prEN 18236

Pulp, paper and board - Determination of the migration of polycyclic aromatic hydrocarbons (PAH) into food simulants

This document specifies a test method for the determination of the specific migration of polycyclic aromatic hydrocarbons (PAH) from paper, board and pulp into the food simulant isooctane. The amounts of polycyclic aromatic hydrocarbons (PAHs) are expressed in micrograms PAH per litre of solvent extract.
It is applicable for the determination of the 24 PAHs given in 6.3, Table 1. The test method is also applicable for the determination of further PAHs unless a validation for every further analyte is done by the laboratory using this test method.
In general, this test method is applicable to the determination of PAHs in concentrations ranging from 2,5 μg/l to 100 μg/l for solvent extracts. Deviations from this range are possible.

Faserstoff, Papier und Pappe - Bestimmung der Migration von polyzyklischen aromatischen Kohlenwasserstoffen (PAK) in Lebensmittelsimulanzien

Dieses Dokument legt ein Prüfverfahren für die Bestimmung der spezifischen Migration polyzyklischer aro
matischer Kohlenwasserstoffe(PAK) aus Papier, Pappe und Faserstoff in das Lebensmittelsimulanz Isooktan fest. Die Menge an polyzyklischen aromatischen Kohlenwasserstoffen(PAK) wird in Mikrogramm PAK je Liter Lösemittelextrakt angegeben.
Es ist anwendbar für die Bestimmung der 24PAK, die in6.3, Tabelle1 angegeben sind. Das Prüfverfahren ist ebenfalls anwendbar für die Bestimmung weiterer PAK, sofern das Laboratorium, das dieses Prüfverfahren anwendet, für jeden weiteren Analyten eine Validierung durchführt.
Dieses Prüfverfahren ist im Allgemeinen anwendbar für die Bestimmung von PAK in Konzentrationen von 2,5μg/l bis 100μg/l in Lösemittelextrakten. Abweichungen von diesem Bereich sind möglich.

Pâtes, papier et carton - Détermination de la migration des hydrocarbures aromatiques polycycliques (HAP) dans les simulants de denrées alimentaires

Le présent document spécifie une méthode d’essai pour la détermination de la migration spécifique d’hydrocarbures aromatiques polycycliques (HAP) provenant du papier, du carton et des pâtes à papier dans l’isooctane utilisé en tant que simulant de denrées alimentaires. Les quantités d’hydrocarbures aromatiques polycycliques (HAP) sont exprimées en microgrammes d’HAP par litre d’extrait de solvant.
La méthode d’essai est applicable à la détermination de 24 HAP indiqués en 6.3, Tableau 1. Elle est également applicable à la détermination d’autres HAP, sauf si le laboratoire utilisant cette méthode d’essai procède à une validation pour chaque analyte supplémentaire.
En règle générale, cette méthode d’essai est applicable à la détermination d’HAP dont la concentration est comprise entre 2,5 μg/l et 100 μg/l d’extrait de solvant. Des écarts par rapport à cette plage sont possibles.

Celuloza, papir in karton - Ugotavljanje migracije policikličnih aromatskih ogljikovodikov (PAH) v simulante živil

General Information

• Status: Not Published
• Publication Date: 15-Aug-2027
• ICS: 67.250 - Materials and articles in contact with foodstuffs
• ICS: 85.060 - Paper and board
• Technical Committee: CEN/TC 172 - Pulp, paper and board
• Drafting Committee: CEN/TC 172/WG 3 - Analytical methods for the assessment of paper and board in contact with foodstuffs
• Current Stage: 4020 - Submission to enquiry - Enquiry
• Start Date: 02-Oct-2025
• Due Date: 25-Jun-2025
• Completion Date: 02-Oct-2025
• Ref Project: oSIST prEN 18236:2025 - Pulp, paper and board - Determination of the migration of polycyclic aromatic hydrocarbons (PAH) into food simulants

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2025
Celuloza, papir in karton - Ugotavljanje migracije policikličnih aromatskih
ogljikovodikov (PAH) v simulante živil
Pulp, paper and board - Determination of the migration of polycyclic aromatic
hydrocarbons (PAH) into food simulants
Faserstoff, Papier und Pappe - Bestimmung der Migration von polyzyklischen
aromatischen Kohlenwasserstoffen (PAK) in Lebensmittelsimulanzien
Pâtes, papier et carton - Détermination de la migration des hydrocarbures aromatiques
polycycliques (HAP) dans les simulants de denrées alimentaires
Ta slovenski standard je istoveten z: prEN 18236
ICS:
67.250 Materiali in predmeti v stiku z Materials and articles in
živili contact with foodstuffs
85.060 Papir, karton in lepenka Paper and board
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
EUROPEAN STANDARD
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 67.250; 85.060
English Version
Pulp, paper and board - Determination of the migration of
polycyclic aromatic hydrocarbons (PAH) into food
simulants
Pâtes, papier et carton - Détermination de la migration Faserstoff, Papier und Pappe - Bestimmung der
des hydrocarbures aromatiques polycycliques (HAP) Migration von polyzyklischen aromatischen
dans les simulants de denrées alimentaires Kohlenwasserstoffen (PAK) in
Lebensmittelsimulanzien
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 172.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 18236:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
1 Scope . 4
2 Normative references . 4
3 Terms and definitions . 4
4 Principle . 4
4.1 General. 4
4.2 Interferences . 4
5 Apparatus and auxiliary aids . 5
6 Reagents . 5
7 Sampling . 7
8 Preparation of the test isooctane extract . 7
9 Procedure . 7
9.1 Extra work-up . 7
9.1.1 Addition of internal standard solution . 7
9.1.2 Pre-concentration and clean-up . 7
9.1.3 Blank value . 8
9.2 GC conditions . 8
9.2.1 General. 8
9.2.2 Example of GC conditions . 8
9.2.3 Example of MS conditions . 8
9.3 Calibration . 10
9.4 Determination of PAHs in the sample solutions . 11
9.4.1 General. 11
9.4.2 Interferences . 11
9.5 Calculation of the PAH concentration in the sample extracts. 11
9.6 Precision of the method . 11
10 Test report . 12
Annex A (informative) Precision data . 13
Annex B (informative) Pre-concentration and clean-up . 14

European foreword
This document (prEN 18236:2025) has been prepared by Technical Committee CEN/TC 172 “Pulp, paper
and board”, the secretariat of which is held by DIN.
This document is currently submitted to the CEN Enquiry.
1 Scope
This document specifies a test method for the determination of the specific migration of polycyclic
aromatic hydrocarbons (PAH) from paper, board and pulp into the food simulant isooctane. The amounts
of polycyclic aromatic hydrocarbons (PAHs) are expressed in micrograms PAH per litre of solvent extract.
It is applicable for the determination of the 24 PAHs given in 6.3, Table 1. The test method is also
applicable for the determination of further PAHs unless a validation for every further analyte is done by
the laboratory using this test method.
In general, this test method is applicable to the determination of PAHs in concentrations ranging from
2,5 µg/l to 100 µg/l for solvent extracts. Deviations from this range are possible.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 15519, Paper and board intended to come into contact with foodstuffs — Preparation of an organic
solvent extract
EN ISO 186, Paper and board — Sampling to determine average quality (ISO 186)
EN ISO 4787, Laboratory glass and plastic ware — Volumetric instruments — Methods for testing of
capacity and for use (ISO 4787)
EN ISO 7213, Pulps — Sampling for testing (ISO 7213)
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp/
— IEC Electropedia: available at https://www.electropedia.org/
4 Principle
4.1 General
A sample extract is prepared by means of an organic solvent (isooctane) according to EN 15519. The
determination of the analytes is performed by gas chromatography/mass spectrometry (GC-MS) using
anthracene-d and perylene-d as internal standards.
10 12
4.2 Interferences
— As PAHs are sensitive to UV light, it shall be ensured that the samples are protected from light at all
stages of treatment. This can be achieved by e.g. using brown glassware or intermediate storing of
the samples in the dark.
— Portions of organic acids and of phthalic- and fatty acid esters, the solvents from carbon-less copy
papers (e.g. diisopropylnaphtalene) and mineral oil hydrocarbons can interfere the analysis.
5 Apparatus and auxiliary aids
The usual laboratory apparatus and laboratory glassware, including brown glass, according to
EN ISO 4787, shall be used, in addition to the following:
5.1 Balance with a resolution of at least 0,000 1 g.
5.2 Gas chromatograph coupled to a mass spectrometer (GC-MS) (EI+).
6 Reagents
All reagents shall be of recognized analytical quality unless stated otherwise.
6.1 Operating gases for GC-MS, shall be of high purity and in accordance with manufacturer’s
specifications.
6.2 Isooctane, CAS Registry Number® (CAS RN® 540-84-1), with purity of 98,5 % or better.
6.3 Reference substances
Table 1 — List of reference substances
No. Substance Sum CAS RN® Concentration of the
formula ready-to-use solution
ng/µl
1 Naphthalene C H 91–20–3
10 8
2 Acenaphthylene C H 208–96–8
12 8
3 Acenaphthene C H 83–32–9
12 10
4 Fluorene C H 86–73–7
13 10
5 Phenanthrene C H 08–01–85
14 10
6 Anthracene C H 120–12–7
14 10
7 Fluoranthene C H 206–44–0
16 10
8 Pyrene C16H10 129–00–0
9 Benz[a]anthracene C18H 56–55–3
10 Chrysene C H 218–01–9
18 12
11 Benzo[b]fluoranthene C H 205–99–2
20 12
12 Benzo[k]fluoranthene C H 207–08–9
20 12
13 Benzo[a]pyrene C H 50–32–8
20 12
14 Dibenz[a,h]anthracene C H 53–70–3
22 14
15 Benzo[g,h,i]perylene C H 191–24–2
22 12
16 Indeno[1,2,3-cd]pyrene C H 193–39–5
22 12
1 ® ®
CAS Registry Number (CAS RN ) is a trademark American Chemical Society (ACS). This information is given for
the convenience of users of this document and does not constitute an endorsement by CEN of the product named.
Equivalent products may be used if they can be shown to lead to the same results.
No. Substance Sum CAS RN® Concentration of the
formula ready-to-use solution
ng/µl
17 2-Methylnaphthalene C H 91–57–6
11 10
18 1-Methylnaphthalene C H 90–12–0
11 10
19 2-Methylphenanthrene C H 2531–84–2
15 12
20 Benzo[c]phenanthrene C H 195–19–7
18 12
21 Benzo[b]naphtho[1,2-d]thiophene C H S 205–43–6
16 10
22 Benzo[e]pyrene C H 192–97–2
20 12
23 Perylene C H 198–55–0
20 12
24 Triphenylene C H 217–59–4
18 12
6.4 Internal standards
6.4.1 List of internal standards
Table 2 — List of internal standards
No. Substance Sum CAS RN® Concentration of the
formula ready-to-use solution
ng/µl
25 Anthracene-d10 C14D10 1719–06–8
26 Perylene-d C D 1520–96–3
12 20 12
The standards may be dissolved or obtained as pure substances, which are commercially available.
6.4.2 Internal standards stock solution: 10 mg/l for each individual substance
Certified solutions of anthracene-d and perylene-d (see Table 2) with a concentration of 10 mg/l are
10 12
commercially available.
6.4.3 Diluted internal standard stock solution: 0,8 mg/l for each individual substance
Transfer 2 ml of each stock solution of anthracene-d and perylene-d (6.4.2) into a 25 ml volumetric
10 12
flask and dilute to the mark with isooctane (6.2).
6.5 Calibration stock solution: individual substances
6.5.1 Stock solution of 16 PAHs: 100 mg/l for each individual substance
6.5.1.1 General
A certified mixture of the compounds 1 to 16 (see Table 1) with a concentration of 100 mg/l for each
compound is commercially available.
6.5.1.2 Diluted stock solution (A): 1 mg/l for each individual substance
Transfer 1 ml of the 100 mg/l stock solution (6.5.1) into a 100 ml volumetric flask and dilute to the mark
with isooctane (6.2).
6.5.2 Stock solution of 8 PAHs: 10 mg/l for each individual substance
...