prEN ISO 17805
(Main)Water quality - Sampling, capture and preservation of environmental DNA from water (ISO/DIS 17805:2025)
Water quality - Sampling, capture and preservation of environmental DNA from water (ISO/DIS 17805:2025)
This document specifies procedures for sampling, capture and preservation of environmental DNA (eDNA) in aquatic environments, stemming from organisms that are or have recently been present in a waterbody, have visited it or whose DNA has been introduced to the waterbody through some mechanism. This document also covers procedures for avoiding sample contamination and ensuring DNA quality, key properties of the filtering procedure and equipment and reporting standards. This document does not include the collection of eDNA from biofilms, sediments or similar sample types and does not cover sampling designs.
Wasserbeschaffenheit - Probenahme, Erfassung und Konservierung von Umwelt-DNA aus Wasser (ISO/DIS 17805:2025)
Dieses Dokument legt Verfahren für die Probenahme, die Erfassung und die Konservierung von Umwelt-DNA (eDNA, en: environmental DNA) in Gewässern fest, die von Organismen stammen, die sich in einem Gewässer aufhalten oder aufgehalten haben, das Gewässer besucht haben oder deren DNA durch irgendeinen Mechanismus in das Gewässer gelangt ist. Dieses Dokument behandelt auch Verfahren zur Vermeidung von Probenkontaminationen und zur Sicherstellung der DNA-Qualität, Schlüsseleigenschaften des Filtrationsverfahrens und der Ausrüstung sowie Berichtsstandards.
Dieses Dokument befasst sich nicht mit der Gewinnung von eDNA aus Biofilmen, Sedimenten oder ähnlichen Probenarten oder passive Probenahmeverfahren und geht nicht auf die Planung der Probenahme ein.
Qualité de l’eau - Techniques de récolte et conservation de l’ADN environnemental à partir d’échantillons d’eau (ISO/DIS 17805:2025)
Kakovost vode - Vzorčenje, zbiranje in konzerviranje okoljske DNK iz vode (ISO/DIS 17805:2025)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2025
Kakovost vode - Vzorčenje, zbiranje in konzerviranje okoljske DNK iz vode
(ISO/DIS 17805:2025)
Water quality - Sampling, capture and preservation of environmental DNA from water
(ISO/DIS 17805:2025)
Wasserbeschaffenheit - Probenahme, Erfassung und Konservierung von Umwelt-DNA
aus Wasser (ISO/DIS 17805:2025)
Qualité de l’eau - Techniques de récolte et conservation de l’ADN environnemental à
partir d’échantillons d’eau (ISO/DIS 17805:2025)
Ta slovenski standard je istoveten z: prEN ISO 17805
ICS:
13.060.45 Preiskava vode na splošno Examination of water in
general
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
DRAFT
International
Standard
ISO/DIS 17805.2
ISO/TC 147/SC 5
Water quality — Sampling, capture
Secretariat: DIN
and preservation of environmental
Voting begins on:
DNA from water
2025-02-04
ICS: 13.060.70
Voting terminates on:
2025-04-01
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Reference number
ISO/DIS 17805.2:2025(en)
DRAFT
ISO/DIS 17805.2:2025(en)
International
Standard
ISO/DIS 17805.2
ISO/TC 147/SC 5
Water quality — Sampling, capture
Secretariat: DIN
and preservation of environmental
Voting begins on:
DNA from water
ICS: 13.060.70
Voting terminates on:
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2025
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
CP 401 • Ch. de Blandonnet 8
TO SUBMIT, WITH THEIR COMMENTS,
CH-1214 Vernier, Geneva
NOTIFICATION OF ANY RELEVANT PATENT
Phone: +41 22 749 01 11
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ISO/DIS 17805.2:2025(en)
ii
ISO/DIS 17805.2:2025(en)
Contents Page
European foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Procedure . 3
5.1 General .3
5.2 Considerations prior to fieldwork .4
5.3 Equipment preparation prior to fieldwork .4
5.4 Sampling the eDNA from water .4
5.5 Preserving the sample .5
5.5.1 General .5
5.5.2 Preserving eDNA in enclosed filters .5
5.5.3 Preserving eDNA in open filters .5
5.5.4 Preserving eDNA in housed filters .6
6 Equipment . 6
7 Preservative solutions . 7
7.1 General .7
7.2 Examples of preservative solutions .8
8 Sampling report . 8
8.1 General .8
8.2 Sample identity and characteristics .8
8.3 Sampling site .8
8.4 Sampling conditions .8
8.5 Sampling .9
9 Avoiding sample contamination . . 9
9.1 General .9
9.2 Contamination avoidance .9
9.2.1 Contamination that originates from equipment .9
9.2.2 Contamination that originates from the person taking the samples .9
9.3 Sampling equipment decontamination procedure .10
9.3.1 General .10
9.3.2 Materials and equipment in direct contact with the water sample .10
9.3.3 Materials and equipment not in direct contact with the water sample .10
Annex A (informative) Filter types .11
Bibliography .12
iii
ISO/DIS 17805.2:2025(en)
European foreword
This document (FprEN 17805:2022) has been prepared by Technical Committee CEN/TC 230 “Water
analysis”, the secretariat of which is held by DIN.
This document is currently submitted to the Formal Vote.
Any feedback and questions on this document should be directed to the users’ national standards body. A
complete listing of these bodies can be found on the CEN website.
iv
ISO/DIS 17805.2:2025(en)
Introduction
WARNING — Persons using this document should be familiar with water sampling protocols to
assess biological diversity. This document does not purport to address all of the safety problems, if
any, associated with its use. It is the responsibility of the user to establish appropriate health and
safety practices.
Moreover, the need of notification, obtaining certificates or permits prior to sampling, depending
on national or international laws and regulations such as the Nagoya Protocol on Access to Genetic
Resources (https://www.cbd.int/abs/), needs to be considered.
The monitoring of organisms is key to the assessment of the status of aquatic ecosystems and is required by
national and international legislation such as the European Union Water Framework Directive (2000/60/
EC). A range of methods have been described how to monitor organisms in aquatic environments, leading to a
wide range of European standards (e.g. EN 14011:2003, EN 14757:2005, EN 15460:2007). These approaches,
however, necessitate the capture and/or collection of the organisms of interest, which can be a laborious
and time-consuming process.
The possibility to detect the presence of organisms and/or quantify relative abundance (e.g [6].) in aquatic
environments via the analysis of environmental DNA (eDNA) provides a novel means to monitor biodiversity
[7][8][9]
across a wide range of taxonomic groups, including microorganisms, plants and animals.( ) This
approach allows to examine organismic diversity without the need to directly isolate and capture organisms
and it is expected to play a key role for future biomonitoring aiming at temporally and spatially highly
[10] [11]
resolved species inventories. Albeit the power of the eDNA approach has been repeatedly reported,
[12]
there is a great need for standardizing the application of eDNA-based assessment of aquatic biodiversity.(
[13]
) Note, however, that eDNA-based biomonitoring currently does not allow to obtain certain population
parameters (e.g. individual size, sex) which can be obtained by traditional sampling techniques.
This document provides guidance how to sample and preserve eDNA from water samples, addressing the
first and crucial step for any further downstream eDNA-based analyses of biodiversity. A specific technical
report for the routine sampling of benthic diatoms from rivers and lakes adapted for metabarcoding analyses
is CEN/TR 17245:2018.
In this document, the following verbal forms are used to specify working procedures:
— “shall” indicates a requirement;
— “should” indicates a recommendation;
— “may” indicates a permission;
— “can” indicates a possibility or a capability.
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.