für anorganische und organische
Bestandteile zu optimieren, und die optimalen Prüfbedingungen können sich auch für unterschiedliche Gruppen von
organischen Bestandteilen unterscheiden. Die Prüfanforderungen für organische Bestandteile sind im Allgemeinen
strenger als für anorganische Bestandteile. Die Prüfbedingungen, die für die Messung der Freisetzung von organischen
Bestandteilen geeignet sind, sind im Allgemeinen auch auf anorganische Bestandteile anwendbar.
ANMERKUNG 3 Für ökotoxikologische Untersuchungen sind Eluate erforderlich, die die Freisetzung sowohl von
anorganischen als auch von organischen Schadstoffen widerspiegeln.

Qualité du sol - Modes opératoires de lixiviation en vue d'essais chimiques et écotoxicologiques ultérieurs des sols et matériaux du sol - Partie 4: Essai de dépendance au pH avec ajout initial d'acide/base (ISO/TS 21268-4:2007)

L'ISO/TS 21268-4:2007 spécifie une méthode d'essai pour obtenir des informations sur le comportement à la lixiviation à court terme et à long terme ainsi que sur les propriétés caractéristiques des matériaux. Elle s'applique à la détermination de l'influence du pH sur la lixiviabilité des constituants organiques et inorganiques du sol et des matériaux du sol, ainsi que sur les effets écotoxicologiques des éluats sur les micro-organismes, la faune et la flore. L'essai n'est pas adapté à des espèces qui sont volatiles dans des conditions ambiantes.
Le mode opératoire d'essai spécifié dans l'ISO/TS 21268-4:2007 permet d'obtenir des éluats qui sont ensuite caractérisés par des méthodes physiques, chimiques et écotoxicologiques normalisées.

Kakovost tal - Postopki izluževanja za nadaljnje kemijsko in ekotoksikološko preskušanje tal in talnih (zemeljskih) materialov - 4. del: Vpliv pH na izluževanje z začetnim dodatkom kisline ali baze (ISO/TS 21268-4:2007)

Ta del ISO/TS 21268 opredeljuje preskusno metodo za pridobivanje informacij o kratkoročnem in dolgoročnem odzivu na izluževanje ter značilnih lastnosti materialov. Velja za določitev vpliva pH na sposobnost izluževanja neorganskih in organskih sestavin iz tal in talnih (zemeljskih) materialov ter ekotoksiološke učinke izlužkov v zvezi z mikroorganizmi, favno in floro. Preskus ni primeren za sestavine, ki so hlapne pod okoljskimi pogoji. Pogoj za ravnovesje, opredeljen v tem delu ISO/TS 21268, je vzpostavljen z dodanimi vnaprej določenimi količinami kisline ali baze za doseganje želenih končnih pH vrednosti. Preskusni postopek, opredeljen v tem delu ISO/TS 21268, prinaša izlužke, ki so nadalje določeni s fizičnimi, kemičnimi in z ekotoksiološkimi standardnimi metodami. Za namene ekotoksioloških preskusov bo običajno ustrezni razpon pH (glej točko 9.2) med pH 5 do 9. Ta preskus se ne more uporabljati sam za določevanje celotnega odziva tal na izluževanje. Za tak razširjen cilj je potrebnih več preskusov izluževanja. Ta del ISO/TS 21268 ne naslavlja vprašanj glede zdravja in varnosti. Kot je navedeno v Klavzuli 5, zgolj določa lastnosti izluženega.

General Information

Status
Withdrawn
Publication Date
03-Nov-2009
Withdrawal Date
03-Dec-2019
Current Stage
9960 - Withdrawal effective - Withdrawal
Completion Date
04-Dec-2019

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SLOVENSKI STANDARD
SIST-TS CEN ISO/TS 21268-4:2010
01-februar-2010
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SUHVNXãDQMHWDOLQWDOQLK ]HPHOMVNLK PDWHULDORYGHO9SOLYS+QDL]OXåHYDQMH]

]DþHWQLPGRGDWNRPNLVOLQHDOLED]H ,6276

Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing

of soil and soil materials - Part 4: Influence of pH on leaching with initial acid/base

addition (ISO/TS 21268-4:2007)
Bodenbeschaffenheit - Eluierungsverfahren für die anschließende chemische und

ökotoxikologische Untersuchung von Boden und von Bodenmaterialien - Teil 4: Einfluss

des pH–Wertes unter vorheriger Säure/Base-Zugabe (ISO/TS 21268-4:2007)
Qualité du sol - Modes opératoires de lixiviation en vue d'essais chimiques et
écotoxicologiques ultérieurs des sols et matériaux du sol - Partie 4: Essai de
dépendance au pH avec ajout initial d'acide/base (ISO/TS 21268-4:2007)
Ta slovenski standard je istoveten z: CEN ISO/TS 21268-4:2009
ICS:
13.080.05 Preiskava tal na splošno Examination of soils in
general
SIST-TS CEN ISO/TS 21268-4:2010 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN ISO/TS 21268-4:2010
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SIST-TS CEN ISO/TS 21268-4:2010
TECHNICAL SPECIFICATION
CEN ISO/TS 21268-4
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
November 2009
ICS 13.080.05
English Version
Soil quality - Leaching procedures for subsequent chemical and
ecotoxicological testing of soil and soil materials - Part 4:
Influence of pH on leaching with initial acid/base addition
(ISO/TS 21268-4:2007)

Qualité du sol - Modes opératoires de lixiviation en vue Bodenbeschaffenheit - Eluierungsverfahren für die

d'essais chimiques et écotoxicologiques ultérieurs des sols
anschließende chemische und ökotoxikologische

et matériaux du sol - Partie 4: Essai de dépendance au pH Untersuchung von Boden und von Bodenmaterialien - Teil

avec ajout initial d'acide/base (ISO/TS 21268-4:2007) 4: Einfluss des pH-Wertes unter vorheriger Säure/Base-

Zugabe (ISO/TS 21268-4:2007)

This Technical Specification (CEN/TS) was approved by CEN on 8 September 2009 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their

comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available

promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)

until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,

France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,

Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN ISO/TS 21268-4:2009: E

worldwide for CEN national Members.
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SIST-TS CEN ISO/TS 21268-4:2010
CEN ISO/TS 21268-4:2009 (E)
Contents Page

Foreword ..............................................................................................................................................................3

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SIST-TS CEN ISO/TS 21268-4:2010
CEN ISO/TS 21268-4:2009 (E)
Foreword

The text of ISO/TS 21268-4:2007 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the

International Organization for Standardization (ISO) and has been taken over as CEN ISO/TS 21268-4:2009

by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Cyprus, Czech

Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,

Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,

Sweden, Switzerland and the United Kingdom.
Endorsement notice

The text of ISO/TS 21268-4:2007 has been approved by CEN as a CEN ISO/TS 21268-4:2009 without any

modification.
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SIST-TS CEN ISO/TS 21268-4:2010
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SIST-TS CEN ISO/TS 21268-4:2010
TECHNICAL ISO/TS
SPECIFICATION 21268-4
First edition
2007-11-15
Soil quality — Leaching procedures
for subsequent chemical
and ecotoxicological testing of soil
and soil materials —
Part 4:
Influence of pH on leaching with initial
acid/base addition
Qualité du sol — Modes opératoires de lixiviation en vue d'essais
chimiques et écotoxicologiques ultérieurs des sols et matériaux du
sol —
Partie 4: Essai de dépendance au pH avec ajout initial d'acide/base
Reference number
ISO/TS 21268-4:2007(E)
ISO 2007
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SIST-TS CEN ISO/TS 21268-4:2010
ISO/TS 21268-4:2007(E)
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All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,

electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or

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Published in Switzerland
ii © ISO 2007 – All rights reserved
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SIST-TS CEN ISO/TS 21268-4:2010
ISO/TS 21268-4:2007(E)
Contents Page

Foreword............................................................................................................................................................ iv

Introduction ........................................................................................................................................................ v

1 Scope ..................................................................................................................................................... 1

2 Normative references ........................................................................................................................... 1

3 Terms and definitions........................................................................................................................... 2

4 Symbols and abbreviations ................................................................................................................. 3

5 Principle................................................................................................................................................. 4

6 Apparatus .............................................................................................................................................. 4

7 Reagents................................................................................................................................................ 6

8 Sample pretreatment ............................................................................................................................ 6

8.1 Sample size ........................................................................................................................................... 6

8.2 Particle size reduction.......................................................................................................................... 6

8.3 Determination of the dry matter content and of water content........................................................ 7

8.4 Preparation of test portion................................................................................................................... 7

9 Procedure .............................................................................................................................................. 7

9.1 Contact time .......................................................................................................................................... 7

9.2 pH-range ................................................................................................................................................ 8

9.3 Leaching test......................................................................................................................................... 8

9.3.1 General................................................................................................................................................... 8

9.3.2 Preparation of leachant........................................................................................................................ 8

9.3.3 Leaching procedure.............................................................................................................................. 9

9.4 Natural pH............................................................................................................................................ 11

10 Eluate treatment, storage and analysis............................................................................................ 11

11 Analytical determination .................................................................................................................... 12

12 Blank test............................................................................................................................................. 12

13 Calculation........................................................................................................................................... 12

14 Test report ........................................................................................................................................... 13

15 Performance characteristics ............................................................................................................. 13

Annex A (informative) Example of a specific liquid-solid separation procedure for soil sample............ 14

Annex B (informative) Operation and uses of the test: influence of pH on the leaching behaviour....... 15

Annex C (informative) Preliminary determination of the acid/base consumption..................................... 19

Bibliography ..................................................................................................................................................... 23

© ISO 2007 – All rights reserved iii
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SIST-TS CEN ISO/TS 21268-4:2010
ISO/TS 21268-4:2007(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies

(ISO member bodies). The work of preparing International Standards is normally carried out through ISO

technical committees. Each member body interested in a subject for which a technical committee has been

established has the right to be represented on that committee. International organizations, governmental and

non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the

International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.

The main task of technical committees is to prepare International Standards. Draft International Standards

adopted by the technical committees are circulated to the member bodies for voting. Publication as an

International Standard requires approval by at least 75 % of the member bodies casting a vote.

In other circumstances, particularly when there is an urgent market requirement for such documents, a

technical committee may decide to publish other types of document:

⎯ an ISO Publicly Available Specification (ISO/PAS) represents an agreement between technical experts in

an ISO working group and is accepted for publication if it is approved by more than 50 % of the members

of the parent committee casting a vote;

⎯ an ISO Technical Specification (ISO/TS) represents an agreement between the members of a technical

committee and is accepted for publication if it is approved by 2/3 of the members of the committee casting

a vote.

An ISO/PAS or ISO/TS is reviewed after three years in order to decide whether it will be confirmed for a

further three years, revised to become an International Standard, or withdrawn. If the ISO/PAS or ISO/TS is

confirmed, it is reviewed again after a further three years, at which time it must either be transformed into an

International Standard or be withdrawn.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO/TS 21268-4 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 7, Soil

and site assessment.

ISO/TS 21268 consists of the following parts, under the general title Soil quality — Leaching procedures for

subsequent chemical and ecotoxicological testing of soil and soil materials:
⎯ Part 1: Batch test using a liquid to solid ratio of 2 l/kg dry matter
⎯ Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter
⎯ Part 3: Up-flow percolation test
⎯ Part 4: Influence of pH on leaching with initial acid/base addition
iv © ISO 2007 – All rights reserved
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Introduction

In various countries, tests have been developed to characterize and assess the constituents that can be

released from materials. The release of soluble constituents upon contact with water is regarded as a main

mechanism of release, resulting in a potential risk to the environment during the use or disposal of materials.

The intent of these tests is to identify the leaching properties of materials. The complexity of the leaching

process makes simplifications necessary.

Not all of the relevant aspects of leaching behaviour can be addressed in one standard.

Tests to characterize the behaviour of materials can generally be divided into three categories (see

References [1], [2] and [4]). The relationships between these tests are summarized below.

a) “Basic characterization” tests are used to obtain information on the short- and long-term leaching

behaviour and characteristic properties of materials. Liquid/solid (L/S) ratios, leachant composition,

factors controlling leachability, such as pH, redox potential, complexing capacity, role of dissolved organic

carbon (DOC), ageing of material and physical parameters, are addressed in these defined tests.

b) “Compliance” tests are used to determine whether the material complies with a specific behaviour or with

specific reference values. These tests focus on key variables and leaching behaviour previously identified

by basic characterization tests.

c) “On-site verification” tests are used as a rapid check to confirm that the material is the same as that which

has been subjected to the compliance test(s). On-site verification tests are not necessarily leaching tests.

The test procedure described in this method belongs to category a) “Basic characterization” tests.

NOTE Up to now, the test procedures described in this part of ISO/TS 21268 have not been validated.

© ISO 2007 – All rights reserved v
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SIST-TS CEN ISO/TS 21268-4:2010
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SIST-TS CEN ISO/TS 21268-4:2010
TECHNICAL SPECIFICATION ISO/TS 21268-4:2007(E)
Soil quality — Leaching procedures for subsequent chemical
and ecotoxicological testing of soil and soil materials —
Part 4:
Influence of pH on leaching with initial acid/base addition
1 Scope

This part of ISO/TS 21268 specifies a test method to obtain information on the short- and long-term leaching

behaviour and characteristic properties of materials.

It applies to the determination of the influence of pH on the leachability of inorganic and organic constituents

from soil and soil material, and the ecotoxicological effects of eluates with respect to microorganisms, fauna

and flora. The test is not suitable for constituents that are volatile under ambient conditions. The equilibrium

condition, as defined in this part of ISO/TS 21268, is established by the addition of predetermined amounts of

acid or base to reach desired final pH values.

The test procedure specified in this part of ISO/TS 21268 produces eluates that are subsequently

characterized by physical, chemical and ecotoxicological standard methods.

For the purposes of ecotoxicological tests, the relevant pH range (see 9.2) will usually be pH 5 to 9.

NOTE 1 Volatile organic constituents include the low molecular weight components in mixtures such as mineral oil.

NOTE 2 It is not always possible to optimize test conditions simultaneously for inorganic and organic constituents and

optimum test conditions may also vary between different groups of organic constituents. Test requirements for organic

constituents are generally more stringent than those for inorganic constituents. The test conditions suitable for measuring

the release of organic constituents will generally also be applicable to inorganic constituents.

NOTE 3 For ecotoxicological testing, eluates representing the release of both inorganic and organic contaminants are

needed. In this document, ecotoxicological testing is meant to include genotoxicological testing.

This test cannot be used alone to determine the total leaching behaviour of a soil. More leaching tests are

needed for that extended goal. This part of ISO/TS 21268 does not address issues related to health and

safety. It only determines the leaching properties outlined in Clause 5.
2 Normative references

The following referenced documents are indispensable for the application of this document. For dated

references, only the edition cited applies. For undated references, the latest edition of the referenced

document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods

ISO 5667-3, Water quality — Sampling — Part 3: Guidance on the preservation and handling of water

samples
ISO 7027, Water quality — Determination of turbidity
© ISO 2007 – All rights reserved 1
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SIST-TS CEN ISO/TS 21268-4:2010
ISO/TS 21268-4:2007(E)

ISO 10381-1, Soil quality — Sampling — Part 1: Guidance on the design of sampling programmes

ISO 10381-2, Soil quality — Sampling — Part 2: Guidance on sampling techniques
ISO 10381-3, Soil quality — Sampling — Part 3: Guidance on safety

ISO 10381-4, Soil quality — Sampling — Part 4: Guidance on the procedure for investigation of natural, near-

natural and cultivated sites

ISO 10381-5, Soil quality — Sampling — Part 5: Guidance on the procedure for the investigation of urban and

industrial sites with regard to soil contamination
ISO 10523, Water quality — Determination of pH

ISO 11465, Soil quality — Determination of dry matter and water content on a mass basis — Gravimetric

method
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
leaching test

test during which a soil or soil material is put into contact with a leachant under strictly defined conditions and

some constituents of the material are extracted
3.2
leachant
liquid used in a leaching test
3.3
eluate
solution obtained by a laboratory leaching test
3.4
single batch leaching test

leaching test in which a fixed amount of material is leached in one step with a fixed amount of leachant

3.5
liquid to solid ratio
L/S
ratio between the amount of liquid (L) and of solid (S) in the test
NOTE L/S is expressed in litres per kilogram (l/kg) of dry matter.
3.6
dry matter content

ratio, expressed in percent, between the mass of the dry residue, determined in accordance with ISO 11465,

and the corresponding raw mass
3.7
water content
H O

ratio, expressed in percent, between the mass of water contained in the material as received and the

corresponding dry residue of the material.

NOTE The basis for the calculation of the moisture content is the mass of the dry residue in this part of

ISO/TS 21268, as specified in ISO 11465 (for the determination of the water content of soil).

2 © ISO 2007 – All rights reserved
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ISO/TS 21268-4:2007(E)
3.8
laboratory sample
sample or subsample(s) sent to or received by the laboratory
[IUPAC:1997]
3.9
test sample

sample, prepared from the laboratory sample, from which test portions are removed for testing or analysis

[IUPAC:1997]
3.10
test portion

amount or volume of the test sample taken for analysis, usually of known weight or volume

[IUPAC:1997]
3.11
soil material

excavated soil, dredged materials, manufactured soils, treated soil and fill materials

[ISO 15176:2002]
4 Symbols and abbreviations
ANC acid neutralization capacity
BNC base neutralization capacity
C is the concentration of the base (mol/l) (see 7.4)
DOC dissolved organic carbon
L/S liquid to solid ratio
m is the mass of the dried sample (kg)
m is the mass of non-dried sample (kg)
n is the base consumption for the particular pH (mol /kg OH dry matter)
t time at the start of the leaching test
V V volume of acid /base used in leachant
A, B
V volume of demineralized water used in leachant
V volume of prepared leachant
w dry matter content of the soil
w water content
H O
© ISO 2007 – All rights reserved 3
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ISO/TS 21268-4:2007(E)
5 Principle

Several separate test portions (up to eight) are leached at a fixed L/S ratio (L/S = 10 l/kg) with leachants

containing different preselected amounts of acid or base containing a low concentration (0,001 mol/l) of

calcium chloride in order to reach stationary pH values at the end of the extraction period (see 8.4). Each

leachant is added in three steps in the beginning of the test. In the full test eight final pH-values are required,

covering the range pH 4 to pH 12 (both included, i.e. the lowest value 4 and the highest value 12). The

amounts of acid or base needed to cover the pH range can be derived from the results of a preliminary

titration, from available experimental data on the material to be tested or from an arbitrary division of the

predetermined maximum consumption of acid and base. The tests are carried out at a fixed contact time at

the end of which an equilibrium condition can be assumed to be reached for most constituents in most soil

materials to be characterized. The equilibrium condition, as defined in this part of ISO/TS 21268, is verified at

the end of the extraction period.

The results are expressed in milligrams per litre (mg/l) of constituents for each final pH value. For each final

pH value, the quantity of acid that is added is also expressed in mol/kg H dry matter and the quantity of base

that is added is expressed as negative mol/kg H dry matter.

NOTE 1 This test can also be performed using continuous pH control. The results are generally consistent (see

Annex B).

NOTE 2 Other expressions of results are possible (including mg/kg of dry matter).

From the amount of acid and base used to reach a given end pH, the acid or base neutralization capacity

(ANC, BNC) of the soil or soil material can also be determined.

NOTE 3 The pH range covered by the test can be restricted to a pH range relevant for the specific material and the

considered problem (see 9.2).

NOTE 4 The leachant is made with 0,001 mol/l CaCl to minimize the mobilization of DOC caused by a too-low ionic

strength of the leachant. At the level of 0,001 mol/l CaCl the complexation of metals with chloride is considered to be

negligible.

The constituents in the eluate(s) are measured using methods developed for water analysis adapted to meet

criteria for analysis of eluates. The eluate may also be applied for subsequent ecotoxicity or genotoxicity

testing.

After the test, the leaching conditions (in terms of pH, electrical conductivity, DOC and, optionally, turbidity and

redox potential dictated by the material) are recorded.

NOTE 5 These parameters often control the leaching behaviour of soil materials and are therefore important for

checking the leaching test.
6 Apparatus

6.1 Borosilicate glass, of high purity in accordance with ISO 5667-3, with a nominal volume of 1 l, glass

bottles having caps of inert material, for example, PTFE (polytetrafluoroethylene). Rinsing is compulsory.

NOTE 1 If only inorganic parameters are analysed, alternative materials such as HDPE/PP bottles can be used, except

for unpreserved samples for mercury analysis.

NOTE 2 If Boron analyses are necessary, any plastics bottles may be used, e.g. PTFE (polytetrafluoroethylene).

The nominal volume of 1 l is selected in combination with the mass, m , of 60 g, in order to minimize head-

space. For m = 15 g and 30 g, bottle sizes of, respectively, 250 ml and 500 ml shall be used. In the case of

materials with low densities, a deviation from this requirement can be necessary while still attempting to

minimize headspace. This deviation should be reported.
4 © ISO 2007 – All rights reserved
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SIST-TS CEN ISO/TS 21268-4:2010
ISO/TS 21268-4:2007(E)

Glass of high quality is considered adequate for both inorganic and organic contaminants, particularly since

the pH range usually covered in soil testing does not reach the conditions (pH > 12 and pH < 4) where the

glass itself is attacked. For ecotoxicity testing, eluates with both inorganic and organic contaminants are

needed, which emphasizes the need to generate integrated eluates.
−1 −1 −1

6.2 End-over-end tumbler (5 min to 10 min ) or roller table, rotating at about 10 min .

Other shaking devices can be used, provided that they can be shown to provide equivalent results. These

agitation devices are specified for excessive abrasion leading to significant particle size reduction.

6.3 Filtration apparatus, either a vacuum filtration device (between 2,5 kPa to 4,0 kPa) or a high pressure

filtration apparatus (< 0,5 MPa). Cleaning is compulsory.

6.4 Pre-rinsed 0,45 µm membrane filters, for filtration (e.g. rinsed with 0,1 mol/l HNO as described in 7.2,

and water as described in 7.1).
1) 1)

The filters shall be glass fibre filters without organic glue or regenerated cellulose (S&S , RC 55 or

comparable quality). The filter material shall be compatible with the extractant solution to be tested.

NOTE If only inorganic contaminants are to be analysed, alternative filter materials can be selected, e.g. cellulose

acetate, PTFE.
6.5 Sieving equipment, with sieves of 2 mm nominal screen size.

NOTE Due to sieving, contamination of the sample can occur to an extent which affects the leaching of some

constituents of concern, e.g. chromium, nickel and molybdenum from stainless steel equipment or plasticizers from plastic

sieves.

6.6 Centrifuge, operating at 20 000 g to 30 000 g using centrifuge tubes of FEP (fluorinated ethylene

propylene) or tubes of an alternative material, which is inert with regard to both inorganic and organic

compounds and suitable for high-speed centrifugation.

Alternatively, if a high-speed centrifuge is not available, a centrifuge operating at 2 000 g to 2 500 g using

glass bottles may be used in combination with increased centrifugation ti
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