EN ISO 20743:2021
(Main)Textiles - Determination of antibacterial activity of textile products (ISO 20743:2021)
Textiles - Determination of antibacterial activity of textile products (ISO 20743:2021)
This document specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.
This document is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).
This document covers three inoculation methods for the determination of antibacterial activity:
a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);
b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);
c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).
NOTE Based on the intended application and on the environment in which the textile product is to be used, and also on the surface properties of the textile properties, the user can select the most suitable inoculation method.
This document also specifies the colony plate count method and the adenosine triphosphate (ATP) luminescence method for measuring the enumeration of bacteria.
Textilien - Bestimmung der antibakteriellen Wirksamkeit von textilen Produkten (ISO 20743:2021)
Dieses Dokument legt quantitative Prüfverfahren zur Bestimmung der antibakteriellen Wirkung aller antibak¬teriell behandelten textilen Erzeugnisse, einschließlich Vliesstoffe, fest.
Dieses Dokument ist anwendbar auf alle textilen Erzeugnisse, einschließlich Stoffe, Wattierung, Garn und Materialien für Bekleidung, Bettwäsche, Heimtextilien sowie verschiedene Waren, ungeachtet des Typs des verwendeten antibakteriellen Wirkstoffs (organisch, anorganisch, natürlich oder künstlich) oder des Anwendungsverfahrens (eingebaut, Nachbehandlung oder Pfropfen).
Dieses Dokument behandelt drei Inokulationsverfahren zur Bestimmung der antibakteriellen Wirkung:
a) Absorptionsverfahren (ein Bewertungsverfahren, bei dem die bakterielle Prüfsuspension direkt auf Proben geimpft wird);
b) Übertragungsverfahren (ein Bewertungsverfahren, bei dem Prüfbakterien auf eine Agarplatte aufge¬bracht und auf Proben übertragen werden);
c) Stempelverfahren (ein Bewertungsverfahren, bei dem Prüfbakterien auf ein Filter aufgebracht und auf Proben gestempelt werden).
ANMERKUNG Auf der Grundlage der beabsichtigten Anwendung und der Umgebung, in der das textile Erzeugnis zu verwenden ist, und auch auf Grundlage der Oberflächeneigenschaften der Textilien, kann der Anwender das am besten geeignete Inokulationsverfahren auswählen.
Dieses Dokument legt auch das Kolonie Plattenzählverfahren und das Adenosintriphosphat (ATP)-Lumines¬zenzverfahren zum Messen der Bakterienzahl fest.
Textiles - Détermination de l'activité antibactérienne des produits textiles (ISO 20743:2021)
Le présent document spécifie des méthodes d’essai quantitatives permettant de déterminer l’activité antibactérienne de tous les produits textiles antibactériens, y compris les nontissés.
Le présent document s’applique à tous les produits textiles, y compris l’étoffe, le rembourrage, le fil et les matériaux utilisés pour les vêtements, la literie, l’ameublement et divers articles, quel que soit le type d’agent antibactérien utilisé (organique, inorganique, naturel ou synthétique) ou quelle que soit la méthode d’application (intégration, post-traitement ou greffage).
Le présent document traite de trois méthodes d’ensemencement pour la détermination de l’activité antibactérienne:
a) la méthode par absorption (méthode d’évaluation dans laquelle la suspension bactérienne d’essai est ensemencée directement sur des éprouvettes);
b) la méthode par transfert (méthode d’évaluation dans laquelle les bactéries d’essai sont placées sur une boîte de milieu gélosé et transférées sur des éprouvettes);
c) la méthode par impression (méthode d’évaluation dans laquelle les bactéries d’essai sont placées sur un filtre et imprimées sur des éprouvettes).
NOTE En tenant compte de l’application prévue et de l’environnement dans lequel le produit textile est destiné à être utilisé, et également des propriétés de surface du textile, l’utilisateur peut choisir la méthode d’ensemencement la plus adaptée.
Le présent document spécifie également la méthode par comptage sur plaque et la méthode par luminescence de l’adénosine triphosphate (ATP) pour le dénombrement des bactéries.
Tekstilije - Ugotavljanje protibakterijske aktivnosti na tekstilnih izdelkih (ISO 20743:2021)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2021
Nadomešča:
SIST EN ISO 20743:2013
Tekstilije - Ugotavljanje protibakterijske aktivnosti na tekstilnih izdelkih (ISO
20743:2021)
Textiles - Determination of antibacterial activity of textile products (ISO 20743:2021)
Textilien - Bestimmung der antibakteriellen Wirksamkeit von textilen Produkten (ISO
20743:2021)
Textiles - Détermination de l'activité antibactérienne des produits textiles (ISO
20743:2021)
Ta slovenski standard je istoveten z: EN ISO 20743:2021
ICS:
07.100.99 Drugi standardi v zvezi z Other standards related to
mikrobiologijo microbiology
59.080.01 Tekstilije na splošno Textiles in general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 20743
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2021
EUROPÄISCHE NORM
ICS 07.100.99; 59.080.01 Supersedes EN ISO 20743:2013
English Version
Textiles - Determination of antibacterial activity of textile
products (ISO 20743:2021)
Textiles - Détermination de l'activité antibactérienne Textilien - Bestimmung der antibakteriellen
des produits textiles (ISO 20743:2021) Wirksamkeit von textilen Produkten (ISO 20743:2021)
This European Standard was approved by CEN on 4 June 2021.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20743:2021 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 20743:2021) has been prepared by Technical Committee "NA" in collaboration
with Technical Committee CEN/TC 248 “Textiles and textile products” the secretariat of which is held
by BSI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2021, and conflicting national standards
shall be withdrawn at the latest by December 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 20743:2013.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 20743:2021 has been approved by CEN as EN ISO 20743:2021 without any modification.
INTERNATIONAL ISO
STANDARD 20743
Third edition
2021-06
Textiles — Determination of
antibacterial activity of textile
products
Textiles — Détermination de l'activité antibactérienne des produits
textiles
Reference number
ISO 20743:2021(E)
©
ISO 2021
ISO 20743:2021(E)
© ISO 2021
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ii © ISO 2021 – All rights reserved
ISO 20743:2021(E)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Safety precaution . 2
5 Apparatus . 2
6 Reagents and culture media . 4
6.1 Water . 4
6.2 Tryptone soya broth (TSB) . 4
6.3 Tryptone soya agar (TSA) . 4
6.4 Agar for transfer . 5
6.5 Nutrient broth (NB) . 5
6.6 Peptone salt solution . 5
6.7 Physiological saline . 5
6.8 Soybean-Casein Digest Broth with Lecithin & Polysorbate 80 (SCDLP) medium . 5
6.9 Dilution buffer for shake-out bacterial suspension. 6
6.10 Neutralizing solution . 6
6.11 Enumeration agar (EA) . 6
6.12 Agar for printing . 7
6.13 Cryoprotective solution for bacterial species . 7
6.14 Stock solution of ATP standard reagent . 7
6.15 Buffer solution for ATP luminescent reagent . 7
6.16 ATP luminescent reagent . 8
6.17 ATP extracting reagent . 8
6.18 ATP eliminating reagent . 8
6.19 SCDLP or other medium for preparing ATP reference solution . 9
6.20 Shake-out physiological saline . 9
7 Reference strains . 9
7.1 Strains . 9
7.2 Restoration and storage of strains . 9
7.2.1 General. 9
7.2.2 Ceramic bead method . 9
7.2.3 Glycerol suspension method.10
8 Test procedures .10
8.1 Absorption method (see Annex E) .10
8.1.1 Incubation of test strain .10
8.1.2 Preparation of test inoculum .11
8.1.3 Preparation of test specimens .11
8.1.4 Test operation.12
8.1.5 Test results .13
8.2 Transfer method (see Annex E) .15
8.2.1 Preparation of test inoculum .15
8.2.2 Preparation of specimens .15
8.2.3 Test operation.15
8.2.4 Test results .16
8.3 Printing method (see Annex E) .18
8.3.1 Incubation of test strain .
...
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