EN ISO 12872:2014
(Main)Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2010)
Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2010)
ISO 12872:2010 specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate content in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C).
Olivenöle und Oliventresteröle - Verfahren zur Bestimmung des Prozentanteiles von 2-Glyceryl Monopalmitat in Olivenölen (ISO 12872:2010)
Diese Internationale Norm beschreibt ein Verfahren zur Bestimmung des Gehalts, als Massenanteil in Prozent, an 2-Glycerylmonopalmitat in Olivenölen und Oliventresterölen, die bei Umgebungstemperatur (20 °C) flüssig sind.
ANMERKUNG Diese Internationale Norm basiert auf COI/T.20/Doc. 23:2006 [6].
Huiles d'olive et huiles de grignons d'olive - Détermination de la teneur en 2-glycéryl monopalmitate (ISO 12872:2010)
L'ISO 12872:2010 spécifie une méthode pour la détermination de la teneur, sous forme de fraction massique en pourcentage, en 2-glycéryl monopalmitate des huiles d'olive et des huiles de grignons d'olive liquides à température ambiante (20 °C).
Oljčna olja in olja iz oljčnih tropin - Določevanje vsebnosti 2-glyceryl monopalmitata (ISO 12872:2010)
Standard EN ISO 12872 navaja postopek za določevanje vsebnosti 2-gliceril monopalmitata kot odstotek masnega deleža v olivnih oljih in oljih iz oljčnih tropin, ki so pri temperaturi okolja (20° C) v tekočem stanju.
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.O\FHU\OOlivenöle und Oliventresteröle - Verfahren zur Bestimmung des Prozentanteiles von 2-Glyceryl Monopalmitat in Olivenölen (ISO 12872:2010)Huiles d'olive et huiles de grignons d'olive - Détermination de la teneur en 2-glycéryl monopalmitate (ISO 12872:2010)Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2010)67.200.10Animal and vegetable fats and oilsICS:Ta slovenski standard je istoveten z:EN ISO 12872:2014SIST EN ISO 12872:2014en01-julij-2014SIST EN ISO 12872:2014SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN ISO 12872
April 2014 ICS 67.200.10 English Version
Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2010)
Huiles d'olive et huiles de grignons d'olive - Détermination de la teneur en 2-glycéryl monopalmitate (ISO 12872:2010) Olivenöle und Oliventresteröle - Verfahren zur Bestimmung des Prozentanteiles von 2-Glyceryl Monopalmitat in Olivenölen (ISO 12872:2010) This European Standard was approved by CEN on 11 April 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
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Reference numberISO 12872:2010(E)© ISO 2010
INTERNATIONAL STANDARD ISO12872First edition2010-05-01Olive oils and olive-pomace oils — Determination of the 2-glyceryl monopalmitate content Huiles d'olive et huiles de grignons d'olive — Détermination de la teneur en 2-glycéryl monopalmitate
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ii © ISO 2010 – All rights reserved
ISO 12872:2010(E) © ISO 2010 – All rights reserved iii Contents Page Foreword.iv Introduction.v 1 Scope.1 2 Normative references.1 3 Terms and definitions.1 4 Principle.1 5 Reagents.1 6 Apparatus.2 7 Sampling.3 8 Preparation of the test sample.3 9 Procedure.4 9.1 Preparatory steps.4 9.2 Column chromatography.4 9.3 Hydrolysis with pancreatic lipase.4 9.4 Preparation of the silylated derivatives and gas chromatography.5 9.5 Gas chromatography.5 10 Expression of results.5 11 Precision.6 11.1 Interlaboratory test.6 11.2 Repeatability.6 11.3 Reproducibility.6 12 Test report.6 Annex A (informative)
Chromatograms.7 Annex B (informative)
Results of an interlaboratory test.10 Annex C (informative)
Preparation and activity of the lipase.11 Bibliography.13
ISO 12872:2010(E) iv © ISO 2010 – All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 12872 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal and vegetable fats and oils. SIST EN ISO 12872:2014
ISO 12872:2010(E) © ISO 2010 – All rights reserved v Introduction As part of the Trade standard applying to olive oils and olive-pomace oils, the International Olive Council (IOC) published COI/T.20/Doc. 23:2006[6]. COI/T.20/Doc. 23 was applicable to olive and olive-pomace oils and was used to distinguish between lampante virgin olive oils and crude olive-pomace oils. Olive pomace is the residual paste which still contains a variable amount of water and oil after pressing or centrifuging. In 2008, the IOC submitted the document to ISO/TC 34/SC 11 for adoption as an International Standard.
INTERNATIONAL STANDARD ISO 12872:2010(E) © ISO 2010 – All rights reserved 1 Olive oils and olive-pomace oils — Determination of the
2-glyceryl monopalmitate content 1 Scope This International Standard specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C). NOTE This International Standard is based on COI/T.20/Doc. 23:2006[6]. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 661, Animal and vegetable fats and oils — Preparation of test sample 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 2-glyceryl monopalmitate content mass fraction of 2-glyceryl monopalmitate in the monoacylglycerol fraction, determined according to the method specified in this International Standard NOTE The 2-glyceryl monopalmitate content is expressed as a percentage. 4 Principle The oil, after suitable preparation, is subjected to the action of pancreatic lipase. A partial hydrolysis takes place that is specific for positions 1 and 3 of the triacylglycerol molecule so that 2-monoacylglycerols are obtained as reaction products. The percentage of 2-glyceryl monopalmitate in the monoacylglycerol fraction is determined, after silylation, by capillary gas chromatography. 5 Reagents WARNING — Comply with any local regulations which specify the handling of hazardous substances. Technical, organizational and personal safety measures shall be followed. During the analysis, unless otherwise stated, use only reagents of recognized analytical grade, and distilled or demineralized water or water of equivalent purity. SIST EN ISO 12872:2014
ISO 12872:2010(E) 2 © ISO 2010 – All rights reserved 5.1 Silica gel, with a particle size of 0,063 mm to 0,200 mm (70/280 mesh), prepared as follows: put the silica gel into a porcelain cup, dry in an oven at 160 °C for 4 h, then cool at ambient temperature in a desiccator. Add a volume of water equivalent to 5 % of the mass of the silica gel as follows: weigh 152 g of silica gel into a 500 ml Erlenmeyer flask, add 8 g of water, stopper and homogenize carefully. Leave to settle for at least 12 h before using. 5.2 n-Hexane, chromatography grade. 5.3 Isopropanol. 5.4 Isopropanol-water mixture, volume fractions 50 ml/100 ml. 5.5 Pancreatic lipase, activity between 2,0 a
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