ISO 22634-2:2017

Cigarettes — Determination of benzo[a]pyrene in cigarette mainstream smoke using GC/MS — Part 2: Method using cyclohexane as extraction solvent

ISO 22634-2:2017 specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent. This method was validated using ISO 3308 smoking parameters and is technically compatible with other smoking regimes. ISO 22634-2:2017 provides an alternative method to that specified in ISO 22634-1, with a different clean-up, which is less time consuming and with a reduced analytical run allowing a potential increase of sample throughput in comparison with ISO 22634-1.

Cigarettes — Dosage du benzo[a]pyrène dans le courant principal de la fumée de cigarette par CG/SM — Partie 2: Méthode utilisant du cyclohexane comme solvant d'extraction

ISO 22634-2:2017 spécifie une méthode de dosage du benzo[a]pyrène (B[a]P) dans la matière particulaire totale du courant principal de la fumée de cigarette par chromatographie en phase gazeuse couplée à la spectrométrie de masse (CG/SM) en utilisant du cyclohexane comme solvant d'extraction. Cette méthode a été validée en utilisant les paramètres de fumage de l'ISO 3308, et elle est techniquement compatible avec d'autres régimes de fumage. ISO 22634-2:2017 définit une méthode alternative à l'ISO 22634-1, avec un nettoyage différent, moins chronophage et avec une série d'analyses réduite, qui permet une augmentation potentielle de la cadence des échantillons par comparaison à l'ISO 22634-1.

General Information

• Status: Withdrawn
• Publication Date: 30-Nov-2017
• Withdrawal Date: 30-Nov-2017
• ICS: 65.160 - Tobacco, tobacco products and related equipment
• Technical Committee: ISO/TC 126 - Tobacco and tobacco products
• Drafting Committee: ISO/TC 126 - Tobacco and tobacco products
• Current Stage: 9599 - Withdrawal of International Standard
• Start Date: 04-Dec-2019
• Completion Date: 17-May-2025

Relations

• Revised: ISO 22634-2:2019 - Cigarettes — Determination of benzo[a]pyrene in cigarette mainstream smoke using GC/MS — Part 2: Method using cyclohexane as extraction solvent
• Effective Date: 30-Jun-2018
• Revised: ISO 22634:2008 - Cigarettes — Determination of benzo[a]pyrene in cigarette mainstream smoke — Method using gas chromatography/mass spectrometry
• Effective Date: 02-Mar-2013

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 22634-2
First edition
2017-11
Cigarettes — Determination
of benzo[a]pyrene in cigarette
mainstream smoke using GC/MS —
Part 2:
Method using cyclohexane as
extraction solvent
Cigarettes — Dosage du benzo[a]pyrène dans le courant principal de
la fumée de cigarette par CG/SM —
Partie 2: Méthode utilisant du cyclohexane comme solvant
d'extraction
Reference number
©
ISO 2017
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. [phone]
Fax [phone]
[e-mail]
www.iso.org
ii © ISO 2017 – All rights reserved

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus . 2
6 Reagents . 2
7 Standards . 3
7.1 General . 3
7.2 Primary B[a]P-d12 stock solution: 100 µg/ml . 3
7.3 Secondary B[a]P-d12 spiking solution: 40 ng/ml . 3
7.4 Primary B[a]P stock solution: 100 µg/ml . 3
7.5 Secondary B[a]P stock solution: 1 000 ng/ml . 3
7.6 Working standard solutions . 3
7.7 Storage of standard solutions. 3
8 Preparation of sample . 3
8.1 Sampling . 3
8.2 Smoking . 4
8.3 Filter pad extraction . 4
8.4 Sample clean-up . 4
8.5 Blank solution . 5
9 Determination . 5
9.1 GC/MS operating conditions . 5
9.2 Calibration . 5
9.3 Determination of B[a]P . 6
9.4 Calculation . 6
10 Repeatability and reproducibility . 6
11 Test report . 7
11.1 General . 7
11.2 Characteristic data about the cigarette . 7
11.3 Data about sampling . 7
11.4 Description of the test . 8
11.5 Test results. 8
Annex A (informative) Example of a chromatogram of a cigarette smoke extract .9
Bibliography .10
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
A list of all parts in the ISO 22634 series can be found on the ISO website.
iv © ISO 2017 – All rights reserved

Introduction
No machine smoking regime can represent all human smoking behaviours:
— it is recommended that cigarettes also be tested under conditions of a different intensity of machine
smoking than those specified in this document;
— machine smoking testing is useful to characterize cigarette emissions for design and regulatory
purposes, but communication of machine measurements to smokers can result in misunderstandings
about differences in exposure and risk across brand;
— smoke emission data from machine measurements may be used as inputs for product hazard
assessment, but they are not intended to be nor are they valid measures of human exposure or
risks. Communicating differences between products in machine measurements as differences in
exposure or risk is a misuse of testing using ISO standards.
INTERNATIONAL STANDARD ISO 22634-2:2017(E)
Cigarettes — Determination of benzo[a]pyrene in cigarette
mainstream smoke using GC/MS —
Part 2:
Method using cyclohexane as extraction solvent
WARNING — The use of this document can involve hazardous materials, operations and
equipment. This document does not purport to address all the safety problems associated with
its use. It is the responsibility of the user of this document to establish appropriate safety and
health practices and determine the applicability of regulatory limitations prior to use.
1 Scope
This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total
particulate matter of cigarette mainstream smoke using gas chromatography/mass spectrometry
(GC/MS) with cyclohexane as extraction solvent.
This method was validated using ISO 3308 smoking parameters and is technically compatible with
other smoking regimes.
This document provides an alternative method to that specified in ISO 22634-1, with a different clean-
up, which is less time consuming and with a reduced analytical run allowing a potential increase of
sample throughput in comparison with ISO 22634-1.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3308, Routine analytical cigarette-smoking machine — Definitions and standard conditions
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 4387, Cigarettes — Determination of total and nicotine-free dry particulate matter using a routine
analytical smoking machine
ISO 8243, Cigarettes — Sampling
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at http://www.iso.org/obp
4 Principle
— Sampling of the test cigarettes.
— Conditioning of the test cigarettes.
— Smoking of the test cigarettes according to the smoking procedure specified in ISO 4387.
— Extraction of the total particulate matter, collected on the glass-fibre filter pad, with cyclohexane.
— Clean-up procedure using solid phase extraction (SPE).
— Analytical determination of B[a]P by gas chromatography/mass spectrometry.
5 Apparatus
The usual laboratory apparatus and equipment and, in particular, the following.
5.1 Routine analytical cigarette-smoking machine, complying with the requirements of ISO 3308
and equipped for smoking in accordance with ISO 4387.
5.2 Gas chromatograph with a mass selective detector, equipped with its computerized control
and data acquisition and processing system. This system shall be able to pilot the mass spectrometer
in order to obtain chromatographic data under single ion monitoring (SIM) detec
...

ISO/TC 126/SC
Date: 2017-11
ISO/TC 126/SC /WG
Secretariat: DIN
Cigarettes — Determination of benzo[a]pyrene in cigarette
mainstream smoke using GC/MS — Part 2: Method using cyclohexane
as extraction solvent
Cigarettes — Dosage du benzo[a]pyrène dans le courant principal de la fumée de cigarettes par CG/SM —
Partie 2: Méthode utilisant du cyclohexane comme solvant d'extraction
Deleted: Document type: ¶
Document subtype: ¶
Document stage: ¶
Document language:
COPYRIGHT PROTECTED DOCUMENT
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or
utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be
requested from either ISO at the address below or ISO’s member body in the country of the
requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH‐1214 Vernier, Geneva, Switzerland
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
[e-mail]
www.iso.org
© ISO 2017 – All rights reserved
iv
Contents Page
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus . 2
6 Reagents . 2
7 Standards . 3
7.1 General . 3
7.2 Primary B[a]P‐d12 stock solution: 100 µg/ml . 3
7.3 Secondary B[a]P‐d12 spiking solution: 40 ng/ml . 3
7.4 Primary B[a]P stock solution: 100 µg/ml . 3
7.5 Secondary B[a]P stock solution: 1 000 ng/ml . 3
7.6 Working standard solutions . 3
7.7 Storage of standard solutions . 3
8 Preparation of sample . 3
8.1 Sampling . 3
8.2 Smoking . 4
8.3 Filter pad extraction . 4
8.4 Sample clean‐up . 4
8.5 Blank solution . 5
9 Determination . 5
9.1 GC/MS operating conditions . 5
9.2 Calibration . 5
9.3 Determination of B[a]P . 6
9.4 Calculation . 6
10 Repeatability and reproducibility . 6
11 Test report . 7
11.1 General . 7
11.2 Characteristic data about the cigarette . 7
11.3 Data about sampling . 7
11.4 Description of the test . 8
11.5 Test results . 8
Annex A (informative) Example of a chromatogram of a cigarette smoke extract . 9
Bibliography . 10
© ISO 2017 – All rights reserved
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national
standards bodies (ISO member bodies). The work of preparing International Standards is normally
carried out through ISO technical committees. Each member body interested in a subject for which a
technical committee has been established has the right to be represented on that committee.
International organizations, governmental and non‐governmental, in liaison with ISO, also take part in
the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all
matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
A list of all parts in the ISO 22634 series can be found on the ISO website. Deleted: This first edition of ISO 22634‐2,
together with ISO 22634‐1, cancels and replaces
ISO 22634:2008, of which it constitutes a minor
revision.¶
The main changes compared to the previous
edition are as follows:¶
— ISO 22634:2008 was separated into two
parts, ISO 22634‐1 and ISO 22634‐2;¶
— the titles of the two parts have been
modified accordingly;¶
— the text has been editorially revised.¶
© ISO 2017 – All rights reserved
iv
Introduction
No machine smoking regime can represent all human smoking behaviours: Deleted: .
— it is recommended that cigarettes also be tested under conditions of a different intensity of machine Deleted: It
smoking than those specified in this document;
Deleted: .
— machine smoking testing is useful to characterize cigarette emissions for design and regulatory Deleted: Machine
purposes, but communication of machine measurements to smokers can result in
Deleted: .
misunderstandings about differences in exposure and risk across brand;
— smoke emission data from machine measurements may be used as inputs for product hazard Deleted: Smoke
assessment, but they are not intended to be nor are they valid measures of human exposure or
risks. Communicating differences between products in machine measurements as differences in
exposure or risk is a misuse of testing using ISO standards.
© ISO 2017 – All rights reserved
iii
Cigarettes — Determination of benzo[a]pyrene in cigarette
mainstream smoke using GC/MS — Part 2: Method using
cyclohexane as extraction solvent
WARNING — The use of this document can involve hazardous materials, operations and equipment.
This document does not purport to address all the safety problems associated with its use. It is the
responsibility of the user of this document to establish appropriate safety and health practices and
determine the applicability of regulatory limitations prior to use.
1 Scope
This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total
particulate matter of cigarette mainstream smoke using gas chromatography/mass spectrometry
(GC/MS) with cyclohexane as extraction solvent.
This method was validated using ISO 3308 smoking parameters and is technically compatible with Deleted: document provides an
alternative
other smoking regimes.
Deleted: to that specified in
ISO 22634‐1, with a different clean‐up,
2 Normative references
which is less time consuming and with a
reduced analytical run allowing a
potential increase of sample throughput
The following documents are referred to in the text in such a way that some or all of their content
in comparison with ISO 22634‐1.¶
constitutes requirements of this document. For dated references, only the edition cited applies. For
NOTE This method has been
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3308, Routine analytical cigarette-smoking machine — Definitions and standard conditions Deleted: ISO 3402, Tobacco and
tobacco products — Atmosphere for
conditioning and testing¶
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 4387, Cigarettes — Determination of total and nicotine-free dry particulate matter using a routine
analytical smoking machine
ISO 8243, Cigarettes — Sampling
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at http://www.iso.org/obp
4 Principle
— Sampling of the test cigarettes.
— Conditioning of the test cigarettes.
— Smoking of the test cigarettes according to the smoking procedure specified in ISO 4387.
© ISO 2017 – All rights reserved
— Extraction of the total particulate matter, collected on the glass‐fibre filter pad, with cyclohexane.
— Clean‐up procedure using solid phase extraction (SPE).
— Analytical determination of B[a]P by gas chromatography/mass spectrometry.
5 Apparatus
The usual laboratory apparatus and equipment and, in particular, the following.
5.1 Routine analytical cigarette-smoking machine, complying with the requirements of ISO 3308
and equipped for smoking in accordance with ISO 4387.
5.2 Gas chromatograph with a mass selective detector, equipped with its computerized control
and data acquisition and processing system. This system shall be able to pilot the mass spectrometer in
order to obtain chromatographic data under single ion monitoring (SIM) detection mode. The gas
chromatograph shall be configured to perform splitless injections on a capillary column. It is
recommended to equip the gas chromatograph with an autosampler for sample injection.
5.3 Fused silica capillary column, for example a 50 % phenyl‐, 50 % methyl‐polysiloxane
stationary phase and a 30 m length, 0,25 mm internal diameter column with a 0,25 μm film thickness
are suitable for this analysis.
NOTE Other columns can be used, provided that appropriate peak separation is obtained.
5.4 TurboVap® evaporator or equivalent equipment.
5.5 Vacuum sample preparation unit or equivalent equipment.
5.6 Solid phase extraction cartridges, NH bonded silica phase volume of 3 ml and packed wi
...

NORME ISO
INTERNATIONALE 22634-2
Première édition
2017-11
Cigarettes — Dosage du benzo[a]
pyrène dans le courant principal de la
fumée de cigarette par CG/SM —
Partie 2:
Méthode utilisant du cyclohexane
comme solvant d'extraction
Cigarettes — Determination of benzo[a]pyrene in cigarette
mainstream smoke using GC/MS —
Part 2: Method using cyclohexane as extraction solvent
Numéro de référence
©
ISO 2017
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2017, Publié en Suisse
Droits de reproduction réservés. Sauf indication contraire, aucune partie de cette publication ne peut être reproduite ni utilisée
sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique, y compris la photocopie, l’affichage sur
l’internet ou sur un Intranet, sans autorisation écrite préalable. Les demandes d’autorisation peuvent être adressées à l’ISO à
l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. [phone]
Fax [phone]
[e-mail]
www.iso.org
ii © ISO 2017 – Tous droits réservés

Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe . 2
5 Appareillage . 2
6 Réactifs . 3
7 Étalons . 3
7.1 Généralités . 3
7.2 Solution mère primaire de B[a]P-d12: 100 µg/ml . 3
7.3 Solution de dopage secondaire de B[a]P-d12: 40 ng/ml . 3
7.4 Solution mère primaire de B[a]P: 100 µg/ml . 3
7.5 Solution mère secondaire de B[a]P: 1 000 ng/ml . . 3
7.6 Solutions étalons de travail . 3
7.7 Stockage des solutions étalons . 4
8 Préparation de l’échantillon . 4
8.1 Échantillonnage . 4
8.2 Fumage . 4
8.3 Extraction des filtres . 4
8.4 Purification de l’échantillon. 4
8.5 Essai à blanc . 5
9 Dosage . 5
9.1 Conditions de fonctionnement CG/SM . 5
9.2 Étalonnage . 6
9.3 Dosage du B[a]P . 6
9.4 Calcul . 6
10 Répétabilité et reproductibilité . 6
11 Rapport d’essai . 7
11.1 Généralités . 7
11.2 Données caractéristiques concernant la cigarette . 7
11.3 Données concernant l’échantillonnage . 8
11.4 Description de l’essai . 8
11.5 Résultats de l’essai . 8
Annexe A (informative) Exemple de chromatogramme d’un extrait de fumée de cigarette .9
Bibliographie .10
Avant-propos
L'ISO (Organisation internationale de normalisation) est une fédération mondiale d'organismes
nationaux de normalisation (comités membres de l'ISO). L'élaboration des Normes internationales est
en général confiée aux comités techniques de l'ISO. Chaque comité membre intéressé par une étude
a le droit de faire partie du comité technique créé à cet effet. Les organisations internationales,
gouvernementales et non gouvernementales, en liaison avec l'ISO participent également aux travaux.
L'ISO collabore étroitement avec la Commission électrotechnique internationale (IEC) en ce qui
concerne la normalisation électrotechnique.
Les procédures utilisées pour élaborer le présent document et celles destinées à sa mise à jour sont
décrites dans les Directives ISO/IEC, Partie 1. Il convient, en particulier de prendre note des différents
critères d'approbation requis pour les différents types de documents ISO. Le présent document a été
rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir www.
iso.org/directives).
L'attention est attirée sur le fait que certains des éléments du présent document peuvent faire l'objet de
droits de propriété intellectuelle ou de droits analogues. L'ISO ne saurait être tenue pour responsable
de ne pas avoir identifié de tels droits de propriété et averti de leur existence. Les détails concernant
les références aux droits de propriété intellectuelle ou autres droits analogues identifiés lors de
l'élaboration du document sont indiqués dans l'Introduction et/ou dans la liste des déclarations de
brevets reçues par l'ISO (voir www.iso.org/brevets).
Les appellations commerciales éventuellement mentionnées dans le présent document sont données
pour information, par souci de commodité, à l’intention des utilisateurs et ne sauraient constituer un
engagement.
Pour une explication de la nature volontaire des normes, la signification des termes et expressions
spécifiques de l'ISO liés à l'évaluation de la conformité, ou pour toute information au sujet de l'adhésion
de l'ISO aux principes de l’Organisation mondiale du commerce (OMC) concernant les obstacles
techniques au commerce (OTC), voir le lien suivant: www.iso.org/avant-propos.
Le présent document a été élaboré par le comité technique ISO/TC 126, Tabac et produits du tabac.
Une liste de toutes les parties de la série de normes ISO 22634 est disponible sur le site Internet de l’ISO.
iv © ISO 2017 – Tous droits réservés

Introduction
Aucun régime de fumage mécanique ne peut reproduire parfaitement le comportement des fumeurs:
— il est recommandé que les cigarettes soient aussi soumises à essai sous les conditions d’une différente
intensité de fumage mécanique que celles spécifiées dans le présent document;
— l’essai de fumage mécanique permet de caractériser les émissions des cigarettes pour des besoins
de conception et de réglementation, mais communiquer aux fumeurs les résultats de mesures sur
machines peut susciter des malentendus sur les niveaux d’exposition et de risque selon les marques;
— les données concernant l’émission de fumées obtenues par des mesurages mécaniques peuvent
être utilisées comme des données de base pour l’évaluation des dangers du produit, mais elles ne
constituent pas des mesures valables de l’exposition des individus et des risques et ce n’est pas
leur but. C’est faire un usage impropre des essais réalisés conformément aux Normes ISO que de
communiquer que les différences de mesures mécaniques entre différents produits correspondent
à des différences de niveaux d’exposition ou de risque.
NORME INTERNATIONALE ISO 22634-2:2017(F)
Cigarettes — Dosage du benzo[a]pyrène dans le courant
principal de la fumée de cigarette par CG/SM —
Partie 2:
Méthode utilisant du cyclohexane comme solvant
d'extraction
AVERTISSEMENT — L’utilisation du présent document peut impliquer l’utilisation de produits
et la mise en œuvre de modes opératoires et d’appareillages à caractère dangereux. Le
présent document n’a pas pour but de traiter tous les problèmes de sécurité qui sont liés à
son utilisation. Il incombe à l’utilisateur du présent document d’établir, avant de l’utiliser, des
pratiques d’hygiène et de sécurité appropriées et de déterminer l’applicabilité des restrictions
réglementaires.
1 Domaine d’application
Le présent document spécifie une méthode de dosage du benzo[a]pyrène (B[a]P) dans la matière
particulaire totale du courant principal de la fumée de cigarette par chromatographie en phase gazeuse
couplée à la spectrométrie de masse (CG/SM) en utilisant du cyclohexane comme solvant d’extraction.
Cette méthode a été validée en utilisant les paramètres de fumage de l’ISO 3308, et elle est techniquement
compatible avec d’autres régimes de fumage.
Le présent document définit une méthode alternative à l’ISO 22634-1, avec un nettoyage différent,
moins chronophage et avec une série d’analyses réduite, qui permet une augmentation potentielle de la
cadence des échantillons par comparaison à l’ISO 22634-1.
2 Références normatives
Les documents suivants cités dans le texte constituent, pour tout ou partie de leur contenu, des
exigences du présent document. Pour les références datées, seule l’édition citée s’applique. Pour les
références non datées, la dernière édition du document de référence s'applique (y compris les éventuels
amendements).
ISO 3308, Machine à fumer analytique de routine pour cigarettes — Définitions et conditions normalisées
ISO 3402, Tabac et produits du tabac — Atmosphère de conditionnement et d'essai
ISO 4387, Cigarettes — Détermination de la matière particulaire totale et de la matière particulaire
anhydre et exempte de nicotine au moyen d'une machine à fumer analytique de routine
ISO 8243, Cigarettes — Échantillonnage
3 Termes et définitions
Aucun terme n’est défini dans le présent document.
L’ISO et l’IEC tiennent à jour des bases de données terminologiques destinées à être utilisées en
normalisation, consultables aux adresses suivantes:
— IEC Electropedia: disponible à l’adresse http://www.electropedia.org/;
— ISO Online browsing platform: disponible à l’adresse http://www.iso.org/obp.
© ISO
...